CURRICULUM VITAE

CURRICULUM VITAE 

Updated on Jan. 2012 

PERSONAL DATA 

Name: 

ARASH REZA MEMARNEJADIAN 

Date of Birth: May 15, 1975 

Place of Birth: Tehran / Iran 

Gender: 

Male 

Nationality: Iranian 

Native Language: Persian 

Marital Status: Married with two daughters 

Work Address: Hepatitis & AIDS Dept., Pasteur institute of Iran, Pasteur Ave., 

Tehran 1316943551, Iran. 

Tel / Fax: +98.21.66969291 

Mobile Phone: +98.9124494055 

E-mail: 

memarnejad@pasteur.ac.ir 

memarnejadian@gmail.com 

EDUCATION 

2003 to 2009 Ph.D. in Medical Biotechnology, Pasteur institute, Tehran, Iran, (GPA: 

19.10 out of 20, Top ranked student in the class). 

Advanced course experiences during Ph.D.: 

Molecular genetics, Advanced genetic engineering, Advanced 

immunology, Advanced biochemistry, Industrial microbiology, Vaccine 

production, Pharmaceutical biotechnology. 

Thesis title: “Designing, Construction and Evaluation of a Poly-epitope 

DNA Construct Using Immunodominant Hepatitis C Virus Epitopes”. 

Supervisors: Dr. Farzin Roohvand (from Pasteur institute of Iran) and Dr. 

Behazine Combadière (from Université Pierre et Marie Curie, Paris, 

France).

A. R. Memarnejadian-Curriculum Vitae 

2000 to 2003 M.Sc. in Medical Biotechnology, Tarbiat Modarres Univ., Tehran, Iran, 

(GPA: 18.75 out of 20, Top ranked student in the class). 

Advanced course experiences during M.Sc.: 

Bioinformatics, Human genetics, Animal cell culture, Genetic 

engineering, Working with Laboratory animals and Advanced techniques 

in biotechnology. 

Thesis title: “Cloning and Evaluation of the Expression for Streptokinase 

Gene with a 6xHis Epitope in E. coli M15”. 

Supervisor: Dr. Farzin Roohvand. 

1997 to 1999 B.Sc. in Medical Lab. Sciences, Iran Univ. of Medical Sciences, Tehran, 

Iran, (GPA: 18.81 out of 20, Top ranked student in the class). 

1994 to 1996 Associate Diploma in Medical Lab. Sciences, Shahid Beheshti Univ. of 

Medical Sciences, Tehran, Iran, (GPA: 18.80 out of 20, Top ranked 

student in the class). 

1990 to 1994 Diploma in Experimental Sciences, Dehkhoda High School, Karaj, Iran, 

(GPA: 18.99 out of 20, Top ranked student in high school) 

Awards and Honors: 

1. The Award of Excellence from the Pasteur Institute vice chancellor for research (in 

recognition of top graduates in Ph.D. program), 2010. 

2. Chinese academy of science and Pasteur institute of China travel award to participate in 

“Antiviral Immunity Course, 2010”. The award was declined by the applicant. 

3. “HIV Research Trust” organization (UK) scholarship to attend a 2-month training on HIV 

drug-resistance detection in Center for AIDS Research, Education and Support (CARES), 

Nagoya, Japan (2010). 

4. Pasteur institute of Iran Post-Doctoral fellowship (2009-2010) 

5. Achievement of excellent score (20 out of 20) for Ph.D. thesis (Pasteur institute of Iran, 

2009). 

6. Graduate opportunity program fellowship from Iran Ministry of Health Dec. 2007-Jun. 

2008, Paris, France. 

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7. International scholarship from OIC standing committee on scientific and technological 

cooperation (COMSTECH) for participation in international thematic workshop on 

“DNA & Emerging Vaccines and Production Challenges” in Islamabad, Pakistan, 3 rd -9 th 

Sept 2007. 

8. Full scholarship from Pasteur Institute of Iran for Ph.D. program, 2003-2007. 

9. Top ranked student among the participants in the national entrance exam for Medical 

Biotechnology Ph.D. program (2003). 

10. Second ranked student among the participants in the national entrance exam for Medical 

Biotechnology M.Sc. program (2000). 

11. Top ranked student among the participants in the national entrance exam for Immunology 

M.Sc. program (2000), (Education in this field was declined by the participant). 

12. Top ranked student among the participants in the national entrance exam for Medical 

Lab. Sciences B.Sc. program (1997). 

13. The Award of Excellence from the then President of Iran (in recognition of top graduates 

of undergraduate program in Shahid Beheshti Uni. of Medical Sciences), 1997. 

Postdoctoral Fellowship and Trainings: 

1. Practical training on “HIV Molecular Epidemiology and Drug-resistance Detection”, Oct. 

15 th to Dec. 2 nd , 2010, Department of Infection and Immunology, Center for AIDS 

Research, Education and Support (CARES), National Hospital Organization, Nagoya 

Medical Center, Nagoya, Japan, Supervisor: Prof. Wataru Sugiura. 

2. Postdoctoral fellow in viral immunology and biotechnology, Mar. 2009 to Mar. 2010, 

Hepatitis & AIDS Dept., Pasteur Institute, Tehran, Iran, Mentors: Dr. S. Amini 

(Associate Professor) and Dr. M. Aghasadeghi (Assistant Professor). 

3. Practical trainings on vaccine research and immune response assessment techniques, Dec. 

2007 to Jun. 2008, Laboratoire d’Immunologie - INSERM U945, Université Pierre et 

Marie Curie, Paris, France, Supervisor: Dr. B. Combadière (Associate Professor). 

4. International thematic workshop on “DNA & Emerging Vaccines and Production 

Challenges”. Organized by COMSTECH and HEC, 3 rd -9 th Sept. 2007, COMSTECH 

Secretariat, Islamabad, Pakistan. 

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5. International training course on Bioinformatics. 4 th -10 th Sept. 2004, Institute of 

biochemistry and biophysics, University of Tehran, Tehran, Iran. 

