Improved Print and QC Methods for Oligonucleotide Arrays
Improved Print and QC Methods for Oligonucleotide Arrays
Improved Print and QC Methods for Oligonucleotide Arrays
Create successful ePaper yourself
Turn your PDF publications into a flip-book with our unique Google optimized e-Paper software.
IDT NonamerCy3 -JAR vs Cy5 -referenceFigure 6: IDT’s <strong>QC</strong> Product Results vs. Experimental Results on High-Density Array.Left image is from a 1 hour <strong>QC</strong> hybridization using the IDT Cy3-nonamer. Right image is thecorresponding replicate sub-array from an overnight experimental hybridization. White arrowsindicate examples where the absence of experimental hyb signal is due to absence of mRNA target <strong>and</strong>not absence of spotted PCR probe. <strong>QC</strong> hybridization image is 5µm resolution Cy3 scan using aScanArray® 5000 (Perkin Elmer) at 73/73 laser (power/gain) settings. (Pseudo-color scale:black
Change language