FLUOstar OPTIMA - Labhoo.com

The multifunctional microplate reader with anexceptional combination of flexibility and performanceFLUOstar OPTIMA

FLUOstar OPTIMAFLUOstar OPTIMA - The optimal combination ofperformance and flexibility for all of your R&D applicationsWhether you need sensitivity, flexibility or extensivekinetic capabilities, the FLUOstar OPTIMA fromBMG LABTECH is the optimal solution for academicand pharmaceutical research.FlexibilityThe FLUOstar OPTIMA is a versatile microplate reader offeringfour separate reading modes with integrated resonance energytransfer detection capabilities (FRET, BRET).Fluorescence IntensityTime-Resolved FluorescenceLuminescence (Flash and Glow)Absorbance (low UV to Vis)The FLUOstar OPTIMA reads up to 384-well plates in all detectionmodes and provides 1536-well plate capability forfluorescence intensity measurements.On-board reagent injectors can be used to initiate kinetic events.Due to the individually adjustable delivery volumes for eachwell, the injectors can be used to produce dilution schemes andconcentration ranges throughout the plate. Top and bottomplate reading, precise temperature control up to 45°C (60°Cis an option), well scanning, multi-mode shaking capabilitiesand gas vent connection enhance the assay flexibility of theFLUOstar OPTIMA.simultaneously, thereby ensuring that you do not missany experimental data.The control software puts you in command over injectiontiming and pump settings such as injection speed, deliveryvolumes and the number of reagent injections per well.Kinetic data can be collected at a rapid 50 reading points persecond, or as slow as one point every 2½ hours. Furthermore,data can be collected at different rates within the sameexperiment, allowing you to monitor the signal when andwhere needed....reagent injection andfast filter switching...any plate formatOptimized for Kinetic ReactionsKinetic assays, such as Ca 2+ fluorescence measurements orflash luciferase luminescence, can be easily measured witha high degree of precision and reproducibility. Two precisionsyringe injectors have direct access to the plate measurementposition allowing reagent injection and microplate well readingHigh-Performance LuminescenceThe FLUOstar OPTIMA has been designed with a dedicatedluminescence measurement system so you do not have to makea choice between a microplate fluorometer and luminometer.The FLUOstar OPTIMA offers exceptional luminescence performancein a single instrument package that easily fulfilledPromega’s stringent DLReady (Dual Luciferase validation)criteria in 96- and 384-well plate formats.Multichromatic Detection ModesWith sixteen filter positions and features like sequential dualexcitation and sequential dual emission, the FLUOstar OPTIMAis an ideal instrument for multichromatic or ratiometric applicationssuch as FRET, BRET, 260/280 DNA quantification,

cellular indicators, calcium dyes, etc. In dual excitation applicationssuch as FURA-2, fast filter switching enables rapidmeasurement at both excitation wavelengths.Stacker and Robot CompatibilityFor medium level throughput, BMG LABTECH offers the Stackerwith an integrated barcode reader. For higher throughput, theFLUOstar OPTIMA can be integrated into many types of roboticsystems from a variety of different manufacturers.ApplicationsThe FLUOstar OPTIMA offers a unique combination offeatures to support all major assay types in many applicationareas including:Biomolecular Interaction AssaysA major field in basic research and drug discovery is themonitoring of biomolecular interactions using highly sophisticatedassays based on FRET or BRET technologiesand time-resolved fluorescence. The FLUOstar OPTIMAoffers all of these detection modes combined with on-boardreagent injection and sequential dual emission detectionfor studying receptor-ligand, protein-protein, DNA-proteinand DNA-DNA interactions.Cell-Based AssaysCell-based measurements include a large variety of assaysthat measure cell proliferation, viability, cytotoxicity, apoptosis,the analysis of second messengers like detection ofcAMP and Ca 2+ and the use of reporter gene expressionsystems. For quantifying cell-based assays, the FLUOstarOPTIMA provides all the important features like top andbottom reading (for optimal detection of cell suspensionsand adherent cells), gas venting and on-board injectors....automated platehandling with StackerControl and Evaluation SoftwareThe Windows based PC software provides an extensive rangeof options for assay design and data evaluation while beingfully compliant with FDA regulation 21 CFR Part 11. The controlsoftware offers many features including: real-time data display,unique labeling of well contents, optimization of kinetic assays,user-definable microplates, precise control of multiple injection,shaking and reading events and flexible data output optionsfor bioinformatic programs.The data evaluation part of the software is based on powerfulExcel macros and allows you to utilize any of Excel’s extensivedata manipulation features. Worksheets are provided todisplay raw data, signal plots and standard curves. Powerfulevaluation sheets are pre-programmed to perform calculationssuch as %CVs, ratios, curve fitting, EC 50 , dilution factors,etc. In addition, you can create your own workbooks for specificassays and evaluation methods.Enzyme Activity AssaysKinase, caspase and protease gene families are targetsthat play an important role in the regulation of numerousbiological processes, and have been implicated as contributorsto several diseases. The FLUOstar OPTIMA offersthe highest level of flexibility in developing enzyme activityassays and analyzing kinetic data. Four separate measurementmodes, precise temperature control, on-boardinjection, shaking, high sensitivity combined with widedynamic range and versatile kinetic software featuresallow you to accommodate a variety of enzyme assays.Quantification AssaysThe concentration of DNA, RNA and protein samples can bequantified quickly and conveniently with the FLUOstarOPTIMA. Both fluorescence-based assays and direct UVabsorbance-baseddetection are possible with the uniqueoptical system that covers a spectral range from 240 nm to740 nm (to 900 nm with extended wavelength PMT).Reporter Gene AssaysReporter genes like luciferase and GFP are extensively usedto study gene expression and associated cellular events.With the FLUOstar OPTIMA, it is also possible to use advancedco-reporter assays such as dual luciferase and FRET-basedGFP. Featuring bottom reading, well scanning, kinetics, precisetemperature control, gas venting and on-board-injection,the instrument is the perfect tool to perform reporter geneassays.

