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Ion Total RNA-Seq Kit v2 User Guide (Pub. no. 4476286 ... - URGV

Ion Total RNA-Seq Kit v2 User Guide (Pub. no. 4476286 ... - URGV

Ion Total RNA-Seq Kit v2 User Guide (Pub. no. 4476286 ... - URGV

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2Chapter 2 Prepare Whole Transcriptome LibrariesFragment the whole transcriptome <strong>RNA</strong>Amount of Fragmented<strong>RNA</strong> in 3 µL• ≥50 ng of poly(A) <strong>RNA</strong>• ≥100 ng of r<strong>RNA</strong>-depletedtotal <strong>RNA</strong>• ≥100 ng of WT Control <strong>RNA</strong>•

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