Application of Electroporation in - Dermavista.com

IntroductionElectroporation :It is a mechanism of transdermic administration of active substances through low-frequency currentsand high voltage (faradic) currents capable of incrementing the cellular membrane's permeability,altering the phospholipidic two-layer structure and producing temporary and reversible pores.BIO STIM features microcurrents to favor the penetration of ionizable active principles, optimizingresults.This technology takes advantage of the cellular membrane's weakness and its capacity of recoveringafter the electrical impulse that produces the pores' opening.Mechanism of Entrance :Electroporation allows for the arrival of the product to the dermis (as mesotherapy).The dermis is the skin layer more vascularised, and as such, has the function of absorbing andredistributing medicines. Besides, its structure eases this spreading in a sustained and prolongedway.First, the mechanism of introduction is transepidermic and transfollicular. Once in the skin, itbecomes transcellular and intercellular, being the latter the most important. Finally, the productgoes into the intracellular space for a difference in the concentration gradient.In different studies, there has been described the passing of particles from 0,2 µm, 4 µm and even,45 µm.In other study conducted by TDES, it is established that the intercellular spaces provoked by theapplication of pulses adapt their diameter according to the size of the particles going through them(the diameter of the spaces between 2 cells being from 0,8 µm to 3,1 µm, and the diameter of theparticles of chinese ink being of 0,15 µm)This phenomenon is created with short pulses (1,2 msec) of high voltage (120 V).In the same study, there can be seen particles of chinese ink in the cornea surface, follicular pores,keratinocytes cytoplasm, in all the layers of the epidermis and the papillary (superficial) and reticular(deep) dermis, without the necessity of applying a keratolytic agent (urea, salicylic or lactic acid,among others).

PhotoageingIt is a multifactorial, dynamic and complex process, that combines intrinsic (genetic,nutritional or hormonal) and extrinsic (ultraviolet rays, smoking, heat, cold) factors .The resulting are cutaneous modifications such as dehydration, wrinkles and flabbiness.There is a classification proposed by Glogav in 1990 that considers ageing levels depending on thepresence of keratosis, wrinkles and dyschroa.Glogav ClassificationAgeing Actinic Keratosis Wrinkles DichromiesSlight (25 to 35) - Few MinimumModerate (35 to 45) + Dynamic BrownishAdvanced (45 to 60) ++ At rest Yellowish BrownishSevere (60 to 75) +++ Gravitational TelangiectasiasTRANSDERMIC ADMINISTRATION VIA ELECTROPORATION IN THE TREATMENT OFCUTANEOUS AGEINGThe case study was performed with a total of 6 patients.Inclusion Criteria:-Women-Age between 40 and 60 years-Cutaneous Flabbiness-Cutaneous Ageing.Exclusion Criteria:-Under cosmetologic treatment.-Facial Aesthetic Surgery in the last 12 months.-Active Skin Lesions.-Pacemakers.-Epilepsy.-TAH (Total Abdominal Hysterectomy).-Pregnancy.-Cardiopathies.

Diagnoses :-Clinical Evaluation, preparation of Clinical Record.-Hydration and Elasticity with Multidermascope MDS-800.-Photographic Record.-Ecographic Record.General Objectives:- Indirectly prove the effect of substances introduced into the organism through Electroporation, forthe treatment of photoageing and cutaneous flabbiness.- Produce subjective changes (reducing wrinkles, providing more firmness to the skin, improve thequality and the skin appearance)Specific Objectives:●●Improve the skin appearance.Diminish the evidences of age and cutaneous flabbiness.Intervention :It was used the Biostim equipment, made by Starbene, Europa Line, serial nº PR 0001.It was performed the sensitivity test, applying a drop of the product on the skin of the inner forearm,24 hs before the beginning of the treatment. It was applied 1,5 cc per area of 10 per 15 cm (total 3cc per session) of the solution of active substances (DMAE 1% + hyaluronic acid 0,1% + Colagen 5%+ ELECTROPORATION SPECIAL VEHICLE), of lipolytic and anticellulitic action, through electroporation,previously applying an antiseptic substance.It was indicated a solar protector and extreme prevention when exposed to the sun.Duration of the Session:7 to 12 minutes.Duration of the treatment:10 sessions during 5 weeks.Frequency of Application:The frequency of application was of 2 sessions per week with an interval of 2 or 3 days in between.Statistical AnalysisMultidermascope MDS-800

Multidermascope MDS-800 is the tool used in the case study for the skin diagnoses. It featuresvarious measuring probes:Hydration - CorneometerElasticity - CutometerCapacitancePressure: approx. 0,16 N/49 mm²Frequency: 0,9 – 1,2 Mhz.Suction PrincipleOpening: 2 mm diámetroTime of Measurement: 6 seconds.Results :All the patients experienced subjective and objective improvements.It was evident a noticeable attenuation of the expression grooves. The skin presented morefirmness at palpation.Elasticity, hydration, the skin quality and the general appearance of the face improved in all thecases.There were registered changes from the fifth session.Measuring Record with Skin Analyser (Multidermascope MDS-800)HydrationbeforeHidrationafterElasticitybeforeElasticityafterp 1 80 99 49 53p 2 83 87 54 67p 3 85 90 71 78p 4 99 99 41 63p 5 86 95 59 76p 6 82 89 48 65Modification AveragesHydration : 7,33Elasticity : 13,33

Patient 3Patient 4

Adverse Reactions:Painful sensation due to the excitation of nerves and proximal muscles, itch, tingling, erythema anddiminishing of the electrical resistance of the skin under treatment (all the patients endured less thepassing of the current along the treatment), this reactions are slight, reversible and temporary.At a histological level and after applying Electroporation for 12 minutes, there have not beenregistered tissular alterations, not even the slightest ones such as tumefaction or cytoplasmatic ororganelle vacuolization nor irregularity of the nuclear and cellular membranes.Discussion:The main inconvenience presented by the topical administration of active principles is the barrierposed by the skin to any external substance. This new and revolutionary technique in Aestheticsmultiplies the effects and optimizes the results of topical applications and of ionization of cosmetics,since it allows for the incorporation to deep skin layers of active principles that cannot penetratethe corneal surface by other means.It is possible then, to use specific drugs to treat flabbiness or cutaneous ageing.Conclusion:The measurement carried out with the skin analyser MDS-800 showed in 100 % of the cases anoticeable improvement regarding skin hydration and moisturizing.All the patients noticed positive changes in the skin quality, stating that “they look younger”.Bio Stim was efficient in the treatment of aged skins, attenuating wrinkles and expression groovesand incrementing hydration and elasticity.It can be mentioned that in all the cases there was registered an improvement in the skin quality,changing the general appearance of the face, which was evident after the fifth session and morenoticeable at the end of the treatment.Hydration and elasticity incremented values, as well as photographic records and the patient's oralaccounts prove the effectiveness of Bio Stim's Electroporation to introduce specific substances intothe organism, optimizing their action.Scientific Direction: Sibela González, Medical Specialist in Dermatology.Member of the Dermatology Department of the Hospital Nacional de Clínicas, Córdoba, Argentina.Colaborators: María Gabriela Ruiz, Physiotherapy and Kinesiology Licenciate. Andrés S. Valinotti,Physiotherapy and Kinesiology Licenciate. Damián A. Torletti, Physiotherapy and Kinesiology Licenciate.Jimena Quagliotti, Apparatology Technician.