KPL ELISA Brochure

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AP SubstratesChemiluminescent ELISA: A constantamount of antigen (mouse IgG)was coated onto a microtiter plate.The plate was probed with varyingconcentrations of goat anti-mouseIgG (H+L) AP conjugate, includingKPL’s standard AP conjugate andReserveAP conjugate, followed byKPL’s PhosphaGLO ChemiluminescentAP Substrate.KPL offers a range of sensitive substratesfor the detection and quantificationof phosphatase (AP) activity.They provide a choice of intense colorsfor ELISA and blotting applications.ELISA• FirePhos TM AP MicrowellSubstrateConclusion: The results indicate that ReserveAP conjugate offers superior performance to our standardAP conjugate with a ~3-fold lower amount of conjugate required for detection.ReserveAP TM Conjugates Ordering InformationCatalog# Description Size0751-1001 Goat Anti-Human IgA (α) 1.0 mg0751-1002 Goat Anti-Human IgG (γ) 1.0 mg0751-1003 Goat Anti-Human IgM (µ) 1.0 mg0751-1004 Goat Anti-Human IgE (ε) 1.0 mg0751-1006 Goat Anti-Human IgG (H+L) 1.0 mg0751-1007 Goat Anti-Human IgA+IgG+IgM (H+L) 1.0 mg2151-1002 F(ab’) 2 Anti-Human IgG (γ) 0.5 mg4751-1002 Goat Anti-Human IgG (γ) liquid 1.0 mg4751-1003 Goat Anti-Human IgM (µ) liquid 1.0 mg4751-1006 Goat Anti-Human IgG (H+L) liquid 1.0 mg0751-1802 Goat Anti-Mouse IgG (γ) HSA 1.0 mg0751-1803 Goat Anti-Mouse IgM (µ) HSA 1.0 mg0751-1806 Goat Anti-Mouse IgG (H+L) HSA 1.0 mg0751-1809 Goat Anti-Mouse IgG +IgM (H+L) HSA 1.0 mg4751-1802 Goat Anti-Mouse IgG (γ) HSA, liquid 1.0 mg4751-1806 Goat Anti-Mouse IgG (H+L) HSA, liquid 1.0 mg151-18-01 Goat Anti-Mouse IgA (α) HSA 0.5 mg0751-1807 Goat Anti-Mouse IgA+IgG+IgM (H+L) HSA 1.0 mg0751-1506 Goat Anti-Rabbit IgG (H+L) 1.0 mg0751-1516 Goat Anti-Rabbit IgG (H+L) HSA 1.0 mg4751-1506 Goat Anti-Rabbit IgG (H+L), liquid 1.0 mg4751-1516 Goat Anti-Rabbit IgG (H+L) HSA, liquid 1.0 mgHSA=Human Serum AdsorbedVisit our website at www.kpl.com for a complete listing of ReserveAP conjugates. Toorder or for more information on KPL’s protein research products, contact us at800.638.3167 / 301.948.7755, FAX 301.948.0169 or visit us at www.kpl.com.ML349-04For research use only.©2007 KPL, Inc. All rights reserved.• BluePhos ® AP MicrowellSubstrate• pNPP Phosphatase SubstrateBlotting• FirePhos AP MembraneSubstrate• BCIP/NBT Phosphatase Substrate• PhosphaGLO AP Substrate• PhosphaGLO TM Reserve APSubstrateWhichever substrate you choose,enjoy the benefits of excellent signalto-noisewith higher sensitivity andlower background than that of otherAP substrates. Visit www.kpl.comfor more information.Gaithersburg, MD 20878Phone: 800.638.3167Fax: 301.948.0169www.kpl.comISO 9001:2000 RegisteredSEE MORE with KPL!
BacTrace ® Anti-Bacteria AntibodiesBacTrace ® E.coli O157:H7 Antibody labeled with DyLight 488 (green) directly detects E.coli O157:H7. BacTrace Salmonella (CSA-1) Antibody labeled with DyLight549 (red) directly detects Salmonella. (DyLight labeling performed on a custom basis.)BacTrace Antibodies to Food Pathogens and Other Infectious AgentsKPL produces a unique line of BacTrace ® antibacteriaantibodies for the detection of foodpathogens and other infectious agents affectinghumans and animals. They are purified tooffer excellent specificity and sensitivity inhigh performance immunoassays such asELISA, Western blotting, lateral flow andmicroscopic analysis. Many leading diagnostickit manufacturers use BacTrace antibodies intheir diagnostic kits. See our growing line ofBacTrace antibodies:• Anti-Salmonella• Anti-Salmonella, Molecular Grade• Anti-E.coli O157:H7• Anti-E.coli O157:H7, Molecular Grade• Anti-Listeria, genus-specific• Anti-Campylobacter, genus specific• Anti-Helicobacter pylori• Anti-Borrelia species• Anti-Borrelia burgdorferi• Anti-Renibacterium salmoninarumBacTrace antibodies offer a range of benefits:• Sensitivity: nanogram detection of bacterialantigens within hours.• Specificity: recognize both cell surface andintracellular antigens of bacterial cells.• Versatility: ideal for screening assays in theresearch lab, environmental monitoring andfor food and beverage analysis in the industrialsetting.• Positive controls available for eachBacTrace antibody.Anti-Salmonella and E.coli O157:H7,Molecular GradeNew molecular grade antibodies to Salmonellaand E. coli O157:H7 are recent additions to ourBacTrace line. These antibodies are speciallyprocessed and QC-tested to ensure that contaminatingSalmonella or E. coli DNA is notdetectable by real-time PCR or other molecularmethods.SEE MORE with KPL!
Page 1 and 2: Enzyme-Linked ImmunoSorbent AssaySe
Page 3 and 4: Praying MantisSee more with KPL ELI
Page 5 and 6: Comparison of SureBlue and SureBlue
Page 7 and 8: HRP ELISA KitsProtein Detector TM H
Page 9 and 10: AP ELISA KitsProtein Detector TM Al
Page 11 and 12: Immunoassay BuffersSave preparation
Page 13 and 14: HRP ELISA Color SubstratesReliable
Page 15 and 16: AP ELISA Color SubstratesSee your E
Page 17: ReserveAP TMConjugatesSpice up your
Page 21 and 22: Peroxidase ConjugatesKPL Peroxidase
Page 23: Ordering InformationCatalog # Descr

KPL ELISA Brochure

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