Analtech Catalog.pdf

Customer SupportAnaltech maintains a complete customer support and applicationsdevelopment laboratory at its headquarters in Newark, Delaware. Ourtechnical staff is prepared to assist Analtech customers with questionsconcerning TLC methodology and may provide effective solutions to specificapplications. If you have a question, contact Analtech’s Technical Service at800-441-7540 or by email at techinfo@analtech.com.Analtech WorldwideAnaltech offers the widest selection of adsorbent layers, plate sizes, andprecoated TLC plate styles available anywhere. We also offer a complete lineof TLC apparatus and accessories.Analtech UNIPLATES are routinely used in thousands of Analytical, Research,Clinical, Industrial, and Quality Control Laboratories throughout the world.As well as selling direct to customers, Analtech products are represented byover 45 distributors worldwide, including Alltech, Cole-Parmer, Fisher, Sigma-Aldrich, and VWR.At Analtech we are committed to advancing TLC as a practical, more costeffective analytical method. All of our products are the highest quality availableand our customer service is prompt and friendly.Analtech OnlineEvery product we sell (many more than can fit into the catalog) is nowavailable for viewing and purchasing from our quick searchable databaseonline at www.analtech.com.Want to see a CycloGraph or an AutoSpotter in operation? Check out thedemonstration videos on the web site.We also have an exciting monthly e-newsletter, the Thin Layer Chronicles,featuring the latest news and developments in Thin Layer Chromatography.Just sign up at www.analtech.com. Don’t forget to check out our data sheetsfor select products and customer surveys to keep in touch with customerneeds.About AnaltechSome of our web sections are listed below:Online CatalogOur entire line of products is listed here in anewly formatted searchable database. Thissection is continually updated.Technical CenterResources here include Application Notes andProduct Data Sheets. We also keep you up todate on recently published TLC abstracts whilemaking sure to mix in some classics.SupportThis section contains TLC Fact Sheets andTechnology Reports. There is helpfulinformation here on a variety of TLC topics,including when plate activation is necessaryand how a plate can be pre-developed toproduce nice thin sample bands instead ofcircular spots.training videosWhether you want to see a CycloGraph inaction or a TLC AutoSpotter’s accuracy, or evensee how to snap one of our scored plates, go towww.analtech.com for the latest videos.PHONE: 800.441.7540 • 302.737.6960 FAX: 302.737.7115WWW.ANALTECH.COM3

method, however, gravity does not slow the speed of theseparation as much.Another specialized method of separation is2-dimensional development. With this a single spot isapplied near a corner of the plates. After chromatographyin the first direction, the plate is dried, rotated 90° anddeveloped in the second dimension.with another mobilephase.Evaluation of the ChromatogramEvaluation can be as simple as a visual inspectionunder ultra violet light to determine the existence of acomponent. Or it could be as complicated as exposingthe plate to a chemical spray, followed by heat charringand densitometric scanning to compare the sampleto standards on the plate and achieve an accuratemeasurement of how much of a component is on theplate.Anyway you look at evaluation, the one constant is thecalculation of the retention factor (Rf value). This valueis measured as the relative distance traveled from thespot origin to the mobile phase solvent front. As longas all other variables are kept constant, the Rf value ofa sample component should remain constant and bereproducible.Inherent Advantages of TLCTLC permits the simultaneous analysis of many samplesin the same time period required for one HPLC analysis.Samples and standards are analyzed under exactly thesame conditions rather than serially as in HPLC.TLC uses a fresh, new adsorbent for each analysis. Thisinsures reproducible results and eliminates the HPLCproblems of adsorbent contamination from previousanalyses and lost efficiency from worn columns. Also,with the use of smaller developing tanks, the amount ofdeveloping solvent required is much less than with HPLC.TLC simplifies methods development. Unlike chromatographyperformed on columns, the sample components remain in theadsorbent and can always be located and retrieved for furtherexperimentation.Applications of TLCTLC is generally used for one of three purposes.1) QUALITATIVE AnalysisTo determine the presence or absence of a particularsubstance in a mixture. A rough estimation of the level of thesubstance may also be performed.2) QUANTITATIVE AnalysisTo determine, precisely and accurately, the amount of aparticular substance in a sample mix.3) PREPARATIVE AnalysisTo purify and isolate a particular substance by separating itfrom any contaminants.All three cases share the common procedures of sampleapplication, chromatographic separation, and samplecomponent visualization. The conditions under which theseprocedures are performed vary according to the required endresult. Some typical requirements and conditions are shownbelow.Factor Qualitative Quantitative PreparativeSample Volume 2-50µL 0.1-0.5µL 50-1000µLSample Amount 10-200µg 50-500ng 5-500mgSample Appl. Precision ±10% ±1% NALayer Thickness 250µm 150µm 500-2000µmDevelopment Distance 12 cm 5 cm 15 cmDevelopment Time 15-30 min 4-8 min 25-60 minVisualization Type Visual Visual &DensitometricVisualTLC does not require complex, costly maintainedinstrumentation. The investment for performing successfulTLC can be ten to one hundred times less than for HPLC.6

Layer ThicknessAnaltech UNIPLATES are 250 microns thick for regular analytical TLC and between 500 and 2000microns for preparative TLC. Tapered preparative UNIPLATES have a wedge-shaped layer 300µm to 1700µm with a700µm preadsorbent area (see notes on page 9). High Performance UNIPLATES have a thinner layer of adsorbentbetween 150-200 microns. All thickness designations are nominal. Actual thicknesses may vary but are consistent frombatch to batch.Plate SizeUNIPLATES are available in the following standard sizes: 20 x 20 cm,10 x 20 cm, 5 x 20 cm as well as 2.5 x 10 cm and 2.5x 7.5 cm. The standard size for High Performance UNIPLATES (HPTLC) is 10 x 10 cm, although they are available in othersizes. Analtech will be happy to supply your special TLC plate requirements (such as 20 x 40 cm plates) whenever it iswithin our capability to do so. Please contact Technical Services for information regarding feasibility and pricing.Call 800-441-7540 or 302-737-6960 (or email techinfo@analtech.com).Preadsorbent UNIPLATES• 3cm wide sample application zone• Concentrates sample spots on the plate• Simplifies sample preparation and application• Channeled version availableTLC Plate FormatsPreadsorbent UNIPLATES have an inert spotting zonealong the bottom edge of the plate. This sample applicationregion serves as a “holding zone” for sample spots untildevelopment of the plate is initiated. There is no significantresolution of sample components by the stationary phase inthis region. All soluble components migrate with the solventfront, concentrating diffuse sample spots into vertically narrowbands.Preadsorbent plate (in stages)The adsorbent zone extends from the upper edge of the preadsorbent zone and covers the remaining area of theTLC plate. This zone exhibits the selective retention of sample components characteristic of the adsorbent employed.Separation of sample components occurs in the adsorbent zone in the same way as on a standard TLC plate.Preconcentration of the sample results in compact zones at the beginning of the separation. Also, resolution and sensitivityare less dependent on sample application technique since all samples are concentrated to narrow bands regardless of thesize or position in the zone of the sample spots.Preadsorbent UNIPLATES are available in a variety of adsorbents and sizes throughout the catalog.PHONE: 800.441.7540 • 302.737.6960 FAX: 302.737.7115WWW.ANALTECH.COM7

RPS Reversed Phase PlatesThe basis of retention in Reversed Phase Chromatography is just theopposite of that seen in normal phase (e.g., silica gel) chromatography.In normal phase chromatography the adsorbent is a polar material.Adsorption occurs as a result of polar interactions. Thus, in normal phasechromatography, more polar sample components will be more tightlyadsorbed and will exhibit lower Rf values than less polar sample components.Rf values can be increased by making the mobile phase more polar.In Reversed Phase Chromatography the adsorbent is a relatively nonpolarmaterial. Adsorption occurs as a result of nonpolar interactions. Thus, inReversed Phase Chromatography less polar sample components will bemore tightly adsorbed and will exhibit lower Rf values than more polar samplecomponents. In Reversed Phase Chromatography Rf values can generally beincreased by making the mobile phase less polar.Analtech Reversed Phase Separation (RPS) UNIPLATES are a hydrocarbonimpregnated silica gel. The hydrocarbon is adsorbed onto the silica gelsurface and is not chemically anchored to the particle. The impregnated RPSUNIPLATE offers several advantages over the bonded RP18 type TLC platesalong with some limitations.The main advantages of the hydrocarbon impregnated Reversed Phase plateare compatibility with aqueous solutions and parallel behavior to most C18HPLC packings. The RPS UNIPLATE will accept 100% water either as themobile phase or spotting solvent. This is a great advantage when working withpolar sample components such as proteins and peptides.Reversed Phase UNIPLATES(Hydrocarbon Impregnated ≈ 5% carbon load)25 Plates/Box, 15μm particle, 60Å pore, 250μm Thickness unless otherwise notedPlate Size (cm) Cat. no. RPS cat. no. RPSF20 x 20 50011 5201120 x 20 (scored) 50511 5251110 x 20 50021 5202110 x 20 (scored) 50521 525215 x 20 50031 52031Preparative (500µm)20 x 20 50012 5201220 x 20 (scored) 50512 5251210 x 20 50022 520225 x 20 50032 52032Preparative (1000µm)20 x 20 50013 52013Hydrocarbon Impregnated• Less expensive than bonded phaseTLC plates• Aqueous compatibility for polar samples• Inorganic binder• Rapid, inexpensive methodsdevelopment for HPLC• Expanded flexibility to a wide range ofapplicationsThe RPS impregnated UNIPLATEapproximates the adsorptioncharacteristics of the bonded C18 modifiedsilica gels. Therefore, RPS Reversed PhaseUNIPLATES are an excellent mediumfor rapid, inexpensive screening ofchromatographic conditions in HPLCmethod development. Mixtures of water orbuffers plus a polar organic modifier (eg.,Methanol, acetonitrile, THF, etc.) are easilyscouted with RPS UNIPLATES. However,since the hydrocarbon is adsorbed butnot chemically anchored to the silicagel, the RPS UNIPLATES cannot toleratenonpolar organic solvents. Ethyl Acetateor less polar organic solvents will strip thehydrocarbon from the silica gel support.(For organic solvent compatible plates seethe UNIBOND series of HPTLC plates onpage 20.)13

