FLUOstar OPTIMA - Labhoo.com

cellular indicators, calcium dyes, etc. In dual excitation applicationssuch as FURA-2, fast filter switching enables rapidmeasurement at both excitation wavelengths.Stacker and Robot CompatibilityFor medium level throughput, BMG LABTECH offers the Stackerwith an integrated barcode reader. For higher throughput, theFLUOstar OPTIMA can be integrated into many types of roboticsystems from a variety of different manufacturers.ApplicationsThe FLUOstar OPTIMA offers a unique combination offeatures to support all major assay types in many applicationareas including:Biomolecular Interaction AssaysA major field in basic research and drug discovery is themonitoring of biomolecular interactions using highly sophisticatedassays based on FRET or BRET technologiesand time-resolved fluorescence. The FLUOstar OPTIMAoffers all of these detection modes combined with on-boardreagent injection and sequential dual emission detectionfor studying receptor-ligand, protein-protein, DNA-proteinand DNA-DNA interactions.Cell-Based AssaysCell-based measurements include a large variety of assaysthat measure cell proliferation, viability, cytotoxicity, apoptosis,the analysis of second messengers like detection ofcAMP and Ca 2+ and the use of reporter gene expressionsystems. For quantifying cell-based assays, the FLUOstarOPTIMA provides all the important features like top andbottom reading (for optimal detection of cell suspensionsand adherent cells), gas venting and on-board injectors....automated platehandling with StackerControl and Evaluation SoftwareThe Windows based PC software provides an extensive rangeof options for assay design and data evaluation while beingfully compliant with FDA regulation 21 CFR Part 11. The controlsoftware offers many features including: real-time data display,unique labeling of well contents, optimization of kinetic assays,user-definable microplates, precise control of multiple injection,shaking and reading events and flexible data output optionsfor bioinformatic programs.The data evaluation part of the software is based on powerfulExcel macros and allows you to utilize any of Excel’s extensivedata manipulation features. Worksheets are provided todisplay raw data, signal plots and standard curves. Powerfulevaluation sheets are pre-programmed to perform calculationssuch as %CVs, ratios, curve fitting, EC 50 , dilution factors,etc. In addition, you can create your own workbooks for specificassays and evaluation methods.Enzyme Activity AssaysKinase, caspase and protease gene families are targetsthat play an important role in the regulation of numerousbiological processes, and have been implicated as contributorsto several diseases. The FLUOstar OPTIMA offersthe highest level of flexibility in developing enzyme activityassays and analyzing kinetic data. Four separate measurementmodes, precise temperature control, on-boardinjection, shaking, high sensitivity combined with widedynamic range and versatile kinetic software featuresallow you to accommodate a variety of enzyme assays.Quantification AssaysThe concentration of DNA, RNA and protein samples can bequantified quickly and conveniently with the FLUOstarOPTIMA. Both fluorescence-based assays and direct UVabsorbance-baseddetection are possible with the uniqueoptical system that covers a spectral range from 240 nm to740 nm (to 900 nm with extended wavelength PMT).Reporter Gene AssaysReporter genes like luciferase and GFP are extensively usedto study gene expression and associated cellular events.With the FLUOstar OPTIMA, it is also possible to use advancedco-reporter assays such as dual luciferase and FRET-basedGFP. Featuring bottom reading, well scanning, kinetics, precisetemperature control, gas venting and on-board-injection,the instrument is the perfect tool to perform reporter geneassays.