ONE Broth-Listeria data sheet - Oxoid

ONE Broth–Listeria
ONE Broth–Listeria
ONE Broth–Listeria
Simple
Culture Media
h–Listeria ONE Broth–Listeria
ONE Broth–Listeria
ONE Bro
ONE Broth–Listeria
–Listeria ONE Broth–Listeria
No subculturing from primary to secondary broths. Simply incubate for 24 hours and subculture
onto agar medium
Fast
At 24 hours ONE Broth gave comparable recovery of Listeria compared to a conventional
two broth, 48 hour method 1,2,3.
Improved Recovery
Higher levels of Listeria were recovered from samples when compared to the ISO method 2,3
Efficient
A single broth instead of two, means savings on time and materials
Listeria Precis
ONE Broth
ONE Broth–Listeria
ONE Broth–Listeria
A novel broth for the complete selective enrichment of Listeria
species from food samples in 24 hours.
ONE Broth–Listeria is the enrichment broth used in the AFNOR validated Listeria Precis
method

ONE Broth–Listeria
This novel enrichment broth incorporates a carefully balanced mixture of
peptones, carbohydrates and salts to give optimal resuscitation, recovery
and growth of even low numbers of Listeria spp. from food samples after
24 hours.
Recovery of Listeria spp. after 24 hours using Oxoid Novel Enrichment
Broth–Listeria (ONE Broth–Listeria) has been shown to be comparable
and in some cases better than using the ISO 11290-11 combined Fraser
and Half Fraser broth enrichment procedure2 . Convenient freeze-dried
vials provide easy addition of selective agents to the broth base allowing
recovery of Listeria spp. while inhibiting a wide range of competing fl ora.
Internal trials and an evaluation carried out on behalf of Oxoid at
Leatherhead Food International show that at 24 hours ONE Broth–Listeria is
capable of the improved recovery of Listeria when compared to Half Fraser
Broth alone or Half Fraser followed by full Fraser Broth. In addition, the
number of Listeria cells detected after broth enrichment for 24 hours was
found to be higher with ONE Broth–Listeria than with Half Fraser Broth.
ONE Broth–Listeria offers the benefi t of requiring a single enrichment
medium at one temperature, with no need to sub-culture into a secondary
enrichment broth. Listeria recovery levels at 24 hours are at least equivalent
to the ISO enrichment method for and in some cases are improved2,3 .
Protocol for Listeria Precis Method
Day 0: Enrichment
25g or 25mL of sample + 225mL ONE Broth–Listeria
Incubate for 24 ± 2 hours at 30°C
Day 1: Plating
Using a 10µL microbiological loop inoculate a single
Brilliance Listeria plate. Incubate for 22–26 hours at 37°C
(for meat samples,
re-incubate plates that
show no blue colonies
with halos for a further
22–26 hours at 37°C)
DEDICATED TO MICROBIOLOGY
Select green-blue
colonies with halos
for confirmation
Day 2: Results
If present, confirm blue/green colonies with halos as
L. monocytogenes using the O.B.I.S. mono test. Alternatively,
confirm using standard ISO methods.**
For Listeria monocytogenes enumeration:
Resuscitate any organisms present in the sample by adding 25g or 25mL to
225mL of Buffered Peptone Water and incubate for 1 hour at 20°C. Inoculate
a single Brilliance Listeria plate with 100µL, and incubate for 45–51 hours at
37°C. Inspect the plate for characteristic blue/green colonies with halos and
count. Confirm using O.B.I.S. mono or alternatively, confirm using standard ISO
methods.** Calculate CFU/g or CFU/mL of sample.
**If there is insufficient material to carry out an O.B.I.S. mono test, or if a mixed culture of
L. monocytogenes and other Listeria species is suspected, first purify suspect colonies by subculture
onto a second Brilliance Listeria plate.
Directions
Suspend 22 grams of ONE Broth–Listeria Base in 500mL of distilled water. Mix well and sterilize by
autoclaving at 121°C for 15 minutes. Cool the medium to below 50°C, and aseptically add the contents of
one vial of ONE Broth–Listeria Selective Supplement reconstituted as directed.
Method of Use
1. Add 25g of food sample to 225mL of ONE Broth–Listeria and stomach for a minimum of 30 seconds to
mix the sample.
2. Incubate the broth without agitation at 30°C for 24 ± 2 hours.
3. Gently agitate the bag then, using a microbiological loop, remove 10µL and inoculate onto a suitable
agar medium e.g. Brilliance Listeria Agar and incubate appropriately (37°C for 24–48 hours).
4. Confirm presumptive colonies on the agar plates as Listeria monocytogenes or Listeria spp.
Enrichment/resusitation media
ONE Broth–Listeria in Bottles 10x225mL BO1066S*
ONE Broth–Listeria in ReadyBags 3x3 litres FR60031*
ONE Broth–Listeria Base 500g CM1066B
ONE Broth–Listeria Supplement for 500mL 10 vials SR0234E
ONE Broth–Listeria Supplement for 2.25 litres 10 vials SR0234B
Buffered Peptone Water (ISO) in Bottles 10x225mL BO1067S*
Buffered Peptone Water (ISO) in ReadyBags 4x3 litres BM1104T*
Buffered Peptone Water 500g CM0509B
Plating media
Brilliance Listeria (ready-to-use 90mm plates) 10 plates PO1102A*
Brilliance Listeria Agar Base 500g CM1080B
Brilliance Listeria Agar Base 2 litre pack 134.4g CM1080E
Brilliance Listeria Selective Supplement for 500mL 10 vials SR0227E
Brilliance Listeria Differential Supplement for 500mL 10 vials SR0228E
Confirmation
Oxoid Listeria Latex Test Kit 100 tests DR1126A
O.B.I.S. mono Kit 60 tests ID0600M
Microbact 12L 20 tests MB1128A
Remel RapID CB Plus Panel 20 panels R8311008*
Gram Stain Kit 4x250mL R40080*
Microbact Oxidase Strips 50 tests MB0266A
Tryptone Soya Broth 500g CM0129B
Yeast Extract Powder 500g LP0021B
Agar Bacteriological 500g LP0011B
Blood Agar Base No. 2 500g CM0271B
Defibrinated Sheep Blood 25mL SR0051B*
Quality Control Organisms – Culti-Loops TM
SIZE/FORMAT ORDER CODE
Listeria monocytogenes ATCC ® 7644 † 5 loops CL3970*
Staphylococcus aureus ATCC ® 25923 † 5 loops CL7010*
Listeria innocua ATCC ® 33090 † 5 loops CL9005*
Escherichia coli ATCC ® 25922 † 5 loops CL7050*
Enterococcus faecalis ATCC ® 29212 † 5 loops CL7030*
Rhodococcus equis ATCC ® 6939 † 5 loops CL5400*
References:
1. ISO 11290-1 (Microbiology of food and animal feeding stuffs – Horizontal method
for the enumeration of Listeria monocytogenes – part 1).
2. Oxoid Poster - Folio No. 1033, July 2004.
3. Data on file at Oxoid.
Oxoid and Remel are specialty microbiology brands of Thermo Fisher Scientifi c. Our products are available worldwide.
www.oxoid.com
Tel: +44 (0) 1256 841144
oxoid.info@thermofi sher.com
†
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Folio No LT1417A/MS/12/10