Modifiied Silica PlatesAnaltech manufactures a number of modified silica gelson a routine basis. Often the addition of some specificmaterial to the base adsorbent aids in discrimination ofparticular analytes. The most commonly used modifiedplates are listed here. Modification can be accomplishedin two ways. The additive material can be dissolved inthe solution used to prepare the spreading slurry, or afinished TLC plate can be impregnated with the desiredagent. If you have some particular requirement other thanthose shown, call Analtech for prices and delivery.Sodium Hydroxide (0.1N) ModifiedSilica Gel G & GF UNIPLATESInstead of water, 0.1 N sodium hydroxide is used toprepare the slurry for these plates. This makes the platesmore basic and contributes to improved separation ofcertain compounds, particularly organometallics andsome acidic classes.25 Plates/Box, 250μm Thickness, 10μm particle, 60Å porePlate Size(cm) Catalog g number gf20 x 20 68011 6901110 x 20 68021 690215 x 20 68031 69031Carbomer (0.33%) ModifiedSilica Gel H UNIPLATESThese very specialized plates are prepared according toseveral Pharmacopoeia methods. They are used in theanalysis of mannitol/sorbitol.NOTE: These plates are very fragile and will mostdefinitely arrive with silica flaked off of the plate. Thishas always been the case with these very custom TLCplates. This warning is given because these plates are notreturnable.25 Plates/Box, 750μm Thickness, 20x20 cm, 19μm particle, 60Å poreCatalog #70019Silver Nitrate Impregnated (%) Silica Gel UNIPLATES (for Argentation Chromatography)These UNIPLATES are impregnated with between 5% and 20% silver nitrate which modifies the separation characteristics of theadsorbent layer to permit increased discrimination of certain compounds, particularly those containing carbon-carbon double bonds.They are available with or without a fluorescent indicator.NOTE: The concentration of silver nitrate (5, 10, 15 or 20%) must be specified at the time of order.25 Plates/Box, 10μm, 60Å pore, 250μm Thickness unless notedPlate Size(cm)CatalogNo. w/o fCatalogno. w/ f20 x 20 28011 2901110 x 20 28021 290215 x 20 28031 29031Preparative (500µm)20 x 20 28012 29012Preparative (1000µm)20 x 20 28013 2901314

Magnesium Acetate (%) ModifiedSilica Gel H & HF UNIPLATESThese Silica Gel H UNIPLATES are prepared with 5%,7.5%, or 10% magnesium acetate as the slurry liquid.The addition of magnesium acetate decreases theacidity of the layer and allows increased discrimination ofphospholipids. The concentration of magnesium acetateadded to the layer must be specified at the time of order(7.5% is most commonly used)25 Plates/Box, 19μm, 60Å pore, 250μm Thickness unless notedPlate Size(cm)Catalogno. hCatalogno. hf20 x 20 88011 8901110 x 20 88021 890215 x 20 88031 89031Preparative (500µm)20 x 20 88012 89012Potassium Oxalate (1%) ModifiedSilica Gel H & HF UNIPLATESPrepared with 1% potassium oxalate during slurrypreparation, these plates acquire a separationcharacteristic which aids in the discrimination ofpolyphosphoinositides.Ammonium Sulfate (5%) ModifiedSilica Gel H & HF UNIPLATES (Self-Charring)A common method for visualizing organic compoundson a silica gel TLC plate is charring. This is usuallyaccomplished by spraying the plate with dilute sulfuric acidfollowed by heating. Organic substances appear as brownto black spots against a white background. This methodis comparable in sensitivity to the visualization of UVabsorbingcompounds against a fluorescent background.Silica Gel H UNIPLATES containing ammonium sulfateeliminate the need to use messy sulfuric acid for charring.Just evaporate the developing solvent until the plateis completely dry, then heat at 150°-200°C for 30 to 60minutes to achieve charring. The Analtech VPF chamberis specifically designed for this application. Otherpercentages of ammonium sulfate may be specified.25 Plates/Box, 250μm Thickness, 19μm particle, 60Å porePlate Size(cm)CatalogNo. hcatalogno. hf20 x 20 74011 7501110 x 20 74021 750215 x 20 74031 7503125 Plates/Box, 250μm Thickness 19μm particle, 60Å porePlate Size(cm)CatalogNo. hCatalogno. hf20 x 20 86011 8701110 x 20 86021 870215 x 20 86031 87031PHONE: 800.441.7540 • 302.737.6960 FAX: 302.737.7115WWW.ANALTECH.COM15

Hard Layer Plates• High performance silica gel providesfaster separations, higher resolution,and improved sensitivity.• Enhanced abrasion resistance offerseasier handling.• Harder surface simplifies sampleapplication.• Hard layer surface is easily written on.• Compatible with organic solvents• Faster development comparedto silica gel 60• Fluorescent plates are ideal forUV shadowingResearch is what I’m doing when Idon’t know what I’m doing.Wernher Von BraunHard Layer Silica Gel HL & HLF UNIPLATES (Organic Binder)These are the most rugged silica gel plates available. They not only easehandling and sample application, but also permit the use of up to 80%water in the developing solvent without loss of adherence of the adsorbentlayer to the glass plate. Organic binder plates are recommended for allTLC applications except those that use vigorous charring for visualization.Virtually all other visualization methods are free from interference from theorganic binder.Hard Layer Silica Gel UNIPLATES represent an advancement in thinlayer adsorbent technology. Special high performance silica gel withsmall average diameter and narrow particle size distribution resultsin separations 25% to 30% faster than with most other TLC plates.Separated spots are more compact and better resolved, which allowsvisualization at lower sample levels. The enhanced abrasion resistancemeans easier handling and sample application because plate surfacegouging is virtually eliminated. Analtech Hard Layer UNIPLATES areavailable with either organic or inorganic binders. Binder choice has littleeffect on the adsorptive properties of the adsorbent layer.Hard Layer Silica Gel HL & HLF UNIPLATES25 Plates/Box, 250 μm Thickness, 10μm particle, 60Å porePlateSize (cm)scoredchanneledpreadsorbentzonecat. no.hlcat. no.hlf20 x 20 – – – 46011 4701120 x 20 Yes – – 46511 4751120 x 20 – Yes – 46911 4791120 x 20 – – Yes 43011 4401120 x 20 Yes Yes – 46711 4771120 x 20 Yes – – 46311* 47311*20 x 20 Yes – Yes 43511 4451120 x 20 Yes – Yes 43511* 44311*20 x 20 – Yes Yes 43911 4491120 x 20 Yes Yes Yes 43711 4471110 x 20 – – – 46021 4702110 x 20 Yes – – 46521 4752110 x 20 – Yes – 46921 4792110 x 20 – – Yes 43021 4402110 x 20 – Yes Yes 43921 449215 x 20 – – – 46031 470315 x 20 – Yes – 46931 479315 x 20 – – Yes 43031 44031* Specially scored to snap to eight 2.5 x 20 cm plates16PHONE: 800.441.7540 • 302.737.6960 FAX: 302.737.7115WWW.ANALTECH.COM

Hard Layer Silica Gel GHL & GHLF UNIPLATES(Inorganic Binder)These plates are 100% H20 resistant and are compatible with aqueousdeveloping solvents. They are less rugged than the plates with organicbinder but still provide improved handling and sample applicationcompared to Silica Gel G UNIPLATES and are tough enough to write onwith a soft pencil, if required.Hard Layer UNIPLATES with inorganic binder are recommended for allapplications including those where visualization occurs through strongcharring procedures or in any case in which reagent interaction with anorganic binder occurs.Hard Layer Silica Gel GHL & GHLF UNIPLATES25 Plates/Box, 250 μm Thickness, 15μm particle, 60Å porePlateSize (cm)scoredchanneledpreadsorbentzonecat. no.Ghlcat. no.Ghlf20 x 20 – – – 11011 2101120 x 20 Yes – – 11511 2151120 x 20 – Yes – 11911 2191120 x 20 – – Yes 41011 4201120 x 20 Yes Yes – 11711 2171120 x 20 Yes – – 11311* 21311*20 x 20 Yes – Yes 41511 4251120 x 20 Yes – Yes 41311* 42311*20 x 20 – Yes Yes 41911 4291120 x 20 Yes Yes Yes 41711 4271110 x 20 – – – 11021 2102110 x 20 Yes – – 11521 2152110 x 20 – Yes – 11921 2192110 x 20 – – Yes 41021 4202110 x 20 – Yes Yes 41921 429215 x 20 – – – 11031 210315 x 20 – Yes – 11931 219315 x 20 – – Yes 41031 42031• Layers tolerate 100% water.• Contains no organic materials.• Suitable for aggressive visualizationprocedures.• Standard scoring for 20 x 20 cmplates yields four 5 x 20 cm plates.• Channeled 20 x 20 cm plates have19 channels (see channeled adapterfor TLC Autospotter (page 32)• One box of scored 10 x 20 cmplates yields two hundred2.5 x 10 cm plates.* Specially scored to snap to eight 2.5 x 20 cm plates17

Non-Silica Plates25 Plates/Box, 250μm Thickness unless notedNearly spherical particle shape, 50 µm mean diameterPlate Size(cm)Cat. No.avicelcat. no.avicel f20 x 20 05011 0601110 x 20 05021 060215 x 20 05031 06031scored20 x 20 05511 0651110 x 20 05521 06521preparative (500µm)20 x 20 05012 0601210 x 20 05022 06022preparative (1000µm)20 x 20 05013 0601310 x 20 05023 06023Avicel® UNIPLATES(Microcrystalline Cellulose)These plates are coated with AVICEL microcrystallinecellulose manufactured by the American Viscose divisionof FMC Corp. The adsorbent consists of regeneratedalpha cellulose particles of nearly spherical shape and50 micron mean diameter. Compounds separated on thiscellulose tend to form more compact spots than on fibrouscellulose layers.25 Plates/Box, 250μm Thickness unless notedPlate Size(cm)CatalogNo. GCatalogno. GF20 x 20 03011 0401110 x 20 03021 040215 x 20 03031 04031Preparative (1000µm)20 x 20 03511 0451110 x 20 03521 04521Preparative (500µm)20 x 20 03012 0401210 x 20 03022 04022Preparative (1000µm)20 x 20 03013 0401310 x 20 03023 04023Alumina G & GF UNIPLATESUsing a slurry containing calcium sulfate hemihydrateas a binder, these plates are analogous to Silica Gel GUNIPLATES, but with aluminum oxide as the adsorbent.The pH is neutral to slightly basic. Coatings are available inboth analytical and preparative thicknesses.18

DEAE Avicel Cellulose UNIPLATESDiethylaminoethyl (DEAE) Cellulose carries positive charges at neutral and acidic pH. Commonly employed forseparation of negatively charged molecules by ion exchange chromatography. Hydrophilic nature of the cellulosesubstrate makes it well suited to ion exchange separations of delicate biomolecules. Plates are available withmixed layers containing both DEAE Cellulose and unmodified cellulose. Mixed-layer plates have lower ion exchangecapacity and may be expected to provide higher chromatographic mobility for negatively charged molecules.NOTE: HR plates contain a highly refined (HR) cellulose.25 Plates/Box, 250μm ThicknessPlate Size(cm)deae celluloseCat. No.avicelcat. no.avicel f20 x 20 36011A 36011AF10 x 20 36021A 36021AF5 x 20 36031A 36031AF9:1 Avicel:deae cellulose20 x 20 37011A 37011AF10 x 20 37021A 37021AF5 x 20 37031A 37031AF7.5:1 Avicel:deae cellulose20 x 20 38011A 38011AF10 x 20 38021A 38021AF5 x 20 38031A 38031AF15:2 hr cellulose:deae cellulose20 x 20 39011 39011F10 x 20 39021 39021F5 x 20 39031 39031FPHONE: 800.441.7540 • 302.737.6960 FAX: 302.737.7115WWW.ANALTECH.COM19

