The University of Tennessee Health Science Center

For more information about the University of Tennessee Health Science Center, visit www.utmem.edu. 

This brochure is a joint project of The University of Tennessee Health Sciences Center and The University of Memphis. 

This brochure was organized by the UT Health Science Center Offi ce of the Vice Chancellor for Research and 

produced by the UTHSC Communications and Marketing Department. 

The University of Tennessee is an EEO/AA/ Title VI/Title IX/Section 504/ADA/ADEA institution in the provision of its education 

and employment programs and services. 

E070401-009-07

Table of Contents 

Biomedical Instrumentation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 

Comparative Medicine and Laboratory Animal Care Unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 

Drosophila Core . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 

Flow Cytometry and Cell Sorting Facility . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 

Genomics and Bioinformatics Cores . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 

Imtek MicroCat/SPECT System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 

University of Memphis 

Integrated Microscopy Center, Departments of Physics, Biomedical Engineering and 

W. Harry Feinstone Center for Genomic Research and Bioinformatics . . . . . . . . . . . . . . . . . . . . . 8 

Laser Capture Microdissection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11 

Mass Spectrometry Core Laboratory . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 

Molecular Resource Center . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 

Neuroscience Imaging . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 

Public Parking at UTHSC . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16 

1

2 

Biomedical Instrumentation 

S 

ince 1948, the Biomedical Instrumentation (BMI) 

team at the University of Tennessee campus in 

Memphis has provided a diverse array of services 

to customers both inside and outside of the 

University. Over the years, the BMI team has served thousands 

of customers in the public and private 

sector throughout the Mid-South with 

services that include: Computer Repair, 

Electronic Fabrication, Mechanical 

Design and Fabrication, Equipment 

Design and Repair, Rehabilitation 

Engineering Services, and Microscope 

Repair. 

BMI has built a solid reputation for 

timeliness, expertise, value and 

reasonable pricing. The team produces 

high quality work at costs that are 

consistently below most market rates. 

In addition, their customer service 

orientation and in-depth knowledge allow 

them to generate innovative solutions that 

serve the clients’ best interests. 

Computer Repair 

� Certifi ed as authorized service 

center for Dell, Gateway and Apple 

computers 

� Services other brands of computers, 

printers and peripherals 

� Performs system, disk drive and 

memory upgrades 

� Executes new equipment setup and 

checkout and can relocate existing 

equipment 

Mechanical Design and Fabrication 

� CAD/CAM 

� Can custom design work for 

researchers 

� Experienced as a small-scale producer 

of parts 

� Has 5th Axis milling capabilities 

� Offers complete machine shop 

services 

� Three CNC milling machines 

� Performs welding, brazing and 

soldering – aluminum, stainless steel 

� Utilizes plastic welding equipment for PVC and 

polyethylene 

� Provides relocation of delicate and expensive equipment 

Laboratory Equipment Repair 

� Repairs most manufacturers’ 

equipment 

� Provides consultation, calibration 

and verifi cation of system 

parameters 

� Offers preventive maintenance 

� Performs new equipment setup and 

checkout 

Rehabilitation Engineering 

Services 

� Installs computer hardware 

adaptations 

� Performs software adaptations 

� Executes computer repair 

� Provides custom fabrication services 

� Offers experienced counselors to 

assist clients with technology 

consultations 

Microscope Repair 

� Provides cleaning service and repair 

� Maintains an available stock of 

replacement lamps 

The BMI team’s expertise, gleaned from 

years of practical experience, result in 

real savings for customers. The team can 

extend the life of existing equipment 

through repair rather than replacement. 

For additional information contact: 

Biomedical Instrumentation 

822 Beale Street 

Beale Building, Room 141 

Memphis, TN 38163 

(901) 448-5652 

(901) 448-5337 (fax) 

instrument@utmem.edu 

www.utmem.edu/BMI

The Department of Comparative Medicine and Laboratory 

Animal Care Unit provides suitable housing facilities, animal 

husbandry services, and research technical services for 

animals used in biomedical research. The program is overseen 

by veterinarians who are board certifi ed in laboratory animal 

medicine who provide veterinary and diagnostic services, 

personnel training and expertise in laboratory animal research, 

surgery, and model development. 

The Molecular Resource Center is proud to announce the 

Drosophila melanogaster transgenic core facility (DTC) in the 

department of Neurology at the University of Tennessee Health 

Science Center. This facility will be the fi rst of its kind in the 

Memphis area and will further enhance genetic approaches 

to translational research at UTHSC. The purpose of the 

Drosophila Transgenic Core (DTC) is to encourage 

investigator’s to utilize the genetic screening tools in Drosophila 

to quickly identify genetic pathways or individual genes related 

to their disease gene of interest. The primary tools of the 

DTC will be the construction of transgenic Drosophila lines 

containing the investigator’s gene of interest under the 

control of the yeast upstream activator sequence (UAS). We 

can assist these investigators in generating visible phenotypes in 

the eye, wing, larvae or other tissues using various GAL4 

drivers maintained in the lab. Using the Drosophila deletion kits 

Comparative Medicine and Laboratory 

Animal Care Unit 


Timothy Mandrell, DVM, DACLAM 

Professor and Chair 

Comparative Medicine 

(901) 448-5656 

tmandrell@utmem.edu 

www.utmem.edu/research/LACU 

Drosophila Core 

(both Bloomington and DrosDel collections) we will help 

train postdocs or graduate students from the investigator’s 

laboratory to screen for potential suppressors or enhancers of 

the phenotypes generated. Additional projects can also be 

performed on a case-by-case basis, such as the creation of lines 

for proteomic analysis, single neuron mutation analysis or 

analysis of mutants affecting the nervous system. 


Larry Reiter, Ph.D. 

Neurology 

Room 431 Johnson 

(901) 448-2635 � (901) 448-6199 (alternate) 

lreiter@utmem.edu 

http://curlyo.utmem.edu 

3

4 

Flow Cytometry and Cell Sorting Facility 

T 

he Laboratory provides access to state-of-the-art 

analytical fl ow cytometry and cell sorting for 

UTHSC and Memphis area researchers. The 

instruments available are a BD Biosciences 

FACSAria Cell Sorter, a BD Biosciences LSR II Flow Cytometer, 

and a BD Biosciences FACSCalibur Flow Cytometer. 

Who 

The Administrative/Faculty Director of the Laboratory is 

Dr. Tony Marion, Department of Molecular Sciences. The 

Director of Operations is Dan Rosson, Ph.D. Dr. Rosson is the 

contact person for access and scheduling of services provided 

by the laboratory. 

