Instruction for the Invisorb Spin Cell Mini Kit

Protocol 3: DNA isolation of DNA from Swab material(Buccal Swab; Vaginal Swab)Important: Incubate the Elution Buffer D at 70°C1. Incubation of the swab in a 1.5 or 2.0 ml microfuge tube with 500 Al Lysis Buffer D for 5 min.Grind the swab on the wall of the tube carefully and remove the swab.Note: Should Lysis Buffer D contain any precipitates incubate it in a 50°C waterbath for a short time!Note:Vortex the Carrier Suspension B thoroughly prior to use.2. Addition of 10 Al Carrier Suspension B and 200 Al of Binding Buffer HL, mix briefly andtransfer the suspension directly onto the RTA Spin Filter. Incubation for 2 min.3. Centrifugation for 3 min at 13.400 x g (12.000 rpm). Discard the flow-through and reuse theRTA Receiver Tube.4. Addition of 550 Al Wash Buffer and centrifugation for 1 min at 13.400 x g (12.000 rpm).Discard the flow-through.5. Repeat step 4 once again. Afterwards centrifuge for 3 min at 13.400 x g (12.000 rpm) toremove the residual ethanol.6. Transfer the RTA Spin Filter into a new 1.5 ml Receiver Tube. Add 100 Al of ElutionBuffer D or Tris buffered ddH 2 0 (pH 8.5 - 9.0) prewarmed to 70°C directly onto themembrane of the RTA Spin Filter. Incubation for 2 min. Centrifugation at 9.300 x g(10.000 rpm) for 2 min.Discard the RTA Spin Filter.Protocol 4: DNA isolation from Serum or Plasma (max. 100Fl)Important: Incubate the Elution Buffer D at 70°C1. Addition of 500 Al Lysis Buffer D to the Serum or Plasma sample (max. 100 Al) andincubation for 10 min (continuous shaking increases the lysis efficiency!).Note:Note:Should Lysis Buffer D contain any precipitates incubate it in a 50°C waterbath for a shorttime!Vortex the Carrier Suspension B thoroughly prior to use.2. Addition of 10 Al Carrier Suspension B and 200 Al Binding Buffer HL, mix briefly andtransfer the suspension directly onto the RTA Spin Filter. Incubation for 2 min.3. Centrifugation for 3 min at 13.400 x g (12.000 rpm). Discard the flow-through and reuse theRTA Receiver Tube.4. Addition of 550 Al Wash Buffer and centrifugation for 1 min at 13.400 x g (12.000 rpm).Discard the flow-through.5. Repeat step 4 once again. Afterwards centrifuge for 3 min at 13.400 x g (12.000 rpm) toremove the residual ethanol.6. Transfer the RTA Spin Filter into a new 1.5 ml Receiver Tube. Add 100 Al of ElutionBuffer D or Tris buffered ddH 2 0 (pH 8.5 - 9.0) prewarmed to 70°C directly onto themembrane of the RTA Spin Filter. IIncubation for 2 min. Centrifugation at 9.300 x g(10.000 rpm) for 2 min. Discard the RTA Spin Filter.14Invisorb ® Spin Cell Mini Kit 032010