High Performance PlatesNormal PhaseHigh Performance UNIPLATES provide high resolutionseparations in less than five minutes with migration distances aslittle as 5 to 7 cm. These plates are prepared with high efficiencysilica gel having an 8 micron average particle diameter andnarrow particle size distribution. The adsorbent layers are only150 microns thick, resulting in more rapid solvent migration. Theadsorbent capacity is more than adequate for small samples usedin high performance TLC techniques. Typically, sample volumesfor high performance TLC are less than one microliter. Samplespots are normally smaller than one millimeter in diameter.Samples are spotted 1.0 to 1.5 cm from the bottom edge of theplate and 0.3 to 0.5 cm apart. Since smaller samples are applied,one 10 x 10 cm plate can separate as many samples as can beseparated on a 20 x 20 cm plate using standard TLC techniques.The smooth homogeneous surface of HPTLC UNIPLATES giveshigh signal-to-noise ratios for densitometric scanning, thusincreasing sensitivity and precision.Silica Gel HPTLC UNIPLATES (Organic Binder)25 Plates/Box, 150 μm Thickness, 8μm particle, 60Å porePlate Size (cm)cat. no.hp-hlcat. no.hp-hlf10 x 10 58077 5907710 x 20 58027 5902720 x 20 58017 59017scored10 x 10 (to 5 x 5) 58377 5937710 x 20 58527 59527preadsorbent10 x 10 60077 6107710 x 20 (low-form)* 60027 6102720 x 10 (high-form)* 60127 6112720 x 20 60017 61017* The preadsorbent zone on HPTLC UNIPLATES is 1.5 cm high. Low-form plateshave the preadsorbent zone along the 20 cm edge. High-form plates have thepreadsorbent zone along the 10 cm edge.• High efficiency 8 micron (ave) adsorbent withSilica Gel HPTLC UNIPLATES (Inorganic Binder)25 Plates/Box, 150 μm Thickness, 8μm particle, 60Å porePlate Size (cm)cat. no.hp-Ghlcat. no.hp-Ghlf10 x 10 56077 5707710 x 20 56027 5702720 x 20 56017 57017scored10 x 10 (to 5 x 5) 56377 5737710 x 20 56527 57527Unibond Amino (NH2) & Cyano (CN) HPTLCUNIPLATES25 Plates/Box, 150 μm Thickness, 8μm particle, 60Å porePlate Size (cm)cat. no.hp-NH2Fcat. no.hp-CNF10 x 10 22077 2307710 x 20 22027 2302720 x 20 22017 23017scorednarrow particle and pore size distribution• 150 micron adsorbent layer thickness for fastseparations• Homogeneous layer allows linear or radialseparations• Smooth adsorbent surface for noise freedensitometry10 X 20 22527 2352720PHONE: 800.441.7540 • 302.737.6960 FAX: 302.737.7115WWW.ANALTECH.COM

Reversed PhaseThe availability of both normal phase and ReversedPhase HPTLC UNIPLATES completes HPTLC as asensible, efficient and cost effective alternative techniquefor quantitative analysis. These Reversed Phaseadsorbents share the features and benefits of silica gelHPTLC plates: high efficiency adsorbents, 150 µm layerthickness, and smooth surface for densitometry.In the High Performance format, Analtech offers severalvarieties of supports. A hydrocarbon impregnatedlayer, similar to the analytical RPS UNIPLATE, offersReversed Phase Unibond Series HPTLC UNIPLATES25 Plates/Box, 150μm Thickness (Organic binder)DESCRIPTION PLATE SIZE CAT. NO.HP-RP18 10 x 10 62077HP-RP18F 10 x 10 63077HP-RP8F 10 x 10 09077HP-RP2F 10 x 10 08077HP-RP18 10 x 20 62027HP-RP18F 10 x 20 63027HP-RP8F 10 x 20 09027HP-RP2F 10 x 20 08027HP-RP18 20 x 20 62017HP-RP18F 20 x 20 63017HP-RP8F 20 x 20 09017HP-RP 20 x 20 08017Reversed Phase Hydrocarbon ImpregnatedHPTLC UNIPLATES25 Plates/Box, 150μm Thickness 8μm particle, 60Å pore(≈5% carbon load, Inorganic binder)plate size (CM)cat. no.HP-RPSCAT. NO.HP-RPSF10 x 10 54077 5507710 x 20 54027 55027complete aqueous solvent compatibility (see page 11 forcomplete details). Three nonpolar bonded supports in theUNIBOND series are available: RP2, RP8, and RP18.These fully silanized silica gels share completecompatibility with all organic solvents. However, due tothe extreme hydrophobic nature of the RP18 adsorbent,aqueous wettability is limited to approximately 60%water in the mobile phase. Wettability is correspondinglyhigher with the slightly more polar shorter chain RP seriesplates.6.05.04.03.02.0C8C18C2C8C18C2C2C8C18C2C8C181.0C18C2C8C18 C8C8MeOH 100 90 80 70 60 50 40 30 20 10 -H2O - 10 20 30 40 50 60 70 80 90 100C2C8X-AXIS IS SOLVENT MIXTUREY-AXIS IS Rf VALUESelectivity can be varied for a given sample bychoosing a UNIBOND Reversed Phase HPTLCplate of proper nonpolarity. The graph aboveillustrates Rf values for Rhodamine B, a polardyestuff, in mixtures of methanol & water forRP2, RP8, and RP18.C2C2C221

Flexible-backed TLC Sheets• Lighter and safer to handle thanglass; also unbreakable• Easy to cut with scissors to customizeplate size or isolate components• Available with Polyester or Aluminumbacking• Attach to reports for permanentrecord• Minimum storage space required• Inexpensive shipping due to weightFlexible Backed TLC Sheets (Plastic & Aluminum Backings)Analtech’s flexible backed TLC plates offer the same exacting specifications as with the classic glass backed plates assuringbatch-to-batch uniformity. Quality is controlled at each step in the manufacturing process – from incoming raw materials tooutgoing packaging.Comparison of flexible backings to glassPhysical PropertY Glass Plastic AluminumThickness (approx) 1.6 mm 0.2 mm 0.15 mmWeight (packaging, storage requirements high low lowTorsional strength ideal low relatively highTemperature stability high max. 185°C highSusceptible to breakage yes no noCan be cut with scissors no yes yesChemical resistanceAgainst solvents high high highAgainst mineral acids and conc. ammonia high high lowBinder stability in water depends very limited22

Flexible-backed TLC Sheetsadsorbent layerplate size(cm)platebackingthickness(microns)# sheetscat. no.w/o fcat. no.w/ fSilica Gel 20 x 20 plastic 200 25 158017 159017Silica Gel 2.5 x 7.5 plastic 200 200 158061 159061Silica Gel 20 x 20 aluminum 200 25 156017 157017Silica Gel 2.5 x 7.5 aluminum 200 200 156061 157061Silica Gel HR * 20x 20 plastic 200 25 154017 155017Diol Modified Silica Gel 20 x 20 aluminum 150 25 - 125017Microcrystalline Cellulose 20 x 20 plastic 100 25 105016 106016Microcrystalline Cellulose PEI 20 x 20 plastic 100 25 205016 206016Cellulose (fibrous) 20 x 20 plastic 100 25 166016 167016Aluminum Oxide, pH9 20 x 20 plastic 200 25 100016 101016Aluminum Oxide, pH9 20 x 20 aluminum 200 25 102016 103016Polyamide 20 x 20 plastic 100 25 117058 118058Reversed Phase RP18 20 x 20 aluminum 150 25 – 350016* Silica Gel HR is made with a higher purity silica gel and a different binder system than the regular silica plates use.Other Coatings Available on Flexible BackingsNot all coatings or TLC sheet sizes are listed in the table above. Call Technical Support for alternative sheet sizes.Additional coatings are available as follows:On an aluminum support - high performance silica gel, dimethyl, cyano, and amino-modified silica gelOn a plastic support - DEAE cellulose, acetylated cellulose, cation and anion exchange resinsPHONE: 800.441.7540 • 302.737.6960 FAX: 302.737.7115WWW.ANALTECH.COM23

Solid Phase ExtractionSPE CartridgesAnaltech SPE cartridges are prepacked with adsorbent and ready for use. Molded of medical grade polypropylene,each cartridge contains 1 cc of packing. A porous frit and outlet filter combination contain the packing and will removesample particulate down to 10 microns. In normal use SPICE sample preparation cartridges are compatible withorganic solvents and aqueous solutions. Precision Luer fittings provide leakproof liquid connections. The inlet is afemale lock type and the outlet is a male slip. This unique design enables SPICE cartridges to be used either with asyringe or a vacuum manifold. Syringe use is ideally suited for field sampling or for intermittent laboratory requirements.Repetitive sample prep applications are conveniently performed via a vacuum manifold. Typical flow for either method isapproximately one minute per 10 cc of liquid at 14psi. This insures sufficient sample residence time in the cartridge foreffective adsorption/desorption.48 cartridges/boxpacking milligrams catalog no.Silica Gel 300 01-00UNIBOND C2 400 01-08UNIBOND C8 400 01-09UNIBOND C18 400 01-10UNIBOND Amino 400 01-22UNIBOND Cyano 400 01-23Alumina (neutral) 1000 01-33Alumina (basic) 1000 01-34Alumina (acidic) 1000 01-35Florisil 500 01-96SPICE Bulk PackingsSPICE Packings are available in bulkquantities of 100 grams, 500 grams, 5kg and 10 kg. For largerquantities call for a pricequote. Silica gel andbonded phases arebased upon a 150Å poresilica gel, 35-75 µmparticle.packingamountunibond c2cat. no.unibond c8cat. no.unibondc18 cat. no.unibondaminocat. no.unibondcyanocat. no.100 grams 08010 09010 78010 22010 23010500 grams 08050 09050 78050 22050 230505,000 grams 08060 09060 78060 22060 2306010,000 grams 08070 09070 78070 22070 23070packingamountsilica gelcat. no.alumina ncat. no.alumina bcat. no.alumina acat. no.florisilcat. no.500 grams 79050 33050 34050 35050 9605024PHONE: 800.441.7540 • 302.737.6960 FAX: 302.737.7115WWW.ANALTECH.COM