Where 

The facilities are located in Rm. 214, 

Molecular Sciences Building, 858 

Madison and Rm. 146, The Cancer 

Research Building, 19 South Manassas, 

both within the UTHSC campus. The 

LSR II fl ow cytometer and FACSAria cell 

sorter are in MSB214. The FACSCalibur 

fl ow cytometer is in Rm. 146, Cancer 

Research Building. 

How 

Individuals wishing to schedule 

training, use of either of the fl ow 

cytometers, operator-assisted fl ow 

cytometry, or cell sorting should contact 

Dr. Rosson, MSB214, 448-4279, 

drosson@utmem.edu. Researchers may 

choose to collect fl ow cytometry data 

themselves on either the FACSCalibur 

or LSR II. Only operator-assisted fl ow 

cytometry and cell sorting is accessible with the FACSAria. 

Users of the FACSCalibur and the LSR II must fi rst complete 

a two-hour training course with Dr. Rosson or provide 

documented evidence of training and complete familiarity with 

the relevant instrument’s operation. Training, access to the fl ow 

cytometers, and scheduling for cell sorting or operator-assisted 

analysis requires an account number to which all charges may 

be submitted for payment. 

When 

Cell sorting and fl ow cytometry services and facilities are 

available Monday through Friday, 9:00 a.m. – 5:00 p.m. and at 

other times by special request. 

Flow cytometric analyses available: 

� Differentiation of relative cell size and complexity for 

eukaryotic and prokaryotic cells 

� Simultaneous detection and quantifi cation of expression 

of up to 11 cell surface and/or intracellular molecules with 

fl urophore-labeled antibodies 

� Ca2+ fl ux measurement among cell subpopulations 

defi ned by multiple cell-surface markers 

� Detection and quantifi cation of GFP-labeled protein 

expression 

� Ca2+ fl ux during cell signaling among cell subpopulations 

defi ned by cell surface markers 

� DNA quantifi cation and cell cycle analysis 

� Detection and quantifi cation of 

apoptosis 

� High Throughput Screening (HTS) 

of cells or fl urophore-labeled beads 

for the detection of cytokines or 

cell signaling phosphoproteins, i.e. 

BD Cytometric Bead Array® (CBA) 

Cell sorting services available: 

� High-speed sorting of cells 

identifi ed with up to 12 cell surface 

and/or intracellular fl uorescent 

markers into four or fewer defi ned 

subpopulations 

� Detection and high-speed sorting 

of bacteria or eukaryotic subcellular 

particles 

� Sorting into microwell plates 

� Single-cell deposition into microwell 

plates or onto microscope slides 

Flow Cytometry Facility 

The fl ow cytometry facility is contained and maintained within 

the Department of Molecular Sciences, MSB214, and the 

Cancer Research Building, Rm. 146. The facility includes two 

analytical fl ow cytometers and a cell sorter. The facility 

functions as a core laboratory accessible to all PIs and research 

laboratories at UTHSC as well as other institutions and 

companies in the Memphis area and is operated on a fee-forservice 

basis. The facility is directed by Dr. Tony Marion, 

Professor, Department of Molecular Sciences and has a fulltime, 

highly trained operator, Dr. Dan Rosson. Dr. Rosson 

has many years of training and expertise in cell biology, 

particularly cancer cell biology, and fl ow cytometry. Both Drs.

Rosson and Marion are available to all users for help in 

designing and implementing protocols to implement fl ow 

cytometry and cell sorting within their research projects. 

Cell Sorter: 

FACSAria Special Order System 

(BD Biosciences) 

The cell sorter is equipped with four lasers with 12 fl uorescence 

detectors, in addition to FSC and SSC PMTs. The 0-100 mW 

488 nm blue diode laser is equipped with fi ve fl uorescence 

detectors and a SSC detector in an octagon confi guration. 

This laser is fi ltered for detection of FITC, PE, PerCP-Cy5.5, 

PE-TxRd, and PE-Cy7. The 30 mW 638 nm red diode laser 

is equipped with three fl uorescence detectors in a trigon 

confi guration, and is fi ltered for detection of APC, APC-Cy7, 

and Alexa700. The 50 mW 

405 nm violet diode laser 

has two fl uorescence 

detectors in a trigon 

confi guration, and is fi ltered 

for detection of Pacifi c Blue/ 

Cascade Blue/Alexa405 and 

AmCyan/Pacifi c Orange/ 

Alexa430. The 20 mW 

355 nm solid-state UV 

laser has two fl uorescence 

detectors in an octagon 

confi guration, and has 

upgrade capacity for Qdots. 

Detection of Hoechst, 

Indo-1 (Ca-bound and 

unbound), and DAPI can 

be accomplished using 

this laser. FSC detection 

is selectable between PMT 

(for increased sensitivity 

and detection of subcellular 

particles and bacteria < 0.2 micrometer) and diode (for 

eukaryotic cells). The instrument has two-and four-way sort 

capacity (into tubes or microtubes) with sort rates of 22,000 

events/sec in purity sort mode with predicted recovery of 80% 

and 98% purity. This sort rate allows for sorting the entire 

mononuclear cell population from a single mouse spleen in 

approximately 1 hour. 

The unit is also specially equipped with: 

� temperature controlled sample injection and cell 

collection chambers 

� biosafety level-2 aerosol containment (for sorting 

infectious samples) 

� automated cell deposition (including single cell) into 

microwell plates or onto microscope slides. 

Flow Cytometer: 

LSR II Special Option Flow Cytometer 


The LSR II fl ow cytometer is equipped with four lasers with 

eleven fl uorescence detectors, in addition to FSC and SSC 

PMTs. The 22 mW 488 nm blue laser is equipped with four 

fl uorescence detectors and a SSC PMT, in an octagon 

confi guration, and is fi ltered for detection of FITC, PE, 

PerCP-Cy5.5, PE-TxRd, and PE-Cy7. The 20 mW 638 nm red 

laser has three fl uorescence detectors (in a trigon confi guration) 

which are capable of detecting APC, APC-Cy7, and Alexa700. 

The 22 mW 405 nm violet laser has two fl uorescence detectors 

(in a trigon confi guration), that have capacity to detect Pacifi c 

Blue/Cascade blue/Alexa405, and AmCyan/Pacifi c Orange/ 

Alexa430. The 60 mW 355 nm solid-state UV laser has two 

fl uorescence detectors (in a 

trigon confi guration), 

which can detect Hoechst, 

Indo-1 (Ca-bound and 

unbound) for Ca2+-fl ux 

measurements, and DAPI. 

This instrument is 

also equipped with a 

programmable HTS (high 

throughput screening 

device) for automated 

collection of data from 96- 

384 well microplates. 

