POPULATION ASSAY: PROAMP - Mesa Labs
POPULATION ASSAY: PROAMP - Mesa Labs
POPULATION ASSAY: PROAMP - Mesa Labs
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<strong>POPULATION</strong> <strong>ASSAY</strong>:<strong>PROAMP</strong>Total @ 24 hrs / number of plates counted x DF x AVF = CFU/ampouleDF= Dilution factor (absolute value of the reciprocal of the dilution)AV= Average number of colonies per ampouleAFV=Ampoule fill volumeIncubation End Time: __________________________# dilutions __________CFU COUNTS AT 24 HOURSPlates 1._____________ 2._____________ 3._____________Total @ 24hours: ______________________Total @ 24 hrs__________ / 3 x _________(DF) x ________(AFV) =______________(AV)CFU/ampoule__________________________________________________________________________________________CFU COUNTS AT 24 HOURS# dilutions __________Plates 1.____________ 2._____________ 3.______________Total @ 24 hours: ____________________Total @ 24 hrs__________ / 3 x __________(DF) x ________(AFV)=______________(AV)CFU/ampoule# of Dilutions = Dilution Factor1 = 102 = 1003 = 10004 = 100005 = 1000006 = 1000000Sum of the AV of both dilutions / 2 =CFU/ ml_________________ / 2 =_____________________________________x10CFU/ampouleRead By:_________________________________________________Date:____________________LWI-1519 Rev 2 Effective: March 15, 2010Raven <strong>Labs</strong> Page 2 of 2Omaha, NE
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