Instruction for the Invisorb Spin Cell Mini Kit

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ContentsKit contents of the Invisorb ® Spin Cell Mini Kit ....................................................................... 3Symbols ...................................................................................................................................... 4Storage........................................................................................................................................ 4Quality control............................................................................................................................ 4Intended use ............................................................................................................................... 5Product use limitation................................................................................................................ 5Safety information...................................................................................................................... 6Product characteristics of the Invisorb ® Spin Cell Mini Kit..................................................... 7Principle and procedure ............................................................................................................ 7Sampling and storage of starting material............................................................................... 7Procedure............................................................................................................................... 8Yield and quality of genomic DNA .......................................................................................... 8Important notes....................................................................................................................... 9Important points before starting a protocol.............................................................................. 9Preparing reagents and buffers .............................................................................................. 9Reagents and equipment to be supplied by user .................................................................. 10Important indications 10Scheme of the Invisorb ® Spin Cell Mini Kit: .......................................................................... 11Protocol 1: DNA isolation from cell culture (24-Well-Plates; max. 2x10 6 cells ....................... 12Protocol 2: DNA isolation from cell culture (96-Well-Plates)................................................. 13Protocol 3: DNA isolation of DNA from swab material (buccal swab; vaginal swab).............. 14Protocol 4: DNA isolation from Serum or Plasma (max. 100Al)............................................ 14Troubleshooting....................................................................................................................... 15Appendix................................................................................................................................... 16General notes on handling DNA ........................................................................................... 16Ordering information................................................................................................................ 17Invitek distributors ................................................................................................................... 182Invisorb ® Spin Cell Mini Kit 032010
Kit contents of the Invisorb ® Cell Mini KitStore all other kit components at room temperature (RT)!3 DNAextractions10 DNAextractions50 DNAextractions250 DNAextractionsCatalogue number 1033100100 1033100900 1033100200 1033100300Lysis Buffer D 5 ml 10 ml 30 ml125 mlBinding Buffer HL 2 ml 2 x 2 ml 15 ml 60 mlCarrier Suspension B 100 Al 150 Al 600 Al 2 x 1.3 mlWash Buffer15 ml(ready to use)15 ml(ready to use)18 ml(final volume 60 ml)2 x 45 ml(final volume 2 x 150 ml)Elution Buffer D 2 ml 2 x 2 ml 15 ml 60 mlRTA Spin Filter Set 3 10 50 2501.5 ml Receiver Tubes 3 10 50 250Manual 1 1 1 1Initial stepsWarmingwaterbath orthermomixer to70°C and heat theElution Buffer Dto the sametemperature.Warmingwaterbath orthermomixer to70°C and heat theElution Buffer Dto the sametemperature.Add 42 ml of 96-100% Ethanol to thebottle Wash Bufferand mix thoroughly.Store the WashBuffer always withfirmly closed cap.Storage at roomtemperature!Add 105 ml of 96-100%Ethanol to each bottleWash Buffer and mixthoroughly.Store the Wash Bufferalways with firmly closedcap.Storage at roomtemperature!Warming waterbathor thermomixer to70°C and heat theElution Buffer D tothe sametemperature.Warming waterbath orthermomixer to 70°Cand heat the ElutionBuffer D to the sametemperature.3Invisorb ® Spin Cell Mini Kit 032010
Page 1: Instruction for the Invisorb ® Spi
Page 5 and 6: Intended useThe Invisorb ® Spin Ce
Page 7 and 8: Product characteristic of the Invis
Page 9 and 10: Important notesImportant points bef
Page 11 and 12: Scheme of the Invisorb ® Spin Cell
Page 13 and 14: Protocol 2: DNA isolation from cell
Page 15 and 16: TroubleshootingProblem Cause Commen
Page 17 and 18: Ordering informationProduct Package
Page 19: KoreaPhilekorea Technology Inc.www.

Instruction for the Invisorb Spin Cell Mini Kit

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