Flow Cytometer: 

FACSCalibur (Sort) 


The FACSCalibur fl ow 

cytometer is equipped with 

two lasers, a 488 nm blue 

and 635 nm red, and four 

fl uorescence detectors in 

addition to forward and side scatter. The instrument will be 

located in the recently completed Cancer Biology Building. This 

instrument has limited sorting capability but because of the 

relatively slow sort speed is used primarily for analysis. 


Dan Rosson, Ph.D. 

Flow Cytometry 

Molecular Sciences Building, Rm. 214 

858 Madison Ave. 

(901) 448-4279 

drosson@utmem.edu 

5

6 

Genomics and Bioinformatics Cores 

Transgenic/Knockout Core 

The transgenic/knockout facility at The University of 

Tennessee, Health Science Center, is equipped with all the 

necessary hoods, incubators, microscopes, micro injectors 

and micromanipulators to produce embryonic stem cells and 

transgenic and knockout mice. The facility is in operation and 

producing KO and transgenic mice and also provides advice 

and expertise to interested faculty. The facility currently has 

one Ph.D.-level, full-time staff person that generates transgenic 

and KO/chimeric mice for the University of Tennessee Health 

Science Center. 


Ioannis Dragatsis, Ph.D., Director 

Physiology 

idragats@utmem.edu 

(901) 448-3615 � (901) 448-5822 (alternate) 

The DNA Discovery Core 

This Core has three elements: 

� Service: The core will provide genome screening and 

polymorphic detection for research and education in UT 

and other institutions in Tennessee. Meanwhile, the core 

will continue develop and modify protocols for faster 

processes and lower costs during services. The Center has 

purchased a SpectruMedix genetic analysis machine to aid 

in the detection of genetic abnormalities. 

� Research: The core serves as a resource for the 

development of research projects that involves in genetic 

mapping, fi ne mapping, genome screening, DNA 

sequencing, and positional cloning. 

� Education: The core will serve as an education and 

training base for genetic and genomic analyses. Technique 

training currently includes genomic analysis and genome 

comparison, genome screening, simple sequence repeat 

length polymorphism (SSLP) analysis, Single nucleotide 

polymorphism (SNP) detection, and DNA sequencing. 


Weikuan Gu, Ph.D., Director 

Orthopaedic Surgery 

wgu@utmem.edu 

(901) 448-2259 � (901) 448-5880 (alternate) 

Mouse Resource Core for Cancer Genetics 

The aims of this Core include: 

� Maintain and distribute to our researchers a wide variety 

of common strains of mice that have been critical in 

cancer biology. 

� Generate novel mouse strains as part of the Complex 

Trait Consortium. These lines will be distributed to the 

cancer biologist. 

� Generate new mutants in key genes known to be involved 

in cancer progression, surveillance, metastasis using novel 

methods developed at ORNL and UTHSC. 

� Core facilities for genotype verifi cation and quality 

assurance. 

� Core mouse bioinformatics module to allow investigators 

simple tools to track mouse breeding and use in 

experimental protocols. 


Robert Williams, Ph.D., Director 

Anatomy and Neurobiology 

rwilliam@nb.utmem.edu 

(901) 448-7050 � (901) 448-5965 (alternate) 

Bioinformatics Core 

In addition to Dr. Cui, the Core’s faculty includes Julia Krushkal 

Ph.D. and Rongling Li, Ph.D. who have expertise in statistical 

genetics and genetic epidemiology. The Core is in the process of 

buying a Linux Beowulf cluster with at least 32 Intel Pentium 

Xeon 2.4GMHz processors. This will provide the computational 

power to explore the core problems of Bioinformatics and 

Computational Biology like genetic network modeling; protein 

structure prediction and genome-wide DNA sequence analysis. 

Researchers will have access to expert help in the analysis of 

regulatory networks from genome-wide gene expression data. 

A programmer and a systems administrator staff the Core. 

Drs. Krushkal and Li have developed and are teaching 

introductory and advanced courses in Bioinformatics. These 

would be open to Center personnel. 


Yan Cui, Ph.D., Director 

Molecular Sciences 

ycui2@utmem.edu 

(901) 448-3240 � (901) 448-6150 (alternate)

Proteomics Core 

This core helps researchers identify protein expression patterns 

that are altered in development, growth, aging and disease. 

The Center, in conjunction with the College of Medicine, has 

invested in two state of the art mass spectrometers to assist in 

the identifi cation of protein expression, a workstation for the 

visualization of protein spots, software for the determination 

of protein differences, and a robotics system for the excision 

and processing of proteins for mass spectroscopic analysis. 

Currently, the Laboratory for Protein Analysis and Proteomics 

is equipped with the following mass spectrometers: a Bruker 

Ultrafl ex matrix-assisted-laser-desorption/ionization- 

Time-of-Flight refl ector mass spectrometer (MALDI-ToFToF) 

and a Sciex API (Atmospheric Pressure Ionization) QStar DE 

electrospray mass spectrometer. The MALDI-ToFToF is the 

primary instrument for proteomics identifi cations from 

polyacrylamide. 

T 

he Imtek MicroCAT II scanner is an X-ray computed 

tomography system capable of performing 

non-invasive imaging studies of tissue specimens or 

rats or mice in vivo. Sequential scans over time can 

be done using the same animals 

thereby reducing the number 

needed per experiment. The 

imaging system with a 60 kVp 

x-ray source and 1024 x 

1024-pixel CCD detector 

provides a 50 mm diameter x 

50 mm long cylindrical fi eld 

of view (mouse), or 100 mm 

diameter x 50 mm long FOV 

for rat studies, with 50 micron 

(10% MTF) reconstructed 

image resolution. This 

translates into a six-minute 

scan of a whole mouse. The 

source can also be operated at 

a maximum voltage of 90kVp 

to provide a 10-micron focal 

spot. With this option, the 

scanner can be re-confi gured 

by the user to provide 6-10micron 

resolution (10% MTF) 

reconstructed images. Gantry includes a four-axis precision 

motion control system, vibration isolation, a fully interlocked 

FDA compliant x-ray cabinet, and a removable mouse/ 

specimen bed. The system computer is a dual processor 

The Proteomics laboratory offers customized support and 

advice for every individual investigator desiring access. The 

format of the laboratory permits users to quickly become 

advanced and heavy users of proteomics in their research. 

This didactic and practical experience gained by investigators 

in the Proteomics laboratory ensures that protein mass 

spectrometry will be used effectively and accurately as a 

powerful research tool in the College of Medicine at the 

University of Tennessee, Memphis. 


