CHEM 732 Fall 2011: Advances in Macromolecular Structure Two ...

CHEM 732 Syllabus, page 3Why study enzymes?Topics & ReadingspH and the Henderson-Hasselbalch Equation. Any biochemistry text.Steady State Enzyme KineticsAny biochemistry textbook, but Fersht [(1999). Structure and mechanism inprotein science: a guide to enzyme catalysis and protein folding. NewYork, Freeman] gives a nice overview.Deriving the Michaelis-Menten equationWhat do Km and kcat mean?Standard state free energies. Eyring equation. Any p-chem text.How to Review and Write a Paper: The ACS Style Guide, 2 ndeditionEnzyme ProficiencyWhat is it?Most Proficient Enzyme KnownEnzyme evolution—hypotheses and neo DarwinismLewis CA, Wolfenden R. 2008. Uroporphyrinogen decarboxylation as abenchmark for the catalytic proficiency of enzymes. Proceedings of theNational Academy of Sciences 105: 17328Stockbridge RB, Lewis CA, Yuan Y, Wolfenden R. 2010. Impact of temperatureon the time required for the establishment of primordial biochemistry, andfor the evolution of enzymes. Proceedings of the National Academy ofSciences 107: 22102False Starts at Enzyme DesignAlan Fersht: TrustAltamirano MM, Blackburn JM, Aguayo C, Fersht AR. 2000. Directed evolutionof a new catalytic activity using the α/β-barrel scaffold. Nature 403: 617Altamirano MM, Blackburn JM, Aguayo C, Fersht AR. 2002. retraction:Directed evolution of new catalytic activity using the α/β-barrel scaffold.Nature 417: 468Homme Hellinga: Error analysis & mentor-student interactionsDwyer MA, Looger LL, Hellinga HW. 2004. Computational design of abiologically active enzyme. Science 304: 1967Dwyer MA, Looger LL, Hellinga HW. 2008. Retraction. Science 319: 569Hayden EC. 2008. Chemistry: Designer debacle. Nature 453: 275