Professional Affiliations: 

1. Apr. 2010 to present, Assistant Professor and researcher in hepatitis & AIDS Dept., 

Pasteur institute, Tehran, Iran. 

2. Mar. 2009 to Mar. 2010, Postdoctoral research fellow in hepatitis & AIDS Dept., Pasteur 

institute, Tehran, Iran. 

3. 2003-2009, Ph.D. candidate and research assistant in Pasteur institute, Tehran, Iran. 

4. 1999-2003, Medical Lab. technologist (Head of the shift) in Loghman hospital, Shahid 

Beheshti Univ. of Medical Sciences, Tehran, Iran. 

5. 1996-1999, Medical Lab. technician in Shohaday-e Tajrish hospital, Shahid Beheshti 

Univ. of Medical Sciences, Tehran, Iran. 

Research and Technical Skills: 

1. Basic molecular biology and recombinant DNA technology (DNA/RNA extraction, 

cloning, PCR, RT-PCR, site-directed mutagenesis, PCR-RFLP, SOEing PCR). 

2. Recombinant protein expression in bacteria and mammalian cells 

3. Protein purifications 

4. Western-blot analysis 

5. Mammalian cell culture (adherent and suspension) 

6. Stable and transient transfection of mammalian cells 

7. Humoral immune response analysis (ELISA, Radio-immunoassay, Immunofluorescence) 

8. Cellular immune response analysis (in vivo and in vitro cytotoxicity assay, ELISpot 

assay, proliferation assay) 

9. Flowcytometry 

10. Molecular epidemiology and phylogenetic tree analysis 

11. Bioinformatics (DNA and protein alignments, Blast analysis, drawing plasmid maps, 

finding ORF, restriction site analysis, protein secondary structure analysis) 

12. In silico immunology (antigenic peptide determination, CTL and Th epitope mapping, 

MHC-binding affinity determination, proteasomal cleavage site analysis) 

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13. Handling and working with Lab. animals (bleeding, injection, euthanasia) 

14. Medical Lab. diagnosis tests (Biochemistry, Hematology, Serology, Microbiology) 

Research Interests: 

1. Viral immunology and vaccine design against HCV and HIV 

2. Molecular epidemiology and drug resistance studies on HIV 

3. Application and engineering of viral vectors as vaccine vehicles 

4. Recombinant protein expression in prokaryotic/eukaryotic systems 

Current Positions in Research Projects 

I) Principal Investigator and Grant Holder in: 

1. “Study on the periplasmic expression of human interferon-α2b in Escherichia coli in 

order to produce an expression vector with applicability in the pharmaceutical industry”, 

Supported by Shahid Beheshti Uni. of Medical Sciences, Tehran, Iran. 

2. “Determining HIV subtypes and drug resistance mutations among infected individuals 

from various HIV high risk population in Tehran province”, Supported by Pasteur 

institute of Iran. 

3. “Construction of engineered adenoviral vector for effective induction of anti-HIV 

humoral response”, Supported by Pasteur institute of Iran. 

II) Supervisor / Advisor of Ph.D. and M.Sc. Theses: 

1. “Determining HIV-1 subtypes and drug resistance mutations in HIV-1 infected patients 

from different high-risk groups in Tehran”, Microbiology M.Sc. theis, Azad Uni. Qom 

branch, Qom, Iran. 

2. “Genetic variation of HIV‐1 integrase region in newly‐diagnosed integrase 

inhibitor‐naïve patients with HIV/AIDS in Iran”, Microbiology M.Sc. thesis, Azad Uni. 

Karaj branch, Karaj, Iran. 

3. “Designing, Construction and Evaluation of Multiepitope DNA/Protein vaccines against 

HIV-1”, Molecular and cellular biology M.Sc. thesis, Azad Uni. Research and 

Technology branch, Tehran, Iran. 

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4. “Designing and Construction of Recombinant pIPIGFP Plasmid Expressing HIV-1 

Virions as Vaccine Carrier”, Genetics M.Sc. thesis, National Institute for Genetic 

Engineering and Biotechnology, Tehran, Iran. 

5. “Designing and Construction of HCV Multiepitope Experimental Vaccine and Evaluation 

of Its Immunogenicity in Mouse Model”, Virology Ph.D. thesis, Tehran Uni. of Medical 

Sciences, Tehran, Iran. 

6. "Study on the Effect of HCV Capsid Protein Expression on Transcriptional Levels of 

IRF-1, ISG20 and ISG56 Genes in HepG2 Cell Line in the Presence or Absence of α- 

interferon", Pharmaceutical biotechnology Ph.D. thesis, Pasteur institute, Tehran, Iran. 

7. "Evaluation of Responses towards H-2 d Core Epitope of HCV in BALB/c Mice 

Immunized by Recombinant BCGs Harboring the Native Gene or a Polytopic Construct", 

Pharmaceutical biotechnology Ph.D. thesis, Pasteur institute, Tehran, Iran. 

8. "Cloning and Expression of Streptokinase Gene from Iranian Streptococci Strains: 

Genetic Analysis and Enzymatic Assessment", Pharmaceutical biotechnology Ph.D. 

thesis, Pasteur institute, Tehran, Iran. 

9. "Construction of a Recombinant BCG that Secretes Multuepitope Containing E2, Core 

and NS3 Epitopes to Achieve an Efficient and Safe HCV Vaccine", Cellular and 

molecular biology M.Sc. thesis, Khatam Univ., Tehran, Iran. 

III) Co-investigator in Granted Projects: 

1. "Immunogenicity and Production of Poly/Monoclonal Antibodies against ARFP/F 

(core+1) Protein of HCV by Utilization of Epitopic-Synthetic Peptides & Core+1 

Recombinant Protein-Evalution of New Human Compatible Adjuvants and Different 

Myeloma Cells", Pasteur institute of Iran (Project 391). 

2. "Evaluation of Immune Responses to Immunization of Th/CD8+ Epitopic Peptides 

Alone, Mixed or in Polyepitopic-Peptides Related to HCV Proteins in BALB/c Mice", 

Pasteur institute of Iran (Project 393). 

