Proficiency testing catalogue - LGC Standards

Proficiencytestingcatalogue

Introduction4Beverage PT schemes32• LGC Standards• Aim of proficiency testing• Quality standards• Benefits of proficiency testing• Who should participate in proficiencytesting schemes?45555Selector chart• QBS - Beverage scheme• BAPS - Brewing analytes scheme• DAPS - Distillers’ analytes scheme• MAPS - Malt analytes scheme• SUPS - Sugar scheme323435363738• Who participates in LGC Standardsproficiency testing schemes?• Why choose LGC Standards as yourproficiency testing provider?• Why do I need proficiency testing?666Other PT schemesSelector chart• PACQS - Particle analysis andcharacterisation quality scheme404042Typical scheme cycle from start to finishPORTAL Proficiency Online Reportingand Trend AnaLysis78• PHARMASSURE - Pharmaceuticalscheme• PHYTAS - Phytochemical scheme4344Food PT schemesSelector chart• AFPS - Animal feeds scheme• QCS - Chocolate scheme10101213• QMIS - Microbiology investigationscheme• QUARTZ - Forensic blood toxicologyscheme• TOYTEST - Toy safety scheme454647• QDCS - Dairy chemistry scheme• QFCS - Food chemistry scheme• QGS - Gelatine scheme• QMAS - Meat scheme• QMS - Food microbiology scheme1415161718Other services• Special bespoke microbiologytest materials• Closed customised PT schemes• Other PT services49505151Water and Environment PT schemesSelector chart• Aquacheck - Water chemistryscheme202022• Product development• Reference materials• Training and educationSummary51515252• CONTEST - Contaminated landscheme• STACKS - Stack emissions scheme2628Frequently asked questions53• QWAS - Water microbiology scheme29LGC Standards offices63Disclaimer: Whilst every effort is made to keep this document up to date and accurate, please check with LGC Standards Proficiency Testing or reviewthe current application forms and scheme descriptions available at www.lgcpt.com

Introduction• LGC Standards• Aim of proficiency testing• Quality standards• Benefits of proficiency testing• Who should participate inproficiency testing schemes?• Who participates in LGC Standardsproficiency testing schemes?• Why choose LGC Standards asyour proficiency testing provider?• Why do I need proficiency testing?• Typical scheme cycle from startto finish• PORTAL Proficiency OnlineReporting and Trend AnaLysis

IntroductionLGC StandardsPromoting excellence throughproficiency testingLGC Standards is a UKAS accreditedinternational provider of proficiency testing(PT) services. We have over twenty five yearsexperience in all aspects of providing proficiencytesting services to laboratories undertakingchemical, microbiological and physicalmeasurements.We offer an unprecedented breadth of chemical,microbiological and physical testing schemesacross a wide range of industries including meat,dairy and other food sectors, water, soil andother environmental sectors, brewing, distilling,malting, sugar, forensic, consumer safety,pharmaceutical and phytochemical sectors.Schemes are supplied on an international basiswith customers in over 130 countries makingLGC Standards a major international providerof proficiency testing services.LGC Standards operates 29 proficiency testingschemes serving over 6,000 laboratories.We produce and distribute more than 100,000test materials and process more than 2,000,000data points per annum.All the schemes within LGC Standards areoperated in accordance with the internationalstandard ISO/IEC 17043. LGC Standards is aUKAS (United Kingdom Accreditation Service)accredited PT provider (certificate number: 0001),our schemes currently being accredited againstISO/IEC 43-1 and ILAC G13. Please note thatthe AFPS, PACQS and PHYTAS schemes arecurrently not included in our scope ofaccreditation.In addition to the variety of schemes offered,we can also provide managed solutions forin-house proficiency testing providers andtraining for participants and their customers.4 Tel: +44 (0)161 762 2500 Fax: +44 (0)161 762 2501

IntroductionAim of proficiency testingBenefits of proficiency testingProficiency testing (PT) is defined in ISO/IEC17043 as the evaluation of participantperformance against pre-established criteriaby means of interlaboratory comparisons.LGC Standards Proficiency Testing provides awide range of schemes designed to improvethe quality of analysis in those sectors covered.The schemes involve the regular distributionof test materials, in order for participants to testfor defined parameters, and to have their resultsstatistically analysed. Participation provideslaboratories with a means of assessing theaccuracy and comparability of their results topeer laboratories over time.When performed within the context of acomprehensive quality assurance programme,proficiency testing is an independent meansof assuring the quality of test and calibrationresults, as described in ISO/IEC 17025.Quality standardsProficiency testing is an essential laboratorytool as it demonstrates a laboratory’scommitment to good performance and enablesparticipants to confirm their ability to performtests competently; essential in the laboratoryaccreditation process.Participation in proficiency testing will:• enable participants to measure theirperformance against others;• give an early indication of potential problemsor training requirements;• encourage good performance and reinforcean interest in quality assurance;• demonstrate an ability to comply withinternational regulations;• provide a valuable source of information;• provide the means to measure consistencyacross a group of laboratories.Who should participate in proficiencytesting schemes?LGC Standards Proficiency Testing is committedto continual improvement in quality and efficiencythrough procedures based upon qualityassurance. This commitment is demonstratedthrough certification to ISO 9001:2008 for all itsactivities and ISO/IEC 43-1 and ILAC G13 forthe operation, management and design ofproficiency testing schemes. LGC StandardsProficiency Testing is accredited by the UnitedKingdom Accreditation Service (UKAS) for theprovision of proficiency testing schemes. Acopy of our current scope is available on ourwebsite: www.lgcpt.comAnyone who needs to independentlydemonstrate the quality of their analytical resultsshould use PT schemes – because quality ofresults relates directly to quality of product,reputation in the market and, ultimately, brandvalue. Whether operating in the food,pharmaceutical, beverages, environmentalmonitoring and or other sectors, many regulatorsview PT schemes as an essential part of qualitymonitoring and many companies link PT resultsto their own key performance indicators in thequality assurance process.Email: customerservices@lgcpt.comWebsite: www.lgcpt.com5

IntroductionWho participates in LGC Standardsproficiency testing schemes?LGC Standards exports to laboratories in over130 countries world-wide and our customerbase ranges from single small enterprises toinspection organisations of global repute. Ourcustomers include government agencies, majorinternational food manufacturers, researchorganisations, commercial and contractlaboratories. Currently we have over 6,000participants across the group.LGC Standards operates a number of bespokeschemes for multi-national companies to meettheir particular requirements. These specialschemes cover up to 200 laboratories.Why choose LGC Standards as yourproficiency testing provider?• Access to a wide range of schemes froma single supplier;• Rapid turnaround of results;• Access to expert support and advice;• Local representation and support.Why do I need proficiency testing?Accreditation bodies strongly recommend thatlaboratories participate in appropriate PTschemes as they are the only quality tool whichcan assess the whole quality system. PT isa truly independent measure of laboratoryperformance and anonymously comparesperformance with peer laboratories. It allowsthe laboratory to compare and contrast theperformance of analytical methods and canassist in the validation of new methods.Participation in a PT scheme is educational andcan be used as a tool for staff training, allowinglaboratories to learn from both positive andnegative aspects.6 Tel: +44 (0)161 762 2500 Fax: +44 (0)161 762 2501

IntroductionTypical scheme cycle from start to finishProcurement, preparation, dispensing andquality control testing of test materials6 – 12 weeks priorDespatch of test materials and instructionsto participantsWeek 1Round reviewed and changesmade if requiredParticipants analyse test material and reportresults to LGC Standards Proficiency Testingas instructed and within the specified deadlineWeek 2 - 3Results analysed and the performance oflaboratories compared using appropriatetechniques, such as z-scoresWeek 4Report issued to the participantsWeek 4Reports are issued as soon as possible after the round closure, although thetimescale between closing dates and issue of final report will vary from schemeto scheme.Email: customerservices@lgcpt.comWebsite: www.lgcpt.com7

IntroductionPORTALProficiency Online Reporting and Trend AnaLysisPORTAL offers a single web based reportingsystem to be used across all LGC StandardsPT schemes providing improved fuctionalityand flexibility.There are a number of ways you can chooseto use PORTAL, whether you want to have onedesignated user to submit all your analysts’results, or each analyst logging their ownresults, or a combination of the two, it is allpossible. We advise that prior to using PORTALyou read the user guide which is available at:www.lgcpt.com/portal Select ‘help’ from themenu and decide how the system can be bestutilised within your organisation.• A single web based system encompassingall our PT schemes;• Functional, flexible and secure;• Results entry and reports available online24/7 - 365 days;• Analysis and reporting of results by method;• Intelligent importing of previously entered data;• Ability to compare analysts, methods orinstruments;• Customisable trending of results;• Choice of detailed, concise and Excel reports;• Define laboratory results and analyst results;• Complete management of your results priorto deadlines;• User management that ensures completetraceability;• Enables each analyst to take ownership oftheir result;• Measurement uncertainty reporting;Note: Website supports Internet Explorer version 6 or higher.Images displayed may differ from the final website.• Helpful support staff.8 Tel: +44 (0)161 762 2500 Fax: +44 (0)161 762 2501

• AFPSAnimal feeds scheme• QCSChocolate scheme• QDCSDairy chemistry schemeFoodPT schemes• QFCSFood chemistry scheme• QGSGelatine scheme• QMASMeat scheme• QMSFood microbiology scheme

Food PT scheme selectorFoodPT schemesScheme Scheme year Tests Test material matrixAFPS* April - March Chemical and Animal feed (Various e.g. broiler,Animal feeds scheme microbiological cattle, pig), calf replacer, premix,oil and lardQCS January - December Chemical and Chocolate andChocolate scheme microbiological cocoa powderQDCS January - December Chemical Butter, cheese, cream, milk,Dairy chemistry schememilk powder, whey powder,yoghurt and standard solutionsQFCS January - December Chemical Cereals, *fruit/vegetables,Food chemistry scheme*‘ready to eat’ products andstandard solutionsQGS January - December Microbiological Gelatine hydrolysateGelatine schemeQMAS January - December Chemical and Meat and fishMeat schememicrobiologicalQMS January - December Microbiological Oatmeal and skimmedFood microbiologymilk powderscheme“Which international standards are relevant to PT?*Please note that the AFPS scheme fruits/vegetables and ‘ready to eat’ products are currently not included in our scope of accreditation.All the PT schemes within LGC Standards Proficiency Testing are operated inaccordance with the international standard ISO/IEC 17043.The statistical analysis undertaken is in accordance with the international standardISO 13528. LGC Standards Proficiency Testing is accredited by the United KingdomAccreditation Service for the provision of proficiency testing schemes, currentlyagainst ISO/IEC Guide 43.1 and ILAC G13. A copy of our current scope ofaccreditation is available on the UKAS website (www.ukas.com).”10 Tel: +44 (0)161 762 2500 Fax: +44 (0)161 762 2501

Analyte group Number of distributions See pageper scheme yearComprehensive range of chemical and microbiological Four 12analysis of animal feeds covering the major analytical areasincluding proximate analysis, trace elements, PCB/OCpesticides, aflatoxins, Salmonella spp., and indicator organisms.FoodPT schemesChemical parameters of relevance to the chocolate and Three 13food testing industries. Microbiological tests includingpathogens and indicator organisms.A variety of parameters ranging from classical wet Four 14chemistry to instrumentation regularly used within dairyand food testing laboratories.Food additives (preservatives, sweeteners, colours), Three 15nutritional analysis and pesticides.Microbiological tests including pathogens and indicator Two 16organisms of relevance to gelatine.Proximate analysis of relevance to the meat, fish and food Six 17testing industries, and contaminants of relevance to fishproducts. Microorganisms of relevance to meat and fishproducts, including pathogens and indicator organisms.Comprehensive range of microorganisms of relevance to Twelve 18food products, including pathogens, indicator organismsand spoilage organisms.0001Email: customerservices@lgcpt.comWebsite: www.lgcpt.com11

AFPS - Animal feeds schemeFoodPT schemesThe Animal Feeds Proficiency Scheme (AFPS)is specifically designed to meet the needs oflaboratories performing chemical ormicrobiological analysis of animal feedstuffs.Animal feed quality is highly regulated sincethe majority of animals, or their products, willbe converted into food for human consumption.EC Regulation 767/2009 stipulates therequirements for the marketing and use of animalfeeds, and (with reference to related regulations)requires complete traceability of feeds, personnel,and additives, and defines the compulsorylabelling requirements. Measures must betaken to prevent contamination with hazardousmaterials above specified limits; such asPCB's, heavy metals, mycotoxins, pesticidesetc. and microorganisms capable of causinghuman disease (zoonotic agents) such asSalmonella species.Major food safety crises can occur from thecontamination of animal feed causing risks toanimal and human health resulting in recalls;financial damage as large quantities of producthave to be destroyed; and the potential forconsiderable damage to the reputation of anybusinesses involved. Recent recalls includemelamine in pet foods, numerous events ofanimal feed cargo rejected due to aflatoxincontamination, pork products contaminatedwith dioxins, and many more. Preventing theseissues through reliable analysis at sourcebacked up by independent verification throughparticipation in a proficiency scheme, can helpto save large sums of money for a very smallinvestment.The full range and availability of test materialsin AFPS is determined on annual basis andfurther details can be found in the AFPSapplication form and scheme description.Test materialAnimal feed (Various e.g. broiler,cattle, pig), Calf Replacer,Premix, Oil and LardAnalytesChemical:Proximate Analysis:ADF (Acid detergent fibre), Ashinsoluble in Hydrochloric Acid,Crude Ash, Crude Fat, CrudeFibre, Crude Protein, Moisture,NDF (Neutral Detergent Fibre),PPD (Pepsin Protein Digestibility),Starch, Sugars.Minerals and Trace Elements:Arsenic, Cadmium, Calcium,Chloride, Chromium, Cobalt,Copper, Iron, Lead, Magnesium,Manganese, Mercury, Phosphorus,Potassium, Selenium, Sodium, Zinc.Fat Quality:Eluable Fat, Fatty Acids Profile(absolute), Fatty Acids Profile(relative), Free Fatty Acids,Insoluble Impurities, Moisture,Polymeric triglycerides,Unsaponifiable Matter.PCB and OC Compounds:PCB 28, PCB 52, PCB 101,PCB 118, PCB 138, PCB 153,PCB 180.cis-Chlordane, trans-Chlordane,p,p’-DDE, o,p’-DDT,p,p’-DDT, p,p’-TDE,β-Hexachlorocyclohexane,γ-Hexachlorocyclohexane,Hexachlorobenzene.Aflatoxins:Aflatoxin B1, Aflatoxin B2, AflatoxinG1, Aflatoxin G2, Ochratoxin.Microbiological:Enumeration:Enterobacteriaceae,Total aerobic mesophilic count,Yeast and Mould.Presence/absence:Salmonella species.Note: Test materials and analytes may be added or removed, pleasesee current application form.12 Tel: +44 (0)161 762 2500 Fax: +44 (0)161 762 2501

