*ULTima Brochure - Hoefer®, Inc

ULTima
The ULTimate in
Gel Documentation
and Imaging

The ULTima imaging platform is the fastest, most straightforward and
readily upgradable unit available, incorporating the latest advances in
electronics and optics. The ULTima can economically handle everything from
the most routine to the most demanding applications in proteomics and
genomics, which have until now required expensive laser scanning systems.
Full line of upgradable instruments (ULTima10si, 16si, 16si Plus and Pro)
allows you to upgrade to higher specifications as research needs change.
High resolution gel documentation and
imaging with exceptional sensitivity and
ease of use.
Patented image positioning tool aligns the sample from the computer
and permits imaging of a selected part of a large gel or blot.
Patented slant correction tool corrects the orientation of the sample from
the computer.
Mega-pixel CCD cameras with 10 bit and 16 bit arrays provide very high
resolution images.
Large 21 x 26 cm imaging area allows documentation of large samples
like 2D gels.
Superior dynamic range with the 16si and Pro models with 65536 gray
levels allows precise quantification of samples and shortens exposure
times for chemiluminescent images.
Compact design saves precious lab space.
ULTima Pro permits true multi-fluorophore imaging when used with the
Optix illumination module.
Image addition tools permit kinetic studies.
All models of ULTima permit easy access to robots.
All models of the ULTima permit GLP validation.
All models can be upgraded as research needs grow.
ULTima
Imaging
Platform
Broadest Range
of Applications.
Fluorescent and Calorimetric Imaging
with a wide array of dyes.
Absorbance/Visible Imaging.
Culture and Microplate Based Assays.
Large Sized 1D and 2D Gels for
proteomics applications.
Chemiluminescent imaging on ULTima
Pro cooled CCD system.
Multi-fluorophore imaging with ULTima
Pro and Optix illumination module.

Unique Patented Image Positioning and Slant Correction
Positioning tools on ULTima AQ software help position gels or other samples
from the computer. The positioning is easy, accurate and fast with minimum
handling of fragile gels or other samples. Every user of an imaging system has
experienced the problem that the gel is never in the right place initially. With
most commercially available systems the user has to open the drawer or door
and manually handle the gel for the best visualization. The ULTima, however,
is the only imaging system with patented optics for image positioning without
loss of optical resolution. This also ensures that lens adjustments are not
required. The image is positioned with the positioning keys without having to
open the cabinet or adjust a fragile gel.
Slant correction tools permit optical rotation
of the sample for best ‘straight’ visualization
without touching the gel. Slant correction
is a unique feature that allows the user to
turn the image optically ± 5°. In this way
the sample can be positioned so the analysis
software is better able to perform lane
detection.
Flatfielding
ULTima imaging systems allow
accurate quantitative measurements
of samples with a unique feature
called flatfielding. Flatfielding is done
in two easy steps after selecting the
flatfielding option on the ULTima AQ
software. Flatfielding removes
artifacts resulting from small
variations in the CCD and uneven
background presenting a true image
of sample intensities. The Orange
conversion plate is used to create a
flatfield image and the ULTima AQ
software uses this flatfield image to
“clean up” any variations to present
raw image data that is highly
accurate.
Image Addition
ULTima imaging systems allow users
to simultaneously capture multiple
images of the same sample at a set
interval with a unique feature called
Image Addition. The user can set the
number of images to be ‘added’ and
the time interval between
acquisitions. This feature allows the
ULTima to be used in kinetic studies.
If the image addition is used in
combination with the Auto-Save
function, all the individual images will
be stored. The added result should be
stored manually.
Templates
Templates allow the user to define the
settings for image acquisition,
including the excitation and emission
filters. The parameters are stored per
specific application.
Orange Conversion Plate
The optional orange conversion plate
allows transillumation of visible dyes
like Coomassie and silver stain on a
UV transilluminator. The conversion
plate is also required for flatfielding
the image.

How to choose
your ULTima?
There are four systems plus the optional Optix illumination module. All models
can be upgraded to higher specified versions giving you the flexibility to
purchase the model that meets your needs and budget today and upgrade it
as your needs increase. All models come with ULTima AQ control and image
acquisition software and ethidium bromide emission filter.
UV Transilluminator Orange Conversion Plate