3. "Comparative Study on Mutations Associated with Lamivudine Resistance in HBV and 

HBV/HIV Co-Infected Patients", Pasteur institute of Iran (Project 413). 

4. "Design and Expression of a Mouse/Human Chimeric Anti-HCV NS3 Monoclonal 

Antibody", Pasteur institute of Iran (Project 460). 

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5. "Threshold Survey Evaluating Transmitted HIV Drug Resistance Mutations of Pro and 

RT Genes in Iran 2009", WHO and CDC joint project (Contract ID APW.09/01907). 

6. "Preparation of National Guideline for HIV-DR Threshold Survey", WHO and CDC joint 

project (Contract ID APW/09/00233). 

7. "Designing, Constructing and Evaluation of Bicistronic Plasmid Capable of Simultaneous 

Expression of Antigen and IL18/Ig Adjuvant", Pasteur institute of Iran (Project 478). 

8. “Construction, expression and immunological evaluation of mzNL4-3-NS3 in BALB/c 

mice”, Pasteur institute of Iran (Project 673). 

Teaching Experiences: 

1. Full lecturer in “Advanced Virology” theoretical course for M.Sc. students of 

Microbiology, Azad Uni., Karaj branch, Karaj, autumn 2011. 

2. Full lecturer in “Pharmaceutical Biotechnology” theoretical course for M.Sc. students of 

Biotechnology, Alzahra Uni., Tehran, autumn 2011. 

3. Full lecturer in “Medical Biotechnology” theoretical course for B.Sc. students of 

Biotechnology, Alzahra Uni., Tehran, autumn 2011. 

4. Full lecturer and instructor in “Computer Applications in Biotechnology” 

theoretical/practical course for M.Sc. students of Microbial Biotechnology, Payame Noor 

Uni., Tehran, autumn 2011. 

5. Full lecturer in “Molecular Genetics of Bacteria” theoretical course for M.Sc. students of 

Microbial Biotechnology, Payame Noor Uni., Tehran, autumn 2011. 

6. Lecturer (2 hrs, with the title of “Human Endogenous Retroviruses: Their Role in Health 

and Disease”) in Advanced Molecular Biology course for Ph.D. students of Medical 

Biotechnology, Pasteur institute of Iran, December 2011. 

7. Lecturer (2 hrs, with the title of “Second and Third Generation Vaccines”) in 

Vaccinology course for Ph.D. students of Medical Biotechnology, Pasteur institute of 

Iran, March 2011. 

8. Lecturer (2 hrs, with the title of “Antiviral Chemotherapy”) in Advanced Virology course 

for Ph.D. students of Bacteriology, Pasteur institute of Iran, November 2010 to present. 

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9. Lecturer (2 hrs, with the title of “Probiotics and Prebiotics”) in Physiology of 

Microorganisms course for M.Sc. students of Microbiology, Pasteur institute of Iran, 

November 2010 to present. 

10. Full lecturer and instructor in "Practical Immunology" course for B.Sc. students, Azad 

Univ., Karaj Branch, Karaj, 2008 to 2011. 

11. Lecturer (4 hrs) in "Control of Gene Expression" course for M.Sc. students, Azad Univ., 

Science & Research Branch, Tehran, 2009 to present. 

12. Lecturer (2 hrs, with the title of “HCV Vaccines”) in Vaccinology course for Ph.D. 

students of Medical Biotechnology, Pasteur institute of Iran, October 2010. 

13. Lecturer and instructor in "Immuno-informatics" course for Ph.D. students, Shahid 

Chamran Univ. of Medical Sciences, Ahwaz, 2009. 

14. Invited speaker in workshop on “Purification of recombinant proteins”, Pasteur institute 

of Iran, 28 Feb.-2 Mar. 2011, (with financial support of Molecular Medicine Network). 

15. Invited speaker in workshop on “Expression and optimization of recombinant proteins in 

prokaryotic system”, Pasteur institute of Iran, 22-24 Feb. 2011, (with financial support of 

Molecular Medicine Network). 

16. Key speaker in two workshops on "PCR and its Applications", Pasteur institute of Iran, 

Dec. 22, 2008 and May 22, 2009. 

17. Lecturer in workshop on "Diagnosis of HIV Infection from DBS Samples" in the context 

of "National Sero-Behavioral HIV Surveillance in Prisoners Program of Iran Ministry of 

Health", Pasteur institute of Iran, Apr. 28-30, 2009, (with financial support of Ministry of 

Health). 

Academic Society Memberships: 

1. 2001-present Iranian Society for Biotechnology 

2. 2007-present Iranian Society for Immunology and Allergy 

3. 2011-present International Society for Infectious Diseases 

Language Skills: 

1. Fluent in writing, reading and speaking in English (General IELTS average score: 7.0; W 

7.0, R 7.0, L 7.0, S 7.5) 

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2. Elementary in French 

Computer Knowledge: 

1. Windows-based programs 

2. Internet tools, PubMed and Blast search 

3. MS-Office package (PowerPoint, Word, Excel) 

4. Statistical programs (GraphPad Prism) 

5. Image analysis programs (Photoshop, ImageJ) 

6. Bioinformatics Programs (CLC MainWorkbench, GeneRunner, Clone Manager) 

7. Immunoinformatics Programs (RankPep, PaProc, NetChop, Bimas, Syfpethi, MIF 

antigenic peptide prediction tool) 

8. Sequence alignment and phylogenetic tree analysis (MEGA4 software) 

PUBLICATIONS & PRESENTATIONS 

I) Peer-Reviewed Papers in International Journals: 

1- Baghbani-arani, F., Roohvand, F., Aghasadeghi, M.R., Eidi, A., Amini, S., Motevalli, F., 

Sadat, S.M., Memarnejadian, A., Khalili, G. Expression and characterization of E.coliderived 

hepatitis C virus ARFP/F protein. Mol Biol (Mosk) 2012, 46 (2): 1-9 (In press). 

