BDâ„¢ P100 Blood Collection Kit
BDâ„¢ P100 Blood Collection Kit
BDâ„¢ P100 Blood Collection Kit
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BD <strong>P100</strong> <strong>Blood</strong> <strong>Collection</strong> <strong>Kit</strong><br />
Setting the Standard in Plasma Proteomics<br />
Explore
Minimize Preanalytical Variability<br />
with BD <strong>P100</strong> <strong>Blood</strong> <strong>Collection</strong> <strong>Kit</strong><br />
Human plasma contains a wide variety of proteins<br />
that hold potential as biomarkers for proteomics-based<br />
diagnostic and prognostic assays. * These proteins are<br />
subject to proteolytic degradation and modification<br />
during and after blood collection. The use of protease<br />
inhibitors – mixed immediately with blood – minimizes<br />
preanalytical variability associated with plasma sample<br />
preparation and handling.<br />
BD <strong>P100</strong> v1.1 <strong>Blood</strong> <strong>Collection</strong> <strong>Kit</strong> enables greater<br />
recovery and preservation of plasma<br />
proteins by immediately mixing with<br />
proprietary novel on-board<br />
stabilizers, specifically formulated<br />
for human plasma.<br />
Preserve Valuable Plasma Proteins<br />
† Patented<br />
* BD <strong>P100</strong> is For Research Use Only – Not For Use in Diagnostics Procedures<br />
Highlights:<br />
• On-board stabilizers provide point-of-collection<br />
protection of plasma proteins<br />
• Enhances long-term preservation by stabilizing<br />
analytes during blood collection<br />
• Innovative mechanical separator † minimizes<br />
cellular contamination<br />
• Eliminates pre-mixing and transfer tubes<br />
• Sterile and ready to use<br />
BD <strong>P100</strong> <strong>Blood</strong> <strong>Collection</strong> Tube Specifications:<br />
Total <strong>Blood</strong> Draw Volume: ~8.5 mL<br />
Plasma Yield: ~3.5 mL<br />
Anticoagulant (Spray Dried): K 2 EDTA<br />
Additive (Lyophilized): Proprietary Protein Stabilizers<br />
Storage Prior to Use: 2°C to 8°C
BD <strong>P100</strong> v1.1 Immediately Stabilizes Plasma Proteins<br />
BD <strong>P100</strong> v1.1 versus EDTA Plasma<br />
“Time 0” Gel Plot Summary of Eight Subjects (m/z 1000-2400)<br />
BD <strong>P100</strong> v1.1 versus EDTA Plasma<br />
“Time 0” Zoom Plot (m/z 1700-2050)<br />
Figure 1. Preservation of plasma proteins with BD <strong>P100</strong><br />
v1.1 <strong>Blood</strong> <strong>Collection</strong> <strong>Kit</strong>. <strong>Blood</strong> was drawn directly from<br />
eight subjects into <strong>P100</strong> and EDTA evacuated tubes. Plasma<br />
was processed within 10 minutes of blood collection by<br />
centrifugation at 2,500 x g for 15 minutes at room temperature.<br />
After blood collection, plasma was transferred into microtubes<br />
and prepared for mass spectrometry analysis or stored at –80ºC<br />
for future analysis. Peptides were isolated from the plasma<br />
samples and analyzed by MALDI-TOF MS. The mass spectra<br />
were collected in the mass range from 800 to 3200 m/z. Areas<br />
within the red rectangles demonstrate enhanced peptide<br />
stabilization in the <strong>P100</strong> samples versus EDTA plasma samples.<br />
A greater degree of proteolytic degradation is also more<br />
apparent in the EDTA plasma samples, even though samples<br />
were processed immediately upon blood collection (“Time 0”),<br />
as shown in the yellow rectangle areas.<br />
Figure 2. Enhanced stability of peptides observed in<br />
plasma samples collected with BD <strong>P100</strong> v1.1 <strong>Blood</strong><br />
<strong>Collection</strong> <strong>Kit</strong>. Regions with red arrows on this<br />
MALDI-TOF MS zoom plot (m/z 1700-2050) of two<br />
subjects demonstrates the ability of <strong>P100</strong> v1.1 to<br />
stabilize plasma peptides more effectively in the<br />
presence of protease inhibitors. These peaks are absent<br />
in the same regions on the EDTA zoom plots for both<br />
subjects. The immediate mixing of blood with the onboard<br />
protease inhibitors in BD <strong>P100</strong> tubes enhances<br />
the recovery of plasma proteins for proteomics studies.
Quantity Description<br />
12<br />
12<br />
12<br />
12<br />
<strong>Kit</strong> Contents<br />
BD <strong>P100</strong> <strong>Blood</strong> <strong>Collection</strong> Tubes<br />
Six (6) tubes/foil pouch, Two (2) foil pouches/kit<br />
BD Vacutainer ® One Use Tube Holders<br />
Single Use Only, Translucent<br />
BD Vacutainer ® Safety-Lok <strong>Blood</strong> <strong>Collection</strong> Set<br />
21 G Butterfly Needle, 12” (304.8 mm) Tubing<br />
Sterile, Use Once and Discard<br />
BD Alcohol Swab: Isopropyl Alcohol - 70%<br />
(Reference #366455 Only)<br />
1 Instructional Product Insert<br />
1 Research Use Only Certification Card<br />
North America<br />
#366455<br />
Reference Numbers<br />
Outside North America<br />
#366456
Ordering and Technical Information *<br />
Region Ordering Technical<br />
US<br />
888.237.2762 (phone orders)<br />
800.847.2220 (fax orders)<br />
800.631.0174<br />
(8:30 am – 5 pm) EST<br />
Canada 800.268.5430 ext. 5567 800.268.5430 ext. 5567<br />
All other<br />
areas<br />
Please visit www.bd.com<br />
to locate your local regional office<br />
To obtain more information on BD <strong>P100</strong> and other<br />
proteomics products, please visit our Web site<br />
www.bd.com/proteomics<br />
* BD <strong>P100</strong> is sold directly by BD Diagnostics – Preanalytical Systems<br />
Not available through distributors.
BD, BD Logo, and all other trademarks are property of Becton, Dickinson and Company. © 2007 BD<br />
03/07 VS7530-1<br />
1 Becton Drive<br />
Franklin Lakes, NJ 07417<br />
www.bd.com
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