27362 oxoid salmonella precis

monella m
Salmonella
Salmo
Rapid Culture Method
A quick and easy method for the enrichment, detection and
confirmation of Salmonella species from food, animal feed and
environmental samples.
� Validated by AFNOR to ISO 16140 standard
� Simple and easy procedure – no specialized equipment required
� Single 18-hour enrichment
� Single sample transfer
� Single 24-hour plate incubation
� Quick and convenient confirmation: Oxoid Salmonella Latex Test or
ISO 6579:2002 standard tests
� Reduced time to result: 2 days compared with up to 5 days for
standard culture methods
AFNOR

Introduction
The Oxoid Salmonella Precis method combines the benefits
of ONE Broth-Salmonella, Brilliance Salmonella Agar and
the Oxoid Salmonella Latex Test to reduce time to result over
conventional culture methods.
ONE Broth-Salmonella is a highly nutritious medium for the
recovery and growth of salmonellae while inhibiting competing
organisms. The growth promoter in the medium allows the
recovery of stressed Salmonella cells, even when present in very
low numbers.
Brilliance Salmonella Agar is the first in a new class of
chromogenic media to incorporate novel Inhibigen technology.
This new technology improves recovery of Salmonella by
reducing background flora. Chromogens aid easy identification
and differentiation by producing brightly coloured colonies.
The Oxoid Salmonella Latex Test provides a quick and easy
method for confirmation of Salmonella species from culture
media.
Protocol for Salmonella Precis Method
Day 0: Enrichment
25g or 25mL of sample + 225mL ONE Broth-Salmonella
Incubate for 16–20 hours at 42°C
Day 1: Plating
Using a 10µL microbiological loop inoculate a single
Brilliance Salmonella plate. Incubate for 22–26 hours at 37°C
Day 2: Results
If present, select a well isolated purple coloured colony and test
using the Oxoid Salmonella Latex Test. Alternatively, confirm
purple colonies using standard ISO methods.
Select purple colonies for confirmation
DEDICATED TO MICROBIOLOGY
AFNOR Validation
The Salmonella Precis method has been validated and approved by AFNOR according
to ISO 16140 standard against the reference method ISO 6579:2002 standard for the
detection of Salmonella in food, animal feed and environmental samples.
For flexibility, confirmation was validated using both Oxoid Salmonella Latex Test
and the tests outlined in ISO 6579:2002. Alternatively, biochemical panels such as
Microbact GNB 24E or Remel RapID ONE Panel, may be used.
AFNOR validation certificate number UNI 03/06 – 12/07 (available in PDF format from
the AFNOR website www.afnor-validation.com).
Reactions on Brilliance Salmonella Agar
†
Enzyme
targeted by
chromogen
Colony colour
Purple Blue Colourless
Salmonella
(including Lactose
positive Salmonella)
Klebsiella,
Enterobacter,
Serratia
Citrobacter,
other bacteria
and yeasts
Esterase + -/+ -
ß-glucosidase - + -
E. coli and other bacteria and yeasts are inhibited by the combination
of Inhibigen and other selective agents in the medium.
Enrichment media SIZE/FORMAT ORDER CODE
ONE Broth-Salmonella in Bottles 10x225mL BO1096S*
ONE Broth-Salmonella in ReadyBags 3x3 litres FR60101*
ONE Broth-Salmonella Base 500g CM1091B
ONE Broth-Salmonella Supplement for 225mL 10 vials SR0242E
ONE Broth-Salmonella Supplement for 2.25 litres 10 vials SR0242B
Plating media
Brilliance Salmonella (ready to use 90mm plates) 10 plates PO5098A*
Brilliance Salmonella Agar Base 500g CM1092B
Salmonella Selective Supplement for 500mL 10 vials SR0194E
Confirmation
Oxoid Salmonella Latex Test 30 tests FT0203A
Nutrient Agar 500g CM0003B
Microbact GNB 24E 40 tests MB1131A
Microbact GNB 24E 80 tests MB1074A
Remel RapID ONE Panel 20 panels R8311006*
Triple Sugar Iron Agar 500g CM0277B
Urea Agar Base 500g CM0053B
Urea 40% Solution (for 100mL medium) 10x5mL SR0020K
Lysine Decarboxylation Broth Tablets (each make 5ml) 100 tablets CM0308S
Spot Indole Reagent (DMACA) 25mL R21245*
Remel Salmonella O and H Agglutinating Sera various codes - see Product List
Quality Control Organisms – Culti-Loops TM
Salmonella Typhimurium ATCC ® 14028 † 5 loops CL6000*
Staphylococcus aureus ATCC ® 25923 † 5 loops CL7010*
Klebsiella pneumoniae ATCC ® 13883 † 5 loops CL7037*
Enterococcus faecalis ATCC ® 29212 † 5 loops CL7030*
Escherichia coli ATCC ® 25922 † 5 loops CL7050*
The ATCC Licensed Derivative Emblem ® , the ATCC Licensed Derivative word mark ® , and the ATCC catalog marks are
trademarks of ATCC. Oxoid Ltd is licensed to use these trademarks and sell products derived from ATCC ® cultures.
* Check code and availability with your local Oxoid Representative
For more information about these products, please visit www.oxoid.com
Oxoid and Remel are specialty microbiology brands of Thermo Fisher Scientifi c. Our products are available worldwide.
www.oxoid.com
Tel: +44 (0) 1256 841144
oxoid.info@thermofi sher.com
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Folio No 1215/MS/11/10