Phoretix™ 2D Evolution: 2D Gel Analysis Software - PerkinElmer

Phoretix 2D Evolution2D Gel Analysis SoftwareThe analysis of 2D gels in order to determine protein spots of interest forspot excision and subsequent MS identification is historically labor intensiveand highly subjective. Traditional analysis packages lack the sensitivityto detect low abundance proteins. It is these spots that are often the proteinsof real interest.There has been a need for an analysis package that is highly sensitive, lessgel-dependent and provides a greater degree of consistency.Phoretix 2D Evolution is a new 2D analysis package that fulfills these needs.It is the ideal image analysis package for low- to medium-throughput gelbasedproteomics laboratories, and provides an entry point into theProgenesis family of products.Proven Increase in Sensitivity and SpeedFigure 1A.Standard Software815 spots detected.60 minutes total editing time.Figure 1B.Phoretix 2D Evolution1479 true spots automatically detected.5 minutes total editing time.“Phoretix 2D Evolution brings a new level of versatility andsensitivity to our proteomics work involving 2D electrophoresis.It is the undisputed 2D image analysis package of choice for ourexpanding proteomics capabilities.’’Dr. Jens Coorssen, University of Calgarywww.perkinelmer.com/proteomics

Key Features & Benefits:High SensitivityA revolutionary new spot detection algorithm enables high sensitivity detection of50–100% more true spots than standard software packages. (see Figure 1.)Improved consistencyAutomatic warping algorithm is used to drive gel matching. Warping isthrough elimination ofindependent of spot detection allowing for automatic matching with minimalgel-to-gel variation requirement for seed placements. (see Figure 2.)Improved level of automationPowerful data visualization andcomparative analysis toolsLimited user input into set-up parameters through the Analysis Wizard enables simple,fast initialization of experiment analysis and eliminates user-to-user variability.Features like 3D spot view enable user to monitor and verify accuracy of automatedspot detection and matching.Figure 2A.Two protein gel images overlaid.Variation in spot positions of proteinsis mainly due to intrinsicvariation within the gel-runningprocess (gel matrix, gel size, bufferconcentrations, gel rig, etc.).Figure 2B.Gel images are warped andmatched. Where gels matchperfectly, spot color is eliminated.Spots of interest remain coloredas they are unique or up/down regulatedin one gel versus the other.Phoretix 2D Evolution is an integral component of the PRECISION PROTEOMICSsolution available from PerkinElmer Life Sciences.High-resolution gel images can be captured on the ProXPRESS imager and then analyzed using the high sensitivityPhoretix 2D Evolution software. A spot picking list is generated which can be read by the ProXcision spot excision robotto enable accurate, automatic spot excision of the proteins of interest.Ordering InformationCat. No.Product1442-320 Phoretix Evolution1442-320-1 Additional Phoretix Evolution licenseMinimum PC System requirements:P4/Athlon 1.7 GHz, 1 Gb memory,high power 3D card, 18 Gb hard drive,Windows ® 2000Worldwide Headquarters: PerkinElmer, Inc., 549 Albany Street, Boston, MA 02118-2512 USA (800) 551-2121European Headquarters: PerkinElmer, Imperiastraat 8, B-1930 Zaventem BelgiumTechnical Support: in Europe: techsupport.europe@perkinelmer.com in US and Rest of World: techsupport@perkinelmer.comBelgium: Tel: 0800 94 540 • France: Tel: 0800 90 77 62 • Netherlands: Tel: 0800 02 23 042 • Germany: Tel: 0800 1 81 00 32 • United Kingdom: Tel: 0800 89 60 46Switzerland: Tel: 0800 55 50 27 • Italy: Tel: 800 79 03 10 • Sweden: Tel: 020 79 07 35 • Norway: Tel: 800 11 947 • Denmark: Tel: 80 88 3477 • Spain: Tel: 900 973 255P11245Phoretix and Progenesis are trademarks of Nonlinear Dynamics, Ltd. Windows is a registered trademark of Microsoft Corporation.All other trademarks or registered trademarks are the property of PerkinElmer, Inc.© 2002 PerkinElmer, Inc.www.perkinelmer.com/proteomics