KPL ELISA Brochure

Phosphatase Substrates for ELISAReserveAP TMConjugatesImprove your assayperformance even furtherwith high potencyReserveAP TM AntibodiesComparison of AP colorimetric substrates for ELISAHuman IgG was serially diluted in an ELISA plate and detected with goat anti-human IgG labeledwith alkaline phosphatase. KPL’s substrates, FirePhos, BluePhos, pNPP and a competitor pNPPwere added to the plate. The reactions were stopped with APstop Solution and read at the recommendedwavelength for each substrate. The results indicate that FirePhos and BluePhos produce asignificantly higher signal-to-noise relative to pNPP.Ordering InformationCatalog# Description SizeFirePhos TM AP Microwell Substrate50-87-10 FirePhos Microwell Phosphatase Substrate System 50 mL50-87-20 FirePhos Microwell Phosphatase Substrate System 200 mL50-87-30 FirePhos Microwell Phosphatase Substrate System 600 mL50-87-40 FirePhos Microwell Phosphatase Substrate System 2.7 LKPL’s new ReserveAP TM AlkalinePhosphatase-labeled Conjugatesexhibit high potency and consistentperformance in immunoassays.These conjugates are theresult of advances in our conjugationtechnology and offer highersignal and lower background thanour current line of alkaline phosphataseconjugates while meetingthe same standards for stabilityand reproducibility. They are idealfor immunoassays that requirehigh detection sensitivity in an APsystem, including ELISA, Westernblotting and immunohistology.BluePhos ® AP Microwell Substrate50-88-02 BluePhos Phosphatase Substrate for ELISA 50 mL50-88-00 BluePhos Phosphatase Substrate for ELISA 600 mL50-88-01 BluePhos Phosphatase Substrate for ELISA 2700 mLpNPP Phosphatase Substrates50-80-00 pNPP Phosphatase Substrate Kit, 100 tabs & DEA Buffer 500 mL50-80-01 pNPP Substrate, 5 mg tablets 100 tabsStop Solutions50-89-00 APstop Solution (10X) (formerly BluePhos Stop Solution) 200 mLGaithersburg, MD 20878Phone: 800.638.3167Fax: 301.948.0169www.kpl.comISO 9001:2000 RegisteredTo order or for more information, contact us at 800.638.3167 / 301.948.7755,fax 301.948.0169 or visit us at www.kpl.com.ML347-02For research use only.BluePhos is a trademark of KPL and is covered by U.S. Patent No. 5,916,746.© 2007 KPL, Inc. All rights reserved.SEE MORE with KPL!