George Hilliard, Ph.D., Director 

Molecular Sciences 

ghilliard@utmem.edu 

(901) 448-6779 � (901) 448-6150 (alternate) 

www.utmem.edu/proteomics 

Imtek MicroCat/SPECT System 

All microCAT systems can be confi gured with optional high 

resolution SPECT detector heads for multi-modality studies. 

With the optional variable focus x-ray source, images down to 15 

micron resolution can be acquired in vivo. 

2 GHz Pentium IV workstation with 1 GB RAM, >100 GB 

RAID storage and an ultra-high resolution fl at-panel display. 

Data acquisition, image reconstruction, and Amira TM 

(Indeed-Software GmbH) Advanced 3D Visualization and 

Volume Modeling software 

packages are provided. 

System operation and scanning 

of specimens/animals is provided 

by trained personnel or user and 

software analyses training can be 

arranged by appointment. 

The MicroCAT system 

confi guration includes a dualhead 

single photon emission 

tomography (SPECT) data 

acquisition system so monitoring 

and distribution of radiolabeled 

compounds can be superimposed 

on MicroCT scans. Micro-SPECT 

will enable three-dimensional 

cross-sectional imaging of 

distribution of radiolabel in 

small laboratory animals or tissue 

specimens at a spatial resolution 

in the micron \range. This dual 

MicroCAT+ SPECT functionality provides high resolution, 

high sensitivity, in vivo imaging to detect and quantify 

anatomical and functional changes associated with disease 

progression, and evaluate therapeutic effi cacy. 

7

8 

Key Features: 

� Superior image quality due 

to low-noise CCD detectors 

� Unique capability to adjust 

x-ray source and detector 

positions for high resolution and 

Field of View (FOV) 

� Multiple options for high 

speed 3-D reconstruction 

� Optional high resolution SPECT 

detector for integrated SPECT/CT 

Representative Studies: 

� Skeletal biology and disease: 

provides accurate calibrated 

measures of bone mineral density 

(BMD) and other structural 

parameters, 3-D rendering of 

trabecular architecture and bone/ 

biomaterial interfaces. 

� Joint degeneration: characterizes 

anatomical changes in joint 

space, and density/architectural 

changes in subchondral bone, 

measurement of soft tissue edema. 

� Oncology: measures solid tumor 

volume and quantify metastases; 


Integrated Microscopy Center 

T 

he Integrated 

Microscopy Center is a 

fee-for-service facility 

on the University of 

Memphis main campus since 1976. 

The facility houses a variety of light 

and electron microscopes and a 

highly trained staff. 

Available equipment: 

� FEI XL30 Environmental 

Scanning Electron Microscope 

with an EDAX energy dispersive 

X-ray system 

� JEOL JEM1200EX II 

transmission electron 

microscope with an AMT 2K 

differentiates normal tissues from 

tumors through analysis of vascularity. 

� Cardiovascular and respiratory 

disease: characterizes anatomical 

differences in tissue, organ, such as 

airway structures, vessel geometry, etc. 

� Functional imaging with integrated 

microCT/SPECT: provides insight into 

biologic processes in living animals by 

imaging the deposition of labeled 

antibodies or uptake of labeled 

molecules. The disease progression as 

well as therapeutic intervention can be 

studied in a single animal. 

For more information or to 

arrange an appointment, 

contact: 

Jinsong Huang, Ph.D. 

Orthopaedic Surgery 

(901) 523-8990 ext 6444 (Laboratory) 

(901) 647-7909 (Mobile) 

jhuang4@utmem.edu 

Bottom Mount CCD Camera 

� Nikon CI Confocal Laser 

Scanning microscope with three 

laser lines, refl ectance and a 

transmitted detector 

� Nikon E800 research microscope 

with a Nikon 12 million pixel 

camera 

� Digital Instrument NanoScope 

IIIa scanning probe microscope 

and a Digital Instrument Bioscope 

for cell imaging 

� Balzers BAF 301 Freeze Fracture 

Unit 

� Balzers FSU Freeze Substitution 

Unit

� Balzers HMP 010 High Pressure Freezer 

� Equipment for thinning metal for TEM 

Our technical staff is skilled in the operation of all of our 

equipment and in preparing the samples for you. We have made 

it a policy in the IM Center to be fl exible about client use of our 

equipment and are willing to train clients at their request. 

We have a complete TEM prep lab, which has several ultra 

microtomes, including one with cryo capabilities and a Lynx 

tissue processor. We can handle both research and clinical 

electron microscopy and are profi cient in negative staining 

techniques. Our research histology lab is set up to handle a 

variety of tissue types and give special handling for diffi cult 

specimens. We do both frozen and paraffi n samples. We also 

have protocols for immunohistochemistry. 

The University of Memphis also has other facilities on campus 

that offer services. Equipment available on a fee-for-service 

basis from other departments. 


Lou Boykins 

101 Life Science Bldg 

3774 Walker Ave 

Memphis TN 38152 

(901) 678-2034 

lboykins@memphis.edu 

http://imc.memphis.edu/ 

Biomedical Engineering 

Eugene Eckstein 

(901) 678-3505 �Eckstein@memphis.edu 

� Triboscope nanoindenter – Measures hardness 

Ernö Lindner 

(901) 678-5641 � elindner@memphis.edu 

� EcoChemie Surface Plasmon Resonance ET ___ for 

protein adsorption 

� Asylum Research MFP-3D Atomic Force Microscope for 

high resolution of fl at surface 

Warren Haggard 

(901) 678-4346 � whaggrd1@memphis.edu 

� Abbott TDX (ET 328) – Antibiotic measurements 

(selected) 

Warren Haggard/ Joel Bumgardner 

� Varian Gel Permeation Chromatography ET 322 – MW 

measurements of polymers 

� Differential Scanning Calorimetry ET 316 – Tg & thermal 

properties 

� Instron Tensile Testing Machine ET 301A – Mech. 

Properties (900 lb max load) 9

10 

Physics Department 

Sanjay Mishra 

(901) 678-3115 

srmishra@memphis.edu 

� Bruker D8 Advance X-ray Diffractometer* – Analyze phase 

composition and structure of materials 

� DuPont 910 DSC** – Phase 

transition study of materials 

M. Shah Jahan 

(901) 678-2620 

mjahan@memphis.edu 

� Varian E4 ESR/EPR 

Spectrometer – Free radicals 

analyzer 

� Bruker EMX 300 ESR/EPR 

Spectrometer*** – Free 

radical analyzer 

� Varian Cary 5E UV-Vis-NIR 

Spectrophotometer – 

optical density, absorption, 

transmission, refl ection 

� Thermoluminescence 

(Harsaw-Bicron) – defects 

in solids, chemiluminescence, 

thermal activation 

energy, radiation dosimetry 

*The Bruker D8 Advance X-ray 

Diffractometer is the state of the art 

equipment to analyze phase, 

composition, and structure of 

materials. The system is capable of 

doing fast measurements on variety 

of materials such as metals, ceramic, 

organic materials, and thin fi lms. 