ULTima 10si & 16si
Use ULTima 10si for basic documentation purposes. With
ULTima 16si your measurements are more sensitive and
allow more accurate quantification. Both models can be
used for imaging ethidium bromide stained DNA agarose
gels, Coomassie stained gels, SYBR Green stained gels,
SYPRO Ruby stained gels, film archiving and petri dishes.
General purpose gel documentation system.
Fluorescence ready.
ULTima 10si has a 10 bit CCD camera (1024 gray
levels) and ULTima 16si has a 16 bit CCD camera
(65536 gray levels).
Image positioning / Slant correction.
Electronic optical zoom (6X).
White light top illumination.
ULTima 16si Plus
High dynamic range general purpose gel
documentation system.
ULTima 16si Plus has a 16 bit CCD camera
(65536 gray levels).
Electronic optical iris and electronic optical focus.
ULTima 16si Plus is equipped for multi-plexed
multi-fluorophore applications.
Image positioning / Slant correction.
Electronic optical zoom (6X).
White light top illumination.
ULTima Pro
Designed with innovative 2-stage Peltier cooling of the image
sensor. Rather than attempt to cool the entire CCD chip to
a very low temperature, the ULTima Pro cools only the areas
of the chip where cooling is essential to achieve low noise
levels at long measurement times. This substantially
lengthens the lifetime of the CCD chip and the camera
system. As with ULTima 16si Plus, the ULTima Pro is equipped
with an additional computer-controlled focus and iris control.
The ULTima Pro comes with a small external power supply
that drives the Peltier cooling system.
High sensitivity chemiluminescence imaging system.
ULTima Pro has the top of the line 16 bit temperature
controlled cooled CCD camera (65536 gray levels).
Electronic optical iris and electronic optical focus.
Image positioning / Slant correction.
Electronic optical zoom (6X).
White light top illumination.
Technical Specifications
Model
Resolution (pixel)
Resolution (sec.)
Image Sensor Cooling
Camera Bit Depth
Electronic Optical Zoom
Electronic Iris Control
Electronic Focus Control
Image Position System
Slant Control
Emission Filter Positions
White Light Top Illumination
Automatic Door Lock
Contrast Control
Brightness Control
Flat Fielding
Saturation Indication
Image Addition (x)
Addition Interval (min.)
10si
Filetype: tiff (16 bit), Tif (8 bit), bmp, jpg, gif
16si
16si Plus Pro
Mains: 110/230 Voltage AC automatic detection 50-60 Hz 180 Watt max. Installation Cat. II
Outer Dimensions (W x H x D): 510 x 380 x 500 mm
Weight: 41 kg.
1312 x 1032
0.001 - 10
No
10
6 x
No
No
Yes
± 5°
1
(5 optional)
Yes
Yes
Yes
Yes
Yes
Yes
No
No
1392 x 1040
0.001 - 10
IMPORTANT: For installing and using the ULTima and ULTima AQ software the
following computer minimum specifications are required:
Computer working under Windows 2000, Windows XP-Home (SP1) or XP-Professional
(SP1) minimum 256 Mb RAM, minimum screen resolution 1024 x 768, one free USB 2.0
port (800 mA or 500 mA) one free RS-232C port (9 pin), pointing device, 1 Gb free harddisk
space.
No
16
6 x
No
No
Yes
± 5°
1
(5 optional)
Yes
Yes
Yes
Yes
Yes
Yes
1 - 25
0 - 20
1392 x 1040
0.001 - 10
No
16
6 x
Yes
Yes
Yes
± 5°
1
(5 optional)
Yes
Yes
Yes
Yes
Yes
Yes
1 - 25
0 - 20
1392 x 1040
0.001 - 3000
Yes, 6 arrays
16
6 x
Yes
Yes
Yes
± 5°
1
(5 optional)
Yes
Yes
Yes
Yes
Yes
Yes
1 - 25
0 - 20

Optix
Illumination
Module
The Optix module is a patented epiillumination
option for ULTima Pro; for
the excitation of fluorophores within
the VIS/NIR range. When Optix is used
for ethidium bromide stained gels, it
offers the following advantages:
DNA remains intact for applications
downstream.
Lower background means higher
detection limits.
Selection of excitation wavelength is
fully computer controlled and, in
combination with the automatic
emission filter changer, true multi
fluorophore capability is achieved.
Multiple images with different
excitation/emission filter sets allow
the detection of multiple
fluorophores within the same
sample. The Optix can
accommodate a maximum of five
different excitation filter sets. The
Optix module can be added to all
models of the ULTima line.
Applications that are possible with
the addition of the Optix module
include: CyDye imaging, array
detection, in vivo GFP detection and
GFP tissue and organ screening.
Pseudo-color image
of macro array,
labeled with Cy5.5
GFP detection
in leaves.
TotalLab
Analysis
Software
Select the software that best meets your needs by your area of work below:
Molecular Weight Calibration
The TL 100 and TL 120 versions both enable precise molecular weight determination of
band material from a variety of 1D gels. TL 120 contains tools which allow increased
control over calibration for particularly distorted 1D gel images.
Quantitation and Normalization of Band Material
The TL 100 and TL 120 software solutions both contain a complete range of tools to
quantify band material and for the calculation of normalized band volumes. For in-depth
quantitative studies, the advanced features in TL 120 offer a higher degree of flexibility
and facilitate accurate quantitation of bands on more problematic 1D gel images.
Lane Relationship Studies
The TL 120 software has a powerful band matching facility which is flexible yet easy to
use. There are tools available to visualize the results of matching and identify similarities
within a single gel. When you have performed intra gel matching, lanes can be classified
and identified.
TL 100
Entry level software
for fast, consistent
analysis.
TL 120
For an in-depth and
accurate automatic
analysis of 1D gels
and band pattern
matching studies.
The TotalLab analysis software provides a complete set of tools for
fast, accurate and easy analysis of images from the ULTima for
molecular weight determination, sample quantitation, arrays, dot
blot/slot blot analysis and colony counting.
Summary of features in the
TotalLab range: TL 100 TL 120
Automatic detection of lanes and bands
Automatic background subtraction
Lane templates
Image manipulation tools
Analysis protocols for batch processing
Molecular weight calibration
Quantity calibration
Normalization
Profile deconvolution
Rf calibration
Band picking
Band pattern matching - single gel
Dendrogram - single gel
Reports
Array, dot / slot blot analysis
Colony counting
A toolbox for general image analysis

