Proposal - Oncourse - Indiana University

the oral cavity where intake occurs. This can lead to multiple diseases including periodontal
disease, which involves the degradation of collagen. This team members’ study (Voiles)
examined the effects that nicotine and cigarette smoke condensate (CSC) have on type I collagen
mRNA expression in human gingival fibroblasts (HGFs) by Polymerase Chain Reaction (PCR).
Collagen expression was slightly increased in the nicotine and the CSC exposed HGFs in
comparison to the untreated control. Future experiments will examine additional HCF cell lines
in order to confirm these results.
Song: Eric Grow has studied on the effect of smoke condensate on human pulp cells for one and
half years. His latest research activity with our MURI team is to detect and analysis the cytokine
expression under the influence of cigarette smoke condensate using cytokine array kit from Bio-
Ray technology. Due to the time limitation, the study using the green tea extract against smoke
condensate is initiated but need more time to advance further. Eric will volunteer to work in the
lab this coming summer and finish up the project. A manuscript regarding this research effort is
under construction.
MURI Mentor’s Project Proposal Form, Updated: 1-25-2011 4