BD BioCoat Matrigel Invasion Chamber - BD Biosciences

More documents

Recommendations

Info

SPC-354480-G rev 9.0 Release Date: 9/14/01 TABLE OF CONTENTS Page 1 Intended use .................................................................................................................................. 2 Summary ....................................................................................................................................... 2 Materials Provided. ........................................................................................................................ 2 Materials Required But Not Supplied............................................................................................. 2 Precautions.................................................................................................................................... 3 Procedure for Use.......................................................................................................................... 3 1.0 Rehydration.............................................................................................................. 3 2.0 Invasion Studies..................................................................................................... 4 3.0 Measurement of cell invasion ................................................................................ 4 Typical results................................................................................................................................ 7 Stability .......................................................................................................................................... 7 Technical Service........................................................................................................................... 7 References .................................................................................................................................... 8 NOTE: Process changes have been made to improve the performance and quality of BD BioCoat Matrigel Invasion Chambers.* Please review the following Sections for new information regarding product usage. PRECAUTIONS (b) (page 3): New storage conditions PROCEDURE FOR USE (page 3): Appearance of the Matrigel Basement Membrane Matrix PROCEDURE FOR USE, Section 1.0 Rehydration (page 3) New rehydration times PROCEDURE FOR USE, Section Invasion Studies (page 4): New suggested cell seeding densitiies * US Patent pending
INTENDED USE SPC-354480-G rev 9.0 Release Date: 9/14/01 Page 2 The BD BioCoat Matrigel Invasion Chamber is useful to study cell invasion of malignant and normal cells. Specific applications include assessment of the metastatic potential of tumor cells 1 , inhibition of metastasis by extracellular matrix components 2 or antineoplastic drugs (taxol) 3 , altered expression of cell surface proteins 4 or metalloproteinases 5 in metastatic cells, and invasion of normal cells, such as embryonic stem cells 6 , cytotrophoblasts 7 , and fibroblasts 8 . Invasion studies have been successfully performed on a variety of tumor cells (cell lines and primary tumors) including melanomas, glioblastomas, astrocytomas, osteosarcomas, fibrosarcomas, and adenocarcinomas of the lung, prostate, breast, ovary, and kidney. This product is FOR RESEARCH USE ONLY; NOT FOR USE IN DIAGNOSTIC PROCEDURES. SUMMARY BD BioCoat Matrigel Invasion Chambers provide cells with the conditions that allow assessment of their invasive property in vitro. The BD BioCoat Matrigel Invasion Chambers consists of a BD Falcon TC Companion Plate with Falcon Cell Culture Inserts containing an 8 micron pore size PET membrane with a thin layer of MATRIGEL Basement Membrane Matrix. The Matrigel Matrix serves as a reconstituted basement membrane in vitro. The layer occludes the pores of the membrane, blocking non-invasive cells from migrating through the membrane. In contrast, invasive cells (malignant and non-malignant) are able to detach themselves from and invade through the Matrigel Matrix and the 8 micron membrane pores. The membrane may be processed for light and electron microscopy and can be easily removed after staining. The MATRIGEL Invasion Chamber is a convenient, ready-to-use system to study cell invasion in vitro. MATERIALS PROVIDED • Invasion Chambers (24 inserts each) • Catalog # 354480 provided as 12 inserts in each of two 24-well BD Falcon TC Companion Tissue Culture Plates • Catalog # 354481 provided as 6 inserts in each of four 6-well BD Falcon TC Companion Tissue Culture Plates MATERIALS REQUIRED BUT NOT SUPPLIED • Bicarbonate based culture medium such as DMEM (serum-free) • Control Inserts (24-well, Catalog #354578 or 6-well, Catalog #354576) • Chemoattractant such as 5% fetal bovine serum in tissue culture medium • Companion Plates (24-well, Catalog #353504 or 6-well, Catalog #353502) • Humidified tissue culture incubator, 37 o C, 5% CO2 atmosphere
Page 1: BD BioCoat Matrigel Invasion Chambe
Page 5 and 6: SPC-354480-G rev 9.0 Release Date:
Page 7 and 8: SPC-354480-G rev 9.0 Release Date:
Page 9: REFERENCES 1. Albini, A. et al., Ca

BD BioCoat Matrigel Invasion Chamber - BD Biosciences

Create successful ePaper yourself

Delete template?

Save as template?