2- Sabet, R., Behjati, M., Vahabpour, R., Memarnejadian, A., Rostami, M., Fassihi, A., 

Aghasadeghi, M.R., Saghaie, L., Miri, R. Iron chelation afforded cardioprotection against 

H 2 O 2 -induced H9C2 cell injury: Application of novel 3-hydroxy pyridine-4-one 

derivatives. International Journal of Cardiology 2012 Jan. 5 (In press). 

3- Aghasadeghi, M.R., Sharifat Salmani, A., Sadat, S.M., Javadi, F., Memarnejadian, A., 

Vahabpour, R., Zabihollahi, R., Moshiri, A., Siadat, S.D. Application of outer membrane 

vesicle of Neisseria meningitides serogroup B as a new adjuvant to induce strongly Th1- 

oriented responses against HIV-1. Current HIV Research 2011, 9 (8): 630-5. 

4- Arabi, R., Roohvand, F., Norouzian, D., Sardari, S., Aghasadeghi, M.R., Khanahmad, H., 

Memarnejadian, A., Motevalli, F. A comparative study on the activity and antigenicity 

of truncated and full-length forms of streptokinase. Polish Journal of Microbiology 

2011, 60 (3): 243-51. 

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5- Dehghani, F., Haji molla hoseini, M., Memarnejadian, A., Yeganeh, F., Mokarram 

Rezaie, A., Khaze, V., Sattari, M., Darbandi Timjani, H., Labibi, F., Mosaffa, N. 

Immunomodulatory activities of chitin microparticles on Leishmania major-infected 

murine macrophages. Archives of Medical Research 2011, 42(7): 572-6. 

6- Aghasadeghi, M.R., Bahramali, G., Sadat, S.M., Farahani, A., Mohraz, M., Davar Siadat, 

S., Mostafavi, E., Memarnejadian, A., Ardestani, M.S., Vahabpour, R., Saraji, A.A., 

Delbaz, S.A. Detection of hepatitis B virus variants in HBV monoinfected and HBV/HIV 

coinfected Iranian patients under lamivudine treatment. Current HIV Research 2011, 9 

(4): 263-9. 

7- Sadat, S.M., Zabihollahi, R, Aghasadeghi, M.R., Vahabpour, R, Siadat, S.D., 

Memarnejadian, A., Azadmanesh, K., Parivar, K. Application of SCR priming VLP 

boosting as a novel vaccination strategy against HIV-1. Current HIV Research 2011, 9 

(3): 140-7. 

8- Zabihollahi, R., Sadat, S.M., Vahabpour, R., Aghasadeghi, M.R., Memarnejadian, A., 

Ghazanfari, T., Salehi, M., Rezaei, A., Azadmanesh, K. Development of single-cycle 

replicable human immunodeficiency virus 1 mutants. Acta Virologica 2011, 55 (1): 15- 

22. 

9- Tebianian, M., Hoseini, A.Z., Ebrahimi, S.M., Memarnejadian, A., Mokarram, A.R., 

Mahdavi, M., Sohrabi, N., Taghizadeh, M. Cloning, expression, and immunogenicity of 

novel fusion protein of Mycobacterium tuberculosis based on ESAT-6 and truncated C- 

terminal fragment of HSP70. Biologicals 2011, 39 (3): 143-8. 

10- Samadi-Foroushani, M., Vahabpour, R., Memarnejadian, A., Namdar, A., Khamisabadi, 

M., Sadat, S.M., Asgarian-Omran, H., Azadmanesh, K., Kokhaei, P., Aghasadeghi, M.R., 

Hadjati, J. Immune responses regulation following antitumor dendritic cell-based 

prophylactic, concurrent, and therapeutic vaccination. Medical Oncology 2011, 28: S660- 

6. 

11- Ebrahimi, S.M., Paykari, H., Memarnejadian, A. Molecular characterization of HAO3, 

the homologue of the Bm86 tick vaccine antigen from the Iranian isolate of Hyalloma 

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anatolicum anatolicum. Experimental Parasitology 2010, Jun., 18 

(DOI:10.1016/j.exppara.2010.06.020). 

12- Memarnejadian, A., Roohvand, F. Fusion of HBsAg and prime/boosting augment Th1 

and CTL responses to HCV polytope DNA vaccine. Cellular Immunology 2010, 261(2): 

93-98. 

13- Sadat S.M., Zabihollahi R., Vahabpour R., Azadmanesh, K., Javadi, F., Siadat, SD., 

Memarnejadian, A., Parivar, K., Khanahmad Shahreza, H., Arabi Mianroodi, A., 

Hekmat, S., Aghasadeghi, MR. Designing and biological evaluation of single-cycle 

replicable HIV-1 system as a potential vaccine strategy. Clinical Microbiology and 

Infection 2010; 16(2); S334. Vienna, Austria. 

14- Ebrahimi, S.M., Tebianian, M., Toghyani, H., Memarnejadian, A., Attaran, H.R. 

Cloning, expression and purification of the influenza A (H9N2) virus M2e antigen and 

truncated Mycobacterium tuberculosis HSP70 as a fusion protein in Pichia pastoris. 

Protein Expression and Purification 2010 Mar., 70(1): 7-12. 

15- Arashkia, A., Roohvand, F., Memarnejadian, A., Rafati, S. Construction of HCVpolytope 

vaccine candidates harboring immune-enhancer sequences and primary 

evaluation of their immunogenicity in BALB/c mice. Virus Genes 2010 Feb. 40(1): 44- 

52. 

16- Memarnejadian, A., Roohvand, F., Arashkia, A., Rafati, S., Shokrgozar, M.A. Polytope 

DNA vaccine development against hepatitis C virus: A streamlined approach from in 

silico design to in vitro and primary in vivo analyses in BALB/c mice. Protein and 

Peptide Letters 2009, 16 (7): 842-50. 

II) Peer-Reviewed Papers in National Journals (in Persian language with English 

abstract): 

1- Pouriayevali, M.H., Memarnejadian, A., Sadat, S.M., Zavvar, M., Siadat, S.D., 

Hartoonian, C., Aghasadeghi, M.R. Designing and construction of bicistronic plasmid 

pIRES-Igk/mIL18/Fc: potential implications for vaccine investigations. Modarres 

Journal of Medical Sciences (Pathobiology) 2011, 14 (2): 13-23. 