XTRX ColumnsXtrx Extraction Columns were designed and intended foruse in the extraction of analytes from liquid samples usingthe DuPont PREP Automated Sample Processor. Althoughthe DuPont Prep system is still available for purchase, thesecolumns may also be used on a standard vacuum manifold.There are four components to each extraction column: a cap, extraction column, effluent cup, and recovery cup. Thecartridge components are nested together so that the cap and the effluent cup seal the extraction column and preserve theactivation of the resin bed. There is also a small poly ball that rests inside the column to contain the adsorbent or resin.The extraction column contains a nominal amount of adsorbent or resin (≈ 120 mg) held by a porous bedsupport. The reservoir of the extraction column has a capacity of 4 mL. The effluent cup has a capacity of 6mL and is used to collect the column effluent while the rotor of the sample processor is spinning clockwise.Typically, this is the initial sample effluent and any wash solvent.The recovery cup has a capacity of 4 mL and its function is to collect the column effluent while the rotor isspinning counterclockwise.Typically, sample components are desorbed from the resin bed with an acidic elutingsolvent and collected in the recovery cup. The cap, which is initially used to contain the adsorbent or resin, can bereused to cap the recovery cup or effluent cup.The solid phase extraction process involves a partitioning of the sample components between a moving liquid phase (bufferedaqueous sample matrix, wash solvent, or eluting solvent) and a solid stationary phase (resin bed)The concept of solid phase extraction of materials of interest frombiological matrices has a long history. There are published extractionmethods for isolating drugs of abuse, analgesics, steroids, anticholinergics,amphetamines, anticonvulsants, antimicrobials, catecholamines, and urinarymetabolites.150 columns/box (including 150 Recovery Cups)packing milligrams catalog no.AX/S 120 11-50AX 120 11-51UNIBOND C18 120 11-52SILICA GEL 120 11-53RP/W 120 11-5425

Other Bulk SilicasSilica Gel packings for Column ChromatographyFor preparative scale column chromatography and FLASH chromatography.Analtech offers silica gel in two pore sizes and particle size ranges. Thesematerials meet the same high quality and high chemical purity levels asspecified for Analtech’s High Performance Silica Gel.Analtech offers a variety of adsorbentsin bulk quantities for TLC, LC, and HPLCapplications. These materials are usedin our own manufacturing process andare guaranteed to be of the highestquality. Consider Analtech for your bulkadsorbent requirements.Silica Gel (high purity)60Å Pore: 450m2/g Surface Area150Å Pore: 300m2/g Surface Areaquantity(grams)catalog number60å Pore35-75 µm 75-150 µmcatalog number150å Pore35-75 µm 75-150 µm500 12050 13050 14050 150501,000 12100 13100 14100 151005,000 12060 13060 14060 1506025,000 12080 13080 14080 15080ErgosilErgosil is a chemically modified silica gel designed for the analysis ofergopeptine alkaloids. Ergosil will preferentially adsorb ergopeptinealkaloids such as ergotamine and ergovaline as well as pigments fromplant extracts. The alkaloids may then be successively eluted in purifiedform for direct injection into a high pressure liquid chromatograph.New ideas pass through threeperiods:-- It can’t be done.-- It probably can be done, but it’s notworth doing.-- I knew it was a good idea all along!10 micron particle size, 60Å porequantity(grams)catalogno.500 460501,000 46100Arthur C. Clarke26

Reversed Phase Packings forColumn ChromatographyAnaltech offers two types of reversed phase packings forcolumn chromatography: A C18 octadecyl bonded silicagel and a hydrocarbon impregnated silica gel.The most common reversed phase adsorbent is theoctadecyl (C18) bonded silica gel. The C18 hydrocarbonis chemically anchored to the silica gel support. Thismaterial is compatible with all aqueous and organicsolvent combinations. The carbon content is about 14%.Pore size is 150 Å. Particle size is 35-75 microns.The hydrocarbon impregnated silica gel is a column gradeequivalent of Analtech’s unique RPS UNIPLATE. Thelong chain hydrocarbon is not chemically bonded to thesilica gel but is adsorbed onto the surface. This material iscompatible with classical reversed phase eluting solventssuch as MeOH/Water and Acetonitrile/Water. Less polarorganic solvents will solubilize the hydrocarbon.The Reversed Phase Separation (RPS) column materialis ideal for prep scale up for separations established onRPS UNIPLATES. Carbon content is about 5%. Poresize is 60 Å. Particle size is 35-75 microns.35-75μm particle (bonded - 150Å, Impregnated - 60Å)quantity(grams)Cat. No.c18 bondedcat. no.rps impreg.100 78010 50010500 78050 500505,000 78060 5006010,000 78070 50070High Efficiency TLC Adsorbents (UNISIL)Analtech offers adsorbents for those who wish to preparetheir own TLC plates with adsorptive behavior similar toAnaltech Silica Gel G and GF UNIPLATES. This highefficiency Silica Gel G is a high purity material containingcalcium sulfate hemihydrate as a binder.10μm particle, 60Å porequantity(grams)Cat. No.silica gcat. no.silica gf500 30050 400501,000 30100 40100Preparative Scale HPLC Adsorbents (UNISIL)This narrow distribution 10µ silica gel is suitable forpreparative HPLC columns. Low cost makes this anattractive material when moderate efficiency is needed.10μm particle, 60Å porequantity(grams)Cat. No.silica hcat. no.silica hf500 10050 200501,000 10100 20100PHONE: 800.441.7540 • 302.737.6960 FAX: 302.737.7115WWW.ANALTECH.COM27

TLC Educational ResourcesApplied Thin-Layer Chromatographyby Elke Hahn-Deinstrop(new 2nd edition)“Thin-Layer Chromatography is such an incredibly powerful method ofanalysis that it is hardly surprising that many analysts simply cannotbelieve it. However, its incomparable analytical power is also a reasonwhy those who rely on other analytical methods fear modern thin-layerchromatography. An analyst familiar with all the details of the process,from the basic principles up to the most sophisticated techniques, isneeded to describe the state of the art of today’s TLC. Elke Hahn-Deinstrop is such an analyst, combining deep knowledge, broadknow-how and enthusiasm.”— excerpt from the Foreword.Catalog# 10-10Practice of Thin Layer Chromatographyby Joseph C. TouchstoneSince its publication over a decade ago, this book has helped countlessanalytical chemists learn how to actually perform planar, or thin layerchromatography. Using a clear, step-by-step approach, this valuable guideoutlines the procedural basics of thin layer chromatography, detailing thespecifics of each step in single chapters.Using the same methodical, hands-on format, this new Third Editionprovides users of and novices in TLC with an unprecedented look at thestate of the science in TLC. The book includes an expanded discussionof sample preparation with an enhanced look at applications. Thereis also a more detailed description of new scanners both for isotopesand densitometry, reflecting the recent advances in this area, and newchapters on bonded phases and reactions in situ. The advent of solidphase extraction devices, an entirely new development since the lastedition, is described at length. The widening use of TLC analysis inpharmaceuticals and drugs, particularly drugs of abuse is clearly detailed.Catalog# 10-0528PHONE: 800.441.7540 • 302.737.6960 FAX: 302.737.7115WWW.ANALTECH.COM

Classic TLC References(published by Marcel Dekker)Not only are these references classics, they form the perfect trio everassembled for the serious thin layer chromatographer. You will rarely needto look any further than this mini library for information on TLC theory,techniques, instrumentation, or applications. They contain a wealth ofexpertise from the practitioner point of view and provide many practicaltips on the application of TLC. The practical detail can potentially savemany hours of laboratory experimentation.Thin-Layer ChromatographyTechniques & Applications4th Edition (Fried/Sherma)Catalog# 10-08Handbook of Thin-LayerChromatography3rd Edition (New Edition)(Sherma/Fried)Catalog# 10-09Modern Thin-LayerChromatography(Grinberg)Catalog# 10-0729

Graduated Disposable Micropipettes (pkg/250)Catalog# 20-40Economical capillary micropipettes with fire polished tipsand calibration marks at 1,2,3,4, and 5 microliters. Accuracyis within 1%. Total length of each capillary is five inches foreasy handling. A 15” aspirator tube with mouthpiece and250 micropipettes are included witheach package.Non-Calibrated Micropipettes (pkg/300)Catalog# 20-13These thin-bore capillary tubes are ideal for applyingTLC samples when high accuracy and precision are notrequired. Sample volume is estimated visually. Full volumeis approximately 9µL.Plastic Utility RackCatalog# 51-00ATLC Test Dye MixturesTest Dye Mixtures assist in developing sampleapplication and chromatographic proficiency. Inaddition, they provide readily visible chromatographicpatterns for educational demonstrations. An instructionsheet included with each dye mixture identifiescomponents and recommends solvents and developingconditions.Descriptioncatalogno.Dye Mixture I (for Silica Gel) 30-01Dye Mixture II (for Cellulose) 30-02Dye Mixture III(for Reversed Phase)30-03Dye Mixture IV (for Silica Gel) 30-04Dye Mixture Set(one each of dye I-IV)30-00Rotor Test Dye (for Silica Gel) 30-00NOTE: Approx. 40cc per jarThis convenient and inexpensive plastic utility rack has 30places to hold micropipette vials or test tubes up to 16mmin diameter. It makes storing all your micropipettes andassociated applicators simple.51-00APHONE: 800.441.7540 • 302.737.6960 FAX: 302.737.7115WWW.ANALTECH.COM31

TLC AutoSpotter• Use of standard TLC syringes• Conveniently located controlswitches/knobs• Digital temperature read-out• Even drive bar movement forcomplete dispensing of all sample• Adjustable needle guide• Alternative syringe templatesavailable for use with scored andchanneled platesTLC AutoSpotterThe TLC AutoSpotter is a semi-automated device used to apply samples onThin Layer Chromatography plates. The instrument eliminates the need formanual sample application and can be used to apply up to 18 samples at atime.The unit has been designed for use with custom made TLC syringeswith blunt Teflon® tipped needles. These special needles minimizesample “creep back” and enhance reproducibility. The syringes alsofeature Teflon plunger tips to help eliminate the problem of metal-toglasscontamination. Syringes are available in 10, 25, 50, 100 and 250µlvolumes.The device can apply samples at variable rates ranging from 3 minutes(fastest speed) to 30 minutes (slowest speed). An integral heater stripruns beneath the TLC plate at the point of sample delivery to aid insolvent evaporation. By adjusting the delivery rate and temperature ofthe heater strip, the smallest possible sample zone can be obtained.Syringes must be purchased separately. The unit will operate with as fewas one syringe.There’s a common myth thatevidence speaks for itself.It doesn’t.It just sits there on the lab table, incapableof speaking.Descriptioncatalogno.TLC AUTOSPOTTER, 110V (w/o syringes) 87-30TLC AUTOSPOTTER, 230V (w/o syringes) 87-30iAutoSpotter Scored Adapter Kit(for use with scored TLC plates)AutoSpotter Channeled Adapter Kit(for use with channeled TLC plates)87-30187-30210µL Syringe (each) 87-3125µL Syringe (each) 87-3250µL Syringe (each) 87-33100µL Syringe (each) 87-34250µL Syringe (each) 87-3532PHONE: 800.441.7540 • 302.737.6960 FAX: 302.737.7115WWW.ANALTECH.COM

tlc autospotterCheck out a videodemonstration of theTLC Autospotter atwww.analtech.com33

