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Sandler Asthma Basic REsearch CenterCore FacilitiesCell Sorting and Analysis CoreDirector: Zhien-WangObjectiveFlow cytometry is necessary to characterize, purify and separate cell types based on surfacecharacteristics. The objective of this core is to provide technical support and access to highlysophisticated instruments for persons at UCSF performing asthma-related research.Contributions from SABRE Center have been partnered with the Diabetes Center, individualinvestigator resources and institutional resources to support the space and maintenance of thecore largely through an integrated re-charge system. Students and postdocs participate intraining in the core at an area where training can be centralized in order to facilitate maintenanceand oversight.AccomplishmentsThe Core is located in S1067 in proximity to Dr. Locksley’s laboratory and centrally within theImmunology Program corridor above the SABRE Center space in HSE201. Scheduling is onlineand suitably trained individuals can access instruments in the core 24 hours a day, 7 days aweek.The space offers an array of instrumentation that offers unique capabilities in data acquisitionand enhances the depth of technical capacity:Two Beckman-Coulter MoFlo XDP High-Speed sorters with 4-laser, 17-color, 15-parametercapability centered around 488 nm, 532 mm, 561 mm and 647 nm multi-line air-cooled lasers.Two side-by-side instruments has enabled operation by a single individual, thus optimizing useand minimizing cost.Becton-Dickinson Biosciences LSR II Flow Cytometer with 4-laser, 10-color, 12-parametercapability centered around 488 nm OPSL, 637 nm (red), 403 nm (violet) and 535 nm (green)lasers. Total use > 1600 hrs.Becton-Dickinson Biosciences LSR II Flow Cytometer with 4-laser, 10-color, 12-parametercapability centered around 488 nm OPSL, 637 nm (red), 403 nm (violet) and 535 nm (green)lasers is maintained by the core but stationed in HSE201 in the SABRE core space. Total use >800 hrs.Training and Integration with Sandler ProgramUse of the core has stabilized over the past 2 years. Over the last 12 months, >45 laboratories atthe UCSF Parnassus and Mission Bay campuses have used the facility 2 or more times, with 32labs over 3 times. In turn, the numbers of users per laboratory has also stabilized at 2.3 users/lab,and reflects use by graduate students and postdocs. Nine of the labs are direct participants in theSABRE Center, and a number of the other users are affiliated investigators examining aspects of49