Basics of Biosafety in Biotech Labs

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Mixed Hazards:Chemical & Biological• Door closed: negative air pressure• Limit/Restrict access to lab• Use hazard communication - signs/labels• Minimize aerosol production• Containment: fume hood for chemicals• PPE: gloves, lab coats, safety glasses, respirators insome cases• High degree of caution with sharps, or avoid• Decontaminate surfaces & equipment• Exposure response plan and Spill protocol tailored tohazard
Biological Toxins• Produced by some biological agents• Toxin itself not infectious nor propagating• Affect critical physiological processes• Very low doses cause symptoms• LD50 – dose lethal to 50% of exposed population• Some are environmentally stable• Antidote/ Vaccine may be available• Treat as chemical hazard if biological agent not present• Maintain list of toxins work with in the lab• Post a warning sign when toxin is in use
Page 1 and 2:
Basics of Biosafety inBiotech LabsM
Page 3 and 4:
اتفبق یه لحظِ،‏ ػَ
Page 7 and 8:
Risk Assessment• Identify hazard
Page 9 and 10:
References - Risk AssessmentBiosafe
Page 11 and 12:
More References• Biohazard Recogn
Page 13 and 14:
Risk Group 1• RG1 (a larg number
Page 15 and 16: cont.• “Ecotropic” viruses in
Page 17 and 18: Risk Group 3RG3:- serious or lethal
Page 19 and 20: Pathogen Characteristics• Route o
Page 21 and 22: Infectious Dose (ID50)• M. tuberc
Page 23 and 24: Precautions• Practices plus Physi
Page 25 and 26: Biosafety Level 1Good Microbiologic
Page 27 and 28: How Does Biosafety Level 2(BSL-2) D
Page 29 and 30: Personal Protective Equipment• La
Page 31 and 32: Wash your hands!!!Hand Hygiene ():
Page 33 and 34: سناریو های فرضی• د
Page 35 and 36: سئَاالتی وِ‏ ثبیس
Page 37 and 38: سئَاالتی وِ‏ ثبیس
Page 39 and 40: پبسد• ID50 is only one factor
Page 41 and 42: پبسد• Risk of immunocompromis
Page 43 and 44: پبسد• Eye injury: serious con
Page 45 and 46: Fume Hood• Fume Hood - Protects y
Page 47 and 48: Biological Safety Cabinet (BSC)Bios
Page 49 and 50: A-used-to-be-a-BSC• Bunsen burner
Page 51 and 52: Centrifugation Precautions• Train
Page 53 and 54: Hazard Communication• Purpose: In
Page 55 and 56: Biological Exposure Response1. Perf
Page 57 and 58: Spill Protocol• Plan ahead• Be
Page 59 and 60: DisinfectionThe total destruction o
Page 61 and 62: Factors Influemcing Bleach Efficacy
Page 63: Medical Sharps• Medical sharps de
Page 68 and 69: Animal Biosafety• Intrinsic risks
Page 70 and 71: How Does ABSL-2 DifferFrom ABSL-1?
Page 72 and 73: When Is ABSL-2 Appropriate?• Immu
Page 74 and 75: Plant Biosafety Levels• BSL1-P•
Page 76 and 77: سٌبریَ‏ یهلَلِ‏ و
Page 78 and 79: سٌبریَ‏ 2• ‏ًظز ضو
Page 80 and 81: سٌبریَ‏ 3ثیىبری ٍآ
Page 82 and 83: Summary• Risk assessment & Planni
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Basics of Biosafety in Biotech Labs

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