Analtech Catalog.pdf

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RPS Reversed Phase PlatesThe basis of retention in Reversed Phase Chromatography is just theopposite of that seen in normal phase (e.g., silica gel) chromatography.In normal phase chromatography the adsorbent is a polar material.Adsorption occurs as a result of polar interactions. Thus, in normal phasechromatography, more polar sample components will be more tightlyadsorbed and will exhibit lower Rf values than less polar sample components.Rf values can be increased by making the mobile phase more polar.In Reversed Phase Chromatography the adsorbent is a relatively nonpolarmaterial. Adsorption occurs as a result of nonpolar interactions. Thus, inReversed Phase Chromatography less polar sample components will bemore tightly adsorbed and will exhibit lower Rf values than more polar samplecomponents. In Reversed Phase Chromatography Rf values can generally beincreased by making the mobile phase less polar.Analtech Reversed Phase Separation (RPS) UNIPLATES are a hydrocarbonimpregnated silica gel. The hydrocarbon is adsorbed onto the silica gelsurface and is not chemically anchored to the particle. The impregnated RPSUNIPLATE offers several advantages over the bonded RP18 type TLC platesalong with some limitations.The main advantages of the hydrocarbon impregnated Reversed Phase plateare compatibility with aqueous solutions and parallel behavior to most C18HPLC packings. The RPS UNIPLATE will accept 100% water either as themobile phase or spotting solvent. This is a great advantage when working withpolar sample components such as proteins and peptides.Reversed Phase UNIPLATES(Hydrocarbon Impregnated ≈ 5% carbon load)25 Plates/Box, 15μm particle, 60Å pore, 250μm Thickness unless otherwise notedPlate Size (cm) Cat. no. RPS cat. no. RPSF20 x 20 50011 5201120 x 20 (scored) 50511 5251110 x 20 50021 5202110 x 20 (scored) 50521 525215 x 20 50031 52031Preparative (500µm)20 x 20 50012 5201220 x 20 (scored) 50512 5251210 x 20 50022 520225 x 20 50032 52032Preparative (1000µm)20 x 20 50013 52013Hydrocarbon Impregnated• Less expensive than bonded phaseTLC plates• Aqueous compatibility for polar samples• Inorganic binder• Rapid, inexpensive methodsdevelopment for HPLC• Expanded flexibility to a wide range ofapplicationsThe RPS impregnated UNIPLATEapproximates the adsorptioncharacteristics of the bonded C18 modifiedsilica gels. Therefore, RPS Reversed PhaseUNIPLATES are an excellent mediumfor rapid, inexpensive screening ofchromatographic conditions in HPLCmethod development. Mixtures of water orbuffers plus a polar organic modifier (eg.,Methanol, acetonitrile, THF, etc.) are easilyscouted with RPS UNIPLATES. However,since the hydrocarbon is adsorbed butnot chemically anchored to the silicagel, the RPS UNIPLATES cannot toleratenonpolar organic solvents. Ethyl Acetateor less polar organic solvents will strip thehydrocarbon from the silica gel support.(For organic solvent compatible plates seethe UNIBOND series of HPTLC plates onpage 20.)13
Modifiied Silica PlatesAnaltech manufactures a number of modified silica gelson a routine basis. Often the addition of some specificmaterial to the base adsorbent aids in discrimination ofparticular analytes. The most commonly used modifiedplates are listed here. Modification can be accomplishedin two ways. The additive material can be dissolved inthe solution used to prepare the spreading slurry, or afinished TLC plate can be impregnated with the desiredagent. If you have some particular requirement other thanthose shown, call Analtech for prices and delivery.Sodium Hydroxide (0.1N) ModifiedSilica Gel G & GF UNIPLATESInstead of water, 0.1 N sodium hydroxide is used toprepare the slurry for these plates. This makes the platesmore basic and contributes to improved separation ofcertain compounds, particularly organometallics andsome acidic classes.25 Plates/Box, 250μm Thickness, 10μm particle, 60Å porePlate Size(cm) Catalog g number gf20 x 20 68011 6901110 x 20 68021 690215 x 20 68031 69031Carbomer (0.33%) ModifiedSilica Gel H UNIPLATESThese very specialized plates are prepared according toseveral Pharmacopoeia methods. They are used in theanalysis of mannitol/sorbitol.NOTE: These plates are very fragile and will mostdefinitely arrive with silica flaked off of the plate. Thishas always been the case with these very custom TLCplates. This warning is given because these plates are notreturnable.25 Plates/Box, 750μm Thickness, 20x20 cm, 19μm particle, 60Å poreCatalog #70019Silver Nitrate Impregnated (%) Silica Gel UNIPLATES (for Argentation Chromatography)These UNIPLATES are impregnated with between 5% and 20% silver nitrate which modifies the separation characteristics of theadsorbent layer to permit increased discrimination of certain compounds, particularly those containing carbon-carbon double bonds.They are available with or without a fluorescent indicator.NOTE: The concentration of silver nitrate (5, 10, 15 or 20%) must be specified at the time of order.25 Plates/Box, 10μm, 60Å pore, 250μm Thickness unless notedPlate Size(cm)CatalogNo. w/o fCatalogno. w/ f20 x 20 28011 2901110 x 20 28021 290215 x 20 28031 29031Preparative (500µm)20 x 20 28012 29012Preparative (1000µm)20 x 20 28013 2901314
Page 4: Customer SupportAnaltech maintains
Page 8: Layer ThicknessAnaltech UNIPLATES a
Page 17 and 18: Hard Layer Plates• High performan
Page 19 and 20: Non-Silica Plates25 Plates/Box, 250
Page 21 and 22: High Performance PlatesNormal Phase
Page 23 and 24: Flexible-backed TLC Sheets• Light
Page 25 and 26: Solid Phase ExtractionSPE Cartridge
Page 27 and 28: Other Bulk SilicasSilica Gel packin
Page 29 and 30: TLC Educational ResourcesApplied Th
Page 32 and 33: Graduated Disposable Micropipettes
Page 34 and 35: tlc autospotterCheck out a videodem
Page 36 and 37: Rotors and Accessories for the Cycl
Page 38 and 39: Analtech’s glass TLC developing c
Page 40 and 41: Plate Conditioning ApparatusCatalog
Page 42 and 43: Visualization EquipmentTLC Reagent
Page 44 and 45: Lightweight UV CabinetThis compact,
Page 46 and 47: UV TransilluminatorsThese transillu
Page 48 and 49: ChromaDoc-It TLC Imaging SystemThis
Page 50 and 51: Introduction to SMT SAM-C18Columns
Page 52 and 53: Unique FeatureHighly versatile; str

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