High-performance liquid chromatographic determination of bergenin ...

elution were not observed (match factors ≥95%). Linearity and limits ofdetection (LOD) and quantification (LOQ) were evaluated for quantitativepurposes (Table II). LOD for bergenin and gallic acid were 1.16 and 0.66µg mL −1 and LOQ were 3.9 and 4.2 µg mL −1 , respectively, implying themethod was suitable for quantification of these compound. R 2 values forthe compounds were >0.98, confirming the linearity of the method. Themethod can therefore be regarded as suitable for quality control and standardizationof Bergenia species.Table IIRetention times, correlation coefficients and linear range from regression analysis, andlimits of quantification (LOQ) and detection (LOD)Compound t R (min) a R 2 Linear range LOD LOQ(µg mL −1 ) (µg mL −1 ) (µg mL −1 )Gallic acid 5.91 ± 0.12 0.990 10–200 0.66 4.2Bergenin 11.06 ± 0.23 0.957 5–50 1.16 3.9a Means from ten replicates ± SDThese results suggest B. ciliata and B. stracheyi are better sourcesof bergenin than B. ligulata and that their biological activity will be greater.ACKNOWLEDGEMENTThe authors are grateful to Dr Rakesh Tuli, Director, NBRI forproviding the facilities to conduct this research work.REFERENCES[1] L.V. Asolkar, K.K. Kakkar, and O.J. Chakre, Glossary of IndianMedicinal Plants with Active Principles. PID, CSIR, New Delhi,1992, p. 122[2] K.K. Singh, Indian Int. J. Pharmacol., 35, 105 (1997)[3] S.K. Kapur, J. Econ. Tax. Bot., 17, 395 (1993)[4] P.B. Singh and B.S. Aswal, Bull. Med. Ethnobot. Res.,13, 172 (1992)- 251 -