The system is equipped with a 

high temperature stage (Room 

temperature-1400C) and has 

powerful analysis software. 

**DuPont 910 DSC: This Differential Scanning Calorimeter system is 

capable of performing phase transition study of materials especially 

polymers and metals. It has a dynamic temperature range of RT-600C. 

***Bruker EMX system is fully automated, high-sensitive, state-of-the-art 

free-radical analyzer 

W. Harry Feinstone Center for Genomic Research 

The W. Harry Feinstone Center for Genomic Research provides 

equipment and support for all Affymetrix products using the 

Scanner 3000 with 7G upgrade. Our technical staff is skilled 

in the operation of this equipment and in data management 

and analysis. Through consultation and collaboration we can 

provide you with methods development, study design, data 

acquisition and analysis, including production of fi gures for 

publication. GeneChip applications include RNA expression, 

splice variant detection, and Chip-on-Chip assays. In addition, 

100k and 500k SNP chips support analysis of chromosome 

structure and loss of heterozygosity, as well as, genetic 

association studies. 


Bionformatics 

The Bioinformatics Program 

at the University of Memphis 

(http://cas.memphis.edu/binf/) 

is composed of over a dozen 

faculty members with a wide 

range of research interests. 

The Program consists of four 

service units: 

� Genomic and proteomic 

design and analysis 

� Database design and 

implementation 

� Gene function and pathway 

analysis 

� Meta-analysis and 

integrative statistics 

Each unit is headed by a faculty 

member who supervises 

graduate students and staff who 

are available to collaborate with 

researchers in the community. 

The Bioinformatics Center has 

access to the High Performance 

Computing facility at the 

FedEx Institute of Technology 

(http://itd.memphis.edu/ 

researchcomputing.htm). In 

addition, the Center supports 

many commercially available statistical and analytical software 

packages as well as several in-house databases and 

bioinformatic tools. 

For more information contact: 

Thomas R. Sutter, Ph.D. 

3774 Walker Ave 

Memphis TN 38152 

(901) 678-8391 

tsutter@memphis.edu 

For parking information, visit www.memphis.edu and search for 

the Zach Curlin parking garage.

L 

aser Capture Microdissection (LCM) provides a 

rapid, reliable, one-step method to obtain pure 

populations of targeted cells from specifi c 

microscopic regions for subsequent analysis. 

LCM was developed to enable 

investigators and clinicians to perform 

tissue microdissection on a routine 

basis. There are no limitations in the 

ability to amplify DNA or RNA from 

individual cells or cell clusters (e.g., 

tumors) from tissue sections, blood 

smears, or cell cultures. LCM has been 

used to explore gene expression, loss 

of heterozygosity, micro-satellite 

instability, clonality and for 

proteomics. 

The Arcturus Pixcell II LCM at the 

UTHSC facility has major advantages 

compared to other instruments in that 

1) the laser never touches the tissue 

itself, thereby avoiding possible heat 

shock and damage, 2) cells are 

captured directly onto 

an optically transparent 

cap for reliable transfer 

of every sample to the a 

ppropriate assay tube, and 

3) the accuracy of each 

step of cell capture can 

be digitally documented 

by the accompanying 

archiving software. The 

PixCell II also has a 

fl uorescence system with 

fi lter cubes for blue (Ex 

455-495 nm/Em >510 

nm), green (Ex 503-547 

nm/Em >565 nm), and red 

(Ex 590-650 nm/Em >667 

nm) wavelengths. The 

following fl uorophores 

have been shown to be 

compatible with LCM: 

Cy2, Cy3, Cy5; Alexa 488, 

532, 546, 568; FITC; Oregon Green; propidium iodide; TRITC; 

phycoerythrin; rhodamine; To-Pro-3; and, Texas Red. 

Laser Capture Microdissection 

Figure 1. Diagrammatic representation of laser 

capture microdissection steps. Reprinted from 

Arcturus 2002 Web site (www.arctur.com). 

Procedures 

Cells can only be captured from dehydrated tissue sections or 

cell samples centered on a glass slide. 

The proprietary CapSure, an optically transparent cap 

containing a thermoplastic membrane 

transfer fi lm, is placed on the slide and 

cells are dissected by the focal melting 

of the membrane through laser 

activation (fi g 1). This short, low 

power infrared laser pulse, with beam 

sizes of 7.5, 15 and 30 µm, is triggered 

with a push of a button on the joystick. 

At no time does the laser directly touch 

the tissue sample; therefore, neither the 

quality of nucleic acids and proteins 

within the sample nor cell morphology 

are compromised, and the surrounding 

tissue remains intact on the slide. 

The captured cells remain attached to 

the transfer fi lm surface on the 

CapSure cap, which is then placed 

directly into a microcentrifuge 

tube containing the 

user-identifi ed extraction 

buffer for specifi c assay(s). 

Publication quality, 

digitized images can be 

taken of the tissue section 

before and after LCM, as 

well as of the selected 

tissue retained on the 

CapSure cap, to document 

the specifi city of each 

sample (fi g. 2). 

Extraction of DNA has 

been demonstrated using 

both ethanol fi xed and 

formalin fi xed paraffi n 

embedded tissue; RNA can 

be recovered from fresh 

frozen tissue sections. 

Guidelines for amounts 

needed per sample for molecular analyses are: 10-20 cells from 

a 10-micron section has been shown to work for PCR; 50,000 

cells for 2-D gel a nalysis; and, 20,000 cells for a western blot. 

Figure 2. Digitized images of each step of laser capture microdissection. (A) Rat 

brain was fresh frozen and 7 µm cryosections were briefl y Nissl stained, then 

rapidly dehydrated and laser-captured. (B) Paraffi n-embedded rat kidney 10 µm 

sections were briefl y stained with H&E, then rapidly dehydrated and microdissected. 

The last panel for each tissue is an image of the captured neurons (A) or 

glomerulus (B) adhered to the polymer transfer fi lm on the cap. 

11

12 

Since slide preparation is the most critical component of successful 

laser capture, the Director of the LCM facility, 

Dr. Shannon Matta, will work closely with each investigator to 

develop optimal procedure(s) for tissue preparation specifi c to 

the sample. In addition, every scientist, physician or pathologist 

who wishes to use the LCM system must fi rst be trained and 

certifi ed by Dr. Matta. 