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2- Arabi, R., Roohvand, F., Norouzian, D., Aghasadeghi, M.R., Memarnejadian, A., 

Khanahmad, H., Sadat, S.M., Motevalli, F. Cloning and expression of truncated and 

intact streptokinase molecules in E.coli and evaluation of their biological activities. 

Journal of Agricultural Biotechnology 2010, 2 (1): 55-67. 

3- Arashkia, A., Roohvand, F., Memarnejadian, A., Alizadeh, S., Motavali, F., 

Aghasadeghi, M.R. Immunoinformatics modeling, construction of DNA plasmids, 

inserted with hepatitis C virus CTL epitopes and their preliminary immunological 

analysis. Iranian Medical Microbiology 2010, 4 (2): 34-44. 

4- Monzavi, N., Aghasadeghi, M.R., Arabi, R., Memarnejadian, A., Sadat, S.M., 

Khanahmad, H., Ebrahimi, M., Roohvand, F. Design, cloning, expression and evaluation 

of cysteine-substitutes of intact and truncated molecules of streptokinase. Physiology and 

Pharmacology 2010, 14 (1): 56-65. 

5- Memarnejadian, A., Roohvand, F., Motevalli, F., Aghasadeghi, M.R. Experimental 

polytope DNA vaccine containing HBsAg gene for the induction of protective immunity 

against hepatitis C. Feiz, Journal of Kashan University of Medical Sciences 2009, 13 (3): 

161-73. 

6- Memarnejadian, A., Roohvand, F., Arashkia, A., Berjisian, F., Aghasadeghi, M.R. 

Designing, construction and immunogenic evaluation of polytope DNA constructs by the 

application of hepatitis C virus immunodominant epitopes in BALB/c mice. Yakhteh 

Medical Journal 2009, 11 (2): 122-33. 

7- Monzavi, N., Arabi, R., Aghasadeghi, M.R., Memarnejadian, A., Roohvand, F. 

Streptokinase: The drug of choice for thrombolytic therapy in developing countries. 

Laboratory Diagnosis (ISSN 1561-6363, www.tashkhis.com) 2009, 59: 61-68. 

8- Memarnejadian, A., Forouzandeh-Moghadam, M., Sadat, SM. and Roohvand, F. 

Cloning and evaluation of the expression for streptokinase gene with a 6xHis label in E. 

coli. Daneshvar Medicine 2007, 69: 62-8. 

9- Memarnejadian, A. and Berjisian, F.: An introduction to Telomerase and its diagnostictherapeutic 

applications. Laboratory Diagnosis (ISSN 1561-6363, www.tashkhis.com) 

2004, 29: 18-24. 

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10- Memarnejadian A., Forouzandeh moghadam M., Amini S. and Roohvand F.: 

Purification and characterization of active, N-terminally His-tagged recombinant 

streptokinase, cloned and expressed in a highly optimized condition in E. coli. Physiology 

and Pharmacology Suppl. 2003, (Spring & Summer), 21-6. 

11- Memarnejadian, A., Andalibi, S. and Roohvand, F.: Diagnosis of anti-streptokinase 

antibodies in the serum of patients and its importance for the management of 

thrombolytic therapy. Laboratory Diagnosis (ISSN 1561-6363, www.tashkhis.com) 2002, 

22: 44-51. 

III) Presentations in International Meetings: 

1- Hekmat, S., Soezi, M., Memarnejadian, A., Aghasadeghi, M.R., Sadat, S.M., Siadat, 

S.D., Rahimi, P. Designing and construction of recombinant pIPI-GFP plasmid 

expressing HIV-1 virions as a novel system for HIV-1 replication assay. Microbiotech 

11, December 2011, Braga, Portugal (Poster presentation). 

2- Baghbani-Arani, F., Roohvand, F., Aghasadeghi, M.R., Amini, S., Motevalli, F., Sadat, 

S.M., Memarnejadian, A. E. coli-generated recombinant HCV core+1 (ARFP) protein: 

a potentially valuable diagnostic and immunotherapeutic tool. 14 th Annual Meeting of the 

European Society of Clinical Virology (ESCV), September 2011, Maderia, Portugal 

(Poster presentation). 

3- Abbasi, G., Motevalli, F., Memarnejadian, A., Noorbazargan, H., Roohvand, F. An 

optimized system for the prokaryotic expression/purification of HCV ARFP/F truncated 

proteins. 1 st International and 12 th Iranian Congress of Microbiology, May 2011, 

Kermanshah, Iran (Poster presentation). 

4- Memarnejadian, A., Sadat, S.M, Aghasadeghi, M.R., Motevalli, F., Bahramali, G., 

Roohvand, F. Studies on immune-dominancy of HCV-CTL epitopes bt peptide 

immunization in BALB/c mice. 10 th International Congress of Immunology and Allergy 

of Iran, May 2010, Tehran, Iran (Oral presentation). 

5- Yazdanian, M., Roohvand, F., Memarnejadian A., Aghasadeghi, M.R., Siadat, S.D., 

Sadat Shandiz, S.A., Sohrabi, T., Khanahmad, H. Construction of recombinant BCG 

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harboring hepatitis C virus (HCV) truncated core and enhanced thymidine kinase genes 

as HCV candidate vaccines. 10 th International Congress of Immunology and Allergy of 

Iran, May 2010, Tehran, Iran (Poster presentation). 

6- Baghbani Arani, F., Aghasadeghi, M.R., Sadat, M., Motevalli, F., Memarnejadian, A., 

Roohvand, F. Comparison of different adjuvants for the induction of humoral response 

against HCV ARFP/F synthetic peptides in BALB/c mice. 10 th International Congress of 

Immunology and Allergy of Iran, May 2010, Tehran, Iran (Poster presentation). 