FLUOstar OPTIMA - Technical SpecificationsDue to the modularity of BMG LABTECH‘s instruments, all or combinations of the features below can be installedat purchase or upgraded at any time. Please contact your local representative for more details or a quote.Detection ModesFluorescence Intensity - including FRETLuminescence (flash and glow) - including BRETTime-Resolved Fluorescence - including DELFIA ®UV/Vis absorbanceMeasurement Modes Top and bottom readingEndpoint and Kinetic measurementsSequential Multi Excitation measurementsSequential Multi Emission measurementsRatiometric measurementsWell ScanningMicroplate Formats6 to 384-well plates, user-definableLight SourceHigh energy xenon flashlampDetectorSide window photomultiplier tubeOptical FiltersExcitation and emission filter wheels for 8 filters eachSpectral Range240 to 740 nm or 240 to 900 nmSensitivity FI < 1 fmol / well FluoresceinTRF < 70 amol/well EuropiumLUM< 30 amol/well ATPDLReady certifiedABS Dynamic range: ±0.000 - 4.000 ODReproducibility: ±0.010 OD for 0 - 2 OD rangeRead Times Flying mode: 15 s (96), 30 (384)Reagent InjectionUp to 2 built-in reagent injectorsInjection at measurement position (6 to 384-well)Individual injection volumes for each well (3 to 350 µL)Variable injection speed up to 420 µL / sUp to four injection events per wellReagent back flushingShakingLinear, orbital, and double-orbital with user-definable timeand speedGas VentSystem to inject an atmosphere or to pull a vacuum into the readerIncubation +5°C above ambient up to 45°C or 60°CSoftwareLicense-free software package including Reader Control and MARSData Analysis SoftwareDimensionsWidth: 44 cm, depth: 48 cm, height: 26 cm; weight: 26 kgStackerTHERMOstarFiltersUpgradesSensitivity is calculated according to the IUPAC standard: 3(SD blank)/slopeSpecifications are subject to change without noticeAccessoriesMagazines for up to 50 plates - continuous loading featureMicroplate Incubator and ShakerOptimized for dyes, fluorophores and specific assaysFilters for all applications from UV to NIRCustomized filters available upon requestUpgrades to include options such as additional detectionmodes, reagent injectors, extended temperature control, etc.are available. Please contact your local representative for moreinformation.HeadquartersGermanyBMG LABTECH GmbHHanns-Martin-Schleyer-Str. 1077656 OffenburgTel. +49 781 96968-0Fax +49 781 96968-67germany@bmglabtech.comAustraliaBMG LABTECH Pty. Ltd.2/24 Carbine WayMornington 3931VictoriaTel. +61 3 59734744Fax +61 3 59734711australia@bmglabtech.comFranceBMG LABTECH SARL7, Rue Roland Martin94500 Champigny s/MarneTel. +33 1 48862020Fax +33 1 48864707france@bmglabtech.comJapanBMG LABTECH JAPAN Ltd.2FT.S.-1 Building1-6-2, Shimo-cho, Omiya-kuSaitama-city 330-0844Tel. +81 48 6477217Fax +81 48 6477218japan@bmglabtech.comUKBMG LABTECH Ltd.P.O. Box 73Aylesbury Bucks HP20 2QJTel. +44 1296 336650Fax +44 1296 336651uksales@bmglabtech.comUSABMG LABTECH Inc.13000 Weston ParkwaySuite 109Cary, NC 27513Tel. +1 877 264 52 27Fax +1 919 678 16 40usa@bmglabtech.comwww.bmglabtech.com12/2009