Confined Production Processes for Non-Food Corn - Seed Science ...

• Properly document test results• Develop event specific detection method forevent identificationD. Greenhouse Propagation of Transformant• Use trained personnel for greenhouse process• Develop increase plan for each transformant• Use dedicated greenhouse space fortransplanting transformant• Implement containment steps in greenhouseappropriate for hazard, including pollen• Print labels based in plan, including loop (plant) labels.• Maintain identity of each individual plant in greenhousewith labels• Implement selfing and crossing strategy withpollen control• Maintain identity of seeds produced from crosseswith labels• Implement clean harvesting strategy with trackinginformation and labels• Clearly document whole process withtracking information• Document disposition of PMP materials• Confirm identity of transformant• Confirm transformant meets biosafety quality criteriaII. NURSERIESA. Nursery Management Planning• Assign staff with appropriate training and experience• Prepare detailed nursery plan consistent withcompliance best management standards• Achieve appropriate resource planning• Acquire necessary regulatory approvals• Audit for regulatory trainingB. Nursery Management1. Inventory Preparation (Preplanting)• Use trained personnel for preplant processesand cleaning• Use approved procedures for preplant processesand cleaning• Use dedicated or clean equipment forpreplant processes• Label, assemble, and fill planting envelopes consistentwith inventory records and approved procedures• Arrange planting envelopes in planting orderand arrange into planting boxes• Conduct field selection for appropriate temporaland spatial isolation in accordance with fieldhistories and neighbor planting plans• Prepare field for planting• Affirm necessary regulatory approvals andauthorizations are in place• Confirmation site coordinates with properranges and row labels• Prepare plot labels• Confirmation overall preplant process compliance2. Planting• Use trained personnel for planting processesand cleaning• Use approved procedures for planting processesand cleaning• Use dedicated or clean equipment forplanting processes• Check that crop is planted in accordancewith the nursery plan• Plant buffer or non-PMP pollinators• Assure appropriate labels, signage, and records,including adjustments for planting errors• Confirmation overall plantingoperations compliance• Prepare and submit regulatory documentation3. Stand management (preanthesis)• Use trained personnel for preanthesis processesand cleaning• Use approved procedures for preanthesis processesand cleaning• Use dedicated or clean equipment forpreanthesis processes• Re-confirm that temporal and spatial isolationstandards are being met• Perform preanthesis identification of PMP plantsand ensure agreement with nursery plan• Conduct regulatory/event testing in accordancewith nursery plan• Confirm overall preanthesis process compliance4. Pollen management (anthesis)• Use trained personnel for pollen managementprocesses and cleaning• Use approved procedures for pollen managementprocesses and cleaning• Use dedicated or clean equipment for pollenmanagement processesSummary: Process Descriptions 75