7- Sadat, M., Baghbani Arani, F., Aghasadeghi, M.R., Motevalli, F., Memarnejadian, A., 

Kadkhodayan, S., Alizadeh Sabuni, Sh., Roohvand, F. Studying the capacity of HCV 

ARFP/F peptides to induce humoral immune responses in BALB/c mice. 10 th 

International Congress of Immunology and Allergy of Iran, May 2010, Tehran, Iran 


8- Hashemi, A., Memarnejadian, A., Motevalli, F., Edalar, R., Vahabpour, R., 

Noorbazargan, H., Roohvand, F., Ghahremani, M.H. Differential expression of interferon 

regulatory factor 1 by native and truncated HCV core proteins. 10 th International 

Congress of Immunology and Allergy of Iran, May 2010, Tehran, Iran (Poster 

presentation). 

9- Tebianian, M., Zavaran Hoseini, A., Ebrahimi, S.M., Rezaei Mokarram, A., 

Memarnejadian, A., Taghizadeh, M., Sohrabi, N. Cloning, expression and purification 

of Mycobacterium tuberculosis ESAT-6 and HSP70 (359-610) as a tuberculosis vaccine 

candidate in a prokaryotic system. 10 th International Congress of Immunology and 

Allergy of Iran, May 2010, Tehran, Iran (Poster presentation). 

10- Arabi, R., Roohvand, F., Sardari, S., Memarnejadian, A., Aghasadeghi, M.R., 

Noroozian, D., Khanahmad, H. Modeling and genetic engineering of streptokinase 

structure to produce a fibrin-dependent protein with lower antigenicity. 16 th National and 

4 th International Conference of Biology, Sep. 2010, Mashhad, Iran (Poster presentation). 

11- Baghbani Arani, F., Motevalli, F., Aghasadeghi, M.R., Sadat, S.M., Memarnejadian, A., 

Eidi, A., Roohvand, F. Over-expression, purification and characterization of HCV core+1 

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protein. 16 th National and 4 th International Conference of Biology, Sep. 2010, Mashhad, 

Iran (Poster presentation). 

12- Abbasi, G., Motevalli, F., Memarnejadian, A., Noorbazargan, H., Roohvand, F. Cloning 

and expression of 86-161 and 43-143 fragments of HCV ARFP/Fprotein in E .coli. 16 th 

National and 4 th International Conference of Biology, Sep. 2010, Mashhad, Iran (Oral 


13- Tebianian, M., Zavaran Hoseini, A., Ebrahimi, S., Memarnejadian, A., Rezaei 

Mokarram, A., Taghizadeh, M., Sohrabi, N. Construction and immunogenicity of a novel 

Mycobacterium tuberculosis recombinant fusion protein based on ESAT-6 and 

HSP70 (359-610) . 14 th International Congress of Immunology, Aug, 2010, Kobe, Japan 


14- Samadi, M., Vahabpour, R., Khamisabadi, M., Namdar, A., Asgarian, H., Amini, A., 

Azadmanesh, K., Memarnejad, A., Sadat, M., Aghasadeghi, M., Hajati, J. Evaluation of 

regulatory responses after vaccination with activated dendritic cell vaccine in mice. 14 th 

International Congress of Immunology, Aug, 2010, Kobe, Japan (Poster presentation). 

15- Motevalli, F., Sadat, S.M., Memarnejadian, A., Bahramali, G., Alizadeh, S., 

Aghasadeghi, M.R., Roohvand, F. Immune-dominancy of HCV CTL epitopes on each 

other is less profound in immunization by a single polytopic-peptide vaccine than mixture 

of the minimal-CD8+ peptides. 17 th International Meeting on Hepatitis C Virus and 

Related Viruses, Sept. 2010, Yokohama, Japan (Poster presentation). 

16- Keramati, M., Aslani, M., Aghasadeghi, M., Motevalli, F., Memarnejadian, A., Mirzaie, 

A., Roohvand, F. Cloning, expression and characterization of Streptokinase gene from a 

group G streptococcus in E. coli. 11 th Iranian Microbiology Congress and 1 st East 

Mediterranean Microbiology Congress, May 2010, Langrood, Iran (Poster presentation). 

17- Shandiz, S.A., Yazdanian, M., Aghasadeghi, M.R., Khanahmad, H., Memarnejadian, 

A., Motevalli, F., Sohrabi, T., Roohvand, F. Construction of recombinant BCG HCV 

polytope candidate vaccine. 11 th Iranian Microbiology Congress and 1 st East 


15


18- Roohvand, F., Motevali, F., Ghahramani, M.H., Noorbazargan, H., Memarnejadian, A., 

Hashemi, A., Aghasadeghi, M.R. Expression studies of native and truncated HCV core 

proteins in mammalian cells. 11 th Iranian Microbiology Congress and 1 st East 


19- Noorbazargan, H., Hashemi, A., Motevalli, F., Memarnejadian, A., Aghasadeghi, M.R., 

Roohvand, F. Expression of truncated HCV core+1 protein in hepatic mammalian cells. 

11 th Iranian Microbiology Congress and 1 st East Mediterranean Microbiology Congress, 

May 2010, Langrood, Iran (Poster presentation). 

20- Aghasadeghi, MR., Farahani far, A., Bahramali, G., Sadat, SM., Memarnejadian, A., 

Siadat, SD., Mohraz, M., Foroughi, M., Javadi, F., Roohvand, F., Vahabpour, R., 

Pouriayevali, MS. Prevalence of HBV Lamivudine-resistance mutations in a cohort of 

Iranian HBV-infected patients with and without HIV co-infection. 20th European 

Congress of Clinical Microbiology and Infectious Diseases, April 2010, Vienna, Austria 


21- Sadat, SM., Zabihollahi, R., Vahabpour, R., Azadmanesh, K., Javadi, F., Siadat, SD., 

Memarnejadian, A., Parivar, K., Khanahmad Shahreza, H., Arabi Mianroodi, A., 

Hekmat, S., Aghasadeghi, MR. Designing and biological evaluation of single-cycle 

replicable HIV-1 system as a potential vaccine strategy. 20th European Congress of 

Clinical Microbiology and Infectious Diseases, April 2010, Vienna, Austria (Poster 


22- Farahani far, A., Aghasadeghi, MR., Bahramali, G., Sadat, SM., Siadat, SD., Javadi, F., 

Roohvand, F., Amini, S., Hekmat, S., Memarnejadian, A., Motevalli, F., Jolaei, M.: 

HBV genotypes and subtypes in Iranian HBV infected patients with and without HIV 

infection. 3 rd Eurasia Congress of Infectious Diseases, October 2009, Baku, Azerbaijan 


23- Roohvand, F., Memarnejadian, A., Arashkia, A., Aghasadeghi, MR., Sadat, M., 

Budkowska, A., Combadière, B.: French-Iranian collaborative studies on HCV vaccines 

(CD8-polytopic DNA constructs and protein based immunogens as therapeutic and 

prophylactic vaccine candidates). Research on Infectious Diseases: A Global Challenge, 

June 2008, Institut Pasteur, Paris, France. (Oral presentation). 