QCS - Chocolate schemeThe Quality in Chocolate Scheme (QCS) is aPT scheme intended for use by microbiologistsand chemists working in the chocolatemanufacturing industry.Cacao trees were first cultivated by the Aztecs,who used the beans both as a form of currencyand to produce a spiced drink known as‘chocolatl’. Centuries later, the first chocolatecompany was established in North America in1765. Over the years, many different types andflavours of chocolate have been developed andcacao derivatives are often blended with otheringredients to produce a diverse range ofproducts. Chocolate is probably the most popularend-product; in some countries up to 90% of thepopulation buys chocolate on a regular basis.Chocolate products are also one of the few foodcommodities whose composition is controlled atEC level. For example, EC Directive 2000/36/EC‘relating to cocoa and chocolate productsintended for human consumption’, sets commonrules and definitions with regard to thecomposition, manufacture, packaging andlabelling of chocolate and cocoa products.Test materialAnalytesChemical:Ash, Butyric Acid, Fat, Moisture,Theobromine, Total Nitrogen,Total Sugars.The microbiological problems associated withchocolate and cocoa products are unique to thechocolate industry, and are related to the lowwater activity and the high fat and sugar content.The most important microbiological risk liesin the possible presence of Salmonella species,which can survive in chocolate for long periodsof time and may not be destroyed by processing.Levels of spoilage organisms throughout themanufacturing process are also of interest froma commercial viewpoint, to prevent taste andappearance defects.The microbiological test materials are availablein chocolate or cocoa powder matrices. Testmaterials may or may not contain the targetorganisms, along with relevant background flora.Chemical analysis is equally important to ensurethe organoleptic properties are what we expect.After all, it’s why we consume so muchchocolate. Tests for moisture, fat, ash and totalsugars are important to ensure the right textureand mouth feel, whilst theobromine and butyricacid from the cocoa powder are key contributorsto the taste.The chemical test materials are available inchocolate or cocoa powder matrices. Levelsfor each analyte are generally representativeof the values typically found in the chocolatemanufacturing industry and the levels can usuallybe assumed to be in line with the regulationsgoverning them.FoodPT schemesChocolate and Cocoa PowderMicrobiological:Enumeration:Coliforms, Enterobacteriaceae,Enterococci, Total aerobicmesophilic count.Presence/absence:Salmonella species.The full range and availability of test materialsin QCS is determined on an annual basis andfurther details can be found in the QCSapplication form and scheme description.Note: Test materials and analytes may be added or removed, pleasesee current application form.Email: customerservices@lgcpt.comWebsite: www.lgcpt.com13

QDCS - Dairy chemistry schemeFoodPT schemesThe Quality in Dairy Chemistry Scheme (QDCS)is probably the most comprehensive proficiencytesting scheme available to laboratoriesperforming compositional and safety analysis inthe dairy sector. Participation in QDCS can helpgive authority to the results produced by yourlaboratory.For the dairy sector, EU Regulation 853/2004introduces specific hygiene rules applying toraw milk and dairy products, including controlof residual antibiotics.QDCS provides a wide range of test materials,covering numerous relevant analytical testparameters. These include traditional ‘wet’chemistry techniques, as well as determinationsby infrared analysers, which are widely used inthe dairy sector.A choice of real dairy foods including butter,cheese, cream, milk, whey powder and yoghurtare available, plus lyophilised test materials forthe detection of antibiotics.Participants choose what test materials toreceive at a frequency to meet their quality andaccreditation needs.The full range and availability of test materialsin QDCS is determined on an annual basisand further details can be found in the QDCSapplication form and scheme description.Test materialMilk:(Freeze dried, Skimmed,UHT, Whole)ButterCheese:(Hard, Soft)Milk Powder:(Skimmed, Whole)Whey PowderWhey Protein ConcentrateCream:(Single, Double, Whipping)YoghurtStandard Solutions:(Buffer solution, HCl, Potassiumhydrogen phthalate)AnalytesAntibiotics (penicillin and/orcephalosporin), Antibiotics(penicillin and/or sulphur-based),Calcium, Fat, Freezing Point,Lactose, Protein, Phosphatase,pH, Total Solids, Titratable Acidity.Moisture, pH, Salt.Calcium, Fat, Moisture, pH,Protein, Salt.Ash, Fat, Insolubility Index,Minerals (calcium, potassium,copper, sodium, chloride, iron,magnesium, manganese,phosphorus, zinc). Moisture,Protein, Scorched Particles,Titratable Acidity, WPNI.Ash, Fat, Lactose, Moisture,Protein, Scorched Particles.Ash, Bulk Density, Fat, InsolubilityIndex, Lactose, Moisture, pH,Protein.Fat, Moisture, Titratable Acidity.Fat, Protein, Total Solids.Acid Titration, COD, pH.Note: Test materials and analytes may be added or removed, pleasesee current application form.14 Tel: +44 (0)161 762 2500 Fax: +44 (0)161 762 2501

QFCS - Food chemistry schemeThe Quality in Food Chemistry Scheme (QFCS)is designed specifically for the determinationof additives used extensively in food andbeverage manufacturing, such as colours andpreservatives, and for basic nutritional analysis.QFCS is intended to provide these parameterson a regular basis and a minimum frequencyof three rounds per annum.Natural ‘additives’ in the form of salting andsmoking have been used for centuries. Additivesare often an important part of food production,enhancing food safety by inhibiting growth ofspoilage and potentially harmful organisms.Colours are often added to restore naturalcolour after processing, or on storage of foods,and to give the colour expected or preferred bythe consumer. Sweeteners are used to replacesugar in foods in order to produce lifestyleproducts such as diet or light versions of favouritefoods or to help consumers with medicalconditions, such as diabetes, avoid sugar.All food products require basic nutritionallabelling in order to help consumers makeinformed choices about the food they eat andare especially important where specific claimsare made about a food’s nutritional content.The use of additives such as preservatives,colours and sweeteners is closely controlledby legislation, in order to ensure only permittedtypes and levels are used within the productionof food. Whilst beneficial in the correct quantities,too much may be potentially harmful to healthin the long term and producers have no controlover the quantities of product an individual mayconsume. The relevant EU Directives governingthe use of additives in foods, and controllinglevels are Directive 94/35/EC for sweeteners;Directive 94/36/EC for colours; Directive 95/2/ECfor all other additives including preservatives(often referred to as ‘Miscellaneous Additives’);and Directive 90/496/EC for nutritional labelling.Test materialStandard SolutionsCereals and *’Ready to eat’products*Fruit/VegetableMost countries set strict limits for the amountof pesticide residue in foods in order to protectconsumers. The fruit/vegetable test material willallow participants to monitor their quality andreliability of results to help ensure compliancewith regulatory requirements.The full range and availability of test materialsin QFCS is determined on an annual basisand further details can be found in the QFCSapplication form and scheme description.AnalytesPreservatives:Benzoic Acid (E210 - E213)Sorbic Acid (E200 - E203)Sulfur Dioxide (E220 - E228)Sweeteners:Acesulfame K (E950)Aspartame (E951)Saccharin (E954)Colours:Allura Red (E129)Brilliant Blue (E133)Indigo Carmine (E132)Ponceau 4R (E124)Sunset Yellow (E110)Tartrazine (E102)Nutritional Analysis:Ash, Carbohydrate, Dietary Fibre,Energy, Fat, Moisture, Protein,Salt, Total Sugars.Pesticides:Herbicides, Organochlorine,Organophosphorus, Syntheticpyrethroids, Triazines.Note: Test materials and analytes may be added or removed, pleasesee current application form. *’Ready to eat’ products and fruit/vegetabletest materials are currently not included in our scope of accreditation.FoodPT schemesEmail: customerservices@lgcpt.comWebsite: www.lgcpt.com15

QGS - Gelatine schemeFoodPT schemesThe Quality in Gelatine Scheme (QGS) hasbeen developed in collaboration with the tradebody, Gelatine Manufacturers of Europe (GME).GME members account for nearly half of theworldwide gelatine production and the keyrole of the GME is to ensure that gelatine ismanufactured to a consistently high qualityfor the benefit of gelatine customers andconsumers.Gelatine is a pure protein obtained from animalraw materials containing collagen and is typicallyproduced in two grades as far as microbiologicalspecification is concerned – food grade andpharmaceutical grade.Gelatine is an excellent nutrient for most bacteriaand for this reason the manufacturing processeshave to avoid contamination otherwise thesafety and/or quality of the gelatine could beaffected. QGS test materials are available on agelatine hydrolysate matrix in order to representa realistic challenge, and include the relevantmicroorganisms for laboratories involved in thequality control analysis of gelatine. Test materialquantities are supplied as either 10 g forenumeration of indicator organisms or 25 g fordetection of pathogens.Test materialGelatine HydrolysateAnalytesEnumeration:Sulphite-reducing bacteria,Total aerobic mesophilic count.Presence/absence:Clostridium perfringens,Coliforms, Escherichia coli,Staphylococcus aureus,Salmonella species.Note: Test materials and analytes may be added or removed, pleasesee current application form.The full range and availability of test materialsin QGS is determined on an annual basis andfurther details can be found in the QGSapplication form and scheme description.16 Tel: +44 (0)161 762 2500 Fax: +44 (0)161 762 2501

QMAS - Meat schemeThe Quality in Meat Analysis Scheme (QMAS) isintended for use by chemists and microbiologistsworking in the meat and meat productsindustries. The scheme is also suitable for usein the fish processing industry, by the inclusionof a fish test material. Legislation in the formof EU Regulation 882/2004 ‘Official ControlsPerformed to Ensure the Verification ofCompliance with Feed and Food Law, AnimalHealth and Animal Welfare Rules’ requiresofficial control laboratories to be accredited toISO/IEC 17025 and therefore to use externalmeans of monitoring performance such asproficiency testing. In practice, many industrylaboratories and third party (contract) laboratoriesare also accredited and will use QMAS as ameans of monitoring the quality of analysis.For microbiological determinations, test materialsare provided in a lyophilised meat matrix. Testmaterials are available for the analysis ofindicator organisms, as performed routinely infactory based laboratories and off-site contracttesting facilities. There are also test materialsfor pathogen analysis suitable for laboratoriespermitted to undertake this analysis, often carriedout by external providers.The full range and availability of test materialsin QMAS is determined on an annual basisand further details can be found in the QMASapplication form and scheme description.FoodPT schemesA variety of real meat test materials in a vacuumpacked format is provided for participants,enabling them to analyse a comprehensiverange of relevant chemical tests which are allwidely used in the meat and food sectors. Thereal fish test material allows laboratories toperform a range of basic proximate analyses(fat, ash, salt, protein and moisture), and thelyophilised meat test material provides a highlystable matrix for the analysis of nitrite and nitrate.Test materialAnalytesChemical:Ash, Carbohydrate, Energy, Fat,Hydroxyproline, Moisture,Nitrate, Nitrite, Phosphate,Protein, Sodium, Salt, Saturates,Mono-unsaturated fats, Polyunsaturated fats, Total Fat, TotalTrans Fatty Acids.Meat and FishMicrobiological:Enumeration:Coliforms, Enterobacteriaceae,Escherichia coli, Total aerobicmesophilic count.Presence/absence:Listeria species, Listeriamonocytogenes, Salmonellaspecies.Note: Test materials and analytes may be added or removed, pleasesee current application form.Email: customerservices@lgcpt.comWebsite: www.lgcpt.com17

FoodPT schemesQMS - Food microbiology schemeThe Quality in Microbiology Scheme (QMS) is aPT scheme intended for use by microbiologistsworking in the food and dairy industries.Food testing is an essential element of the‘Hazard Analysis Critical Control Point’ (HACCP)process in food production as it verifies thecontrols are working at the critical points inmanufacturing. Failures leading to food poisoningoutbreaks can have a devastating effect onreputation, brand value and ultimately profits.Failures which do not lead to an outbreak alsocan have an effect, leading to reduced shelf lifeand wastage. Over time can result in productdefects – affecting customer confidence in theproduct.Microorganisms may be reference strainssourced from national culture collections, ormay be fully characterised wild strains.Atypical strains may occasionally be includedin test materials. Atypical strains can occur in‘real’ foods and could be undetected ormis-identified. It is therefore a realistic challengeto occasionally include atypical strains within aPT scheme.The full range and availability of test materialsin QMS is determined on an annual basis andfurther details can be found in the QMSapplication form and scheme description.Test materialAnalytesLaboratories carrying out such microbiologicaltesting need to be able to demonstrate that theyare producing accurate and meaningful results.This can be done by conducting a comprehensivequality assurance programme, which includesregular participation in a suitable proficiencytesting (PT) scheme.The QMS scheme provides simulated food testmaterials in a choice of matrices that includesoatmeal and skimmed milk powder. The rangeof organisms offered for testing covers all thosethat are likely to be found in food and dairy,from spoilage organisms, pathogens, indicatororganisms and normal background flora.Oatmeal and SkimmedMilk PowderEnumeration:Aerobic psychrotrophs, Bacilluscereus, Coagulase positivestaphylococci, Coliforms,Clostridum perfringens,Escherichia coli,Enterobacteriaceae, Enterococci,Listeria monocytogenes, Lacticacid bacteria, Listeria species,Mesophilic aerobic spores,Thermophilic aerobic spores,Mesophilic anaerobic spores,Sulphite-reducing bacteria.Yeast/Mould,Osmophilic Yeast/Mould.Pseudomonas species,Salmonella species,Thermotolerant coliforms.Total aerobic mesophilic count,Total anaerobic mesophilic count.Presence/absence:Campylobacter species*,Clostridium species, Cronobacterspecies, Escherichia coli,Escherichia coli O157,Listeria species,Listeria monocytogenes,Salmonella species,Vibrio parahaemolyticus,Vibrio species,Yersinia enterocolitica.* Lyophilised vial formatNote: Test materials and analytes may be added or removed, pleasesee current application form.18 Tel: +44 (0)161 762 2500 Fax: +44 (0)161 762 2501

• AquacheckWater chemistry scheme• CONTESTContaminated land scheme• STACKSStack emissions scheme• QWASWater microbiology schemeWater andenvironmentPT schemes

Water and environment PT scheme selectorScheme Scheme year Tests Test material matrixAquacheck April - March Chemical, ecotoxicology, Clean waters and wasteWater chemistry physical and waters, agricultural soils andscheme radiochemical sewage sludge.Water andenvironmentPT schemesCONTEST April - March Chemical and physical Soil extracts, soil materialsContaminated landand standard solutions.schemeSTACKS September - August Chemical and physical Stack / impinger solutionsStack emissionsand filters*.schemeQWAS January - December Microbiological Waters (Various e.g. bathing,Water microbiologyenvironmental, mineral,schemepotable, process, recreational,sea, surface, waste) andsimulated effluent sludge.*Please note that the filter test materials are currently not included in our scope of accreditation.“How often do I need to participate?The frequency that a laboratory needs to participate in proficiency testingdepends on a wide range of factors specific to each individual laboratory.Therefore every individual laboratory may have a different need, which is whyour schemes provide flexible participation, although some do have a minimumparticipation level. Third parties, such as regulatory bodies, may recommendminimum levels of participation. To gain the benefit from trend analysis,participation in a minimum of four rounds over a scheme year is recommended.”20 Tel: +44 (0)161 762 2500 Fax: +44 (0)161 762 2501

