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310B. LANDRY ET AL.In order to certify that the larvae corresponded to the adults found we sequenceda fragment of the mitochondrial gene Cytochrome oxidase I (COI) of both. Wholegenomic DNA was extracted using the Nucleospin kit (Macherey-Nagel). The COIgene was amplified by PCR with two primers: k698 (5’-TACAATTTATCGCC-TAAACTTCAGCC-3’), and Pat2 (5’-TCCATTACATATAATCTGCCATATTAG-3’).The thermal profile started with an initial denaturation at 95°C for 5 min, followed by35 cycles at 94°C for 30 s, 47°C for 30 s, and 72°C for 1 min 30 s, and a final step at72°C for 10 min. The purified PCR product was sequenced in both directions usingfluorescent dye terminators in an ABI 377 automated sequencer. The sequence isavailable from GenBank (Accession No. DQ309437).In order to determine the distribution and the density of Taygete sphecophila aspredator on Polistes versicolor nests, several study sites were selected in four of thevegetation zones of Santa Cruz and Floreana Islands. In each vegetation zone a seriesof quadrats of 10 m x 10 m were made at random, and the number of active andinactive nests of Polistes versicolor were counted. The delimitation of vegetation zoneswas based on vegetation composition (Wiggins & Porter, 1971). Nests were found byvisually searching the study sites. In addition, nests found in and near Puerto Ayora, asmall town located on the littoral and arid zones on the south coast of Santa CruzIsland, were included in the study. The presence of T. sphecophila in Polistes nests wasdetermined by the presence of little holes on the back of the nests (Fig. 2) and distinctivebreaches on the capped cells normally occupied by wasp pupae. In 1999, nests ofPolistes versicolor were monitored weekly in the area of Puerto Ayora, and nests thatwere abandoned after being infested by T. sphecophila were collected during thatperiod of time. Some adults of T. sphecophila that emerged from these nests werepreserved dry for taxonomic identification. The ecological observations were madebetween April and August 1999, February and April 2002 and 2003 on Santa CruzIsland, and between April and August 1999 on Floreana Island. To test for ecologicalor insular trends in the frequency of parasitism of P. versicolor nests by T. sphecophila,we performed a G-test for goodness of fit (Sokal & Rohlf, 1995) on each island datasetusing the proportion of P. versicolor nests in a given zone to infer the expectedfrequency of parasitism by T. sphecophila.TAXONOMIC TREATMENTTaygete sphecophila (Meyrick)Epithectis sphecophila Meyrick, 1936: 624; Gaede, 1937: 113; Clarke, 1955: 290; Clarke, 1969:63, pl. 31 figs 4-4b; Makino, 1985: 25; Yamane, 1996: 85.Taygete sphecophila (Meyrick); Becker, 1984: 47; Landry, 1999: 68; Landry, 2002: 818-819.MATERIAL EXAMINED FOR MORPHOLOGICAL WORK: Moths (13 specimens from theGalapagos Islands, Ecuador): SANTA CRUZ: 1 , C[harles] D[arwin] R[esearch] S[tation], aridzone, 19.i.1989, M[ercury] V[apor] L[amp] (B. Landry); 4 (two dissected with genitalia onslides CNC MIC 4586 & BL 1196, the latter with right wings on slide BL 1313), CDRS, aridzone, 3.ii.1989, MVL (B. Landry); 2 (one dissected, slide BL 1126), Barranco, ex larva ennido Polistes versicolor, 8.ii.1999 (L. Roque, No. 99.20); 1 , NNW Bella Vista, GPS: 225 melev., S 00° 41.293’, W 090° 19.665’, 18.ii.2005, u[ltra] v[iolet] l[ight] (B. Landry, P. Schmitz);1 (dissected, slide BL 1195), 2 km W Bella Vista, 27.ii.1989, MVL (B. Landry); 1 , casa L.Roque-Albelo & V. Cruz, GPS: 137 m elev., S 00° 42.595’, W 090° 19.196’, 27.ii.2005, uvl (B.