16


24- Roohvand, F., Memarnejadian, A., Arashkia, A.: Construction and Evaluation of CD8- 

polytope DNA constructs based on immunodominant and sub-dominant HCV epitopes. 

1 st Vaccine Congress, Dec. 2007, Amsterdam, the Netherlands. (Oral presentation). 

25- Memarnejadian, A., Arashkia, A., Rafati, S., Shokrgozar, M., Roohvand, F.: 

Engineering, construction and immunological evaluation of polytope DNA constructs 

based on immunodominant HCV epitopes. 1 st International Congress on Health 

Genomics and Biotechnology, Nov. 2007, Tehran, Iran. (Oral presentation). 

26- Arashkia, A., Memarnejadian, A., Rafati, S., Roohvand, F.: An study on the effect of 

ER signal sequence and a Pan-DR Epitope (PADRE) on the immunogenicity of a CTLbased 

HCV epitope. 1 st International Congress on Health Genomics and Biotechnology, 

Nov. 2007, Tehran, Iran. (Oral presentation). 

27- Arashkia, A., Memarnejadian, A., Roohvand, F.: Construction and Evaluation of CD8- 

polytope DNA constructs based on immunodominant and sub-dominant HCV epitopes. 

14th International Symposium on Hepatitis C Virus and Related Viruses, Sept. 2007, 

Glasgow, Scotland. (Poster presentation). 

IV) Presentations in National Meetings: 

1- Soezi, M., Aghasadeghi, M.R., Memarnejadian, A., Aminzadeh, S., Amini, S., Sadat, 

S.M., Hekmat, S. Construction of single-cycle replicable HIV-1 virions expressing GFP 

reporter protein. 5 th Iranian Congress of Clinical Microbiology, November 2011, Tehran, 

Iran (Oral presentation). 

2- Abbasi, G., Motevalli, F., Memarnejadian, A., Roohvand, F. Cloning, expression and 

purification of recombinant fragments from HCV core+1 protein for their 

characterization. 7 th National Biotechnology Congress of Iran, September 2011, Tehran, 

Iran (Poster presentation). 

3- Baghbani-Arani, F., Roohvand, F., Aghasadeghi, M.R., Eidi, A., Amini, S., Motevalli, F., 

Sadat, S.M., Memarnejadian, A. Production and immunological characterization of 

Hepatitis C Virus F Protein. 7th National Biotechnology Congress of Iran, September 

2011, Tehran, Iran (Poster presentation). 

17


4- Hashemi, A., Roohvand, F., Ghahremani, M.H., Aghasadeghi, M.R., Motevalli, F., 

Vahabpour, R., Memarnejadian, A., Noorbazargan, H. Optimization and Comparison of 

chemical-based gene delivery methods to electroporation for use in Huh-7 and Vero cells. 

7th National Biotechnology Congress of Iran, September 2011, Tehran, Iran (Poster 


5- Keramati, M., Roohvand, F., Aslani, M.M., Aghasadeghi, M.R., Khatami, S., Motevalli, 

F., Memarnejadian, A., Mirzaie, A. Characterization, isolation and expression of 

streptokinase from clinical strain streptococci group C in comparison with reference 

strain. 7th National Biotechnology Congress of Iran, September 2011, Tehran, Iran 


6- Noorbazargan, H., Hashemi, A., Aghasadeghi, M.R., Motevalli, F., Sadat, S.M., 

Memarnejadian, A., Asmar, M., Roohvand, F., Construction of vector expressing 

ARFP/F /Core+1 protein in eukaryotic cells. Regional Meeting on Recent Findings of 

Molecular Microbiology, 2010, Lahijan, Iran (Oral presentation). 

7- Keramati, M., Memarnejadian, A., Aslani, M.M., Mirzaie, A., Roohvand, F. 

Comparison of three refolding methods for purified recombinant streptokinase. 3 rd 

Iranian Proteomics Congress, May 2010, Pasteur institute of Iran, Tehran, Iran (Poster 


8- Shandiz, S.A., Yazdanian, M., Aghasadeghi, M.R., Khanahmad, H., Memarnejadian, 

A., Motevalli, F., Sohrabi, T., Roohvand, F. Design and Construction of a polytope HCV 

vaccine candidate plasmid for expression in BCG. 11 th Iranian Congress of Genetics, 

May 2010, Tehran, Iran (Poster presentation). 

9- Noorbazargan, H., Hashemi, A., Motevalli, F., Memarnejadian, A., Aghasadeghi, M.R., 

Asmar, M., Roohvand, F. Expression of HCV ARFP/DF protein in hepatic cells. 11 th 

Iranian Congress of Genetics, May 2010, Tehran, Iran (Poster presentation). 

10- Hashemi, A., Motevalli, F., Memarnejadian, A., Aghasadeghi, M.R., Noorbazargan, 

M.H., Ghahramani, M.H., Roohvand, F. Expression of domains 1 and 2 of HCV core 

protein in Huh7 eukaryotic cell line. 11 th Iranian Congress of Genetics, May 2010, 

Tehran, Iran (Poster presentation). 

18


11- Pouriaye vali, M.H., Memarnejadian, A., Sadat, S.M., Siadat, S.D., Roohvand, F., 

Hekmat, S., Amini, S., Hartoonian, C., Aghasadeghi, M.R. Designing and construction of 

bicistronic plasmid capable of simultaneous expression of antigen and IL18/Ig adjuvant. 