Analyte group Number of distributions See pageper scheme yearInorganic, organic and metal analytes in a range of water Twenty 22types. Determination of radiochemical and ecotoxicologicalparameters.Inorganics, organic and metal analytes measured in soil, Five 26leachates and solutions from contaminated land.Inorganic and metal analytes routinely measured in Four 28impinger solutions and filters containing heavy metals.Water andenvironmentPT schemesRoutine microbiological testing, complex pathogen Ten 29detection and indicator organisms.0001Email: customerservices@lgcpt.comWebsite: www.lgcpt.com21

Water andenvironmentPT schemesAquacheck - Water chemistry schemeEverybody depends on water; it is vital insustaining all natural systems on and under theearth’s surface. Water sources require regularanalyses to determine their safety and suitabilityfor a variety of uses. The water we drink mustbe good in quality and quantity so that it is freefrom harmful chemicals and microorganismsin order to guarantee wellbeing. The waterdischarged by local waste water treatmentplants and industry must be monitored toensure strict compliance with environmentalguidelines. Process waters must be kept cleanfrom contaminants to ensure product quality.As water sources can change, regular testing isadvised. The types of analysis could vary fromsimple field testing for a single analyte to complexlaboratory based analysis.The Aquacheck scheme provides test materialsfor the analysis of organic and inorganicchemicals in clean waters; waste waters andeffluents; agricultural soils; sewage sludge;ecotoxicology, radiochemistry and specialdistributions. Different test materials are providedfor different analytical groups: major inorganiccompounds; metals; phenols; organochlorinepesticides and many others. Participants mayselect any number or combination of groupsto suit their analytical requirements. Testparameters within the groups are continuallyreviewed to ensure they meet current laboratorytesting and regulatory requirements.Test materials are distributed frequentlythroughout the scheme year. The use of naturalmatrices ensures that matrix effects are fullyrepresented. Where it is not possible to usenatural matrices, the Aquacheck scheme useseither a synthetic matrix or a simulated materialwhich represents some of the matrix effectslikely to occur in real samples. In this wayparticipation in Aquacheck enables laboratoriesto monitor and improve the quality of theirroutine analytical measurements and providesindependent evidence of laboratory performancefor both laboratory management and customers.It also allows laboratories to demonstrate toregulators and accreditation bodies the validityof their analytical measurements.The full range and availability of test materialsin Aquacheck is determined on an annual basisand further details can be found in the Aquacheckapplication form and scheme description.22 Tel: +44 (0)161 762 2500 Fax: +44 (0)161 762 2501

Test materialAnalytesTest materialAnalytesSample 1HMajor Inorganic Components(in Hard Water)Sample 1SMajor Inorganic Components(in Soft Water)Alkalinity, Barium, Calcium,Chloride, Conductivity (20 o C),Fluoride, Kjeldahl Nitrogen,Magnesium, Nitrogen, Potassium,Sodium, Sulfate, Total Hardness,Total Phosphorus.Alkalinity, Barium, Calcium,Chloride, Conductivity (20 o C),Fluoride, Kjeldahl Nitrogen,Magnesium, Nitrogen, Potassium,Sodium, Sulfate, Total Hardness,Total Phosphorus.Sample 1AChloride, Conductivity (20 o C),Major Ions in Higher Salinity Magnesium, pH at 20-25 o C,Potable WaterSodium, Sulfate, Total DissolvedSolids (180 o C), Total OrganicCarbon (TOC).Sample 2HNutrients and Others(in Hard Water)Sample 2SNutrients and Others(in Soft Water)Ammonia, Colour, Free Cyanide,Nitrate, Nitrite, PermanganateIndex (PI), pH at 20-25 o C, Silicate,Soluble Reactive Phosphorus,Total Cyanide, Total DissolvedSolids (180 o C), Total OxidisedNitrogen (TON).Ammonia, Colour, Free Cyanide,Nitrate, Nitrite, PermanganateIndex (PI), pH at 20-25 o C, Silicate,Soluble Reactive Phosphorus,Total Cyanide, Total DissolvedSolids (180 o C), Total OxidisedNitrogen (TON).Sample 5CChromium (VI) in Clean WaterSample 5GToxic Metals in Groundwater(Preserved in 0.5% Nitric Acid)Sample 6AHaloforms and ChlorinatedSolventsSample 6BPhenolsSample 6CBenzene, Toluene and XylenesSample 7AOrganochlorine PesticidesChromium (VI).Antimony, Arsenic, Beryllium,Cadmium, Chromium, Cobalt,Lead, Mercury, Molybdenum,Nickel, Selenium, Tin, Vanadium.Bromodichloromethane,Bromoform, Carbon Tetrachloride,Chloroform, Dibromochloromethane,Tetrachloroethene, Trichloroethene,1,2-Dichloroethane.Pentachlorophenol, Phenol,2-Chlorophenol, 4-Chlorophenol,2,4-Dichlorophenol,2,4,6-Trichlorophenol.Benzene, Ethylbenzene,m-+ p-Xylene, m-Xylene, o-Xylene,p-Xylene, Styrene, Toluene, TotalXylene.Aldrin, Alpha Endosulphan, AlphaHexachlorocyclohexane, BetaEndosulphan, BetaHexachlorocyclohexane, DeltaHexachlorocyclohexane, DieldrinEndrin, Hepatachlor,Hepatachlorepoxide,Hexachlorobenzene, Lindane(Gamma HCH), o,p-DDT, p,p’-DDD,p,p’-DDE, p,p’-DDT,Pentachlorobenzene, Trifluralin.Water andenvironmentPT schemesSample 2ApH in Poorly Buffered WatersSample 3Non-Specific AnalytesSample 3AInorganic Disinfection By-productsSample 3BFree ChlorineSample 4Metals(Preserved in 0.5% Nitric Acid)Sample 4GMetals in Groundwater(Preserved in 0.5% Nitric Acid)Sample 5Toxic Metals(Preserved in 0.5% Nitric Acid)Sample 5AMetals for Hydride Generation(Preserved in 0.5%Hydrochloric Acid)Sample 5BEQS Metals(Preserved in 0.5% Nitric Acid)pH at 20-25 o C – LowpH at 20-25 o C – HighBOD (5 day), COD, SuspendedSolids, Methylene Blue ActiveSubstances (MBAS), DissolvedOrganic Carbon, Turbidity.Bromate, Bromide, Chlorate,Chlorite.Free Chlorine.Aluminium, Barium, Boron,Copper, Iron, Lithium, Manganese,Silver, Strontium, Zinc.Aluminium, Barium, Boron,Copper, Iron, Lithium, Manganese,Silver, Strontium, Zinc.Antimony, Arsenic, Beryllium,Cadmium, Chromium, Cobalt,Lead, Mercury, Molybdenum,Nickel, Selenium, Tin, Vanadium.Antimony, Arsenic, Selenium, Tin.Cadmium, Copper, Lead, Mercury,Nickel, Total Chronium, Vanadium,Zinc.Sample 7BChlorinated SolventsCarbon Tetrachloride, Chloroform,Hexachlorobutadiene,Tetrachloroethene, Trichloroethene,1,2-Dichloroethane,1,1,1-Trichloroethane,1,2,3-Trichlorobenzene,1,2,4-Trichlorobenzene,1,3,5-Trichlorobenzene.Sample 7CSpiking solution 7C1Polycyclic Aromatic Hydrocarbons Benzo(a)pyrene,(2 Spikes) Benzo(b)fluoranthene,Benzo(ghi)perylene,Benzo(k)fluoranthene,Fluoranthene,Indeno(1,2,3-cd)pyrene.Spiking solution 7C2Acenaphthene, Acenaphthylene,Anthracene, Benz(a)anthracene,Chrysene, Dibenz(ah)anthracene,Fluorene, Naphthalene, Perylene,Phenanthrene, Pyrene.Sample 7D PCB (28), PCB (52), PCB (101),Polychlorinated Biphenyls PCB (118), PCB (138),PCB (153), PCB (180).Sample 8 Spiking solution 8(1)Triazines, Urons and Acid Atrazine, Bentazone, Chlortoluron,Herbicides (2 Spikes)Diuron, Isoproturon, Linuron,MCPA, MCPB, Mecoprop,Propazine, Simazine, 2,4 D.Spiking solution 8(2)Glyphosate.Email: customerservices@lgcpt.comWebsite: www.lgcpt.com23

Test materialAnalytesTest materialAnalytesSample 8BHerbicides (2 Spikes)Spiking solution 8B(1)Bromoxynil, Ioxynil, Monuron,Propyzamide.Sample 17BTotal Phenol, Cyanide andSulphateCyanide, Sulphate, Total Phenol.Water andenvironmentPT schemesSample 9Organophosphorus PesticidesSample 10Nutrients and Other AnalytesSample 11Non-Specific AnalytesSample 12Metals(Preserved in 0.5% Nitric Acid)Sample 12CChromium (VI) in Waste WaterSample 13Sewage Sludge Inorganicsand Specific ElementsSpiking solution 8B(2)Dicamba, Dichlorprop, Trichlopyr,2,4-DB.Azinphos-ethyl, Azinphos-methyl,Chlorfenvinphos, Chloropyrifos,Diazinon, Dichlorvos, Fenitrothion,Fenthion, Malathion, Mevinphos,Parathion-ethyl,Parathion-methyl.Ammonia, Chloride, Free Cyanide,Kjeldahl Nitrogen, Nitrate, Nitrite,Silicate, Soluble ReactivePhosphorus, Total Cyanide, TotalNitrogen, Total Oxidised Nitrogen(TON), Total Phosphorus.BOD (5 day), COD, Dissolved/TotalOrganic Carbon, Methylene BlueActive Substances (MBAS),Suspended Solids.Aluminium, Arsenic, Cadmium,Chromium, Copper, Iron,Lead, Manganese, Mercury,Nickel, Selenium, Zinc.Chromium (VI).Arsenic, Cadmium, Chromium,Cobalt, Copper, Fluoride, Iron,Lead, Manganese, Mercury,Molybdenum, Nickel, Selenium,Total Boron, Total Nitrogen, TotalPhosphorus, Total Potassium,Vanadium, Zinc.Sample 17CMetals(Preserved in 0.5% Nitric Acid)Sample 17DAmmonia, Phosphate andNitrogenSample 18AHaloforms and ChlorinatedSolventsSample 18BPhenolsSample 18CBenzene, Toluene and XylenesSample 19AOrganochlorine PesticidesAluminium, Antimony, Arsenic,Barium, Boron, Cadmium,Chromium, Cobalt, Copper, Iron,Lead, Manganese, Mercury, Nickel,Selenium, Silver, Tin, Vanadium,Zinc.Ammonia, Soluble ReactivePhosphorus, Total Phosphorus,Total Nitrogen.Bromodichloromethane, Bromoform,Carbon Tetrachloride, Chloroform,Dibromochloromethane,Tetrachloroethene,Trichloroethene, 1,2-Dichloroethane.Pentachlorophenol, Phenol,2-Chlorophenol, 4-Chlorophenol,2,4-Dichlorophenol,2,4,6-Trichlorophenol.Benzene, Ethylbenzene,m-+ p-Xylene, m-Xylene, o-Xylene,p-Xylene, Styrene, Toluene,Total Xylene.Aldrin, Alpha endosulphan,Alpha Hexachlorocyclohexane(HCH), Beta endosulphan, BetaHexachlorocyclohexane (HCH),Delta Hexachlorocyclohexane(HCH), Dieldrin, Endrin, Heptachlor,Heptachlor epoxide,Hexachlorobenzene, Lindane(Gamma HCH), o,p-DDT, p,p’-DDT,p,p’-DDE, p,p’-DDD,Pentachlorobenzene, Trifluralin.Sample 14Agricultural Soil Inorganicsand Specific ElementsSample 16Compositional Analysisof Sewage SludgeSample 17AMajor Waste Water AnalytesArsenic, Cadmium, CarbonateContent, Chromium, Colbalt,Conductivity (20ºC), Copper, Drymatter (105±5ºC), ExtractablePhosphorus (Olsen), Extractionof Magnesium, Extraction ofPotassium, Extraction of Sodium,Fluoride, Iron, Lead, Loss onignition (550±25ºC), Manganese,Mercury, Molybdenum, Nickel,Organic Carbon Content(Modified Walkley Black),pH at 20-25ºC (2.5:1 water:soil),Selenium, Total Boron, TotalNitrogen, Total Phosphorus, TotalPotassium, Vanadium, WaterExtractable Boron, Zinc.Calcium, Loss on ignition (500±5ºC)Magnesium, pH at 20-25ºC, TotalSolids (105±5ºC).Conductivity (20 o C), Non FilterableCOD, pH at 20-25ºC, SettledChemical Oxygen Demand (COD),Suspended Solids, Total COD,Total Dissolved Solids (180 o C).Sample 19BChlorinated SolventsCarbon Tetrachloride, Chloroform,Hexachlorobutadiene,Tetrachloroethene, Trichloroethene,1,2-Dichloroethane,1,2,3-Trichlorobenzene,1,2,4-Trichlorobenzene,1,3,5-Trichlorobenzene,1,1,1-Trichloroethane.Sample 19CSpiking solution 19C1Polyaromatic Hydrocarbons Benz(a)pyrene,(2 Spikes) Benzo(b)fluoranthene,Benzo(ghi)perylene,Benzo(k)fluoranthene,Fluoranthene,Indeno(1,2,3-cd)pyrene.Spiking solution 19C2Acenaphthene, Acenaphthylene,Anthracene, Benz(a)anthracene,Chrysene, Dibenz(ah)anthracene,Fluorene, Naphthalene, Perylene,Phenanthrene, Pyrene.Sample 19D PCB (28), PCB (52), PCB (101),Polychlorinated Biphenyls (PCBs) PCB (118), PCB (138), PCB (153),PCB (180).24 Tel: +44 (0)161 762 2500 Fax: +44 (0)161 762 2501

