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Optix<br />
Illumination<br />
Module<br />
The Optix module is a patented epiillumination<br />
option for ULTima Pro; for<br />
the excitation of fluorophores within<br />
the VIS/NIR range. When Optix is used<br />
for ethidium bromide stained gels, it<br />
offers the following advantages:<br />
DNA remains intact for applications<br />
downstream.<br />
Lower background means higher<br />
detection limits.<br />
Selection of excitation wavelength is<br />
fully computer controlled and, in<br />
combination with the automatic<br />
emission filter changer, true multi<br />
fluorophore capability is achieved.<br />
Multiple images with different<br />
excitation/emission filter sets allow<br />
the detection of multiple<br />
fluorophores within the same<br />
sample. The Optix can<br />
accommodate a maximum of five<br />
different excitation filter sets. The<br />
Optix module can be added to all<br />
models of the ULTima line.<br />
Applications that are possible with<br />
the addition of the Optix module<br />
include: CyDye imaging, array<br />
detection, in vivo GFP detection and<br />
GFP tissue and organ screening.<br />
Pseudo-color image<br />
of macro array,<br />
labeled with Cy5.5<br />
GFP detection<br />
in leaves.<br />
TotalLab <br />
Analysis<br />
Software<br />
Select the software that best meets your needs by your area of work below:<br />
Molecular Weight Calibration<br />
The TL 100 and TL 120 versions both enable precise molecular weight determination of<br />
band material from a variety of 1D gels. TL 120 contains tools which allow increased<br />
control over calibration for particularly distorted 1D gel images.<br />
Quantitation and Normalization of Band Material<br />
The TL 100 and TL 120 software solutions both contain a complete range of tools to<br />
quantify band material and for the calculation of normalized band volumes. For in-depth<br />
quantitative studies, the advanced features in TL 120 offer a higher degree of flexibility<br />
and facilitate accurate quantitation of bands on more problematic 1D gel images.<br />
Lane Relationship Studies<br />
The TL 120 software has a powerful band matching facility which is flexible yet easy to<br />
use. There are tools available to visualize the results of matching and identify similarities<br />
within a single gel. When you have performed intra gel matching, lanes can be classified<br />
and identified.<br />
TL 100<br />
Entry level software<br />
for fast, consistent<br />
analysis.<br />
TL 120<br />
For an in-depth and<br />
accurate automatic<br />
analysis of 1D gels<br />
and band pattern<br />
matching studies.<br />
The TotalLab analysis software provides a complete set of tools for<br />
fast, accurate and easy analysis of images from the ULTima for<br />
molecular weight determination, sample quantitation, arrays, dot<br />
blot/slot blot analysis and colony counting.<br />
Summary of features in the<br />
TotalLab range: TL 100 TL 120<br />
Automatic detection of lanes and bands<br />
Automatic background subtraction<br />
Lane templates<br />
Image manipulation tools<br />
Analysis protocols for batch processing<br />
Molecular weight calibration<br />
Quantity calibration<br />
Normalization<br />
Profile deconvolution<br />
Rf calibration<br />
Band picking<br />
Band pattern matching - single gel<br />
Dendrogram - single gel<br />
Reports<br />
Array, dot / slot blot analysis<br />
Colony counting<br />
A toolbox for general image analysis
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