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Cross-hybridization<br />
expressed as a ‘homology score’<br />
Only BLAST hits that passed filtering are considered<br />
If m is the number of BLAST hits considered in position i.<br />
Let h=(h1 i ,...,hm i ) be the BLAST hits in position i in the oligo<br />
100×<br />
n −<br />
BLAST max score =<br />
Where n is the length of the oligo<br />
i<br />
∑ = 1<br />
n<br />
max( h1<br />
,..., hm )<br />
100×<br />
n<br />
BLAST hits {<br />
Max hit in pos. i<br />
i<br />
i<br />
Oligo<br />
100%<br />
0
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