Test materialAnalytesTest materialAnalytesSample 20 Spiking solution 20(1)Triazines, Urons and Acid Atrazine, Bentazone, Chlortoluron,Herbicides (2 Spikes)Diuron, Isoproturon, Linuron,MCPA, MCPB, Mecoprop,Propazine, Simazine, 2,4-D.Sample 21Organophosphorus PesticidesSample 22Qualitative Organics by GCMSSample 22AQualitative Organics by Purgeand Trap GCMSSpiking solution 20(2)Glyphosate.Azinphos-ethyl, Azinphos-methyl,Chlorfenvinphos, Chlorpyrifos,Diazinon, Dichlorvos, Fenitrothion,Fenthion, Malathion, Mevinphos,Parathion-ethyl, Parathion-methyl.Ten organic analytes are providedfor qualitative identification. Thissample is designed to test theability of laboratories to identifyorganic compounds via GCMSanalysis.Participants are provided with asolution containing ten organiccompounds. The test requires thatparticipants identify the tencompounds present. Resultsreturned will be identified assatisfactory or unsatisfactory.Participants are also providedwith a solvent blank.The choice of the ten organiccompounds is designed to avoidthe formation of reactionby-products.Six organic analytes are providedfor qualitative identification. Thissample is designed to test theability of laboratories to identifyorganic compounds via purge andtrap GCMS analysis.Participants are provided with asolution containing six organiccompounds. The test requires thatparticipants identify the sixcompounds present. Resultsreturned will be identified assatisfactory or unsatisfactory.Participants are also provided witha solvent blank.The choice of the six organiccompounds is designed to avoidthe formation of reactionby-products.Sample 25Qualitative Determination inClean WaterSample 26PFOS and PFOASample 27AOX in WastewaterSample 30Gross Alpha and Gross Betain Clean WaterSample 31Aqueous Tritium in Clean WaterSample 50EcotoxicologyThe intent of this sample is to testthe ability of laboratories to detectand identify an unknowncontaminant in surface/potablewaters. This sample is designed forlaboratories which may be involvedin investigating potentiallycontaminated potable or surfacewaters and tests both the extractionand identification stages ofinvestigations.Participants are provided with atwo litre water sample and one ormore ‘indicators’ of a potentialproblem, e.g. water is discolouredor has an oily sheen.Participants are asked to identifythe contaminating substance(s).Results returned will be identifiedas satisfactory or unsatisfactory.Perfluorooctanoic acid,Perflurooctanesulfonic acid.Adsorbable organically boundhalogens.Gross Alpha as 241 Americium,Gross Alpha as 239 Plutonium,Gross Beta as 137 Caesium,Gross Beta as 40 Potassium,Gross Beta as 90 Strontium.Aqueous Tritium.Daphnia magna 24hr EC50,Daphnia magna 48hr EC50,Freshwater algae growth inhibitiontest – biomass reduction(Pseudokirschneriella subcapitata),Marine algae growth inhibition test(Skeletonema costatum), 15 minuteluminescent bacteria IC50 tests,Other 30 minute luminescentbacteria IC50 tests, Oyster 24 hourembryo-larval development test(Crassostrea gigas), Tisbebattagliai 48 hour mortality test,Vibrio fischeri 30 minute IC50(ISO 11348-3), Vibrio fischeri30 minute IC50 (ISO 11348-2).Note: Test materials and analytes may be added or removed, pleasesee current application form.Water andenvironmentPT schemesSample 23Mineral Oil in WaterSample 24Oil and Grease in WaterTotal Hydrocarbons by GC Analysis,Total Hydrocarbons by GravimetricAnalysis, Total Hydrocarbons by IRAnalysis, Volume of sampleprovided.Total Oil and Grease,Volume of sample provided.Email: customerservices@lgcpt.comWebsite: www.lgcpt.com25

CONTEST - Contaminated land schemeTest materialAnalytesWater andenvironmentPT schemesLand contamination can pose both environmentaland human health risks. The main causes ofcontamination are direct discharge of industrialwastes, domestic pollution, over-usage ofpesticides, oil and fuel dumping, leaching ofwastes from landfills and leaking undergroundstorage tanks which corrode over time releasingtoxic substances into previously clean soils.The CONTEST scheme has been exclusivelydeveloped for contaminated soils and the primaryaim is to enable laboratories performing theanalysis of soils to monitor their performance andcompare it with that of their peers. CONTESTwill also enable participants to demonstrate tocustomers, regulators and accreditation bodiesthe validity of their analytical measurements.Participation in a proficiency testing schemefor soil, such as CONTEST, is a requirementof the UK Environment Agency’s MonitoringCertification Scheme (MCERTS) “PerformanceStandard for Laboratories Undertaking ChemicalTesting of Soil”.The CONTEST scheme includes acomprehensive range of analytes of relevanceto the testing of soils. The test materials areavailable as standard solutions, soil extractsand soil materials for the analysis of metals,inorganic contaminants (chlorides, cyanides,fluorides, sulphites), organics (BTEX, chlorinatedhydrocarbons, PAH’s, PCB’s, phenols, TPH) andsoil leachates.The full range and availability of test materialsin CONTEST is determined on an annual basisand further details can be found in the CONTESTapplication form and scheme description.Polyaromatic Hydrocarbons(PAHs):Soil and Standard SolutionAcenaphthene, Acenaphthylene,Anthracene, Benz(a)anthracene,Benz(a)pyrene,Benzo(b)fluoranthene,Benzo(ghi)perylene,Benzo(k)fluoranthene, Chrysene,Dibenz(ah)anthracene,Fluoranthene, Fluorene,Indeno(123-cd)pyrene,Naphthalene, Phenanthrene,Pyrene, Total PAH.Polychlorobiphenyls (PCBs): PCB (28), PCB (52), PCB (101),Soil and Standard Solution PCB (118), PCB (138), PCB (153),PCB (180).Total Petroleum Hydrocarbons(TPH):Soil and Standard SolutionTotal TPH (C 10 -C 40 inclusive),TPH Aliphatic C 10 -C 12 (inclusive),TPH Aliphatic C 13 -C 16 (inclusive),TPH Aliphatic C 17 -C 21 (inclusive),TPH Aliphatic C 23 -C 35 (inclusive),TPH Aliphatic C 36 -C 40 (inclusive),TPH Aromatic EC 10 -EC 12 (inclusive),TPH Aromatic EC 13 -EC 16 (inclusive),TPH Aromatic EC 17 -EC 21 (inclusive),TPH Aromatic EC 22 -EC 35 (inclusive),TPH Aromatic EC 36 -EC 40 (inclusive).Total Petroleum Hydrocarbons TPH Aliphatic ≤C 6 ,(TPH): Volatile range:TPH Aliphatic C 7 -C 8 (inclusive),Standard SolutionTPH Aliphatic C 9 -C 10 (inclusive),TPH Aromatic C 6 (inclusive),TPH Aromatic C 7 -C 8 (inclusive),TPH Aromatic C 9 -C 10 (inclusive).Chlorinated Hydrocarbons:Standard SolutionBTEX:Standard SolutionPhenols:Soil and Standard SolutionsSemi-Volatile OrganicCompounds: (SVOC)Standard SolutionMiscellaneous:SoilChlorobenzenes, Dichloromethane,Hexachloro-1,3-butadiene,Tetrachloromethane,Trichloroethene, Trichloromethane,Vinyl Chloride,1,1,1,2-Tetrachloroethane,1,1,1-Trichloroethane,1,2-Dichloroethane,1,2-Dichloroethene,2-Chlorotoluene.Benzene, Ethylbenzene,m- + p-Xylenes, o-Xylene, Toluene.Cresols, Phenol, XylenolsTotal Phenols (sum of phenol,cresols and xylenols, individuallyquantified).Distillable Phenolic Substances,Total Monohydric Phenols.SVOCs (Qualitative identification).Elemental Sulfur.Total Organic Carbon.26 Tel: +44 (0)161 762 2500 Fax: +44 (0)161 762 2501

Test materialMetals:Soil, Acid Extract of Soil andStandard SolutionTest materialLeachable contaminants:SoilTest materialInorganic contaminants:SoilStandard SolutionAnalytesAntimony, Arsenic, Barium, Beryllium,Cadmium, Calcium, Chromium,Chromium (VI), Cobalt, Copper,Iron, Lead, Manganese, Mercury,Molybdenum, Nickel, Selenium,Thallium, Tin, Vanadium, Zinc.AnalytesAntimony, Ammonia, Arsenic,Barium, Boron, Cadmium, Calcium,Chloride, Chromium, Chromium (VI),COD, Conductivity (20°C), Copper,Fluoride, Free Cyanide, Iron, Lead,Magnesium, Mercury, Molybdenum,Nickel, Nitrate, pH, Phenol Index,Phosphate, Potassium, Selenium,Sodium, Sulfate, Thiocyanate, Tin,TOC/DOC, Total Cyanide, Zinc.AnalytesAmmonia, Complex Cyanide, DryMatter, Easily liberated Sulfide,Free Cyanide, Loss on Ignition,pH, Thiocyanate, Total Cyanide,Total Sulfate, Water Soluble(boron, chloride, fluoride, sulfate).Easily liberated Sulfide,Thiocyanate, Total Cyanide, TotalFluoride, Total Sulfate, WaterSoluble Boron.Water andenvironmentPT schemesNote: Test materials and analytes may be added or removed, pleasesee current application form.Email: customerservices@lgcpt.comWebsite: www.lgcpt.com27

STACKS - Stack emissions schemeThe STACKS scheme has been designed forperiodic manual measurement of industrialstacks by the analysis of simulated impingersolutions for a range of common pollutants.Water andenvironmentPT schemesModern (and not so modern) combustion andindustrial processes produce a range of airpollutants that have been shown to have, orare suspected of possessing, harmful effects onhuman health and the environment. Pollutantsnot only have local impact, but can also travellong distances, chemically reacting in theatmosphere to produce secondary pollutantssuch as acid rain or the much publiciseddamage to the ozone layer.Industrial companies are required by regulatoryauthorities and environmental agencies tomeasure emissions from their chimney stacks.Monitoring of these emissions helps plants tomanage their impact on the environment. Bymeeting the set limits, companies can avoidheavy fines and adverse publicity.EU legislation (Directive 96/61/EC and itscodified version 2008/1/EC) dictates that theemissions from stationary stacks such aschimney stacks are monitored in order to preventor reduce emissions and that measures aretaken to monitor such emissions.Test materialImpinger SolutionsAnalytesAmmonia, Antimony, Arsenic,Cadmium, Chromium, Cobalt,Copper, Hydrogen Chloride(Hydrogen Chloride and HCl),Hydrogen Fluoride, Lead,Manganese, Mercury, Nickel,Sulfur Dioxide, Thallium,Vanadium.Participation in a proficiency testing scheme forstack emission monitoring is a requirement forlaboratories accredited to carry out this analysisunder the UK Environment Agency’s MonitoringCertification Scheme (MCERTS) or if the testingforms part of a laboratory’s scope ofaccreditation to ISO/IEC 17025.The test materials used in the scheme will reflectthe types of materials generally encounteredby participants. The preparation and analysisof the test parameters are all based on therelevant EN Standards or equivalent, and arecontinually reviewed to ensure they meet currentlaboratory testing and regulatory requirements.The full range and availability of test materialsin STACKS is determined on an annual basisand further details can be found in the STACKSapplication form and scheme description.*Metals on filters (Quartz or PTFE)Antimony, Arsenic, Cadmium,Chromium, Cobalt, Copper, Lead,Manganese, Nickel, Thallium,Vanadium.Note: Test materials and analytes may be added or removed, pleasesee current application form. *Please note that the filter test materialis currently not included in our scope of accreditation.28 Tel: +44 (0)161 762 2500 Fax: +44 (0)161 762 2501

QWAS - Water microbiology schemeThe QWAS scheme is solely intended formicrobiological analysis of waters, effluents andsludges and helps microbiologists demonstratereliable results when conducting analysis on awide range of water test materials. QWAS canhelp laboratories to meet the requirements ofthe laboratory standard ISO/IEC 17025, whichstates that analytical results must be monitoredusing external quality assessment, and theEU Directive 98/83/EC dealing with the qualityof water for human consumption.The test materials are provided in a stable,lyophilised format and QWAS participants canchoose any combination of simulated potable,process and surface/wastewater materials,or an environmental water test material forLegionella testing. There is also an effluentsludge test material to allow participants tomonitor treated sewage sludge which is usedas an agricultural fertilizer. A sea water testmaterial for bathing purpose as monitored by theEU for awarding ‘blue flag’ status for beaches isalso available.Microorganisms may be reference strainssourced from national culture collections, ormay be fully characterised wild strains. Whenreconstituted, test material quantities vary,depending upon the microbiologicalspecifications for the organism under test.Atypical strains may occasionally be includedin test materials. Atypical strains can occur in‘real’ samples and could be undetected ormis-identified. It therefore represents a realisticchallenge to occasionally include atypical strainswithin a PT scheme.For laboratories that perform the analysis ofwater, participation in a relevant proficiencytesting scheme can provide confidence thatresults of these analyses are meaningful andaccurate which, in turn, helps to ensure thesafety of water.The full range and availability of test materialsin QWAS is determined on an annual basis andfurther details can be found in the QWASapplication form and scheme description.Test materialWaters (Various e.g. bathing,environmental, mineral, potable,process, recreational, sea, surface,waste) and Simulated EffluentSludge.AnalytesEnumeration:Clostridium perfringens, Coliforms,Enterococci (faecal streptococci),Escherichia coli, Faecal coliforms,Legionella species,Pseudomonas aeruginosa,Pseudomonas species,Staphylococcus aureus,Total coliforms, Yeast and Mould.Total aerobic mesophilic count,Total aerobic mesophilic countat 22°C,Total aerobic mesophilic countat 37°C.Presence/absence:Sulphite – reducing clostridia,Salmonella species.Note: Test materials and analytes may be added or removed, pleasesee current application form.Water andenvironmentPT schemesEmail: customerservices@lgcpt.comWebsite: www.lgcpt.com29

Quality control materialsPost-report PT test materials availableAt the end of PT rounds, material is available for up to 3 months to theparticipants in order to investigate any issues with the analytical process.This is often required by accreditation bodies where there is no obviousnon-analytical error (such as a transcription error) that could explain theoccurrence of a poor score in a PT round.Value quality control materialsFor many of our schemes there is ample stock available after the reporthas been issued, and these are ideal for use as quality control materialswithin the day-to-day laboratory programme. For many schemes, thesamples are commercially produced materials with long shelf-lives,making them the perfect solution for many of your QC requirements.Proven qualityThe assigned value for the PT test materials is supported by the analysisof many laboratories (in some cases hundreds) and statistical dataprocessing, to ensure the reported values are reliable*.Benefits of PT test materials for quality control• Proven values, fully documented in the PT report• Excellent value for money• Ideal for method validation• May be used to help establish measurement uncertainty• Perfect for process control between PT rounds• Excellent training tool for new staff• Saves time preparing in-house controls• Helps validate internally prepared materials• Underpins your internal quality proceduresFor further information contact: LGC Standards Proficiency TestingEuropa Business Park, Barcroft Street, Bury, Lancashire BL9 5BT, UKTel: +44 (0)161 762 2500 Fax: +44 (0)161 762 2501Email: customerservices@lgcpt.com Web: www.lgcpt.com*Some materials use reference values and these are determined bydifferent processes, depending upon the scheme. Please check thespecific scheme report for the material required.

