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Integration in ChromatographyCharacteristics of PeaksGaussian PeakInflection Point1 st DerivativePeak Time of GP atRoot of 1 st Derivative2 nd DerivativeInflection Points of GP atMax/Min of 1 st DerivativeInflection Points of GP atRoots of 2 nd DerivativeWorkshop | Bucarest, 19 March 201310 • 32
Integration in ChromatographyCharacteristics of PeaksEMG PeaksT f(0.31/0.18=1.722)T f(0.70/0.32=2.188)Retention time t R(6.58’)Retention time t R(8.46’)Capacity factor k’ (t R-t 0)/t 0=3.387Capacity factor k’ (t R-t 0)/t 0=4.640Tailing factor at 50% heightT f=b/a (0.21/0.19=1.105)w 0.5=0.40’w 0.5=0.49’Elution time ofvoid t 0(1.50’)w 0.1=1.05’IUPAC at 10% heightT f=b/a (0.72/0.33=2.182)w 0.1=1.02’w=~1.699×w 0.5=0.68w=0.830 1 2 3 4 5 6 7 8 9Workshop | Bucarest, 19 March 201311 • 32
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