Congenital Cytomegalovirus Conference - Congenital CMV ...

Results: We were able to classify the CMV strains in 11 groups (6 prototypic strains and 5 recombinant)
for UL55, 2 for UL75, 4 main types and 3 subtypes for UL73, which distinguishes a maximum of 154
different strains. All genotypes were encountered in most of the DCCs and the EU. In two DCC’s all
the children were infected by the same genotype for UL55, UL73 and UL75; 37 children (17%) harbored
multiple strains, 28 in DCC’s, 9 in the EU, whereas no coinfection was detected in newborn isolates.
Recombinants strains (15%) were observed with reproducible profiles in the different populations and
different sampling sites.
Conclusion: This method classify CMV strains more precisely than previous RFLP-based classifications,
and could constitute the basis of a new classification, particularly for gpUL55, may be more closely
related to CMV behaviour. Easy identification of co-infections and recombinants directly from
pathological samples, could help large scale epidemiologic studies.
O-08 CMV congenital infection in children born to HIV infected mothers over a 10 years period (1993-2004).
Marianne Leruez-Ville, Gaelle Guibert, Jérome Le Chenadec, Stéphane Blanche, Laurent Mandelbrot,
Roland Tubiana, Christine Rouzioux, Josiane Warszawski. Hospital Necker-Enfants Malades, Paris, France.
Background: There are few data available concerning the burden of CMV congenital infection in children
born to HIV infected mothers.
Objectives: To evaluate the prevalence and the maternal risk factors for cytomegalovirus (CMV)
congenital infection in children born to HIV infected mothers. CMV congenital infection was studied in
children born alive between 1993 and 2004 and enrolled in the French Perinatal Cohort (EPF), a national
prospective multicenter cohort of mother-to-child HIV transmission.
Methods: CMV congenital infection was screened by rapid culture (1993 to 2001) and rapid culture or real
time PCR (since 2001) in a urine sample obtained within the ten first days of life. Maternal CMV serology
was recorded in EPF files until 2001 and 91.9% of mothers were seropositive for CMV.
Results: CMV neonatal screening was performed for 5019 of the 7563 newborns included in EPF (1993 to
2004). The overall prevalence of CMV infection was 2.3% (95% CI:1.9-2.8), this prevalence was higher in
HIV infected newborns (10.3%; 95% CI: 5.6-17.0) than in HIV uninfected newborns (2.2%, 95% CI:1.8-2.7),
p