Mass Spectrometry Core Laboratory 

T 

he new UTHSC Mass Spectrometry Core 

Laboratory will: (a) provide to funded UTHSC 

investigators state-of-the-art analytical 

methodologies; (c) train UTHSC research 

personnel; and (d) promote biological mass 

spectrometry to the UTHSC research community and other 

institutions. The center of this new UTHSC Core is the Charles 

B. Stout Neuroscience Mass Spectrometry Laboratory. The 

laboratory, headed by Dr. Dominic Desiderio, is an established 

university, national, and international mass spectrometry center 

that has conducted independent and collaborative research, 

and has provided mass 

spectrometry service to 

scientists at UTHSC and 

neighboring institutions for 

28 years. The Associate 

Directors are Dr. Giorgianni, 

Ph.D., Assistant Professor in the 

Department of Neurology, and 

Sarka Beranova-Giorgianni, 

Ph.D., Assistant Professor of 

Pharmaceutical Sciences. A 

Technologist with expertise in 

mass spectrometry will conduct 

protein identifi cation/ 

characterization experiments 

that include mass spectrometry 

measurements, database searches, and data 

interpretation. This MS Core will provide identifi cation of 

proteins from 2D gel spots or from bands from 1D gels via 

MS/MS and searches of protein sequence databases. 

Instrumentation 

� Matrix-assisted laser desorption/ionization (MALDI) linear 

trap mass spectrometer; MALDI-LTQ (Thermoelectron) 

� Liquid chromatography-nanoelectrospray linear trap mass 

spectrometer; LC-nanoESI-LTQ (Thermoelectron) 

For additional information, contact: 

Shannon Matta, Ph.D., Director 

Pharmacology 

(901) 448-2874 � (901) 448-6000 (alternate) 

smatta@utmem.edu 

www.utmem.edu/lcm 

LCM and the PixCell II instrument details are available on the 

Arcturus Pixcell II page – www.arctur.com/research_portal/ products/ 

pixcell_main.htm 

� Liquid chromatography-nanoelectrospray ion trap mass 

spectrometer; LCQDeca (Thermoelectron) 

� Liquid chromatography-nanoelectrospray quadrupole 

time-of-fl ight (QTOF) mass spectrometer; Q-TOF2 

(Waters) 

� Matrix-assisted laser desorption/ionization time-of-fl ight 

(MALDI TOF) mass spectrometer (Applied Biosystems). 

This user-friendly instrument provides the molecular 

weight (MW) of individual tryptic peptides in an 

unseparated protein digest. 

Liquid chromatography 

Three nanoLC systems are 

interfaced with the mass 

spectrometers for online LC- 

MS/MS analyses: Surveyor 

nanoLC (Thermoelectron); 

FAMOS/SWITCHOS/Ultimate 

nanoLC (Dionex); and CapLC 

nanoLC (Waters). The Dionex 

system has multidimensional 

liquid chromatography 

(MDLC) capabilities. 

Bioinformatics 

Each mass spectrometer 

includes a computer for 

instrument control and data acquisition. In addition, there are 

four computers dedicated to database searches. Search engines 

for in-house database searches include two copies of SEQUEST 

Browser, and two copies of Bioworks 3.2. The Core will use 

free online bioinformatics resources such as MASCOT (free 

version), Expasy, and Scansite. 

The Core will provide support for currently funded 

UTHSC investigators through access to advanced MS 

instrumentation and expert assistance. The Core will help to

initiate new research projects that will be enabled by 

state-of-the-art MS technologies and expertise. 

The Core will train students, postdoctoral fellows, and residents 

in mass spectrometry. Drs. Desiderio and Beranova-Giorgianni 

teach a course on the Basic Principles of Mass Spectrometry 

in the Department of Molecular Sciences, organize discussion 

groups, and present seminars to promote mass spectrometry 

and to attract new users. 

Molecular Resource Center Services 

� DNA Sequencing 

� Genetic Analysis/Genotyping 

� Phosphor-imaging 

� Real Time PCR 

� Confocal Microscopy 

� Cell Sorting 

� Microarray Facility 

� Fluorescence Microscopy 

� Primer Express Software 

DNA Sequencing 

The MRC currently has ABI PRISM 

310 and 3130xl Genetic Analyzers 

for DNA sequencing and fragment 

analysis. The 3130s have 16 capillaries 

that operate in parallel and are fully 

automated from sample loading to 

data analysis. We currently employ 

these two instruments for sequencing 

and genotyping. The 310 is a single 

capillary instrument that can support 

most gel-based assays. It is currently 

used for fragment analysis employing 

fl uorescent markers that precede the 

ones used with the 3130xl. As part of 

our DNA sequencing services, we offer 

plasmid miniprep purifi cation for 

sequencing only. Details on 

submission of transformed bacteria 

and costs can be obtained from MRC 

personnel. 

The MRC has an ABI PRISM 7700 Sequence Detection System 

for Real Time PCR. This instrument includes a built in 9600 

thermalcycler, a laser to induce fl uorescence and real-time 

detection software. Some of the applications of the ABI PRISM 

Molecular Resource Center 

ABI PRISM 3130xl Genetic Analyzer 

Light Cycler®480; Universal Probe Library 

For additional information, contact: 

Dominic M. Desiderio, Ph.D., Director 

Francesco Giorgianni, Ph.D., Associate Director 

Sarka Beranova-Giorgianni, Ph.D., Associate Director 

Neurology 

(901) 448-6199 

7700 include real-time quantitation, SNP detection, and +/- 

assays with internal positive control. 

The ABI Primer Express software allows you to independently 

design and analyze oligo sequences for a variety of PCR and 

sequencing applications. 

Real Time PCR 

The LightCycler® 480 (LC480) 

Instrument is a rapid, thermal blockcycler 

with integrated real-time, online 

detection capabilities. Based on a 

comprehensive improvement of the 

Peltier-based block technology, the 

Instrument provides extraordinary 

well-to-well temperature homogeneity 

and maximized inter-well, inter-cycle 

reproducibility. The special arrangement 

of optical components in the 

LC480 Instrument ensures the uniform 

collection of signals across the 

plate and makes analysis independent 

of the sample position on the plate. 

