Instruction for the Invisorb Spin Cell Mini Kit

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Safety informationWhen working with chemicals, always wear a suitable lab coat, disposable gloves, and protectivegoggles. Heed the legal requirements for working with biological material!For more information, please consult the appropriate material safety data sheets (MSDS). Theseare available in convenient and compact PDF format online at www.invitek.de under each Invitekkit and kit component.If the buffer bottles are damaged or leaking, wear gloves and protective goggles when discardingthe bottles in order to avoid any injuries.Invitek has not tested the liquid waste generated by the Invisorb ® Spin Cell Mini procedures forresidual infectious materials. Contamination of the liquid waste with residual infectious materials ishighly unlikely, but cannot be excluded completely. Therefore, liquid waste must be consideredinfectious and be handled and discarded according to local safety regulations.Below European Community risk and safety phrases for the components of the Invisorb ® Spin CellMini Kit to which they apply, are listed.Lysis Buffer D:Binding Buffer HL:Xn contains: Guanidinthiocyanate Xi , Fharmfulhighly flammable, irritant(R32, S2, S50)R11, 36, 67, S2, 7,16, S24/25/26R:11:highly flammableR36: irritating to eyesR67: vapours may cause drowsiness and dizzinessS2: keep out of the reach of childrenS7: keep container tightly closedS16: keep away from sources of ignition - No smokingS22: do not breath dustS24: avoid contact with skinS25: avoid contact with eyesS26: in case of contact with eyes, rinse immediately with plenty of water and seek medical adviceS50do not mix with strong acids or lyses, nonferrous heavy metals and their salts.Emergency medical information in english and german language can be obtained 24 hours a dayfrom:Poison Information Center Freiburg, Germany: Tel.: ++49 761-192406Invisorb ® Spin Cell Mini Kit 032010
Product characteristic of the Invisorb ® Spin Cell KitStarting materialYieldTime forpreparationRatio- max. 5 x 10 7 cells(2 ml of overnight culture )- cell cultures(max. 2 x 10 6 cells),- swabs, serum or plasmaup to 10 Ag of genomicDNA depends onamount and type ofstarting materialabout 20 - 30 min A 260 : A 2801.7 – 2.0The Invisorb ® Spin Cell Mini Kit has been designed for fast and easy purification of highmolecular-weightgenomic and mitochondrial DNA from cell culture, swabs, serum andplasma.After cell wall lysis the starting material will be directly mixed with an optimized Lysis BufferD including reagents inactivating intracellular DNases and also DNases found elsewhere inthe environment. DNA is on the surface of a filter as contaminants pass through. Remainingcontaminants and enzyme inhibitors are removed in an efficient wash steps and DNA is theneluted in water or buffer, ready for use.The Invisorb ® Spin Cell Mini Kit avoids the application of toxic cancerogeneous organicsolvents as well as ethanol precipitation. The convenient, purification procedure removescontaminants and enzyme inhibitors such as proteins and divalent cations, and purified DNAis ready for immediate use in sensitive downstream applications like, or for archiving.LLLLLPCR applicationsdigestion with restriction enzymesSouthern HybridizationRFLP analysisHLA typing (DQA; DQB).Principle and procedureThe Invisorb ® Spin Cell Mini Kit procedure comprises following steps:1. lysis of sample material2. binding the genomic DNA on a Spin Filter3. washing the Spin Filter and elimination of ethanol4. elution of genomic DNAThis manual contains 4 protocols, according to the different requirements of the startingmaterials.Sampling and storage of starting material:Cell cultures:The yield and quality of DNA may depend on factors such host strain, inoculation, antibiotic,and type of culture medium. The cells will be pelleted after cultivation. Best results areobtained with fresh material or material that has been immediately frozen and stored at –20°C or –80°C. Repeated freezing and thawing of stored samples should be avoided, sincethis leads to reduced DNA size.7Invisorb ® Spin Cell Mini Kit 032010
Page 1 and 2: Instruction for the Invisorb ® Spi
Page 3 and 4: Kit contents of the Invisorb ® Cel
Page 5: Intended useThe Invisorb ® Spin Ce
Page 9 and 10: Important notesImportant points bef
Page 11 and 12: Scheme of the Invisorb ® Spin Cell
Page 13 and 14: Protocol 2: DNA isolation from cell
Page 15 and 16: TroubleshootingProblem Cause Commen
Page 17 and 18: Ordering informationProduct Package
Page 19: KoreaPhilekorea Technology Inc.www.

Instruction for the Invisorb Spin Cell Mini Kit

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