Scientific <strong>Poster</strong>s – <strong>Poster</strong> <strong>Session</strong> 2<strong>Poster</strong> #151A NOVEL EPITHELIOID SARCOMA CELL LINEHAS CANCER- STEM LIKE PROPERTIES BASEDON HIGH ALDEHYDE DEHYDROGENASE 1 A1(ALDH1A1) ACTIVITYMakoto Emori, MD 1 ; Tomohide Tsukahara 2 ; Masanobu Kano 3 ;Toshihiko Torigoe 2 ; Toshihiko Yamashita 1 ;Noriyuki Sato 2 ; Takuro Wada 11Orthopedic Surgery, Sapporo Medical University, Schoolof Medicine, Sapporo, Japan; 2 Pathology, Sapporo MedicalUniversity, School of Medicine, Sapporo, Japan; 3 OrthopedicSurgery, Chitose City Hospital, Chitose, JapanObjective: Epithelioid sarcoma (ES) is a relatively rare softtissue sarcoma accounting for
Scientific <strong>Poster</strong>s – <strong>Poster</strong> <strong>Session</strong> 2Conclusion: Expression of CUL4A gene shows a prognosticrole in overall survival in high risk localized STS patients.The outcome of this exploratory analysis is alignedwith our hypothesis that genes implicated in cell cycleregulation and chromosome stability could play a relevantprognostic role in STS.Methods: Fresh tumour material was obtained from 38consecutive patients with an aggressive benign or malignantbone or soft-tissue tumour. One million single cellsderived from either patient material or established cell lineswere suspended in 100 µl matrigel and were placed on theCAM. Histological evaluation and scoring of graft and hostbehaviour was performed by three observers according toprotocol. The effect of several kinase-inhibitors, one MPPinhibitorand physical treatment (irradiation and freezing)on the cell lines was tested by means of an MTT-test, acollagen type1 invasion test and the CAM-assay.Results: For the patient samples correct histological diagnosisremained possible after harvesting of the CAM. Lessthan half of the tumour samples proved to be viable, withmarked differences between patients. Preoperative chemotherapyhad an adverse effect on tumour invasiveness.Metastatic lesions proved to be very viable and showedmarked vascular ingrowth. Infiltration of fibroblasts fromthe CAM into the tumour samples is thought to heraldvascular ingrowth. Macroscopic vascular reaction did notcorrelate with microscopic vascular ingrowth. Significanteffect of the inhibitors, irradiation and freezing on sarcomacell proliferation and invasion could be established for thedifferent cell lines in all three tests.Conclusion: The CAM assay can be used to study freshmaterial derived from tumours of the musculoskeletalsystem and proved to be a useful tool for the evaluationof treatment efficacity on sarcoma proliferation and invasion.<strong>Poster</strong> #153THE CAM-ASSAY IS A USEFUL PRECLINICALPREDICTIVE AND PROGNOSTIC MODEL FORSARCOMAGwen M. Sys, MD 1 ; Nikita Stevens 1 ; Ramses Forsyth 2 ;Lore Lapeire 4 ; Bart Poffyn 1 ; Marc Bracke 3 ; Olivier De Wever 31Orthopaedic Surgery, Ghent University Hospital, Ghent,Belgium; 2 M. Ghoormaghtigh Institute of Pathology, GhentUniversity Hospital, Ghent, Belgium; 3 Radiation <strong>Oncology</strong>and Experimental Cancer Research, Ghent University,Ghent, Belgium; 4 Medical <strong>Oncology</strong>, Ghent UniversityHospital, Ghent, BelgiumObjective: Sarcomas are very rare, while recent developmentin cancer treatment has generated multiple newmolecules, a combination necessitating preclinical modelsfor the evaluation of therapeutic efficacity. In this study,fresh tumour samples, single cell suspensions of patientmaterial, and cell lines (SAOS2 and SW1353) were usedto establish whether the chick chorioallantoic membrane(CAM) assay can be used to evaluate the viability of thesetumours and to examine their migrational, invasive andangiogenetic behaviour.<strong>Poster</strong> #154PATHWAY ANALYSIS IN UNDIFFERENTIATEDPLEOMORPHIC SARCOMA SIDE POPULATIONCELLS REVEALS AN IMPORTANT ROLE FORWNT SIGNALING INHIBITION IN TUMORSELF-RENEWALQingXia Wei 1 ; Veronique Voisin 2 ; Ilkyu Han 3 ; Hao Li 1 ;Cassandra Tyson 1 ; Shingo Sato 1 ; Peter Dixon 1 ;Mushriq Ali Jazrawe 1 ; Makato Hirata 1 ; Gary Bader 2 ;Benjamin Alman 1 ; Jay Wunder 41Developmental and Stem Cell Biology, Hospital for SickChildren, Toronto, ON, Canada; 2 The Donnelly Centre,University of Toronto, Toronto, ON, Canada; 3 OrthopaedicSurgery, Seoul National University Hospital, Seoul,Democratic People’s Republic of Korea; 4 Surgery,Mount Sinai Hospital, Toronto, ON, CanadaObjective: Undifferentiated pleomophic sarcomas (UPS)contain a small subpopulation of tumor initiating cells(TICs) that can be identified using the side population(SP) assay. The TICs are over 1,000-fold enhanced for theability to form tumors when transplanted into NOD/SCID245