The LightCycler® 480 System setup 

enables the use of all current probe 

formats (e.g., SYBR Green I, 

Hydrolysis Probes, HybProbe Probes) 

and provides the ideal solution for fast 

and precise qualitative or quantitative 

detection of nucleic acids, genotyping, 

and mutation analysis. The ability to 

freely combine fi ve excitation and six 

emission fi lters permits analysis of signals from multiple dyes 

(e.g., LightCycler® Red 610, 640, LightCycler® CYAN 500, FAM, 

VIC, HEX, Cy5, and Fluorescein) in monocolor as well as in 

multiplex assays. 

13

14 

The user-friendly LightCycler® 480 Software enables highly 

fl exible and extensive data analysis for different scientifi c needs. 

Based on the modular design of the software, the user 

can customize the system by adding 

advanced analysis modules (e.g., 

LightCycler® 480 Relative 

Quantifi cation Software, LightCycler® 

480 Genotyping Software) to the 

LightCycler® 480 Basic Software (run 

option, Tm calling option, absolute 

quantifi cation option). 

Phosphor-imaging 

The Molecular Dynamics Typhoon 

Phosphor-imager is capable of 

scanning exposed storage phosphor 

screens, fl uorescent (532nm or 633nm 

excitation), and chemiluminescent 

samples. ImageQuant software 

analysis tools can be used to 

perform area or volume quantitation 

on scanned images. DNA and protein 

fragments separated by electrophoresis 

can be analyzed using the fragment 

analysis software. 

Fluorescence Microscope 

The Axiophot Photomicroscope is 

available for either transmitted light 

or fl uorescence detection on stained 

slides. Optronics Magnafi re software 

can be used to capture and print 

images, using a digital camera, from 

bright fi eld monochrome (grayscale) 

images and color images as well as 

fl uorescent images. 

Microarray Production 

Production of microarrays is offered 

DNA for the arrays can be produced 

as either amplifi ed cDNA clones or 

oligonucleotides and applied to glass 

substrates. Users must supply the appropriate 

cDNA clones or sequences for 

the oligonucleotides. Software is 

available to aid in the design of the appropriate 

oligonucleotides. Currently, a mouse 15K array from cDNA 

clones supplied by the National Institute of Aging is available. 

Agilent Bioanalyzer 

The Bioanalyzer system is an automated, computer controlled, 

integrated analyzer capable of sizing and concentration 

Zeiss Axiophot microscope 

Agilent Bioanalyer 

Liquid Handling Robot 

determinations of nucleic acids and proteins. Sample 

preparations include PCR and RT-PCR fragments, restriction 

digests, total RNA and mRNA, cell lysates, column fractions 

and purifi ed proteins. 

Liquid Handling Robot 

The MRC has excess capacity for all 

of our services. A liquid handling 

robot (Perkin Elmer Multiprobe HT 

EX) is available to scale up assays 

as necessary. 

Other MRC Services 

To better serve the needs of the research 

community, the MRC has contracted 

services with the following vendors: 

� Peptide Synthesis 

Biomolecules Midwest 

Dr. John Gorka 

1-877-939-9676 

� Protein Sequencing 

Midwest Analytical 

Dr. David McCourt 

1-800-481-0790 

Felicia Waller 

(Phosphoimaging & Digital Microscopy) 

fwaller@utmem.edu 


19 S. Manassas 3rd Floor 

Memphis, TN 38163 

(901) 448-6191 � (901) 448-8229 (fax) 

For more information on MRC, 

contact: 

William Taylor, Ph.D. 


wtaylor@utmem.edu 

Thomas Cunningham, Ph.D. 

(DNA Sequencing & Real Time PCR) 

tcunningham@utmem.edu

T 

he Neuroscience Imaging Center within the 

Neuroscience Institute/Center of Excellence, 

located on the 3rd fl oor of the Link Building at 

855 Monroe, offers use of equipment and 

technical services in the areas of transmission electron 

microscopy, confocal microscopy, 3-dimensional neuron 

tracing and mapping, conventional light microscopy 

applications and tissue preparation to all investigators in the 

medical and research fi elds in the mid-south area. 

The JEOL 2000EX transmission electron microscope 

normally runs at 60kV, but can run up to 200kV to 

examine semi-thin sections. It is equipped with a highresolution 

monitor and Hamamatsu Orca HR digital camera, 

providing portable, digital images at high resolution, in several 

formats, obviating the need for fi lm and darkroom work. 

The MicrobrightField, Inc. Neurolucida software allows 

3D reconstruction of neurons, serial reconstruction of 

brain sections, and precise cell plotting of histological or 

vibrotomed serial sections, using a motorized stage with X, Y 

and Z encoders. 

The BioRad MRC 1024 confocal imaging system is equipped 

with a Krypton/Argon laser, creating three excitation lines 

co-aligned at 488 nm (blue), 568 nm (green), and 647 nm (far 

red), enabling the user to effectively employ double or triple 

labeling techniques, and with appropriate barrier fi lters, can 

minimize bleed-through. Refl ected light and transmitted light 

image capture is also available. Image fi les can be saved to CDs 

for portability. This system uses the upright Olympus BX50 

with objective lenses ranging from 2x to 100x, and a 20x water 

immersion lens. 

Technical assistance is available with over 35 years experience in 

light and transmission electron microscopy. This lab can 

process many types of tissue, tissue slices, tissue cultures, and 

pellets, embedding in Spurr’s resin, Epon, or Epon/Araldite 

blends, as well as LRWhite, utilizing a variety of embedment 

methods, such as Beem or gelatin capsules, fl at molds, and 

fl at embedment of tissue slices. Negative staining can be 

done as well. 

For information concerning scheduling and prices, 

contact: 

Kathy Troughton 

(901) 448-5976 

www.utmem.edu/neuroscience/imaging-center 

Neuroscience Imaging 

Immunogold localization of neurophysins in dense core granules of 

vasopressin and oxytocin nerve terminals. 

Visualizing the spatial overlap of developing pathways in 

embryonic mouse brain using fl uorescent dyes and confocal laser 

scanning microscopy. 

15

16 

Public Parking Locations at the University of Tennessee 

There are fi ve public parking lots on the UTHSC campus: 

outdoor lots O and V and indoor garages E, H and P. 

Directions 

O lot is the parking lot located on the south side of the Dunn 

Dental Building at 875 Union Ave. The parking lot is 

accessible from Union Avenue, X lot and T lot. In order to gain 

entrance to the parking lot, drivers must enter the gated lot by 

pulling a ticket from the parking booth that is monitored by a 

parking attendant from the hours of 9 a.m. until 5 p.m. 

V lot is a metered parking location at the corner of Madison 

and Manassas with a physical address of 780 Madison Ave. The 

entrance to V lot is accessible from Manassas Street. 

E garage is located on the north side of the General Education 

Building, which is on the southeast corner of Dunlap and 

Madison. The physical address of E garage is 869 Madison Ave. 

