Image Viewing with WebScope - School of Medical Sciences

WebScope OverviewWebScope• A platform–independent digital slide viewer.• Does not require client side software installation.• Can be used from any workstation (Macintosh, Linux, etc.).• Uses any Internet browser that supports Adobe Flash Player.Certain WebScope features are onlyavailable when used with an Aperiosystem in which digital slides are servedby Aperio ImageServer. User permissionare controlled by the role assigned to theuser.Page 2

WebScope ToolsToolbar SummaryIntegrated ColorManagementShow/HideThumbnailAdjustBrightness/ContrastSave AnnotationsNegative PenEllipseImageInformationWebScopeInformationShow/HideNavigationToolbarAnalyzeDigital SlidePanPenRulerRectangleArrowWebScope QuickReferenceShow/HideAnnotationsWindowPage 5

WebScope ToolsNavigation Toolbar SummaryCurrentMagnificationLevelMove IndicatedDirectionHome: fits entiredigital slide to fitwindow.MagnificationSlider barZoom In/OutCreate Link toDigital SlidePage 6

WebScope Basic FeaturesOpen Slide in WebScope• Depending on the Aperio product, the method of opening a digital slide inWebScope differs.• Spectrum and Spectrum Education:• Click the thumbnail image to launch WebScope.If ImageScope is installed, hold downthe W key while you click the thumbnailimage.Page 7

WebScope Basic FeaturesOpen Slide in WebScope• KickStart for Educators:• Access the website served by KickStart.• Click WebScope link under the digital slide to be opened.Page 8

WebScope Digital Slide NavigationNavigation• Panning - use the mouse to left-click and drag the image (this is the fastestmethod).• Use the arrows to move in one direction.• Click anywhere in the thumbnail to move to a new location quickly.The thumbnailimage showsthe entire digitalslide.Page 9

WebScope Digital Slide NavigationChange Magnification• Quickly zoom to max magnification by double-clicking on the image.• Set the magnification to a specific level using the slider icon.• Zoom in and out using the icons.• Rest the view to fit the entire image in the main window using the icon.Page 10

WebScope AnnotationsAnnotations Window• Displays details about annotations that have been drawn on the digital slide.• Use the window to:• Show or hide annotations on main window.• Place text labels on the annotations.Click the icon to show or• View analysis results.hide the annotations• Change the color of the drawing.window.Layers of annotationsdisplay in different colors.Layer Region displaysdata for each region withinthe Layer.Page 11

WebScope AnnotationsAnnotation Drawing Tools• Annotations can define a region of interest and measure an object on the digitalslide.• To use a tool, select tool, left-click and move across the image.Pen *Negative Pen*(excludes area)Ruler (measures in microns)Rectangle*EllipseArrow*These annotation toolscan be used to define regions toperform or exclude Image Analysis.Tip: Right click to selectan annotation tool.Page 12

WebScope AnnotationsLayers and Attributes• Each group of annotations can exist on a separate layer.• Organize information by pathologist or department.• Each layer is given a separate color to easily differentiate one from another.• Each layer can have one or more attributes.• Attributes can be text stored with the layer or analysis data.Show/hide layersChange ColorAdd LayerDelete Selected LayerDelete All LayersLayer Attributes displayscombined image analysisresults for all regions.Page 13

WebScope Image AnalysisAnalyze a Digital Slide in WebScope• Use the drawing tools to define an area of interest (unless doing whole slideanalysis).• Click on the main toolbar to open the Algorithm job window.• Select a macro, region of analysis and if a markup image is to be generated and clickAnalyze.A statusmessageinforms thejob status.Page 14

WebScope Image AnalysisAnalysis Results• Quantitative results are available in a new layer in the Annotations window.Page 15

WebScope Image AnalysisMarkup Image• If requested, a markup image will be displayed with the digital slide.For information on themeaning of the differentpseudo-colors used inthe markup, see theuser’s guide for theparticular algorithm youare using.Page 16