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Integration in ChromatographyCharacteristics of Peaks1 st Derivative (slope)0 1 2 3 4 5 6 7 8 9 12 nd Derivative0 1 2 3 4 5 6 7 8 9 1Workshop | Bucarest, 19 March 201312 • 32
Integration in ChromatographyRecommendations•Capacity factor k’ for analytes >2•Example:(6.58–1.50)/1.50=3.39 (8.46–1.50)/1.50=4.64 •Resolution between two adjacent peaks•R s = 2 × (t R2–t R1) / (w 1 + w 2 )Baseline width w not accessible; for a Gaussian [sic]peak w ~ 1.699 × w 0.5 holds.•Desirable >2•Example: 2×(8.46–6.58)/(0.68+0.83)=5.69 Workshop | Bucarest, 19 March 201313 • 32
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