• QBSBeverage scheme• BAPSBrewing analytes scheme• DAPSDistillers’ analytes scheme• MAPSMalt analytes scheme• SUPSSugar schemeBeveragePT schemes

Beverage PT scheme selectorScheme Scheme year Tests Test material matrixQBS January - December Chemical and Carbonated drink, carbonatedBeverage scheme microbiological drink (degassed), dilutable /ready to drink fruit juice,mineral water and soft drinkBAPS January - December Chemical, microbiological Ales, lagers and stoutsBrewing analytesand sensoryschemeBeveragePT schemesDAPS April - March Chemical Distilled spirits, fermented worts,Distillers’ analytescider, wines and fortified wines,schemeliqueurs and cream liqueurs, andready to drink alcoholic beveragesMAPS January - December Chemical and physical Brewing/distilling malted barley,Malt analytes schemebarley and malting cerealsSUPS January - December Chemical and Cane or beet sugar andSugar scheme microbiological molasses“My results have not been included in the reportcan I calculate my own performance score?To calculate your performance score please visit www.lgcpt.com/portal”Select ‘help’ from the menu and download the z score calculator.32 Tel: +44 (0)161 762 2500 Fax: +44 (0)161 762 2501

Analyte group Number of distributions See pageper scheme yearChemical tests of relevance to the beverage industries for Four 34quality checks and complex parameters.Microorganisms of relevance to beverage products,including pathogens and indicator organisms.Chemical parameters of relevance to the brewing industry Twelve - Chemistry 35for quality checks and complex analysis.Six - MicrobiologyBrewery spoilage organisms.Twelve - SensorySensory parameters of relevance to the brewing industryoffering assessments in aroma and taste evaluation.Chemical tests of relevance for alcoholic beverages and Four 36intermediate process samples.BeveragePT schemesChemical and physical tests of relevance to the malting Twelve 37industries for quality checks and complex analysisincluding mycotoxin analysis.Chemical tests of relevance to the sugar processing and Twelve 38and food industries. Microorganisms of relevance tosugar products, including pathogens and indicatororganisms.0001Email: customerservices@lgcpt.comWebsite: www.lgcpt.com33

QBS - Beverage schemeThe QBS scheme is specifically tailored forchemists and microbiologists working in the fruitjuice, mineral water and soft drinks industries(including carbonated drinks). The schemecan also be of value to other laboratories thatneed to test for general food additives, such assweeteners and preservatives.Fruit juice and beverage products are regularlytested in order to ensure that they are ofacceptable chemical and microbiological qualityfor consumption.BeveragePT schemesChemical test materials are ready-to-use liquidmatrices, covering product quality andcompositional values. This enables laboratoriesto assess performance and demonstratecompliance with the relevant compositionalstandards and the statutory limits governingthe use of additives. It also shows that the testsused to monitor other ingredients are reliable,essential to maintain the organoleptic propertiesof the beverage so vital to product quality.Test materialCarbonated drink, Carbonateddrink (degassed), Dilutable/Readyto drink, Fruit juice and Soft drink.AnalytesChemical:Acesulfame K, Acidity (as CitricAcid Monohydrate), Ascorbic Acid(Vitamin C), Aspartame, BenzoicAcid, Brix, Caffeine, Carbon Dioxide,Free and Total Sulfur Dioxide,Fructose, Glucose, Headspace Air,Saccharin (as Amide), Sorbic Acid(as Free Acid), Sucrose, pH.Heavy Metals and Minerals:Antimony, Cadmium, Calcium,Iron, Lead, Phosphorus,Potassium, Magnesium, Sodium.Chemical analytes available for testing withinthe QBS scheme include a comprehensiverange of quality checks (including brix, titratableacidity, pH) in addition to other more complexparameters which range from common additives(e.g. preservatives and sweeteners) to heavymetals and minerals and miscellaneous additivessuch as caffeine and vitamin C.Test materials for microbiological testing coverthe main spoilage and indicator organisms andare provided as lyophilised material with achoice of diluent (fruit juice, soft drink ormineral water) and a test material for analysisusing filtration method to meet the laboratory’sparticular requirements.Fruit juice, Mineral water andSoft drinkMicrobiological:Enumeration:Enterococci, Escherichia coli,Lactic acid bacteria,Pseudomonas aeruginosa,Total aerobic mesophilic count,Total aerobic mesophilic countat 22°C,Total aerobic mesophilic countat 37°C,Yeast and Mould.Presence/absence:Escherichia coli.Microbiological Filtration:Enumeration:Lactic acid bacteria,Total aerobicmesophilic count, Yeast and Mould.Presence/absence:Escherichia coli.Note: Test materials and analytes may be added or removed, pleasesee current application form.The full range and availability of test materialsin QBS is determined on an annual basis andfurther details can be found in the QBSapplication form and scheme description.34 Tel: +44 (0)161 762 2500 Fax: +44 (0)161 762 2501

BAPS - Brewing analytes schemeThe BAPS scheme is designed to promotequality in the measurement of a range ofchemical, microbiological and sensory analytesin real beer matrices. It is jointly run by LGCStandards and Campden BRI.Analytes available are divided into five samples:Chemical (Samples 1, 2 and 3), Microbiological(Sample 4), Sensory (Sample 5).For Samples 1, 2, 3, 4 and 5, participants mayselect the samples and rounds they wish toreceive. As part of a comprehensive qualityassurance programme, and to gain the benefitof trend analysis, participation in all the roundsover a year is recommended.Sample 1 is designed to provide participantswith test materials that encompass a range ofkey brewing analytes that are routinelyanalysed in the brewing industry.Test materialSample 1Chemical analysisSample 2Chemical analysisSample 3Chemical analysisAnalytesAlcohol by Volume, Bitterness,Carbon Dioxide, Carbon Dioxide(Pressure) corrected for foreigngas, Carbon Dioxide (Pressure)uncorrected for foreign gas,Colour @ 430 nm, Haze at 0°C,Haze at 20°C, Original Extract,Original Gravity, pH,Present Gravity, Refractive Index,Sulfur Dioxide.Acetaldehyde, Calcium, Chloride,Copper, Dimethyl Disulfide,Dimethyl Sulfide, Energy value(kcal), Energy value (kJ), EthylAcetate, Ethyl Hexanoate, FAN,Free Diacetyl, Free 2,3-Pentanedione, Free VDK as Diacetyl,Glucose, HRV – NIBEM, HRV –Rudin, Hydrogen Sulfide, Iron,Iso-α-acids, Iso-Amyl Acetate,Iso-Butanol, Magnesium, Maltose,Maltotetraose, Maltotriose,Methanethiol, Methylthioacetate,Nitrate, n-Propanol,Phosphate,Potassium, Sodium, Sulfate,Tetra-iso-α-acids, TotalCarbohydrate, Total Polyphenols,TSN, 2+3 Methyl Butanol,2-Methyl Butanol, 3-Methyl Butanol.Bitterness, Colour at 430nm,Colour at 530nm, Free 2,3-Pentanedione, Free Diacetyl, FreeVDK as Diacetyl, Iso-α-acids,Tetra-iso-α-acids.Sample 2 is designed to provide participantswith test materials that encompass a range ofanalytes that complement the routine analysisprovided by Sample 1.Sample 3 is a test material of beer with highbitterness content and/or a high colour value.Sample 4 is designed to provide participantswith microbiological test materials containingorganisms typically encountered in the brewingindustry.Sample 5 test materials are provided to allowparticipants to evaluate various aroma and tastecharacteristics found in the brewing industry.Campden BRI will undertake suitability testingusing their sensory panel and provide anexpert profile.The full range and availability of test materialsin BAPS is determined on an annual basis andfurther details can be found in the BAPSapplication form and scheme description.BeveragePT schemesSample 4Microbiological analysisWild Yeasts (anaerobic andaerobic), Brewing Yeasts (ale andlager), Lactic Acid bacteria, AceticAcid bacteria and other organisms.Sample 5Sensory analysisAroma:Alcoholic/Solvent, Burnt, Cereal,Fruity/Estery, Hop, Malty, Sulfur,Sweet, Toffee and others.Taste:Alcoholic/Solvent, Astringent,Bitter, Burnt, Cereal, Fruity/Estery,Hop, Linger, Malty, Sour, Sulfur,Sweet, Toffee and others.Note: Test materials and analytes may be added or removed, pleasesee current application form.Email: customerservices@lgcpt.comWebsite: www.lgcpt.com35

DAPS - Distillers’ analytes schemeAs well as distilled beverages, DAPS coversanalysis of a wide range of alcoholic beveragesand intermediate process samples. The rangeof beverages includes fermented worts, scotchwhisky, clear and dark spirits, liqueurs, ciders,wines and ready to drink alcoholic beverages(also known as ‘lifestyle’ drinks). DAPS does notinclude any test materials of beer – there is aseparate scheme for beer, BAPS (see page 35).BeveragePT schemesThere are five different groups available, with acomprehensive range of test parameters, assummarised in the table.One of the key tests performed within DAPSis the measurement of alcoholic strength. Dutyis normally paid on the basis of the alcohol inthe beverage, and so it is a key performanceindicator for the product and tax authorities.By monitoring laboratory performance in thiskey area, laboratories can help ensure thatoverpayment is not made thus saving money.Underpayment, which could lead to financialpenalties, can also be avoided.Furthermore, many of the key parameters inDAPS are related to flavour compounds withinthe beverage and so, by ensuring consistentanalysis of these parameters, beverageproducers may improve consistency of productacross batches. Such parameters includeacetaldehyde, furfural, 2+3 methyl butanols,ethyl acetate and acetic acid.The full range and availability of test materialsin DAPS is determined on an annual basis andfurther details can be found in the DAPSapplication form and scheme description.Test materialGroup ADistillers’ Fermented WortGroup BDistilled SpiritsGroup CCiderGroup DWines and Fortified WinesGroup ELiqueur, Cream Liqueur and Readyto drink alcoholic beveragesAnalytesAlcohol by volume, Final Gravity,pH, Original and Residual Gravity,Residual Fermentable Sugars(total amount of glucose, maltoseand maltotriose).Acetic Acid, Apparent AlcoholicStrength, Actual AlcoholicStrength, Acetal, Acetaldehyde,Calcium, Citric Acid, Colour,Copper, Ethyl Acetate, EthylCarbamate, Furfural, Fructose,Glucose, Glycerol, Haze, Iron,Iso-Amyl Acetate, Iso-Butanol,Linalool (gin only) Magnesium,Maltose, Methanol, n-Butanol,NDMA, n-Propanol, pH,Potassium, Propylene Glycol,Sodium, Sucrose, Terpinene-4-ol(gin only), Total Acidity, VolatileAcidity, 2 + 3-Methyl butanols,2-Methyl butanol, 3-Methyl butanol.Actual Alcoholic Strength, CarbonDioxide, Colour, Haze, pH,Specific Gravity, Total Acidity,Total Sulfur Dioxide, Volatile Acidity.Actual Alcoholic Strength, AscorbicAcid, Citric Acid, Colour @ 420nm,Colour @ 520nm, Colour @ 620nm,Copper, Free Sulfur Dioxide,Fructose, Glucose, Iron, LacticAcid, Malic Acid, pH, Sorbic Acid,Specific Gravity, Total Acidity, TotalSulfur Dioxide, Volatile Acidity.Actual Alcoholic Strength, Acetal,Acetaldehyde, Ascorbic Acid,Benzoic Acid, Carbon Dioxide,Citric Acid, Ethyl Acetate, Furfural,Glycerol, Iso Amyl Acetate,Iso-Butanol, Methanol, n-Butanol,n-Propanol, pH, Propylene Glycol,Residue, Sorbic Acid, SpecificGravity, Total Acidity, Total Sugars,Volatile Acidity, 2+3 Methylbutanols,2-Methylbutanol, 3-Methylbutanol.Note: Test materials and analytes may be added or removed, pleasesee current application form.36 Tel: +44 (0)161 762 2500 Fax: +44 (0)161 762 2501

MAPS - Malt analytes schemeThe MAPS scheme covers test materials fromthe full range of malted barley, barley and othermalting cereals used for brewing and distilling.These test materials are analysed for a widerange of analytes, using EBC (European BrewingConvention) and IBD (Institute of Brewing andDistilling) methods, as well as a number ofother physical and chemical methods. All testmaterials are sourced from commercially-tradedmalting barleys.Test materialMaltAnalytesChemical:Alpha Amylase, Cold WaterExtract, Diastatic Power, DMSP,DPWK, EBC Fraction IV (

BeveragePT schemesSUPS - Sugar schemeThe Sugar Proficiency Testing Scheme (SUPS)is suitable for all laboratories concerned withthe quality of sugar, particularly manufacturersof sugar and sugar products and the soft drinksindustry.All matrices are sourced from commercialmanufacturers as cane or beet sugar andmolasses. Participants receive test materialsquarterly for monthly analysis and reportingof results.SUPS is the only scheme designed to providesugar analysts with information relevant to themethods of analysis used to define the qualityof sugar. It is based on the use of specifiedmethods available in the ICUMSA (InternationalCommission for Uniform Methods of SugarAnalysis) Methods Book that have undergoneinternational validation.Test materialSugar is traded all around the world and it istherefore essential that those laboratoriesanalysing sugar for trade purposes know exactlyhow they perform when carrying out theseanalyses. SUPS provides data which helpswith identification of any performance issueswith respect to the methods used and providesevidence of competence for individuallaboratories.The full range and availability of test materialsin SUPS is determined on an annual basis andfurther details can be found in the SUPSapplication form and scheme description.Cane or Beet SugarAnalytesChemical:Arsenic, Ash, Colour, Copper,Iron, Lead, Reducing Sugars,Reflectance Grade, Sulfur Dioxide,Turbidity.Sediment:Sediment (insoluble),MolassesLyophilised MaterialChemical:Dry Substance, pH, Sucrose,Reducing Sugars.Microbiological:Total aerobic mesophilic count,Osmophilic Yeast and Mould,Yeast and Mould.Note: Test materials and analytes may be added or removed, pleasesee current application form.38 Tel: +44 (0)161 762 2500 Fax: +44 (0)161 762 2501

• PACQSParticle analysis andcharacterisation quality scheme• PHARMASSUREPharmaceutical scheme• PHYTASPhytochemical scheme• QMISMicrobiology investigationscheme• QUARTZForensic blood toxicologyscheme• TOYTESTToy safety schemeOtherPT schemes

Other PT scheme selectorScheme Scheme year Tests Test material matrixPACQS* September - August Physical Particulate materialsParticle analysis andcharacterisation schemePHARMASSURE April - March Chemical and Pharmaceutical productsPharmaceutical microbiological, and standard solutionsschemeinstrumental techniquesPHYTAS* September - August Chemical BotanicalPhytochemical schemeQMIS January - December Microbiological Lyophilised microorganismsMicrobiologyinvestigation schemeQUARTZ September - August Chemical Blood and standard solutionsForensic bloodtoxicology schemeOtherPT schemesTOYTEST January - December Chemical assessment Toys and standard solutionsToy safety schemeagainst standards*Please note that the PACQS and PHYTAS schemes and alcohol test material are currently not included in our scope of accreditation.“How can I receive advice and feedback?Communication with participants will be carried out through scheme-relateddocumentation, e-mails, letters, newsletters, memos, fax, or through LGCStandards local offices. Open meetings may also be organised and allinterested parties invited to attend.”40 Tel: +44 (0)161 762 2500 Fax: +44 (0)161 762 2501

