manual on the diagnosis of nipah virus infection in animals

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PageChapter 4: Confirmatory laboratory diagnosis 29 - 37- Serological tests 29- Histopathology 32- Immunohistochemistry 33- Virus isolation 35- Electron microscopy 36- PCR 37Chapter 5: Control and eradication 38 – 44- Advance planning 38- The organization of the control operation 39- Movement controls on pigs 41- Mass culling of active disease farms 41- Financial assistance 42- A national testing and surveillance programme 42- A national abattoir monitoring and testing programme 43Chapter 6: Managing pig industries for freedom fromNipah virus infection 45 - 50- Actions from the pig industry 45- Actions from governments 47- Managing the risk from the wildlife reservoir 50ii
PageIllustrationsFigure 1: World distribution of flying foxes (genus Pteropus) 9Figure 2: Protective equipment worn by those performingnecropsies 14Figure 3: Acute onset neurological signs including seizures,tetanic-like spasms and jaw champing are seen in sows 25Figure 4: Nipah virus syncytium with nuclei characteristicallyforming a ring around the periphery of themulti-nucleated cell 36Figure 5: Culling and disposal by shooting and burying 44Figure 6: Disinfection of burial sites using chlorinated lime 44References 51 - 55Appendices 56 - 761 Standard operating procedures (SOPs) for workingon suspected Nipah virus infected premises 56 - 582 Standard operating procedures (SOPs) for performingnecropsies and collecting serum samples on Nipahvirus infected premises 59 - 603 Checklist of equipment and supplies for Nipah virusfield investigations 59 - 60iii
Page 1 and 2: RAP publication no. 2002/01MANUAL O
Page 3 and 4: The designations employed and the p
Page 5 and 6: Aziz JamaludinDirector of ResearchV
Page 7: TABLE OF CONTENTSPageLists of chapt
Page 12: disease and the infective agent. It
Page 16 and 17: where unknown disease agents exist
Page 18 and 19: Epidemiological evidence suggested
Page 20 and 21: pigs from 896 farms were destroyed
Page 22 and 23: They are the largest bats in the wo
Page 24 and 25: CHAPTER TWOWORKING SAFELY WITH NIPA
Page 26 and 27: Keep the use of sharps to a minimum
Page 28 and 29: Figure 2: Protective equipment worn
Page 30 and 31: container (ideally a plastic or met
Page 32 and 33: tubes should be appropriately dress
Page 34 and 35: Triton-X100 prior to testing. Alter
Page 36 and 37: CHAPTER THREEREACHING A PRESUMPTIVE
Page 38 and 39: Clinical observations in suckling p
Page 40 and 41: The susceptibility of cats was conf
Page 42 and 43: violation of farm biosecurity shoul
Page 44 and 45: laboratories are establishing an EL
Page 46 and 47: 602 (see Appendix 6). The recipient
Page 48 and 49: ain tissue), lungs, trachea and kid
Page 50 and 51: Figure 4: Nipah virus syncytium wit
Page 52 and 53: CHAPTER FIVECONTROL AND ERADICATION
Page 54 and 55: compensation were made by the Cabin
Page 56 and 57: The pigs were culled by shooting, a
Page 58 and 59:
Figure 5: Culling and disposal by s
Page 60 and 61:
Chapter 4) will be the most efficie
Page 62 and 63:
Epidemiology is the discipline with
Page 64 and 65:
adopt methods of operation that wil
Page 66 and 67:
pp. 81-83. Jointly organized by the
Page 68 and 69:
Middleton D., Westbury H., Morrissy
Page 70 and 71:
APPENDIX 1STANDARD OPERATING PROCED
Page 72 and 73:
17. Wet any cloth overalls in disin
Page 74 and 75:
4. Instruct personnel not involved
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- plastic scrubbing brush- towels-
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SampleVirusisolationImmunohistochem
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3 LABORATOIRE P4 JEAN MERIUEXContac
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a watertight secondary packaging;ot
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area of the Shipper's Declaration:
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APPENDIX 7PACKING DIAGNOSTIC SPECIM
Page 88 and 89:
(b) an outer packaging of adequate
Page 90:
- Substances shipped in liquid nitr
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manual on the diagnosis of nipah virus infection in animals

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