Basics of Biosafety in Biotech Labs

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UltraViolet LightNot Recommended for Disinfection• Causes: erythema, harms skin and eyes• Poor penetration: Requires direct exposure• Germicidal wavelengths (≤ 280 nm): causechemical reactions (damages rubber, glue)• Germicidal effect reduced above 70% R.H.• Bulb age affect wavelength output• Temperature also affect wavelength output• Dust and dirt on bulb reduce efficacy• Distance from UV source affects efficacyTurn it off after 15 minutes!
Medical Sharps• Medical sharps designed to cut (needles, razorblades, scalpels).• Dispose them immediately• Do not recap needles or leave a sharpunattended.• Only dispose them in designated containers.• Medical sharp injury: greatest risk forBBPinfection during work.
Page 1 and 2:
Basics of Biosafety inBiotech LabsM
Page 3 and 4:
اتفبق یه لحظِ،‏ ػَ
Page 7 and 8:
Risk Assessment• Identify hazard
Page 9 and 10:
References - Risk AssessmentBiosafe
Page 11 and 12: More References• Biohazard Recogn
Page 13 and 14: Risk Group 1• RG1 (a larg number
Page 15 and 16: cont.• “Ecotropic” viruses in
Page 17 and 18: Risk Group 3RG3:- serious or lethal
Page 19 and 20: Pathogen Characteristics• Route o
Page 21 and 22: Infectious Dose (ID50)• M. tuberc
Page 23 and 24: Precautions• Practices plus Physi
Page 25 and 26: Biosafety Level 1Good Microbiologic
Page 27 and 28: How Does Biosafety Level 2(BSL-2) D
Page 29 and 30: Personal Protective Equipment• La
Page 31 and 32: Wash your hands!!!Hand Hygiene ():
Page 33 and 34: سناریو های فرضی• د
Page 35 and 36: سئَاالتی وِ‏ ثبیس
Page 37 and 38: سئَاالتی وِ‏ ثبیس
Page 39 and 40: پبسد• ID50 is only one factor
Page 41 and 42: پبسد• Risk of immunocompromis
Page 43 and 44: پبسد• Eye injury: serious con
Page 45 and 46: Fume Hood• Fume Hood - Protects y
Page 47 and 48: Biological Safety Cabinet (BSC)Bios
Page 49 and 50: A-used-to-be-a-BSC• Bunsen burner
Page 51 and 52: Centrifugation Precautions• Train
Page 53 and 54: Hazard Communication• Purpose: In
Page 55 and 56: Biological Exposure Response1. Perf
Page 57 and 58: Spill Protocol• Plan ahead• Be
Page 59 and 60: DisinfectionThe total destruction o
Page 61: Factors Influemcing Bleach Efficacy
Page 66 and 67: Mixed Hazards:Chemical & Biological
Page 68 and 69: Animal Biosafety• Intrinsic risks
Page 70 and 71: How Does ABSL-2 DifferFrom ABSL-1?
Page 72 and 73: When Is ABSL-2 Appropriate?• Immu
Page 74 and 75: Plant Biosafety Levels• BSL1-P•
Page 76 and 77: سٌبریَ‏ یهلَلِ‏ و
Page 78 and 79: سٌبریَ‏ 2• ‏ًظز ضو
Page 80 and 81: سٌبریَ‏ 3ثیىبری ٍآ
Page 82 and 83: Summary• Risk assessment & Planni
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Basics of Biosafety in Biotech Labs

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