Probe design

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ΔTm Distribution for oligo length intervals
Avoid self annealing oligos Sensitivity may be influenced <strong>Probe</strong>s that form strong hybrids with it self i.e. probes that fold should be avoided. But, accurate folding algorithms like the one employed by mFOLD or RNAfold, is too time consuming, for large scale folding of oligos. Time consumption: mFOLD ~2 sec / 30’mer Pr. gene (500bp) ~16 min.
Page 1 and 2: Probe design for microarrays by H.
Page 3 and 4: Probe Type comparisons PCR products
Page 5 and 6: Download OligoWiz and try the sampl
Page 7 and 8: Probe Specificity Hughes et al. 200
Page 9 and 10: Filtering Self Detecting 50 bp BLAS
Page 11 and 12: Similar Affinity for all oligos Ano
Page 13: Tm distributions for 30’mers and
Page 17 and 18: Folding a lot of oligos a fast heur
Page 19 and 20: Probes With Very Common sub sequenc
Page 21 and 22: Location of Oligo within transcript
Page 23 and 24: Export the probes to a file Export

Probe design

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