CycloGraph & AccessoriesKey Features of theCycloGraph• Variable speed control (100-1400 RPM)CycloGraph with RAVE technology(Radially Accelerated Variable Engine)The CycloGraph system is a centrifugally accelerated device forperforming preparative thin layer radial separations. The separations occurquickly (within 20 minutes) with no need for scraping separated bands aswith traditional Thin Layer Chromatography.• Integrated 4 watt UV lamp forhands-free viewing of separations• ON/OFF switches for motor andUV lamp• Adjustable plane angle setting(0-45 degrees)• Solvent pump with associated ON/OFFpump control switchPrinciple of OperationThe compound to be separated is applied as a solution inside theadsorbent ring of the pre-cast Rotor (Analtech’s prepared consumable forthis device) by way of a solvent pump or hand held syringe. The chosensolvent mixture is then pumped (at an appropriate speed) through theadsorbent layer effectively separating the individual components as aresult of their different affinities for the layer and solvent mixture. As theindividual rings reach the outer rim of the Rotor they are spun off the edgeof the glass and collected in the special circular trough. The angle of thetrough allows the eluent to collect at the bottom and drip out the collectionport.• Ultra quiet and accurate low flowsolvent pump (0-30ml/min)• Compatible with all commonchromatography solvents includingacetic acidStandard system includes:• CycloGraph instrument• Solvent pump with tubing• Vial of Rotor Test Dye• Instruction manual• One year warrantyCheck out a videodemonstration of theCycloGraph atwww.analtech.com34

Rotors and Accessories for the CycloGraphRotors are coated with silica gel or aluminum oxide. Bothadsorbent formulations include a calcium sulfate binder forrepeated use without damage, and a fluorescent indicatorat UV254 for ease of sample location. These rotors replacethe traditionally self-made formulations. They are overcoatedand require scraping to final thickness and shape. Theywork with both the CycloGraph and older Chromatotron.Silica gel is now also available in final scraped format readyfor use. These new rotors eliminate the tiresome, dusty andnoisy process of scraping before use. Just take them out ofpackage and chromatograph!Other accessories are available. The Rotor StorageChamber holds 10 rotors securely when not in use. TestDye can be used to fine tune operational parameters. RotorMix is available for those researchers wishing to coat buffermodified formulations.Pre-Scraped Rotor Advantages:• Ready to use - just unpack and run• No more messy silica on your lab bench• No more hi-pitched scraping sound • 4 sizes available• New packaging eliminates potentialdamagesystems & accessoriescatalogno.CycloGraph Standard System 87-40CycloGraph Deluxe System(includes all from the standard system,plus 87-43, 50-05, and set of 4 Rotors)87-40dDigital Speed Monitor (for model I) 87-43Low Flow Solvent Pump 87-42Low Flow Solvent Pump(for dry solvent applications)87-42cRotor Storage Chamber (holds 10 rotors) 50-05Rotor Scraper - for 1000 micron Rotor 05-03Rotor Scraper - for 2000 micron Rotor 05-02Rotor Scraper - for 4000 micron Rotor 05-04Rotor Scraper - for 6000 micron Rotor 05-06Rotor Scraper - for 8000 micron Rotor 05-08Finishing Rotor Scraper 05-09Rotor Test Dye (≈ 40cc) 30-05Rotors and adsorbentscatalogno.2000 micron Pre-Scaped Silica Gel Rotor 02205p4000 micron Pre-Scraped Silica Gel Rotor 02206p6000 micron Pre-Scraped Silica Gel Rotor 02207p8000 micron Pre-Scaped Silica Gel Rotor 02208pSilica GF Rotor Mix (1kg) 02210Silica GF Rotor Mix (5kg) 02250PHONE: 800.441.7540 • 302.737.6960 FAX: 302.737.7115WWW.ANALTECH.COM35

Developing Chambers & AccessoriesSmaller Developing Tanks For 10 x 10 cm PlatesThese tanks are designed for the development of 10x10cm or smallerTLC Plates. The smaller size of the chambers significantly reduces theamount of solvent needed for development. The single molded tank designincludes flat ground tops, beveled for safety on the inner and outer rims,and flat ground bottoms for stability.Latch-Lid units have a unique latching device that holds the matchingground glass surfaces of the lid and tank firmly in place. Metalcomponents of the latch-lid mechanism are made of stainless steel.Thinline TanksInside Dimensions: 12cm W x 6.4cm D x 11.5cm HAdescriptioncatalog numberThinline Latch-Lid Tank Unit 70-33Thinline Tank and Lid Unit 70-36Standard TanksInside Dimensions: 12cm W x 8.6cm D x 11.5cm HdescriptionStandard Latch-Lid Tank Unitwith aluminum rackcatalog number70-25Standard Latch-Lid Tank Unit 70-29Standard Tank and Lid Unit 70-30Aluminum Rack for 70-25 or 70-30 70-28Aluminum Rack (Teflon coated)for 70-25 or 70-3070-28TBA. Tank and Lid Unit (available in Standardand Thinline versions)B. Standard Latch-Lid and Tank Unit withAluminum RackThinline tanks are 6.4 cm in depthand require only 30 to 40ml of solvent.They accommodate up to two (2)plates. Standard tanks are 8.6 cm indepth and can accommodate up tosix (6) plates when using the speciallydesigned aluminum rack. The sixplace rack is also available with aTeflon coating.ThinlineStandard36PHONE: 800.441.7540 • 302.737.6960 FAX: 302.737.7115WWW.ANALTECH.COM

Analtech’s glass TLC developing chambers provideoptimum developing conditions for a variety of TLC platesizes and shapes. Precision ground surfaces providetight seals between tanks and glass covers and permitcomplete saturation of chambers with solvent vapor foruniform development of chromatograms.70-2170-22NOTE: Each chamber is shipped with the appropriate lid.Replacement LidsdescriptionCatalog NumberFor 70-22 & 70-21 71-22For 75-52 (7.5 cm dia.) 76-52For 75-12(14.5 cm dia.)76-12For 70-30 76-30For 70-36 76-36For 70-00 (new) 71-00bFor 70-33 70-35For 70-25 & 70-29 70-27For 80-33 & 80-26 80-3475-52 75-12Developing TanksDimensionsInsideDimensions (cm)shippingweight (each)catalognumberRectangular chambersFor 20 x 20cm TLC plates 25 H x 28 W x 7.5 D 17 lbs. 70-22For 10 x 20cm TLC plates (horizontal) 13 H x 28 W x 7.5 D 9 lbs. 70-21rectangular latch-lid chambersFor 20 x 20cm TLC plates 25 H x 28 W x 7.5 D 18 lbs. 80-33For 10 x 20cm TLC plates (horizontal) 13 H x 28 W x 7.5 D 10 lbs. 80-26cylindrical chambersFor 5 x 20cm TLC plates (vertical) 21.5 H x 6.5 Dia. 4 lbs. 75-52For 10 x 20cm TLC plates (vertical) 22.5 H x 12.5 Dia. 6 lbs. 75-12Micro chambersFor 2.5 x 10cm plates (pkg of 3) 10.5 H x 4.4 Dia. 3 lbs. 70-00For 2.5 x 10cm plates (1 each) 10.5 H x 4.4 Dia. 1 lb. 70-0170-0137

Radial/Circular Developing System for HPTLCRadial development on HPTLC plates increases separationof low Rf spots and gives better resolution than with lineardevelopment. Circular development provides a rapidmeans of analyzing single samples or for scouting solventconditions in methods development.The Analtech Radial/Circular Developing System isdesigned for the development of radial and circularchromatograms on 10 x 10 cm HPTLC plates.Saturation PadsAnaltech offers improved saturation pads made from atextured paper. This provides greater surface to assist inrapid vapor saturation of a TLC chamber air space. Themajority of TLC separations are performed under saturatedconditions in large chambers to insure even solventmigration. Also, the strategic placement of pads saturatedwith solvent in the developing chamber helps assurerapid and uniform saturation of the entire atmosphere withsolvent vapor, thereby reducing edge effects.Each package contains 100 saturation pads.The Radial/Circular Developing System includesthe chamber, the base with solvent reservoir, two clamps,the spotting guide, 500 wicks of each type, andcomplete instructions.Linear Sandwich Chamber Kit for HPTLCThe Analtech Linear Sandwich Chamber Kit is designedfor linear development of 10 x 10 cm HPTLC plates in avapor unsaturated mode. The glass-backed plate forms onewall of the chamber with the adsorbent facing inward. Thesolvent reservoir requires only 6 ml of solvent.The Kit includes a one piece brace and solvent reservoir,a spacer gauge, stainless steel chamber, clamps, andinstructions for use. See Web Site for image of kit.Description (100/pkg)catalogno.TLC Saturation Pads 20 x 20 cm 81-24TLC Saturation Pads 10 x 20 cm 81-12TLC Saturation Pads 10 x 10 cm 81-06Saturation Pad Benefits• Insure uniform chamber saturation bysolvent vaporDescriptioncatalogno.Radial/Circular Developing System 60-00Hydrophobic Wicks (500 replacements) 60-01Hydrophilic Wicks (500 replacements) 60-02Linear/Sandwich Chamber Kit 60-40Linear Sandwich Adapter 1 60-55Radial/Linear Comb. Developing System 2 60-601. Converts radial/circular system to linear sandwich mode2. Includes 60-00, 60-55, 21-00 (NANOCAP Kit), and 59077 (a box of 25 HPTLCUNIPLATES).• Improve Rf reproducibility• Reduce “edge effect” on separations38

Plate Conditioning ApparatusCatalog# 85-02The Stainless Steel Plate Conditioning Apparatus includes glassdeveloping chamber, a multi-plate rack for holding up to five 20 x 20cmTLC plates, plus a special lid assembly with hooks for suspending therack above the solvent in the developing chamber. After the appropriateequilibration period, the rack can be lowered to begin developing of theTLC plates without removing the lid.The Plate Conditioning Apparatus improves reproducibility of Rf values byassuring proper equilibration before developing begins.The Plate Conditioning Apparatus is supplied as a complete packageincluding a glass developing chamber. All components may be purchasedindividually as replacement parts.Replacement parts for plate conditioning apparatusDescriptioncatalogno.Plate Conditioning Lid 85-00Multi-Plate Rack 80-01Glass Developing Chamber 70-2285-02Plate Conditioning Apparatus• Holds up to 5 plates• Lowers plates for development withoutopening chamber• Improves Rf reproducibility• Insures solvent vapor saturationMultiplate RackCatalog# 80-01Stainless steel rack holds up to five 20 x 20 cm TLC plates forsimultaneous development in the #70-22 developing chamber. Also usefulfor activating or treating TLC plates.80-01HPTLC Two-Plate RackCatalog# 80-02Stainless steel rack for use with glass developing chambers. Holds two10x10 cm, 10x20 cm, or 5x20 cm TLC plates. Short replacement shaft isincluded for use with short chamber. Rack fits in the cylindrical chambersfor 10 cm wide plates.80-02PHONE: 800.441.7540 • 302.737.6960 FAX: 302.737.7115WWW.ANALTECH.COM39