Analyte group Number of distributions See pageper scheme yearParticle size analysis by wet and dry laser diffraction. Four 44Basic/advanced chemical analysis, microbiological analysis Four 45and sterility testing.Comprehensive range of botanical products for Four 46identification and quantification of active ingredients.Food and water pathogens, indicator and spoilage Two 47organisms.Drug identification, quantification and alcohol Four 48quantification.Metals analysis, toy safety assessment exercises and Four 49physical measurement assessments.OtherPT schemes0001Email: customerservices@lgcpt.comWebsite: www.lgcpt.com41

PACQS - Particle analysis andcharacterisation quality schemeThe PACQS scheme, for users of laserdiffraction particle size analysers, is designedto give invaluable feedback on the accuracy ofparticle sizing measurements. These are criticalto product quality in a wide range of industries,from pharmaceuticals to paints, cosmetics toceramics and more. PACQS can be a powerfultraining tool for laboratory and productionpersonnel carrying out the tests as they will getdirect feedback on their analytical performance.The scheme is managed in accordance withinternational standard for laser diffractionparticle analysis (ISO 13320-1). Participantshave the added value of being able to contactthe appropriate instrument manufacturers, whocontribute to the independent advisory group,to seek advice with respect to their performancein the scheme.OtherPT schemesThe full range and availability of test materialsin PACQS is determined on an annual basisand further details can be found in the PACQSapplication form and scheme description.Test materialVarious materials, providinga range of sizes andphysical/optical propertiesAnalytesParticle diameter statisticsdetermined by Laser diffractiontechniques:D 10 value,D 50 value,D 90 value,D 4 , 3 (volume weighed mean).Note: Test materials and analytes may be added or removed, pleasesee current application form. PACQS is currently not included in ourscope of accreditation.42 Tel: +44 (0)161 762 2500 Fax: +44 (0)161 762 2501

PHARMASSURE - Pharmaceutical schemePHARMASSURE has been specifically designedto meet the needs of the pharmaceutical industry.The reduction of risks associated with themanufacture of pharmaceutical products is theprimary objective of the pharmaceutical qualityassurance laboratory. There is an abundanceof regulations, biological standards, validationprocedures and other tools available to the qualitycontrol professional to achieve this aim. In mostcases however, the use of these tools is aimedat assessing the product or the environment inwhich it was produced. The introduction ofproficiency testing offers a valuable independenttool for the ongoing assessment of the testingprocedures, methods employed, laboratory staffresponsible for the analysis and subsequentinterpretation of results.Test materialSample 1Basic chemical analysisSample 2Advanced chemical analysisSample 3Microbiological identificationAnalytesAcid/Base Titration, Density,Melting Point, Other Basic Titration(actual analytes dependant ontest), pH, Refractive Index.Advanced Titration (e.g.Potentiometric, Non-Aqueous),Flame Spectroscopy, FTIR,HPLC, GC, IR, Polarimetry, UV,Viscosity.A low inoculum level test materialfor enumeration by membranefiltration and identification.The regulations surrounding the manufacture ofpharmaceutical products and the laboratorieswhich test them are wide ranging, andcompliance with the requirements of GMP/GLPare aimed at ensuring the safety and efficacy ofall pharmaceutical preparations. Similarly, theannual revalidation of methods is an importanttool in understanding and ensuring that a testingmethod is working properly. However, these toolsare largely internal operations. Regularparticipation in a PT scheme, provides anexternal independent tool for monitoring theeffectiveness of methods employed in anylaboratory, without the work involved in a fullmethod revalidation. The test materials coverchemical and microbiological analysis. Thesterility test is ideally suited to ongoingassessment and provides staff involved in theanalysis and in interpretation of results with atool to ensure that low level contaminationevents which occur particularly infrequently –and often not at all over the course of a testingyear – are identified and the results correctlyinterpreted.The full range and availability of test materialsin PHARMASSURE is determined on an annualbasis and further details can be found in thePHARMASSURE application form and schemedescription.OtherPT schemesSample 4Microbiological enumerationSample 5Sterility testingA high inoculum level test materialfor enumeration of Total aerobicmesophilic bacteria,Staphylococcus aureus,Escherichia coli, Yeasts andMoulds.A set of 5 test materials each tobe tested for sterility.Note: Test materials and analytes may be added or removed, pleasesee current application form.Email: customerservices@lgcpt.comWebsite: www.lgcpt.com43

PHYTAS - Phytochemical TestingAssurance SchemePHYTAS has been developed in response todemands from the phytochemical industry.In recent times there has been a huge amountof media attention and popular interest inmedicinal plants and the chemicals they contain.These plant materials are highly valued for therole they can potentially play in promoting andsustaining human health. Plants have evolvedto produce an array of secondary compounds,many of which have been used in traditionalmedicines and remedies for hundreds of years.The most common classes of ‘active’ compoundfound in medicinal plants are alkaloids,terpenoids, phenols and glycosides, though therange of compounds is by no means limited tothese four classes. As extracts of living matter,the quality and potency of botanical products issubject to variation, giving rise to uncertaintyamongst consumers, analysts and regulatorsalike.The analysis of such a wide range ofcompounds, in what is by definition an incrediblydiverse range of matrices, is not alwaysstraightforward and there is little standardisationof methodologies from laboratory to laboratory.One of the major concerns in a relatively newand broad field of analysis is this lack ofstandardised methods and even definition ofthe ‘required’ components for analysis.Proficiency testing is an important tool in theimprovement of analytical performance inscientific disciplines such as this and bystandardising the quality of analysis, one ofthe variables is removed – helping to achieveconsistency in the quality of the finishedproduct for the consumer.The full range and availability of test materialsin PHYTAS is determined on an annual basisand further details can be found in PHYTASapplication form and scheme description.OtherPT schemesTest materialBotanicalAnalytesIdentification and quantificationof a range of active ingredients.Note: Test materials and analytes may be added or removed, pleasesee current application form. PHYTAS is currently not included in ourscope of accreditation.44 Tel: +44 (0)161 762 2500 Fax: +44 (0)161 762 2501

QMIS - Microbiology investigation schemeThe Quality in Microbiology InvestigationScheme (QMIS) is designed to work alongsidethe QMS scheme (see page 18).QMIS differs from the normal schemes in thatthe microbiologist must carry out specifiedlaboratory techniques and apply furtherinvestigative measures in order to identify anunknown organism provided for analysis. Ittherefore assesses the ability to carry outfundamental microbiological techniques, andfrom the results of these, determine the finalidentity of the isolate.Each test material is provided with briefinformation about its origin, along with narrativedetails, from which the microbiologist mustdeduce the necessary tests (in addition to thoseprescribed in the accompanying information,such as Gram stain, catalase, oxidase, etc.)required to achieve the correct identificationof the organism.Test materialFood and WaterAnalytesFood:Unknown pathogen foridentification with scenario.Food:Unknown routine organism foridentification with scenario.Water:Unknown isolate for identificationwith scenario.QMIS organisms are selected from ourcomprehensive range of microorganisms ofrelevance to food and water analysis and arepresented in a lyophilised format to includepathogens, indicator organisms and spoilageorganisms.Note: Test materials and analytes may be added or removed, pleasesee current application form.OtherPT schemesThe full range and availability of test materialsin QMIS is determined on an annual basis andfurther details can be found in the QMISapplication form and scheme description.Email: customerservices@lgcpt.comWebsite: www.lgcpt.com45

QUARTZ - Forensic blood toxicology schemeQUARTZ is a blood toxicology scheme and isdesigned for laboratories undertaking analysis ofdrugs in post-mortem and other blood samples fortoxicological purposes, particularly in a forensiccontext. Test materials are prepared by spikingpre-screened human blood, which are assessedfor homogeneity prior to despatch.The analytes to be determined in each round arefrom a list, compiled by the Advisory Group, ofnon-prescription, prescription and controlleddrugs, as well as other toxins, which reflect whatparticipants are likely to encounter in forensiccasework.Test materialGroup AMore frequently determined drugsAnalytes6MAM (MACM), Amfetamine,Amisulpride, Amitriptyline,Benzoylecgonine, Buprenorphine,Carbamazepine, Chlordiazepoxide,Chlorpromazine, Citalopram,Clomipramine, Clozapine, Cocaine,Codeine, Cyclizine,Desmethyldiazepam, Diazepam,Diclofenac, Dihydrocodeine,Diphenhydramine, Dosulepin,Fentanyl, Fluoxetine, Ibuprofen,Imipramine, Ketamine, Lamotrigine,MDA, MDMA, Methadone,Methamphetamine, Midazolam,Mirtazepine, Morphine, Olanzapine,Oxazepam, Oxycodone,Paracetamol, Paroxetine, Pethidine,Phenytoin, Procyclidine,Promethazine, Propoxyphene,Quetiapine, Risperidone, Salicylate,Sertraline, Temazepam,THC,THC-COOH,Tramadol, Venlafaxine,Zolpidem, Zopiclone.OtherPT schemesThe drugs used are subdivided into two groups:Group A those drugs that are more frequentlydetermined by participants.Group B those drugs that may be lessfrequently determined by participants.Test material will contain between 0 and 4 drugs andup to three samples are provided in each round.Alcohol is a major cause of road casualties anddeaths and as penalties for drink-driving aresevere it is essential the accuracy of analysiscan be proven in the legal case. The test materialcomprises 10ml vial of whole blood containingalcohol for analysis. The full range and availabilityof test materials in QUARTZ is determined on anannual basis and further details can be found in theQUARTZ application form and scheme description.Group BLess frequently determined drugsTest materialDrug IdentificationDrug QuantificationAmlodipine, Amobarbital, Atenolol,Benzyl piperazine (BZP),Butobarbital, Clobazam,Clomethiazole, Clonazepam,Dextromoramide, Dipipanone,Gabapentin, Loprazolam,Lormetazepam, Mefenamic Acid,Methylphenidate, Naltrexone,Pentobarbital, Phenelzine,Propranolol, Secobarbital,Sildenafil, Thioridazine,Trazadone, Zaleplon.Note: Metabolites of the above substances may also be added.AnalytesParticipants will be asked toidentify the drug(s) only. The testmaterial will always (if positive)contain one drug from Group A.Up to three other drugs may bein the test material from eitherGroup A and/or Group B.Participants will be told the identity,or generic classification, of thedrug(s), and asked to quantify theconcentration. They will also beasked to give an interpretation ofthe results in respect to a casestudy provided. The test materialwill always contain a drug fromGroup A. Any other drugs presentwill be from Group A and/or Group B.Drug Quantification*Alcohol QuantificationStandard solutions containingdrugs for the evaluation ofinstrumentation.Participants will be required toquantify the alcohol concentrationby their usual methodsNote: Test materials and analytes may be added or removed, pleasesee current application form. *Please note the Alcohol test material iscurrently not included in our scope of accreditation46 Tel: +44 (0)161 762 2500 Fax: +44 (0)161 762 2501

TOYTEST - Toy safety schemePlay is a vital part of child-development and toyshelp at every stage from babies to school-agechildren. Toys are safer than ever before, butwe should remain cautious about often hiddenhazards of toys. We rely on manufacturers,suppliers and toy legislation to help keep ourchildren safe and at the same time preventproduct recalls and protect brand value.With an international reputation as a leadingindependent PT supplier, LGC StandardsTOYTEST scheme allows laboratories involved inthe safety testing of toys to monitor their analysisagainst the current European and Americanstandards. Participation in TOYTEST will enablelaboratories performing tests on toy productsto monitor their performance and compare withthat of their peers.Test materialTest usually a toy material ortoy partA paper exercise relating to theinterpretation and inspection of toysA standard solution containingmetals at levels similar to atypical extractSection of material and/orstandard solutionDried paint flakesSection of plastic materialAnalytesEN71-1: Test material for analysisand assessment against standard.EN71-2: Test material forflammability testing.EN71-3: Test material and Standardsolution provided for metal analysis.EN71-7: Information provided forassessment against standard.EN71-8: Information provided forassessment against standard.EN71-9: Test material for analysisand/or information provided forassessment against standard.ASTM F963: Test materialassessment against standard.Azo dyes.ASTM: Total Lead.Phthalates.Test materials are assessed against therequirements of EN71 (European Standard)and ASTM (American Society for Testing andMaterials F963) toy safety standards.A comprehensive range of test materials will beincluded over the scheme year to be assessedagainst the regulations; however, not all parts ofthe standards will be assessed over a schemeyear. Participants will be given clear instructionsas to which parts of the standards are to beassessed.Note: Test materials and analytes may be added or removed, pleasesee current application form.OtherPT schemesTest materials will also be provided for analyticaltesting, such as paint flakes for lead analysis,and standard solutions for heavy metal analysis.Further tests may be added at the discretion ofthe Advisory Group.The full range and availability of test materialsin TOYTEST is determined on an annual basisand further details can be found in the TOYTESTapplication form and scheme description.Email: customerservices@lgcpt.comWebsite: www.lgcpt.com47

Genuine ATCC culturesand bioproducts for theEuropean pharmaceuticalcommunityLGC Standards is the exclusive distributor of ATCC ®cultures and bioproducts in Europe. We are committedto providing authentic, high-quality ATCC cultures tosupport quality control and research activities acrossthe pharmaceutical industry.With a local stock of all key quality control strains anda wide selection of other cultures, LGC Standards is ableto offer:●●●●Faster delivery of ATCC productsExpert technical supportExpedited handling of special forms and paperworkto meet import regulationsReduced shipping costsFor further information or to receive any one of ourcatalogues, please contact your local office or visitour website.Quality control strainsGenomic DNAAnimal and plant virusesCell lines and hybridomascDNA clones, vectorsand librariesMicrobial media andsupplementsCell culture media, seraand reagentsCryopreservation productswww.lgcstandards.com/atcc

• Special bespoke microbiologytest materials• Closed customised PT schemes• Other PT services• Product development• Reference materials• Training and education• SummaryOther services

