Multiple Antigen Labeling - Vector Laboratories

12Breast Carcinoma – Triple label• Estrogen Receptor (m), VECTASTAIN ® Elite® ABC Kit, Vector ®NovaRED HRP substrate (red).• CD34 (m), VECTASTAIN ® ABC-AP Kit, Vector ® Blue AP substrate(blue).• Cytokeratin 8/18 (m), VECTASTAIN ® Elite® ABC Kit, DAB HRPsubstrate (brown).Tonsil- Triple label• Multi-Cytokeratin (m), VECTASTAIN ® Universal Elite® ABC Kit,DAB HRP substrate (brown).• CD3 (m), VECTASTAIN ® Universal Elite® ABC Kit, Vector ® VIPHRP substrate (purple).• CD20 (m), VECTASTAIN ® Universal Elite® ABC Kit, Vector ® SGHRP substrate (blue/gray).Tonsil – Double label• Cyclin A (m), VECTASTAIN ® Universal ABC-AP Kit, Vector ®Blue AP substrate (blue).• CD20 (m), VECTASTAIN ® Universal Elite® ABC Kit, Vector ®NovaRED HRP substrate (red).Tonsil – Double label• Cyclin A (m), VECTASTAIN ® Universal ABC-AP Kit, Vector ®Red AP substate (red).• CD3 (m), VECTASTAIN ® Universal Elite® ABC Kit, DAB HRPsubstrate (brown).Breast Carcinoma – Double label• Estrogen Receptor (m), ImmPRESS Universal Reagent,Vector ® NovaRED HRP substrate (red).• CD34 (m), VECTASTAIN ® Universal ABC-AP Kit, Vector ® BlueAP substrate (blue).Mouse, Newborn (eye) – Double label• GFAP (m), M.O.M. Peroxidase Kit, Vector ® NovaRED HRPsubstrate (red).• Synapsin (m), M.O.M. Peroxidase Kit, DAB+Ni HRP substrate(gray/black).Note contrast with endogenous pigment (brown) seen insurrounding tissues.Mouse, Newborn (tongue) – Double label• Synapsin (m), M.O.M. Peroxidase Kit, Vector ® NovaRED HRP substrate (red).• Desmin (m), M.O.M. Peroxidase Kit, DAB+Ni HRP substrate(gray/black).Breast Carcinoma – Double label• Ki67 (rm), ImmPRESS Universal Reagent, DAB HRP substrate(brown).• CD34 (m), ImmPRESS Universal Reagent, Vector ® VIP HRPsubstrate (purple).www.vectorlabs.com