Desiccating Cabinet for TLC, Stainless SteelCatalog# 50-0350-01Featuring a carrying handle, this 20 gauge stainless steel cabinet has aremovable desiccant tray and gasketed door with positive lock door latch.This unit holds a stainless steel drying rack, Part No. 50-00 (not suppliedwith unit).Cabinet weighs 12lbs (5.4kg), and measures 25cm D x 25cm W x 30cm H(9.75’’ D x 9.75’’ W x 11.75’’ H).TLC Plate Storage CarrierCatalog# 50-0050-00This sturdy, stainless steel rack safely holds ten 20 x 20 cm TLCUNIPLATES (or a number of smaller plates at least 20 cm wide) fordrying or storage. A backstop on each set of slides prevents plates fromfalling out the rear and permits vertical mounting of plates when optimumcirculation is desired. Handle design tilts the rack when carried to preventplates from sliding. Also available for 10 x 10 cm plates (CAT# 50-10)TLC Plate Holder (plastic)Catalog# 50-0250-02Lightweight and totally immune to contamination or disintegration fromusual TLC solvents or chemicals. The sloping bottom grooves guide TLCplates to the vertical grooves which hold plates in an upright position.Holds up to 25 TLC plates of any size.Adsorbent ScraperScraping adsorbent layers is greatly improved with this steel flat-bladescraper. Blade is 13 mm wide and tapered to a sharp edge for rapidremoval of adsorbent from plate. Handle is aluminum. A good generalpurpose tool for the laboratory.Descriptioncatalogno.Adsorbent Scraper 05-00Replacement Blades (pkg/5) 05-0140PHONE: 800.441.7540 • 302.737.6960 FAX: 302.737.7115WWW.ANALTECH.COM

Visualization EquipmentTLC Reagent SprayerCatalog# 41-00A glass sprayer with a specially designed head to eliminateformation of droplets and spattering. This feature assures deliveryof a fine misty spray and ends problems from uneven discharge.Can be used with an air line or the rubber bulb supplied. Thevolume of the reagent flask is 100 ml. Replacement spray heads(Cat# 41-01) are also available.41-0041-00Analtech TLC Sprayer Kit (3 units)Catalog# 40-01A refillable sprayer powered by its own self-contained replaceablepressure unit. Can be used with all common TLC reagents. Deliversup to 16 ounces of reagent from each pressure unit. Aspirating tubeis made of polypropylene and the particulate filter is nylon. MeetsEPA requirements for approved propellants (CFC Free).Replacements for sprayer kitDescriptioncatalogno.Pressure Units (pkg/12) 40-02Reagent Jars (pkg/12) 40-0343-0040-01Spray SupportCatalog# 43-00Stainless steel rack holds TLC plate in proper position for sprayingwith visualization reagents. Self-draining, no solvent feedback.Works well in combination with Analtech’s Disposable Spray Booth(Cat# 42-00).43-00Disposable Spray Booth (pkg/5)Catalog# 42-00This spray booth is large enough to accommodate all standard sizeTLC plates. Protects other laboratory areas from contaminationby TLC visualization reagents. After use, can be disposed of inordinary waste container.42-0041

The TLC plate rests directly on the heatdistributor and is enclosed within a narrowvapor space to insure maximum density ofreagent vapors. A Pyrex® window allowsthe reaction to be followed for optimalcolor development. A tubular port permitsthe introduction or removal of vapors anddoubles as a plate removal device.90-00It is a good morning exercise for aresearch scientist to discard a pet hypothesisevery day before breakfast.Konrad Lorenz,Austrian zoologistVapor Phase Fluorescence (VPF) Visualization ChamberThe Analtech Vapor Phase Fluorescence Chamber is a new advancein the fields of qualitative and quantitative TLC. Difficult-to-detectcompounds, such as sugars, can now be located at the nanogram levelthrough chemically induced fluorescence.VPF is a general fluorogenic visualization reaction accomplished byheating the TLC plate in the presence of ammonium bicarbonate.Fluorescence is one of the most sensitive and easily quantitated TLCdetection methods.The visualization chamber is invaluable for sulfuric acid charring and otherdetection techniques requiring heat for the formation of derivatives. Thechamber is very useful for activation and evaporation of residual mobilephase.The unit consists of an aluminum block heat distributor surrounded by astainless steel frame and non-asbestos insulation. The device mounts on astandard 6.5 X 6.5 inch hot plate (included in Catalog # 90-00) and insuresrapid rise to the set temperature and even heat distribution. A thermometerextends from the aluminum core to allow accurate reading of temperaturefor reproducible analysis.DescriptionVPF Visualization Chamber(with hot plate & #90-99)VPF Visualization Chamber(without hot plate, with #90-99)catalogno.90-0090-01Hot Plate (only) 120V or 230V 90-05Ammonium Bicarbonate (50g) 90-9942

Lightweight UV CabinetThis compact, lightweight UV cabinet will accommodateTLC plates up to 20 x 20 cm. The UV lamp is removableand may be used separately, for example, for viewingfreshly sprayed plates without removing them from thefume hood.Access to the interior of the cabinet is via a flexible,dark curtain on the front of the cabinet. The contouredviewing port contains a built-in contrast-control filter whichabsorbs both UV light and “blue haze” for safe viewingwithout eye fatigue.This cabinet is available in two versions. The standardversion (Catalog #93-06) incorporates a portable lampcontaining 6 watt tubes and offers typical light intensitiesat the cabinet floor of 350 µW/cm2 at 365 nm and 420µW/cm2 at 254 nm. The economy version (Catalog #93-04) uses a portable lamp containing 4 watt tubes andproduces typical light intensities at the cabinet floor of 300µW/cm2 at 365 nm and 310 µW/cm2 at 254 nm.DescriptionLightweight Viewing Cabinet(with 6 watt lamp)Lightweight Viewing Cabinet(with 4 watt lamp)Lightweight Viewing Cabinet(no lamps included)Available in 110V or 230V.catalogno.93-0693-0493-0193-80Darkroom Viewing CabinetThe Analtech Darkroom Viewing Cabinet has twooverhead ports which accept one or two 8 wattcombination short/long wave portable UV lamps. These8 watt lamps offer typical light intensities at the cabinetfloor of 470 µW/cm2 at 365 nm and 650 µW/cm2 at 254nm. Centered between the lamp ports is an integrally UVfiltered viewing port with a plastic eye shield. Illuminationin the visible spectrum is provided by an internal whitelight source.The cabinet is of sturdy metal construction with apolyurethane coating for chemical resistance. A 5 x 15inch entry port allows access to a flat viewing surfacelarge enough to accommodate two 20 x 20 cm TLCplates. A weighted, dark felt curtain covers the openingand insures a light-tight viewing area.DescriptionDarkroom Viewing Cabinet(no lamps included)Portable UV lamp, 8 watt(combination 254/366)catalogno.93-8093-28NOTE: Other UV lamps are available in 254, 302, and 366nm wavelengths.(Available in 110V or 230V).PHONE: 800.441.7540 • 302.737.6960 FAX: 302.737.7115WWW.ANALTECH.COM43

Portable UV LampsAnaltech offers a full range of UV lamps with various combinations ofUV wavelength, intensity, size and wattage. Each UV lamp is designedfor ease of use and will provide hours of trouble-free operation. They arehighly recommended as compact and economical sources for TLC and gelvisualization.All UV lamps that Analtech sells feature a silver-anodized aluminumhousing that makes the lamps both attractive and durable. A corrosionresistant,specular-aluminum reflector optimizes UV irradiance. Theselamps come with integrally filtered or unfiltered UV tubes, available with orwithout a separate filter assembly. Unfiltered tubes provide the highest UVintensity.Listed below are the most commonly used for TLC applications. Thecombination lamps incorporate separate tubes for short and longwave illumination.Typical Peak Intensities at a 15 cm distance(partial list):4 watt combo lamp: 300 µW/cm2 at 365 nmand 310 µW/cm2 at 254 nm.6 watt combo lamp: 350 µW/cm2 at 365 nmand 390 µW/cm2 at 254 nm.8 watt combo lamp: 470 µW/cm2 at 365 nmand 500 µW/cm2 at 254 nm.For complete list of Peak Intensity Profilesrequest our UV Lamps Data Sheet.DescriptionLong wave portable uv lamp (uv-a 365nm)4-Watt (with Integrally Filtered Tube)6-Watt (with Integrally Filtered Tube)8-Watt (with Integrally Filtered Tube)catalogno.EA-140EA-160EA-18016-Watt (with 2 x 8-Watt Tubes & Filter) 93-50short wave portable uv lamp (uv-c 254nm)4-Watt (with Filter Assembly)6-Watt (with Filter Assembly)8-Watt (with Filter Assembly)EF-140CEF-160CEF-180C16-Watt (with 2 x 8-Watt Tubes & Filter) 93-51medium wave portable uv lamp (uv-b 312nm)6-Watt (with Filter Assembly)8-Watt (with Filter Assembly)EB-160CEB-180C16-Watt (with 2 x 8-Watt Tubes & Filter) 93-53combo wave portable uv lamp (uv-a & uv-c)4-Watt (2 Tubes & Filter Assembly) 93-246-Watt (2 Tubes & Filter Assembly) 93-268-Watt (2 Tubes & Filter Assembly) 93-28Accessory Stand for Lamps 93-30Additional UV lamps not shown in this catalog are available from ANALTECH.For a complete listing please visit our Web Site. Available in 110V or 230V.44PHONE: 800.441.7540 • 302.737.6960 FAX: 302.737.7115WWW.ANALTECH.COM

UV TransilluminatorsThese transilluminators are high performance units specially designedfor fluorescence analysis, including TLC plates and electrophoresis gels.They are also well suited for DNA related research.All listed transilluminators are compatible with Analtech’s documentation &densitometry systems listed on following pages. They are equipped with apowerful and highly efficient cooling fan to maximize operating intensitiesand lower filter surface temperatures for safe operation. In addition tothe cooling fan, these transilluminators also feature a 60 minute timer forautomatic unit shut-off to prevent unnecessary solarization of the filterglass.All 312nm and 365nm models are equipped with a removable, polymerbaseddiffusing screen attached below the filter that ensures superiorlight distribution and eliminates confusing light striations caused by thecontours of the tubes. Also, all transilluminators are supplied with aprotective dust cover.DescriptionLong wave transilluminator (uv-a 365nm)8" x 8" filter, automatic timer, 6 x 15 watt tubes,filter assembly, and diffusing screen8" x 16" filter, automatic timer, 6 x 15 watt tubes,filter assembly, and diffusing screenshort wave transilluminator (uv-c 254nm)8" x 8" filter, automatic timer, 6 x 15 watt tubes,and filter assembly8" x 16" filter, automatic timer, 6 x 15 watt tubes,and filter assemblymedium wave transilluminator (uv-b 312nm)8" x 8" filter ,automatic timer, 6 x 15 watt tubes,filter assembly, and diffusing screen8" x 16" filter ,automatic timer, 6 x 15 watt tubes,filter assembly, and diffusing screencatalogno.93-69TL-365R93-67TL-254RTC-312RTL-312RTypical Peak Intensities at filtersurface (partial list):All 254nm units: 8000 µW/cm2All 365nm units: 8000 µW/cm2All 312nm units: 8000 µW/cm2For complete list of Peak Intensity Profilesvisit our Web Site.Additional Transilluminators not shown in this catalog are available from ANALTECH includingunits with odd sized filters and variable intensity control (available only on 312nm units).For a complete listing please visit our Web Site. Available in 110V or 230V.45

