310B. LANDRY ET AL.In order to certify that the larvae corresponded to the adults found we sequenceda fragment of the mitochondri<strong>al</strong> gene Cytochrome oxidase I (COI) of both. Wholegenomic DNA was extracted using the Nucleospin kit (Macherey-Nagel). The COIgene was amplified by PCR with two primers: k698 (5’-TACAATTTATCGCC-TAAACTTCAGCC-3’), and Pat2 (5’-TCCATTACATATAATCTGCCATATTAG-3’).The therm<strong>al</strong> profile started with an initi<strong>al</strong> denaturation at 95°C for 5 min, followed by35 cycles at 94°C for 30 s, 47°C for 30 s, and 72°C for 1 min 30 s, and a fin<strong>al</strong> step at72°C for 10 min. The purified PCR product was sequenced in both directions usingfluorescent dye terminators in an ABI 377 automated sequencer. The sequence isavailable from GenBank (Accession No. DQ309437).In order to d<strong>et</strong>ermine the distribution and the density of Tayg<strong>et</strong>e sphecophila aspredator on Polistes versicolor nests, sever<strong>al</strong> study sites were selected in four of theveg<strong>et</strong>ation zones of Santa Cruz and Floreana Islands. In each veg<strong>et</strong>ation zone a seriesof quadrats of 10 m x 10 m were made at random, and the number of active andinactive nests of Polistes versicolor were counted. The delimitation of veg<strong>et</strong>ation zoneswas based on veg<strong>et</strong>ation composition (Wiggins & Porter, 1971). Nests were found byvisu<strong>al</strong>ly searching the study sites. In addition, nests found in and near Puerto Ayora, asm<strong>al</strong>l town located on the littor<strong>al</strong> and arid zones on the south coast of Santa CruzIsland, were included in the study. The presence of T. sphecophila in Polistes nests wasd<strong>et</strong>ermined by the presence of little holes on the back of the nests (Fig. 2) and distinctivebreaches on the capped cells norm<strong>al</strong>ly occupied by wasp pupae. In 1999, nests ofPolistes versicolor were monitored weekly in the area of Puerto Ayora, and nests thatwere abandoned after being infested by T. sphecophila were collected during thatperiod of time. Some adults of T. sphecophila that emerged from these nests werepreserved dry for taxonomic identification. The ecologic<strong>al</strong> observations were madeb<strong>et</strong>ween April and August 1999, February and April 2002 and 2003 on Santa CruzIsland, and b<strong>et</strong>ween April and August 1999 on Floreana Island. To test for ecologic<strong>al</strong>or insular trends in the frequency of parasitism of P. versicolor nests by T. sphecophila,we performed a G-test for goodness of fit (Sok<strong>al</strong> & Rohlf, 1995) on each island datas<strong>et</strong>using the proportion of P. versicolor nests in a given zone to infer the expectedfrequency of parasitism by T. sphecophila.TAXONOMIC TREATMENTTayg<strong>et</strong>e sphecophila (Meyrick)Epithectis sphecophila Meyrick, 1936: 624; Gaede, 1937: 113; Clarke, 1955: 290; Clarke, 1969:63, pl. 31 figs 4-4b; Makino, 1985: 25; Yamane, 1996: 85.Tayg<strong>et</strong>e sphecophila (Meyrick); Becker, 1984: 47; <strong>Landry</strong>, 1999: 68; <strong>Landry</strong>, 2002: 818-819.MATERIAL EXAMINED FOR MORPHOLOGICAL WORK: Moths (13 specimens from theG<strong>al</strong>apagos Islands, Ecuador): SANTA CRUZ: 1 , C[harles] D[arwin] R[esearch] S[tation], aridzone, 19.i.1989, M[ercury] V[apor] L[amp] (B. <strong>Landry</strong>); 4 (two dissected with genit<strong>al</strong>ia onslides CNC MIC 4586 & BL 1196, the latter with right wings on slide BL 1313), CDRS, aridzone, 3.ii.1989, MVL (B. <strong>Landry</strong>); 2 (one dissected, slide BL 1126), Barranco, ex larva ennido Polistes versicolor, 8.ii.1999 (L. Roque, No. 99.20); 1 , NNW Bella Vista, GPS: 225 melev., S 00° 41.293’, W 090° 19.665’, 18.ii.2005, u[ltra] v[iol<strong>et</strong>] l[ight] (B. <strong>Landry</strong>, P. Schmitz);1 (dissected, slide BL 1195), 2 km W Bella Vista, 27.ii.1989, MVL (B. <strong>Landry</strong>); 1 , casa L.Roque-Albelo & V. Cruz, GPS: 137 m elev., S 00° 42.595’, W 090° 19.196’, 27.ii.2005, uvl (B.
TAYGETE SPHECOPHILA IN THE GALÁPAGOS ISLANDS311FIG. 3Tayg<strong>et</strong>e sphecophila, m<strong>al</strong>e genit<strong>al</strong>ia (sizes not proportionate). 3a, dors<strong>al</strong> view of v<strong>al</strong>vae + vinculum+ juxta and ventr<strong>al</strong> view of tegumen + uncus + gnathos d<strong>et</strong>ached on right side and spreadon left side, ph<strong>al</strong>lus removed, s<strong>et</strong>ae shown on right side only; 3b, side view of ph<strong>al</strong>lus with vesicaeverted; 3c, dors<strong>al</strong> view of ph<strong>al</strong>lus, vesica inverted, sc<strong>al</strong>e = 0.1 mm; 3d, later<strong>al</strong> view of wholegenit<strong>al</strong>ia.
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