Special bespoke microbiology test materialsIn the unlikely event that our standard schemesdo not fulfil your company’s requirements, wewould be happy to discuss any special requestsyou may have, subject to a minimum order value.Special test materialrequest received bycustomer servicesPlease down load the special request form fromthe www.lgcpt.com website and send thecompleted form to: customerservices@lgcpt.comDISCUSSION PHASENewrequestDiscuss technical feasibilityand costs with laboratoryRepeatorderEnter request onto specialorder databaseChangesrequiredPrint confirmation formand fax or emailto the customerDatabase notifieslaboratory toprepare test materialsConfirm order received inspecial order databaseCustomer checksconfirmation and approvesor makes changesApproved(signed &order no.)Preparation ofworking stockQuality control ofworking stockPreparation ofbulk stock or vialsOther servicesPRODUCTION PHASEPack, despatch andinvoice test materialsLevelsout of spec. *(delaypossible)Label stock,produce certificateLevelsin spec.Levelsin spec.Quality control of bulkstock or vialsLevelsout of spec. *(delaypossible)Test materials receivedby customerSpecial Notes: The production phase does not start until an approved (signed, dated and with purchase order number where applicable) confirmationform has been received by LGC Standards. *Products out of specification are more likely with special test materials as defined levels are oftenrequested. As we are dealing with live organisms, the final levels cannot always be accurately predicted, resulting in further work needing to be carriedout and resulting delay in completion of the manufacturing process.50 Tel: +44 (0)161 762 2500 Fax: +44 (0)161 762 2501

Closed customised PT schemesReference materialsIn addition to the regular schemes,LGC Standards is able to offer customised orclosed proficiency testing schemes tailored to aspecific organisation’s requirements. We operatesuch schemes on behalf of some of the largestcompanies in the world and we are happy todiscuss your requirements in this area. Pleasenote that a minimum level of business isrequired before a scheme can be setup.For further details contact:customerservices@lgcpt.comOther PT services• Advice and consultancy for potential PTproviders;• Consultancy for PT providers in theimplementation of appropriate qualitysystems;• Training courses for PT participants andtheir customers;• Prominent role in the development of policyand guidance for proficiency testing byrepresenting the UK on a number of keyinternational committees.Product developmentLGC Standards combines expertise in analyticalsciences, and experience in internationalstandards, to offer laboratories throughoutEurope the ideal solution to sourcing high qualitycertified reference materials.Our expert technical support staff unrivalledknowledge in the use of certified referencematerials across a broad range of analyticalsectors.As the leading global supplier of referencematerials, LGC Standards offers:• Partnership with the world’s key producersof reference materials;• Expert local customer service and technicalsupport;• Supply of all major pharmacopeia andreference materials;• Assistance in finding the right materials tosuit your need;• Over 25,000 reference materials in our range.For further information or to receive any one ofour catalogues, please contact your local officeor visit our website: www.lgcstandards.comLGC Standards Proficiency Testing is continuallystriving to improve current products and tointroduce new products and PT schemes whereappropriate. New products may be introducedinitially by offering them on a trial basis toparticipants. It will be made clear to participantswhen they are participating in a trial.Other servicesIf you have a requirement for a new analyte,test material matrix, or a whole new schemeplease contact:www.customerservices@lgcpt.comEmail: customerservices@lgcpt.comWebsite: www.lgcpt.com51

Training and educationA range of training courses is available fromLGC Standards to help laboratory managersand analysts demonstrate competence in, andkeep abreast of, quality assurance issues andpractices. LGC Standards analytical qualitytraining programme includes:• Achieving traceability in chemical testing;• Using proficiency testing in the analyticallaboratory;• Method validation;• Quality systems in testing laboratories;• Statistics for analytical chemists;• Further statistical tools for analyticalchemists;• Principle and practice of measurementuncertainty in chemical testing laboratories;• Evaluating measurement uncertainty forchemical testing laboratories.The majority of the courses are run inTeddington, South West London. In additionLGC Standards can provide training for groupsof staff at your own site, where the coursescan be customised to meet your exact needs.SummaryProficiency testing is widely used across manyindustries as an integral part of the qualitycontrol and risk reduction process. Similarlyparticipation in these types of programmesprovides the analytical laboratory with theability to assess performance on an ongoingbasis and benchmark that performance againstother laboratories while maintaining anonymity.Confidentiality of participant results is anabsolute requirement of proficiency testing andis assured for all participants. The ongoingassessment of performance using proficiencytesting allows the identification of areas fortraining and improvement and may also assistwith audit processes.Proficiency testing is only one of the many toolsthat can bring about improvements in yourlaboratory’s quality system. Other ways mayinclude the use of quality control materialsand reference materials. LGC Standards canoffer our market leading products from ourcatalogues covering a wide range of sectorsincluding food, environmental, pharmaceutical,clinical and industrial.Other servicesTo find out more about the training coursesand seminars available and to make an on-linebooking visit our website: www.lgc.co.uk52 Tel: +44 (0)161 762 2500 Fax: +44 (0)161 762 2501

Frequently askedquestions

Frequently askedquestionsFrequently asked questionsQ: Which international standards are relevantto PT?A: All the PT schemes within LGC StandardsProficiency Testing are operated in accordancewith the international standard ISO/IEC 17043.The statistical analysis undertaken is inaccordance with the international standardISO 13528. LGC Standards Proficiency Testingis accredited by the United Kingdom AccreditationService for the provision of proficiency testingschemes, currently against ISO/IEC Guide 43.1and ILAC G13. A copy of our current scope ofaccreditation is available on the UKAS website(www.ukas.com).Q: How are your schemes organised?A: The day-to-day operation of each scheme isthe responsibility of LGC Standards ProficiencyTesting. Individual schemes are managed bya team of Scheme Coordinators, to coverreporting, customer service and technicalfunctions. For some schemes, externaladvisors may also be used to provide the fullrange of relevant knowledge and expertiseneeded to operate the scheme effectively.Q: Do you use Advisors and Advisory Groups?A: Yes depending upon the scheme in question.Advisors are selected on the basis of theirtechnical knowledge and experience of theindustry to which the scheme is related.Advisors may be used on an ad-hoc basisand contacted when specific issues need tobe addressed.Alternatively, formal advisory groups may beused. Advisory Groups consist of memberswho may or may not be participants on thescheme but who are experienced in the fieldof testing covered by the PT scheme.The composition and terms of reference ofeach Advisory Group will be agreed on ascheme-by-scheme basis. Membership ofthe Advisory Groups is subject to change butmembers’ names are available on request.Q: Do you run schemes that are jointlymanaged?A: Yes one or two schemes are operated jointlywith a partner organisation. Where schemesare operated jointly, a Management Committeemay be set up to address operational issuesfor the scheme.Q: How do I join a scheme?A: Participants are advised to participate in thescheme(s) that are most appropriate to theirown area of testing. Where necessary, staffat LGC Standards Proficiency Testing canadvise on which scheme is most suitablefor participants.For each scheme, a scheme description andapplication form will be available, containinginformation about the test materials includedin the scheme, and the intended distributiondates. Participants are invited to completethe application form, indicating which testmaterials they wish to receive during thescheme year. If the availability of test materialschanges during the scheme year, participantsare kept fully informed.Once a participant joins a scheme and hasselected the test materials required, aparticipant schedule is provided as confirmation.Q: Can you guarantee my laboratory’sconfidentiality?A: In order to ensure confidentiality, participantsin all schemes are allocated a uniquelaboratory reference number. This numberenables results to be reported withoutdivulging the identities of participantlaboratories. Only LGC Standards ProficiencyTesting and the laboratory itself know thisnumber.Q: How often do I need to participate?A: The frequency that a laboratory needs toparticipate in proficiency testing depends ona wide range of factors specific to eachindividual laboratory, such as, other quality toolsused, the volume of work undertaken and the54 Tel: +44 (0)161 762 2500 Fax: +44 (0)161 762 2501

isk associated to the measurements.Therefore every individual laboratory mayhave a different need, which is why schemesprovided by LGC Standards offer flexibleparticipation, although some do have aminimum participation level. Third partiessuch as regulatory bodies, may recommendminimum levels of participation. To gain thebenefit from trend analysis, participation in aminimum of four rounds over a scheme yearis recommended.Q: What are the fees for participation?A: Fees for participation are reviewed annuallyand the current fees for each scheme areavailable on application. Payment terms aredetailed on the application form and invoice.Participants are advised that delays withpayments may result in test materials andreports being withheld until payment is made.Q: Where do you source your test materials?A: The vast majority of test materials aremanufactured by LGC Standards ProficiencyTesting. Where this is not possible, testmaterials are carefully sourced to meet theneeds of participants. Wherever practical,test materials will be as similar as possibleto those routinely tested by participatinglaboratories. However, in some cases, inorder to achieve the required degree ofhomogeneity and stability, test materials maybe in the form of simulated matrices orconcentrated spiking solutions.The range of test materials will usually bevaried from round to round in order to berealistic and challenging. Details of individualtest material types are available in the relevantscheme description.Q: How are test materials packaged andtransported?A: Test materials are sent in appropriate packagingand under conditions intended to protect thecontents during transit. All test materials aresent using priority courier. Overseascustomers must provide relevant documentsto prevent delay in customs such as importpermits and may be required to pay importduties locally.Once packages have been delivered,LGC Standards Proficiency Testing cannot beheld responsible if they subsequently failto reach the correct personnel or are notstored under the recommended conditions.Participants are asked to check the contentsof packages immediately on receipt and tocontact LGC Standards Proficiency Testingif there are any problems with the conditionof the test materials or accompanyingdocumentation.Q: How is test material stability affected bytime, distance and temperature?A: The test materials are all stable at the statedstorage temperatures for at least the periodof the Proficiency Test round. Studies haveshown there is no significant differencebetween results of test materials tested theday after despatch and those tested on thedeadline date. There is also no evidence thatresults are influenced by different climaticconditions of participating countries.Distance travelled does not affect test materialresults. We have undertaken studies on anumber of our PT test materials comparingthe average result according to distancetravelled, and no correlations have beenfound. Stability consideration is an importantpart of the design and feasibility process fora PT scheme, where transport conditionssuch as temperature, humidity, pressure,exposure to x-rays etc. are taken into account.Q: How do I treat my PT test material?A: It is important for laboratories to understandhow to get the optimum benefit from PTparticipation. To do this, a laboratory mustparticipate in an open and honest fashion,being prepared to, on occasion, beevaluated as unsatisfactory. If PT is toachieve its aims, laboratories need to treatthe PT test materials the same as routineFrequently askedquestionsEmail: customerservices@lgcpt.comWebsite: www.lgcpt.com55

Frequently askedquestionstest materials, and staff must be encouragedto treat them appropriately and learn fromtheir results in a constructive manner.Laboratories will learn very little about thequality of their routine work if the PT testmaterials are given special treatment, suchas carrying out a much higher number ofanalyses, in order to be evaluated assatisfactory. This may in fact compromisethe quality of routine measurements as adisproportionate level of effort is beingexpended for the PT.Q: Do I have to use specific methods toanalyse the test materials?A: Unless otherwise instructed, participantsshould analyse the test materials usingany method that they feel is appropriate.Participants are asked to treat the PT materialin the same way as a routine test material.Participants may be asked to state theirmethod when reporting results. It isimportant that this information is accurateas results are analysed and reportedaccording to the method stated.Q: Do I have to report my results within aspecific timescale?A: Deadlines are specified for the return ofresults, to ensure the timely issue of assignedvalues and reports to participants. For eachscheme a closure date will therefore bespecified. For certain tests there may also bea date specified by which examination of thetest material is recommended to have beencommenced. This is to ensure that sufficienttime is available to complete the test andreport results in time for the deadline date.Q: How should I report my results usingPORTAL?A: For the majority of schemes, results arereturned through our bespoke electronicreporting software, PORTAL. Once you areready to report your results, please go towww.lgcpt.com/portal you will need to login using your Lab ID, Username andPassword. We advise that prior to usingPORTAL you read the user guide which isavailable at www.lgcpt.com/portal select‘help’ from the menu. If you require furtherassistance please contact our support team.Email: support@lgcpt.comTel: +44(0)161 762 2500. For your localLGC Standards office see pages 64 - 65.For some schemes (or parts of a scheme)alternative reporting mechanisms areprovided, details of which will be emailedto participants prior to test materials receipt.It is recommended that results andcalculations are checked thoroughly beforereporting. Results should be reportedclearly, in the format and units detailed inthe scheme description. If calculations areused, unless instructed otherwise, thelaboratory is to report only the finalcalculated result.In general, results of zero should not bereported; results should be reporteddepending upon the detection limit of themethod used, for example, cannot beincluded in the data analysis and thereforecan not be allocated a performance score.Results may be rounded up or down in thereport and may not therefore be identical toyour original reported result. However,performance scores are calculated usingthe unrounded results submitted and so areunaffected by rounding. The effects ofrounding may also mean that occasionallypercentage totals do not add up exactlyto 100%.Part of the challenge of proficiency testingis the ability to perform calculations and56 Tel: +44 (0)161 762 2500 Fax: +44 (0)161 762 2501

transcribe results correctly. LGC StandardsProficiency Testing staff cannot interpret orcalculate results on participants’ behalf.Once submitted and received, results cannot be amended and no changes can bemade after the report has been issued.Q: Can my results be included in the reportif I’ve missed the deadline for reporting?A: Participants are asked to return results bythe given deadline in order to ensure that theirresults are included in the statistical analysisand the scheme report. Results receivedafter the closure date will not be included inthe report.For schemes where a generic report is issued,this is available to all participants subscribingto the round regardless of whether theirresults were submitted or not.practicable and technically feasible theassigned value will be derived throughformulation (or occasionally through the useof a certified reference material) to providemetrological traceability; the associateduncertainty of the value can therefore beestimated. However, in many cases the useof a consensus value is the only practicableand technically feasible approach to use.When the assigned value is determinedfrom the consensus value of participantresults, or from expert laboratories, robuststatistical methods are used for calculationof the consensus value, the estimatedstandard uncertainty and the robuststandard deviation.For qualitative tests, participant results arecompared against the intended result basedon formulation.Q: How do you prevent collusion andfalsification of results?A: It defeats the objective of taking part inproficiency testing if participants are notreturning genuine results. Certain measuresare built into the schemes to try and preventcollusion but, ultimately the responsibilityrests with each participating laboratory tobehave in a professional manner.For schemes where the result is subjectiverather than quantifiable, a model answerproduced by appropriate experts will bepublished in the report.For microbiology test materials, all participantresults are transformed by converting themto log 10 before the statistical analysis isundertaken.Q: How is the assigned value established?A: ISO 13528: ‘‘Statistical methods for use inproficiency testing by interlaboratorycomparisons” sets out how the assignedvalue and performance assessment criteriacan be established and describes theoptions for the various scoring systems.The assigned value is the value selected asbeing the best estimate of the ‘true value’for the parameter under test. The methodused to determine the assigned value mayvary depending upon the particular schemeand test material and is detailed in therelevant scheme description.For quantitative tests, where it is appropriate,Q: How do I evaluate measurementuncertainty?A: The aim when evaluating measurementuncertainty is to combine the effects of allthe errors, that will influence the measurementresult, into a single value. There are manydifferent guides available which provideadvice on evaluating measurementuncertainty. Two specific guides that areinternationally recognised, are:- ISO (BIPM, IEC, IFCC, IUPAC, IUPAP &OIML) "Guide to the Expression ofUncertainty in Measurement"- EURACHEM/CITAC Guide "QuantifyingUncertainty in Analytical Measurement"Frequently askedquestionsEmail: customerservices@lgcpt.comWebsite: www.lgcpt.com57