Documentation SystemsPhotoDoc-It Imaging SystemsWith the shift towards digital imaging, old fashioned documentation systems like the Polaroid based units have been totallyreplaced by units using high resolution digital cameras. Everything from simple imaging to colony counting to full densitometryis now possible with the digital cameras moving beyong the 10 megapixel level. With no room to fit all solutions into our catalogAnaltech has listed just a few of these options. Please visit our Web Site to see the full range of digital imaging solutions wehave to offer.The PhotoDoc-It system is a compact unit that combines a digital color camera, hood, transilluminator and a color printer forlow cost prints. The optional LCD monitor mounts to the front of the hood for live preview and capture of gels or TLC plates.• Capture brilliant color images with the high resolutiondigital color camera• Save images to the memory card for transfer to yourcomputer for viewing or documentation• Instantly print images on the lab quality color photoprinterDescriptionPhotoDoc-It 55 with M-20 TransilluminatorPhotoDoc-It 55 with LM-20 TransilluminatorPhotoDoc-It 55 with M26 TransilluminatorPhotoDoc-It 55 with LM-26 TransilluminatorPhotoDoc-It 55 without TransilluminatorLCD Screen (optional), 6.4” TFT Video Monitor(12 VDC power)catalog no.97-0274-04 (100-115V)97-0274-05 (230V)97-0274-07 (100-115V)97-0274-08 (230V)97-0274-10 (100-115V)97-0274-11 (230V)97-0274-13 (100-115V)97-0274-14 (230V)97-0274-01 (100-115V)97-0274-02 (230V)98-0093-01PhotDoc-It Systems Include Digi Camera 55, Hood Enclosure, EtBr Filter,Combination Lock, Color Printer and Choice of Benchtop UV TransilluminatorPhotoDoc-ItDigi Camera 55HoodTransilluminatorFilterPrinterCombination LockSpecificationsMegapixel resolution camera*2.5” LCD screenUSB connectivity4x optical zoom lensCompact designMetal constructionHandles for portabilityFits up to 21x26cm filtersSelect from these models:M-20: 302nm UV, 20x20cm filterLM-20: 302/365nm UV, 20x20cm filterM-26: 302nm UV, 21x26cm filterLM-26: 302/365nm UV, 21x26cm filter50mm Sq. Ethidium BromideColor printer with 300x300 dpi resolution4x6” printsUSB connectivity108 prints/ink includedDim: 7 x 5 x 2.5 in (178 x 127 x 64 mm)3 digit resettable combination lock secures thecamera to the darkroom; 2 ft. cableAdditional features included with the camera allow photography capabilitiesfor other lab uses.* Camera specifications subject to change without notice. Contact TechSupport for current camera specifications.46

ChromaDoc-It TLC Imaging SystemThis brand new imaging system was designed specificallyfor TLC applications and works well with either reflectedUV light (short and long wave) or white light. TheChromaDoc-It features a 12 megapixel digital cameraplus overhead UV providing outstanding flexibility forcolor TLC documentation and imaging.System OverviewThe cabinet contains two built-in overhead UV lamps,each with a 15-watt short wave (254nm) and long wave(365nm) UV tube. The lamps are positioned equidistantfrom the photo platform below for uniform illumination.There is also an interior overhead white light provided forease of setup. The camera and image capture functionsare controlled by the Doc-It software by way of a USBconnection between the camera and your computer. Thissoftware allows capture of quality, high resolution imagesand offers many features for maximizing image output.Image Analysis SoftwareSince its release in 2003, the Doc-It 1D Analysis softwarehas been upgraded to be more compliant with 21CFRPart 11. It is designed for analysis of TLC plates, gels,and other membranes. Now, you can generate easy tointerpret quantitative data of lanes and bands as well ascreate detailed and user-configured reports showing theanalysis results. For all the details visit our Web Site atwww.analtech.com.SpecificationsDigital Camera: Color Digital Camera, 12 megapixels,resolutions from 640x480 to 2592x1944,4x optical zoom lens,UV filters and diopter, USB interface,Li-Ion rechargeable batterySoftware:Cabinet:Power:DescriptionChromaDoc-It TLC Imaging System(complete with all items listed below)Many 21CFR Part 11 compliant features;Large selection of image captureand effects settings, compatible withWindows 98, ME, 2000, and XPOverhead 254nm/365nm UV lamps andwhite light, soft rubber viewport andcontrast control filter, lightweight accesscurtain, dimensions: 19.75W x 16.5D x15.75”H (60.2 x 41.9 x 40 cm)Available in 110V and 230Vcatalogno.96-30Doc-It LS Analysis Software 96-31White Light Transilluminator 93-37Note: The white light transilluminator is not included with the ChromaDoc-It systemSystem Components• Darkroom Viewing Cabinet• 16W Combination 254/365 nmUV Lamps, built-in (qty 2)• 12 Mega Pixel Color Digital Camera• Doc-It Imaging Software• UV Filters and DiopterPHONE: 800.441.7540 • 302.737.6960 FAX: 302.737.7115WWW.ANALTECH.COM47

HPLC ColumnsSelf-Assembled Monolayers(SAM) in Separation ScienceSelf-Assembled Monolayers (SAM) aresupramolecular organizates resembling, insome respects, the well-known Langmuir-Blodgett (LB) built-up films while displayingother distinct and rather unique features.Much of the interest in SAM stems from theirpotential in a wide range of scientific andtechnological applications. The first applicationof SAM in chromatographic separation sciencewas developed at the University of Delawareby Fatunmbi and Wirth.The bonding technique allows orderedmonolayers of functional molecules to bechemically immobilized on solid substrates,such as silica and alumina. The technique ofbonding was termed “horizontal polymerization”due to the fact that there is significant Si-O-Sibridging parallel to the silica substrate. This isachieved by reaction of trifunctional silanizingagent with the silica substrate under anhydrouscondition, except for a monolayer of wateron silica. This contrasts with conventionalpolymerization of trifunctional silanes, referredto as “vertical polymerization,” where wateris deliberately added to polymerize thereagents before attachment to the surface.The key structural difference is that horizontalpolymerization provides much higher liganddensity at the silica surface boundary.HPLC ColumnsAnaltech is proud to offer, for the first time, High Pressure Liquid ChromatographyColumns. Analtech is partnering with Separation Methods Technologies - anothercompany committed to excellence in chromatography.Separation Methods Technologies (SMT) utilizes proprietary bondingtechnologies that result in bonded phase coverages that approach 100%. SMT’smethods of bonding allow the density of the functional ligands to be controlledwith appropriate spacer molecules, a novel procedure that ensures TOTALCOVERAGE and highly cross-linked polysiloxane under layer structure. Theresults are bonded phases that are well protected and that show unprecedentedresistance to both acid and base hydrolysis. Self-Assemled Monolayers (SAM)technology provides you with the widest range of column retention selectivitiesand performance benefits.HPLC Method Development - Choosing a columnThe column of choice for analytical methods development is very easy; the bestcolumn for an application is the column that gives the highest performance underthe most favorable condition desired by the end-user. Most analytes are acidic,basic or neutral. The best initial approach is to use a mid-range pH, such as pH7. The standard SMT SAM-C18 and C8 columns are the best choice for use atthis pH because they provide superior column lifetime, extremely high selectivityand resolution. Acetonitrile or methanol and water are normally the first choice formobile phase.Another option is combination of organic solvent with phosphate buffer (withbuffer range pH 6.2-8.2) or acetate buffer (with buffer range pH 3.8-5.8). Methoddevelopment optimization can continue from here by changing several factors,including mobile phase, pH, column temperature (up to 95 degrees C). SMTSAM-C18 is uniquely stable to high temperatures, a characteristic that can beused as an additional tool to improve resolution.Using a low pH mobile phase results in the best peak shapes for basiccompounds because these analytes are fully protonated and exhibit low retentionand tailing. SMT SAM C18 or C8 columns are the best choice providing longestavailable lifetime and performance at low pH.Separations at high pH region may also be the most appropriate for certaincompounds. For example, it may be possible to separate bases in their freeform – where they are not charged. Here the retention increases as the chanceof obtaining the desired selectivity improves. SMT SAMC18 column has highestligand density available and offers the best protection (up to pH 12) of the silicasubstrate from being dissolved by the strongly basic mobile phase.48PHONE: 800.441.7540 • 302.737.6960 FAX: 302.737.7115WWW.ANALTECH.COM

Introduction to SMT SAM-C18Columns and PackingsSpecialty Columns and ApplicationsSMT has a special interest in surface modification and materials engineering.When separation is difficult with conventional bonded phases, SMT assistsin method development and special column designfor new applications. SMT specialty columns includespecial columns designed for reversed phase, normalphase, and ion exchange chromatography. Thesecolumns are specially designed for companies thatare interested in having competitive advantage inseparation and surface modification. The followingspecialty columns are currently available:ColumnPAHTNTOD-IQ and OIQC12C30UreaQuickSepChiralSepMetalSepC6F5USPMicro/Narrow BoreGuardFunctionAnalysis of polyaromatic hydrocarbonsSeparation of ExplosivesPolar/nonpolar/basic compoundsNonpolar/Polar compoundsNonpolar compoundsPolar compoundsQuick screening/analysisEnantiomersMetal RemovalSeparation of TaxolsRegulated Analytical MethodsLC/MS, LC/GC, Drug ScreeningColumn GuardSMT SAM-C18 or [OD] column is usually the firstcolumn of choice for reversed-phase chromatographicseparation or method development.When compared to other columns such asa C8, C4, CN, phenyl, or an amino bondedphase, C18 is the most hydrophobic.SMT SAM-C18 column is very stable at a widepH range and high temperatures. Separationof most basic solutes is often possible withouttrifluoroacetic acid (TFA) or other mobilephase additives. SMT packings enable youto achieve a broader pH range than what isaccessible with other commercially availablepackings. SMT utilizes a novel self-assembledmonolayers technology in all its bondingchemistries to achieve maximum coverage.The technique involves pre-treatment of thesilica substrate including rigorous control ofwater molecules. A mixture of trifunctionalligands is then allowed to come in contact withthe substrate. The result is an unprecedentedhigh-density assembly of molecules on the substrate.The unique aspect of SAM is that onlya monolayer of coverage is achieved when thebonding is performed accordingly. At least oneof the ligands (e.g. C18) is functional for theseparation while the other (e.g. C1) is used asa spacer molecule, although, it too can impactcertain selectivity needed for some separation.A typical coverage achievable with SAM is 7-8ºmole/m 2 . This coverage value is equivalentto the maximum achievable coverage on anysubstrate and it is about 50% higher thanthat achievable using the most exhaustiveconventional bonding and end-cappingmethods available in the market today.49