The entrance to the garage is accessible from Madison Avenue. 

H garage is located on the east side of the Plaza Complex at 

930 Madison Ave. It is adjacent to the building. The garage has 

entrances from both Madison Avenue and Court Street. 

P garage is the parking garage adjacent to the Doctor’s Building 

located at 66 North Pauline. The entrance to the garage is 

accessible from Pauline Street. 

Metered Parking 

Public spaces adjacent to parking meters are located on: 

� the west side of the Student Alumni Center, 

� Manassas between Madison and Jefferson, 

� Dunlap across the street from the Hyman Administration 

Building. 

Map Buildings Legend 

4 740 Court Building D5 

34 910 Madison Building J6 



16 Alexander Bulding H6 

11 Beale Building G9 

3 Boling Center for C4 

Developmental Disabilities 

6 Cancer Research Bldg. D7 

30 Coleman College of 

Medicine Bldg. J4 

24 Crowe Research Bldg. H8 

29 Docs Field Pavilion I11 

37 Doctors Offi ce Bldg L4 

17 Feurt Pharmacy G7 

Research Bldg 

15 GEB Garage H6 

14 General Education Bldg G6 

12 Goodman Family G11 

Residence Hall 

2 Hyde Engineering B4 

23 Hyman Administration G8 

18 Johnson Building G7 

19 Link Bulding H7 

32 Madison Garage K5 

33 Madison Place Building J5 

13 Molecular Sciences Bldg G6 

21 Mooney Building H8 

22 Nash Addition I8 

25 Nash Research Bldg. I8 

36 Pauline Annex L4 

38 Pauline Garage L4 

1 Phi Chi Fraternity House B4 

28 Physical Plant Shops I10 

27 Purchasing and Physical I10 

Plant Building 

9 Randolph Hall E6 

7 Student Center Garage E5 

8 Student-Alumni Center F5 

10 Van Vleet Cancer Center F5 

5 Just Variety Building D5 

20 Wittenborg Anatomy H7 

1 

2 

3 

4 

5 

6 

7 

8 

9 

10 

11 

A B C D E F 

0 FEET 

0 

Union Avenue 

Jefferson Avenue 

MILES 

1 

2 

Madison Avenue 

Marshall Street 

500 

0. 

1 

Orleans Street 

Washington Ave. 

Adams Avenue 

3 

Monroe Avenue Extended 

Court Avenue 

© 2007 UTCSL 

This map may not be reproduced, copied, altered, or distributed by electronic or traditional means 

without permission of The University of Tennessee Cartographic Services Laboratory. 

Custom versions can be generated by calling (865) 974-5348. 

Cartography by Will Fontanez, Director 

Forrest Park 

A B C D E F 

4 

 

5 

6 

Memphis an 

Shelby Coun 

Health Depart 

7 

Jefferson Avenu 

9 

Speech 

Bldg. 

8 

Union Avenue 

Walnut Street 

Manassas Street

Health Science Center 

d 

ty 

ment 

e 

Dunlap Street 

10 10 

11 11 

Beale Street 

Dunlap Street 

12 12 

G H 

Mental Health 

Institute, Memphis 

Washington Avenue 

LeBonheur Children’s 

Medical Center 

14 14 

17 17 

18 18 

23 23 

13 13 

Linden Avenue 

15 15 

Monroe Avenue 

19 19 20 20 

26 26 

G H 


16 16 

21 21 

24 24 

Adams Avenue 

Regional Medical Center 

at Memphis 

Dunlap Street 

Hospital Street 

22 22 

25 25 

Beale Street 

29 29 

I J 

State 

Mental 

Health 

Building 

Dobbs 

Research 

Institute 

28 28 

27 27 

31 31 

I J 

30 30 

33 33 

34 34 35 35 

Memphis 

Bioworks 

Foundation 

Memphis 

Bioworks 

Foundation 

East Street 

Poplar Avenue 

Mid-South 

Hospital 

Dudley Street 

32 32 

K L M N O P 

Regional 


Parking 

Memphis 

Bioworks 

Foundation 

Memphis 

Bioworks 

Foundation 

Dudley Street 

Pauline Street 

Union Avenue 

Veterans Administration 


36 36 

37 37 

BMH 

Pauline Street 

38 38 

Baptist 

Memorial 

Hospital 

Eastmoreland Avenue 

Court Avenue 

Camila Street 


Camila Street 

Baptist 

Memorial 

Hospital 

Jefferson Avenue 

Baptist 

Memorial 

Hospital 

Linden Avenue 

K L M N O P 

Somerville Street 

Waldran Boulevard 

1 

2 

3 

4 

5 

6 

7 

8 

9 

10 

11 

17

Mission 

UT Health Science Center aims to improve human health through education, research, clinical care and public service. 

The UTHSC campuses include colleges of Allied Health Sciences, Dentistry, Graduate Health Sciences, 

Medicine, Nursing and Pharmacy. Patient care, professional education and research are carried out at hospitals 

and other clinical sites across Tennessee. Endowed professorships, Research Centers of Excellence, 

and continuing relationships with research and healthcare facilities across Tennessee 

ensure that both basic science and applied research stay focused on contemporary health topics. 

University of Tennessee Health Science Center Administration 

Chancellor (Interim) 

Hershel P. Wall, M.D. 

Chief of Staff and Executive Vice Chancellor (Interim) 

Kennard D. Brown, M.P.A., J.D. 

Deans: 

College of Allied Health Sciences (Interim) 

William R. Frey, Ph.D. Vice Chancellors: 

College of Dentistry Academic, Faculty and Student Affairs 

Russell O. Gilpatrick, D.D.S. Cheryl R. Scheid, Ph.D. 

College of Graduate Health Sciences Community Affairs (Interim) 

Richard D. Peppler, Ph.D. Kennard D. Brown, M.P.A., J.D. 

College of Medicine Development and Alumni Affairs 

Executive Dean and Memphis Dean (Interim) Linda Garceau-Luis , M.B.A., M.A. 

Steve J. Schwab, M.D. Finance and Operations 

Knoxville Dean Anthony A. Ferrara, C.P.A., M.A.S. 

James J. Neutens, Ph.D. Health System Affairs 

Chattanooga Dean Michael R. Caudle, M.D. 

David C. Seaberg, M.D. Research 

College of Nursing Leonard R. Johnson, Ph.D. 

Donna Hathaway, Ph.D. 

College of Pharmacy Director, Communications and Marketing 

Dick R. Gourley, Pharm.D. Sheila Champlin, M.A.

The University of Tennessee Health Science Center

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