DNA quantification and normalization

∗ Pour the prepared PicoGreen working solution into a BCFullReservoir. Run themethod “PicoGreen_Stampytsning_6x96_sBCFullReservoir_date” (D: > ProgramFiles > Biomek FX > Methods > Normalization), which will dispense 150µl of thesolution into each well. See figure below. Save the solution left over in a falcon tubeand put it in the -20 freezer.Transfer 1.5µl of your DNA to the PicoGreen stem plate∗ First make sure that none of your samples are empty due to evaporation. Run atransfer method in folder D: > Program Files > Biomek FX > Methods >Normalization, choose method after number of DNA plates (e.g. “Transfer_1.5µl3xDNA to PicoGreen plate”).∗ After the transfer you need to add standard curve samples to the titer plate, to be ableto calculate the concentrations of your unknown samples. Use Ceph DNA with aknown concentration of 50ng/ul to make the standard curve.Biomek FX and 7900 HT090116/CN