11 th Iranian Congress of Genetics, May 2010, Tehran, Iran (Poster presentation). 

12- Arabi, R., Norouzian, D., Aghasadeghi, MR., Khanahmad, H., Memarnejadian, A., 

Sadat, SM., Roohvand, F.: Cloning and expression of truncated and intact molecules of 

streptokinase in E. coli and evaluation of their biological activities. 6th National 

Biotechnology Congress of Iran, August 2009, Milad Tower Conference Hall, Tehran, 


13- Monzavi, N., Arabi, R., Aghasadeghi, MR., Memarnejadian, A., Sadat, SM., 

Khanahmad, H., Hekmat, S., Roohvand, F.: Design, cloning, expression and evaluation 

of cysteine analogs of truncated and intact molecules of streptokinase. 6th National 

Biotechnology Congress of Iran, August 2009, Milad Tower Conference Hall, Tehran, 


14- Memarnejadian, A., Roohvand, F., Bonduelle, O., Ghadiri, A., Combadière, B.: 

Dependency of the T cell responses on the linear arrangement of CTL epitopes 

incorporated into HCV multiepitope vaccines. 5th Iranian Congress of Virology & 1st 

Congress of Vaccine, May 2009, Razi Vaccine and Serum Research Institute, Tehran, 

Iran. (Oral presentation). 

15- Arashkia, A., Roohvand, F., Memarnejadian, A., Bonduelle, O., Ghadiri, A., 

Combadière, B.: Evaluation of two HCV subdominant epitope immunogenicity in 

transgenic mice and the effect of helper factors. 5th Iranian Congress of Virology & 1st 

Congress of Vaccine, May 2009, Razi Vaccine and Serum Research Institute, Tehran, 

Iran. (Poster presentation). 

16- Tebianian, M., Zavaran Hoseini, A., Rezaei Mokarram, A., Ebrahimi, S.M., Taghizadeh, 

M., Memarnejadian, A.: Cloning and expression of Mycobacterium tuberculosis ESAT- 

6 in prokaryotic system. 5th Iranian Congress of Virology & 1st Congress of Vaccine, 

May 2009, Razi Vaccine and Serum Research Institute, Tehran, Iran. (Poster 


19


17- Roohvand, F., Memarnejadian, A., Arashkia, A., Aghasadeghi, M., Amini, S., Andalibi 

Mahmoudabadi, S., Budkowska, A.: Exploiting the His-tag based, one-step purification 

systems for expression of interested genes, A comparative study on productivity / yield 

for genes of HCV-core, HBe Ag and streptokinase (SK). 8 th Iranian Genetics Congress. 

May 2003, Tehran, Iran. (Oral presentation). 

18- Memarnejadian A., Forouzandeh moghadam M., Amini S. and Roohvand F.: 

Purification and characterization of active, N-terminally His-tagged recombinant 

streptokinase, cloned and expressed in a highly optimized condition in E. coli. Physiology 

and Pharmacology Congress, May 2003, Tarbiat Modarres Univ., Tehran, Iran (Oral 


19- Memarnejadian, A., Forouzandeh, M., Amini, S., Roohvand, F.: Cloning and 

optimization of condition for expression of the streptokinase gene in a His-tag based one 

step purification system in E. coli. 1 st National Congress of Molecular Cell Biology, Feb. 

2003, Shahid Chamran Univ., Ahwaz, Iran. (Oral presentation). 

REFERENCES 

1- Dr. FARZIN ROOHVAND, Ph.D. 

Associate Prof. and Senior Researcher 

Hepatitis & AIDS Dept., Pasteur institute of Iran, Tehran 1316943551, Iran 

Phone / FAX: +98.21.66969291 

Email: farzin.roohvand@gmail.com and rfarzin@pasteur.ac.ir 

* I have been acquainted with Dr. Roohvand for around 10 years. He has supervised both my 

M.Sc. and Ph.D. theses. 

2- Dr. BEHAZINE COMBADIÈRE, Ph.D. 

Research director DR2 INSERM 

Coordinator of the European Project 

FP7-EU: CUT’HIVAC; Cutaneous and mucosal HIV Vaccine 

FRM "Immunotherapy team": Skin's route of vaccination 

20


Laboratory of Immunity and Infection - INSERM U945 

Université Pierre et Marie Curie, 91 bld de l'hopital - 75013 Paris 

Phone: +33.1.40779888, FAX: +33.1.40779734 

Email: behazine.combadiere@upmc.fr 

* Dr. Combadière co-supervised of my Ph.D. thesis and accepted me at her Lab. in 2008 to 

pursue pats of my thesis during six months. 

3- Prof. WATARU SUGIURA, M.D., Ph.D. 

Group Leader 

Laboratory of Therapeutic Research and Clinical Science, AIDS Research Center 

National Institute of Infectious Diseases 

Tokyo 2080011, Japan 

Department Chair, 

Department of Infection and Immunity 

Center for AIDS Research, Education and Support (CARES) 

National hospital organization, Nagoya Medical Center 

4-1-1 Sannomaru, Naka-Ku, Nagoya, Japan 

Phone: +81.52.951.1111, FAX: +81.52.963.3970 

Email: wsugiura@nnh.hosp.go.jp and taru1600@aol.jp 

* Prof. Sugiura accepted me at his Lab. for two months in 2010 and supervised my training 

on HIV molecular epidemiology and drug-resistance detection. 

4- Prof. SIMA RAFATI, Ph.D. 

Group Leader, 

Laboratory of Molecular Immunology and Vaccine Research 

Immunology Dept., Pasteur institute of Iran, Tehran 1316943551, Iran 

Phone / FAX: +98.21.66496560 

Email: sima-rafatisy@pasteur.ac.ir and s_rafati@yahoo.com 

* I have been acquainted with Prof. Rafati as her student during my Ph.D. immunology 

course, as well as her capacity as the advisor of my Ph.D. thesis. 

21

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