SMT Column SeriesSMT Column SeriesSMT-SAM-C18 [OD-Series]SMT-SAM-C18 [ODL-Series]SMT SAM-C18 [Elite C18 series]SMT SAM-C8 [O series]SMT SAM-C8 [OL series]SMT SAM-C8 [Elite-C8 series]SMT MEB1 seriesSMT MEB2 seriesSMT MEB4 seriesSMT-Phen1 seriesSMT-Phen2 seriesSMT Diol1 seriesSMT-Diol2 seriesSMT-Aminopropyl [NH2]SMT-Cyanopropyl [CN]SMT-SAX seriesSMT-WAX seriesSMT-DEAE seriesSMT-SCX seriesSMT-WCX seriesSMT PAH seriesSMT-TNT seriesSMT OD-IQ seriesSMT-C30 seriesSMT-Urea seriesSMT QuickSep seriesSMT ChiralSep seriesSMT MetalSep seriesSMT -C6F5 seriesSMT Micro/Narrow Bore seriesDescriptionSMT-C18 phase with the highest functional ligand coverage confirmed with carbon analysis results of 24% carbon load.SAM-C18 phase with the lowest functional ligand coverage confirmed with carbon analysis results of 12% carbon loadSMT Elite-C18 phases is designed to have intermediate functional ligand coverage confirmed with carbon analysis results of 16% carbon loadSMT SAM-C8 phase with the highest functional ligand coverage confirmed with carbon analysis results of 12% carbon load.SMT SAM-C8 phase with the lowest functional ligand coverage confirmed with carbon analysis results of 6% carbon loaddensity of the functional ligand, octyl molecule or C8, is moderated with the proprietary spacer molecule to ensure max. coverageThe functional ligand is methyl, C1 with carbon analysis results of about 1% carbon loadThe functional ligand is ethyl, C2 with carbon analysis results of 2% carbon loadThe functional ligand is butyl, C4 with carbon analysis results of about 4% carbon loadcontains one phenyl per ligand - provides unique selectivity for aromatic compoundscontains two phenyls per ligand - ideal for the separation of proteins, peptides and other biomoleculesacid-catalyzed cleavage of 3-(2,3-epoxypropoxy) propyl as the functional ligandacid-catalyzed cleavage of 5,6-epoxyhexyl as the functional ligandrecommended for the separation of polar compounds and can be used in 3 separation modes: Normal, Weak anion exchange, and RPWhen used in reversed-phase mode, with relatively polar solvents, CN-stationary phase offers complimentary selectivity that may beunattainable with traditional reversed-phase packings such as C18 and C8silica-based Strong Anion eXchange packing developed for separation of anionic compoundssilica-based Weak Anion eXchange packing materials developed for separation of anionic compounds[Di-Ethyl-Amino-Ethyl] columns provide a unique chemically attached hydrophilic, weak anion exchange type, functional surface desirablefor the separation of many biomolecules such as proteins, nucleotides, oligonucleotides, polynucleotides, high molecular weight RNA’sand plasmid DNA’ssilica-based Strong Cation eXchange packing materials developed for separation of cationic compounds. SMT-SCX consists of chemicallyattached hydrophilic surface derivatized to form sulfonic acid functionalitysilica-based Weak Cation eXchange packing materials developed for separation of cationic compounds. SMT-WCX consists of chemicallyattached hydrophilic surface derivatized to form carboxylic acid functionalitySMT PAH1 columns consist of octadecyl functional ligands and are made with silica with proprietary pore sizespecially designed C18 column for use in the reversed-phase separation of nitroaromatic and nitroamine derivativesunique reversed phase packing material designed to have both hydrophobic and truly hydrophilic spacer ligands. The mixed-phaseconsists of a meticulously controlled mixture of hydrophobic, C18 molecules, and proprietary hydrophilic moleculescolumns consist of Triacontyl as the functional ligandcolumns consist of Ureidopropyl as the functional ligandspecially designed for rapid resolution - these columns are ideal for fast analysis, drug screening, and purificationchiral column may contain one form of an enantiomeric compound immobilized on the surface of a packing materialproprietary strong cation exchange functional ligands that are chemically attached on silica substrate using SAM techniquecolumns consist of Pentafluorophenyl as the functional ligandcolumns are nonstandard columns designed for special HPLC applications (such as LC/MS and LC/GC)50

Unique FeatureHighly versatile; strongly recommended for basic compoundsFast mass transfer and very high efficiency for the separation of highly hydrophobic moleculesModerately hydrophobic; Offers comparable carbon load as most other commercially available C18 columns and faster mass transfer than SAM OD-seriesSMT SAM-C8 columns are designed to withstand a pH range of 1-10The spacer molecules protect the substrate from aggressive pH conditions and impact unique selectivity compared with other C8 phasesThese phases are moderately hydrophobic; nevertheless, designed to tolerate usage in very aggressive pH conditions and high temperaturesThese phases are the least hydrophobic of all the MEB columnsThese phases offer medium hydrophobicity when compared with all the other MEB phasesThese phases offer the highest hydrophobicity when compared with all the other MEB phasesOffers preferential retention of aromatic compoundsideal for the separation of proteins, peptides and other biomoleculesHigh loading capability and improved sample recoveryHigh reproducibility of bonded ligand resulting in consistent separationImproved separation of polar solutes; excellent sample recovery; high loading capabilityHomogeneous CN-functional surface that permits faster equilibration than unmodified hydroxyl silica surfaceSuperior selectivity and efficiency in the separation of proteins and biomoleculesSuperior selectivity and efficiency in the separation of proteins and biomoleculesFast reequilibration and very negligible non-specific protein interaction, High density tertiary amine functional groups that provide better selectivity and recoverycompared to conventional DEAEsuperior selectivity and efficiency in the separation of proteins and biomolecules with medium to high [isoelectric point] or pH values,Superior selectivity and efficiency in the separation of proteins and biomoleculesSuperior monitoring of polyaromatic hydrocarbons (PAH) - large organic compounds produced during combustionHighly reproducible mixed bonded phase; consistent separation of analytesStronger retention of polar molecules in aqueous eluent, Reduced backpressure; the hydrophilic hybrid enhances the solvation of the bonded phase in an aqueousenvironment. Eliminates the need for ion pairing reagentscolumns offer selectivities that are much different from C18 reversed-phase columns when applied to separation of carotenoid and related compoundscolumns are specially designed for the separation of extremely basic analytes that are not retained in traditional reversed-phase columnsQuick screening for method development; QuickSep columns can be used to assess columns suitability for a particular analysisAt least three points of simultaneous interaction between the chiral phase and one analyte enantiomer, with at least one point of stereo-chemical dependencestrong selectivities toward heavy metal ions such as Copper, gold, nickel, silver, iron, etc. Applications include precious metal recovery and waste water purificationcolumns are specially designed for the separation of Taxolsgreatly enhanced sensitivity, ideal for applications in genomics and proteomicsPHONE: 800.441.7540 • 302.737.6960 FAX: 302.737.7115WWW.ANALTECH.COM51

HPLC Column Selection GuideSMT Superior ColumnSeries Equivalent Columns USP DesignationSMT-SAM-C18[OD-Series]SMT-SAM-C18[ODL-Series]SMT-SAM-C18[Elite C18 series]Luna C18; Symmetry C18; YMC ODS-AM; Xterra C18; Kromasil C18; Inersil C18Luna C18; Symmetry C18; YMC ODS-AM; Xterra C18; Kromasil C18; Inersil C18;Zorbax SB C18Luna C18; Symmetry C18; YMC ODS-AM; Xterra C18; Kromasil C18; Inersil C18SMT-SAM-C8 [O series] Luna C8; Symmetry C8; YMC-Pack C8; Xterra C8; Kromasil C8; Inertsil C8 L7SMT-SAM-C8 [OL series] Luna C8; Symmetry C8; YMC-Pack C8; Xterra C8; Kromasil C8; Inertsil C8; Zorbax SB C8 L7SMT-SAM-C8[Elite-C8 series]Luna C8; Symmetry C8; YMC-Basic, Pack C8; Xterra C8; Kromasil C8; Inertsil C8SMT-MEB1 series Luna C5, Kromasil C1 L13SMT-MEB2 seriesZorbax SB C3SMT-MEB4 series Kromasil C4; Zorbax C4 L26SMT-Phen1 series Luna Phenyl; BetaSil Phenyl L11SMT-Phen2 series YMC-Pack Phenyl; Zorbax Phenyl L11SMT-Diol1 series Lichrosorb Diol; Spherex Diol L20SMT-Diol2 series YMC-Pack Diol; NucleoSil Diol L20SMT-Aminopropyl [NH2] Luna NH2; Kromasil NH2; YMC-Pack-NH2 L8SMT-Cyanopropyl [CN] Luna CN; Zorbax SB CN; YMC-Pack CN L10SMT-SAX series Hypersil SAX; Vydac SAX; PureGel SAX L12; L14SMT-WAX seriesSMT-DEAE seriesVydac WAX; BioSep DEAEBioSep DEAE; TSK DEAESMT-SCX series Vydac SCX; PureGel SCX; Capcell Pak SCX L9SMT-WCX seriesSMT PAH seriesSMT QuickSep seriesSMT -C6F5 seriesSMT-Silica seriesPartiSphere WCX; Gammabond WCXLuna C18; EnviroSep PAH; Vydac PAHLuna C18; C8, C4, etc.; Symmetry C18, C8, etc.; Inertsil C18, C8, etc.Phenomenex-Curosil; SupelcoSil LC-FL1L1L1L7L3SMT Manufactures Ion Exchange and Specialty ColumnsSuch As:Aminopropyl (NH2) columns, Cyanopropyl (CN) columns,DIOLS (OH) columns, Phenyl columns, DEAE columns, SAXcolumns, WAX columns, SCX columns, WCX columns, TSXcolumsSMT manufactures columns for USP methodsincluding:UPSL7 and USPL1Also available as L3, L8, L9, L10, L11, L13, L14, L20, L26,L27, and other unclassified phases, such as C12 and C30.52PHONE: 800.441.7540 • 302.737.6960 FAX: 302.737.7115WWW.ANALTECH.COM