TL 100 Control Center
Analysis protocols enable consistent processing of
multiple gels in TL 120.
ID Gel Image analyzed by TL 100 Calibration of a 1D gel using multiple molecular marker lanes in TL 120.

953 Indiana Street
San Francisco, CA 94107 USA
Hoefer is a Harvard Bioscience company.
www.hoeferinc.com
Toll Free: 1-800-227-4750
Local Phone: 1-415-282-2307
Customer Service:
Select 1 from the main menu
Technical Support:
Select 2 from the main menu.
Fax: 1-415-920-4494 or 1-415-821-0239
Online Ordering: www.hoeferinc.com
Email: support@hoeferinc.com
For inquiries please contact your local
Hoefer representative below:
Trademarks:
ULTima, Optix and Hoefer are trademarks of Hoefer, Inc.
TotalLab is a trademark of Non Linear Dynamics.
Windows and Windows XP are trademarks of Microsoft Corp.
SYBR Green is a trademark of Molecular Probes.
Cy3 and Cy5 are trademarks of GE Healthcare.
Coomassie is a trademark of ICI.
©2006 Hoefer, Inc. - All rights reserved.
Printed in the USA.
Ordering the ULTima
Basic System
The basic package includes the ULTima model of your choice, ULTima AQ communication suite
and Ethidium Bromide orange filter. ULTima 16si Plus and Pro models come with a filter selector.
ULTima Pro comes with a power supply for the cooling Peltier.
ULT101 ULTima 10si imaging unit
ULT161 ULTima 16si imaging unit
ULT161F ULTima 16si Plus imaging unit
ULT161C ULTima Pro cooled CCD imaging unit
Complete System
Use part numbers below to order a complete ULTima package.
The complete system package includes ULTima model of your choice, ULTima AQ communication
suite and Ethidium Bromide orange filter. UV-26 transilluminator and TL100 Total Lab analysis
software. ULTima 16si Plus and Pro models come with a filter selector. ULTima Pro comes with a
power supply for the cooling Peltier.
ULT10 ULTima 10si imaging system
ULT16 ULTima 16si imaging system
ULT16F ULTima 16si Plus imaging system
ULT16C ULTima Pro cooled CCD imaging system
Transilluminator and Analysis Software Sold Separately:
UV26 UV-26 Transilluminator with 21 x 26 cm imaging area
ULT162 TL100 TotalLab analysis software Single user license
ULT163 TL120 TotalLab analysis software Single user license
ULT215 TL100 TotalLab image analysis Network version includes 3 user license
ULT216 TL100 TotalLab image analysis Network single user license
ULT217 TL100 TotalLab image analysis additional single user license for standalone installations
Optional Accessories:
ULT164 Optix illumination module (only for ULTima Pro)
ULT165 Orange conversion plate
ULT220 Emission filter changer for 5 filters ( for ULTima 16si )
ULT312 UV transillumination cassette 312 nm. 21 x 26 cm
ULT306 UV Top light source basic holder
ULT365 UV Top light extension 365 nm
ULT302 UV Top light extension 302 nm.
ULT256 UV Top light extension 256 nm.
ULT201 Emission filter 480-550 (e.g. EBFP, ECF) for mounting on filterchanger
ULT202 Emission filter 480-550 (e.g. EBFP, ECF) for mounting on lens
ULT203 Emission filter 515-565 (e.g. SYBR Green, EGFP, EYFP) for mounting on filterchanger
ULT204 Emission filter 515-565 (e.g. SYBR Green, EGFP, EYFP) for mounting on lens
ULT205 Emission filter 570-650 (e.g. Eth. Bromide filter, Cy3) for mounting on filterchanger
ULT206 Emission filter 570-650 (e.g. Eth. Bromide filter, Cy3) for mounting on lens
ULT207 Emission filter 665-730 (e.g. Cy5) for mounting in filterchanger
ULT208 Emission filter 665-730 (e.g. Cy5) for mounting on lens
ULT210 Black Sample tray (Luminescent / Optix)
ULT211 Excitation filter set for Optix, 450 nm
ULT212 Excitation filter set for Optix, 500 nm
ULT213 Excitation filter set for Optix, 555 nm ( standard)
ULT214 Excitation filter set for Optix, 650 nm
ULT220 USB to RS232 serial adapter for computers without a serial port