Frequently askedquestionsFurther information on approaches toevaluating measurement uncertainty mayalso be available from your nationalaccreditation body. For example, UKASM3003 "The expression of uncertainty andconfidence in measurement".The EURACHEM/CITAC Guide can bedownloaded, along with further informationon evaluating measurement uncertainty,from the website www.nmschembio.org.ukQ: Can I use PT data to estimate mymeasurement uncertainty?A: It is possible, but must be regarded as avery rough estimate, and is not an approachaddressed in many guides to evaluatingmeasurement uncertainty. However, twodocuments that do address the use of PTdata are:- NORDTEST Report TR 537 "Handbook forCalculation of Measurement Uncertainty inEnvironmental Laboratories"- ISO/TS 19036 "Microbiology of food andanimal feeding stuffs - Guidelines for theestimation of measurement uncertainty forquantitative determinations"Q: What is the standard deviation forproficiency assessment (SDPA)?A: The SDPA expresses the acceptabledifference between the laboratory result andthe assigned value. An acceptable z-scorerepresents a result that does not deviatefrom the assigned value by more than twicethe SDPA. The method used to determinethe SDPA may vary depending upon theparticular scheme and test material and isdetailed in the relevant scheme description.Q: What standard deviation for proficiencyassessment (SDPA) is used inmicrobiology PT schemes?A: There are many sources of variation inmicrobiological testing and the SDPA used toassess performance therefore needs to befit-for-purpose and take all possible sourcesof variation into account. From experienceand historical data, LGC Standards PT usesa fixed SDPA value of 0.35 log 10 for themajority of microbiological tests.Q: What is the purpose of scoring my result?A: Once the assigned value for the parametersunder test has been established, participantlaboratories are assessed on the differencebetween their result and the assigned value,with this difference being represented by aperformance score called a z-score. Thisprovides a simple and consistent measureof performance which is the key tomonitoring competence and implementingan improvement programme as required.Q: How are z-scores calculated?A: The participant’s result, x, is converted intoa z-score using the following formula;z = (x - X)SDPAWhere: X = the assigned value SDPA =standard deviation for proficiency assessment.For small data sets, there will be increaseduncertainty around the assigned value ifusing consensus values from participants’results. In such cases, z-scores may not beprovided, or may be given for informationonly.The z-score expresses performance inrelation to the assigned value and standarddeviation. A z-score of 2 represents a resultthat is a distance of 2 x SDPA from theassigned value.A fixed value for SDPA is preferable as thisenables z-scores to be compared from roundto round to demonstrate general trends.For each scheme, the value of SDPA andthe method used to derive it is reported inthe scheme description and/or report.58 Tel: +44 (0)161 762 2500 Fax: +44 (0)161 762 2501

Q: How do I interpret my results?A: For quantitative examinations, participantperformance is assessed using the z-score,and the following interpretation is given toresults.|z| ≤ 2.00Satisfactory result2.00 < |z| < 3.00 Questionable result|z| ≥ 3.00Unsatisfactory resultFor qualitative examinations, laboratoriesreporting the assigned result will beconsidered correct.Q: What are the advantages of a z-score?A: • Results can be expressed in a form that isrelatively easy to interpret and understand;• Results can be summarised in graphical ortabular form to depict overall performance;• A performance score allows participants todirectly compare their own result with others;• If consistent statistical values are applied,a performance score enables participantsto monitor and trend their own performanceover time.It is important to interpret any performancescore in the full context of the overall resultsand in the context of a laboratory’s ownquality control measures.of all sources of error, such as gravimetricand volumetric measurements.If u x is ≤ 0.3 x SDPA, then the uncertaintyof the assigned value can be considerednegligible and need not be considered in theinterpretation of results.If u x is > 0.3 x SDPA, then the uncertainty ofthe assigned value is not negligible in relationto the SDPA and so z’- scores (z-prime),which takes into account the standarduncertainty of the assigned value in theircalculation, will be reported in place ofz-scores.Q: How is z’- score (z-prime) calculated?A: A z’- score (z-prime) incorporates thestandard uncertainty of the assigned valueand is calculated as follows:z’ = (x - X)√ SDPA 2 2+ u xWhere x = participant resultX = the assigned valueSDPA = standard deviation forproficiency assessmentu x = standard uncertainty ofthe assigned value XQ: What is the estimated uncertainty of theassigned value?A: The assigned value has a standarduncertainty (u x ) that depends upon themethod used to derive the assigned value.When the assigned value is determined bythe consensus of participants’ results, theestimated standard uncertainty of theassigned value can be calculated by;u x = 1.25 x Robust standard deviation/√ nWhere n = number of resultsWhen the assigned value is determined byformulation, the standard uncertainty isestimated by the combination of uncertaintiesA z’-score is interpreted in exactly the sameway as a z-score, ≤2 is satisfactory, >2 but

Frequently askedquestionsbut those results that are out of the range ofthe assigned value ± 5 x SDPA will beexcluded and the robust mean and standarddeviation will then be recalculated. Theserecalculated values will be used for thestatistical analysis. By removing these‘blunders’ from the dataset any influenceon the summary statistics is completelyremoved. All results, including excludedresults, will be given z-scores.Q: What could be the cause of my poorperformance?A: A single poor result is not indicative of overalllaboratory performance but neither is asingle good result. Ideally, PT results shouldbe monitored over time to detect unusual biasor repeated unsatisfactory results indicatepoor performance.There are many possible reasons for a singleunsatisfactory result including statisticalchance. It is therefore important to interpretthe results from PT schemes within thecontext of an all-round quality assuranceprogramme, including internal quality control,use of validated methods and referencematerials.There are numerous potential causes ofunsatisfactory performance in a PT schemewhich may include analytical andnon-analytical errors:Analytical errors• Calibration / instrument problems;• Extraction / clean-up;• Interferences / matrix effects;• Diagnostic kits / reagents;• Analyst / method performance.Non-analytical errors• Calculation / transcription;• Reporting format / units;• Storage;• Test material defects.Test materials are subjected to rigorous qualitycontrol testing before being distributed toparticipants, and are unlikely to be the causeof a poor z-score.All possible reasons for a poor performanceshould be investigated fully in order to identifythe most likely cause and to enable actionto be taken to prevent recurrence. Repeattest materials are available after everydistribution, but it is most important toinvestigate and understand the reason(s)for the failure, document this fully, and carryout corrective actions before repeating a test.Q: How can I measure my laboratory’sperformance over time?A: You can do this by Trend Analysis. A singleresult simply reflects the performance of thelaboratory on the particular day that the testwas carried out and therefore gives limitedinformation. Frequent participation in PTschemes over time can give greater insightinto long-term performance and can helpidentify where internal bias may be occurring.One of the best methods of summarisingz-scores over time is graphically as this givesa clear overview and is less prone tomisinterpretation than numerical methods.Participants are therefore advised to monitortheir PT results over time. Further informationregarding interpretation and trend analysisof proficiency results is given in the IUPAC“International Harmonised Protocol for theProficiency Testing of Analytical ChemistryLaboratories”, 2006 and ISO 13528.Trend Analysis is an integral part of ourPORTAL software.Q: How can I graphically plot and analysetrends for qualitative results?A: Qualitative results are difficult to depictgraphically as they are not normally allocateda performance score. However for qualitativeresults, a correct result could be allocated a60 Tel: +44 (0)161 762 2500 Fax: +44 (0)161 762 2501

performance score of 0 to represent asatisfactory result. A false positive result canbe represented by a performance score of+ 3, whilst a false negative result can berepresented by a performance score of - 3.If plotted graphically over time, this shouldgive a clear visual indicator of performancein qualitative tests.Q: How will I receive my report?A: Following statistical evaluation of the results,the reports will be available on the websitewithin 4 to 15 working days of round closure(see specific scheme description). Participantswill be emailed a link to the report when it isavailable. The contents of reports vary fromscheme to scheme but include details of thecomposition of test materials, the assignedvalues, and tabular and/or graphicalrepresentations of participants’ results.Paper copies are also available for anadditional charge.Q: My results have not been included in thereport can I calculate my performancez score?A: To calculate your performance score pleasevisit www.lgcpt.com/portal Select ‘help’from the menu and download the z scorecalculatorQ: How can I receive advice and feedback?A: Communication with participants will becarried out through scheme-relateddocumentation, e-mails, letters, newsletters,memos, fax, or through LGC Standardslocal offices. Open meetings may also beorganised and all interested parties invitedto attend.Q: How can I send feedback?A: Comments on any aspect of our productsand services are welcome either by phone,fax, letter, email or by contacting your localLGC Standards office.Q: How do I assess the reproducibilitystandard deviation from the PT report?A: The robust standard deviation provided inthe PT report for a specific method can betaken as an estimate of the reproducibilitystandard deviation for the PT round for thatspecific method.Q: Can I have a report that only includes mygroup laboratories?A: Yes we can produce reports tailored to acustomer’s specific requirement. There willbe an additional charge for administrationand computer programming costs.Copyright to all reports remains with LGCStandards Proficiency Testing but permission isgranted to participants to make copies for theirown internal use, for example for quality controland regulatory purposes. No other copies maybe made without obtaining permission.Email: customerservices@lgcpt.comWebsite: www.lgcpt.com61

ChromaDex phytochemicalsand servicesPhytochemical pure substances●●●●Reference standardsPrimary standardsRare chemicalsProduct librariesContract services●●●●●Contract analysisContract researchProcess developmentIsolationMethod developmentReference materials●●●Botanical reference materials (BRM)Extract reference materials (XRM)Voucher specimensAnalysis kits●●●●Standard kitsHPLC kitsTLC kitsBioluminex© LGC Standards, 1872/SR/0508www.lgcstandards.com

LGC Standardsoffices

LGC Standards Proficiency Testing has dedicated local offices worldwide to help with your needs from placingan order through to specific enquiries. Please see the list below to contact your nearest office.BulgariaIvan GenovLGC Standards”G. M. Dimitrov” Blvd. 52A, office 102,1797 SofiaBulgariaTel: + 359 (0)2 971 4955Fax: + 359 (0)2 971 4955bg@lgcstandards.comCountries served:Bulgaria.Czech RepublicPatricie CarasovaLGC Standards Sp. z o.o., org. slozkaHnevkovskeho 65Brno617 00Tel: +420 543 529 205Fax: +420 543 529 205patricie.carasova@lgcstandards.comCountries served:Czech Republic, Slovak Republic.FranceM. Hervé MohrLGC Standards SARL6, Rue Alfred Kastler,B.P. 8307667123 MOLSHEIMFranceTel: +33 (0)3 88 04 68 83Fax: +33 (0)3 88 04 82 90herve.mohr@lgcstandards.comCountries served:Algeria, Belgium, Benin, Burkina, Burundi,Cameroon, France, Gabun, Ivory Coast,Jordan, Lebanon, Liechtenstein, Luxembourg,Madagascar, Mali, Mauritania, Mauritius,Monaco, Morocco, Rwanda, Senegal, Syria,Tanzania, Tunisia.GermanyLiliane HeinrichsLGC Standards GmbHMercatorstr. 5146485 WeselGermanyTel: +49 281 9887 215Fax: +49 281 9887 259liliane.heinrichs@lgcstandards.comCountries served:Albania, Austria, Bosnia-Herzegovina, Croatia,Cyprus, Egypt, Germany, Greece, Hungary,Iran, Israel, Japan, Korea, Kosovo, Macedonia,Mongolia, Montenegro, Netherlands, People’sRepublic of China, Romania, Serbia, Singapore,Slovenia, Switzerland, Taiwan, Turkey, Vietnam.IndiaM M PrashanthLGC Promochem India Pvt. Ltd.#488-B, 4th Floor, 1st BlockVITC Model Export Bhavan14th cross, 4th PhasePeenya Industrial AreaPeenyaBangaloreIndiaTel: +91 80 6701 2025Fax: +91 80 6701 2046prashanthmm@lgcpromochem.inCountries served:India.ItalyCristina CarpiLGC Standards S.r.lVia Venezia, 23,20099 Sesto San Giovanni MIItalyTel: +39 02 24126 842Fax: +39 02 24126 831cristina.carpi@lgcstandards.comCountries served:Italy.LGC Standardsoffices64 Tel: +44 (0)161 762 2500 Fax: +44 (0)161 762 2501

PolandAgnieszka WojdaLGC Standards Sp.zo.o.ul. M. Konopnickiej 1Dziekanów Leśny05-092 ŁomiankiTel: +48 (0)22 751 31 40Fax: +48 (0)22 751 58 45agnieszka.wojda@lgcstandards.comCountries served:Armenia, Azerbaijan, Belarus, Georgia, KyrgyzRepublic, Kazakhstan, Lithuania, Poland,Tajikistan, Turkmenistan, Ukraine, Uzbekistan.SwedenLarisa MuravevaLGC Standards ABAlbanoliden 5 (4th Floor)P.O. Box 1737SE-501 17 BorasSwedenTel: +46 33209061Fax: +46 33209079larisa.muraveva@lgcstandards.comCountries served:Denmark, Estonia, Finland, Greenland, Iceland,Latvia, Norway, Sweden.RussiaSvetlana BuchakaLGC Standards Sp. z o.o., Representative officeSankt-Peterburgskij prosp. 60A,Petershof, Saint-Petersburg198516 RussiaTel: +7 812 935 1180Fax: +7 812 334 4825ru@lgcstandards.comCountries served:Russia.SpainJuan Galisteo OchaítaLGC Standards S.L.U.C/ Salvador Espriu no 592nd Floor08005 BarcelonaSpainUnited KingdomLGC StandardsEuropa Business ParkBarcroft StreetBuryLancashireBL9 5BTTel: +44 (0) 161 762 2500Fax: +44 (0) 161 762 2501customerservices@lgcpt.comCountries served:Australia, Bangladesh, Bermuda, Botswana,Brunei, Canada, Channel Islands, Ethiopia,Ghana, Indonesia, Ireland, Kenya, Kuwait,Malaysia, Malta, Nepal, New Zealand, Nigeria,Oman, Pakistan, Philippines, Qatar, SaudiArabia, South Africa, Sudan, Tobago, Trinidad,United Arab Emirates, United Kingdom, USA,Yemen, Zambia, Zimbabwe.Tel: 00 34 93 3084181Fax: 00 34 93 3073612juan.galisteo@lgcstandards.comCountries served:Andorra, Argentina, Bolivia, Belize, Brazil, Chile,Columbia, Costa Rica, Ecuador, El Salvador,French Guiana, Guyana, Guatemala, Honduras,Mexico, Nicaragua, Panama, Paraguay, Peru,Portugal, Spain, Suriname, Uruguay, Venezuela.Email: customerservices@lgcpt.com Website: www.lgcpt.com 65LGC Standardsoffices

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