VOLUME 1, No.1 VIRAL EPlZOOTlCS IN SHRIMP - Central Institute ...

VOLUME 1, No.1 January - March, 1998.CENTRAL INSTITUTE OF BRACKISHWATER AQUACULTURE, MADRASVIRAL EPlZOOTlCS IN SHRIMP'Ihls 1s a general artlclc on \In1 dlscaocs of shnmps \rhlcl~ havc affcctcd theaquaculture lndusrn oflndladurlngthe 1st t\ro! cars Rcscarch on thc v~nl dlscasesof aquatic orgmsms under culturc arc rcccnl aid havc ken largely concerned w~ththc dc\clopment ofdlagnost~c tcclln~q~rcs More ~ccntl!. work on DNA probes forrap~d dlagnos~s has rccencd attcntlon As of no\\. rcllahlc control ncasurcs agalnstvlral dlscases of shr~mp arc not a\allablc Pre\cnt~on through health! and h!geln~crnmagcrncnt prdcttccs can help rcducc losses 1111s artlclc alms at ~nfonnlng shnmpfanncrsllndustn for grunlng an undcrstu1dll)g Into tllc problcn)The Indian shrimp culrurc industr! is 111 .I alni of this article is to educate and ~ncreasc thestate of depression within about tlucc years of uniicrst~1ding of thc farlncrs 111 ilcaling with ther~d~ng the crest of a hoorrl \va\.c. 011 rhc OII~ major v~ral disc.~\cs. It would also deal withIINI~, ~t collfro~ltc~i .1IIcgc~i ~(~lo-eco~loniic .IIILI kno\\.li prc\.c~~ri\,c nic.t\ures which u-ill hc ofhelpcn\ironmcntal ~icprad.lt~on i5suc.q In ccrtaln arc;L\ to the fm11~rS.of coneenmarlon of shrinip farnis aid, 011 tllcWhat are viruses and how do theyother, shrimp dlscnsr cp~/.)orics have c.1uscd cause disease 7significant prcduction and cconolnic los\c\. 1111994-95, loss of shrimp produztiol~ due to \'iru.\cs arc particle\ a~hcih can hc seen onlydisciuc\ a x cstimatcd bcnvccn 10,000 and under high pourer clccrron tnocroscope. Among12,000 ronna. The mend h.t\ continued througlirllo~c knou~n to effect shrinips, the stnallcst one1W5-96. These prohlcms tvcrc nor ~lricxpcitcdrnc.t\urcs ahout 2211111 (one nrn is one millionthas ivc had nu~ncro~~s example\ of \imilarof a nlillimcrcr) and the Iarpcsr arc haculovirutcs,mc.z\uring 75- 3Ohn. \:inlsc\ arc made up ofslruarlolls u~ 'L~~van, the I'hilipp~nc\\, Thailandnuclcic acid, cithcr the dcoxyrihor~uclcic acid.ud China, ns also in F,cuador. The alleged stxial(DNA) or rihonuclcic acid (KNA), which isand cn\~ironmental is~uc5 could hc rcsol\,cd hyolcloscd by a protein coat. The protcin coat ispromonng people's parric~pato~ progr,unmc\host-spccific and aids in the prtxcss of ulfcction.and srrict implementation of the existingThc virus attachc~ to specific site on the host cell~widelina legal frmc\vork of the WLIIIU!:and injccn it\ nuclcic acid into the ccll.The orhcr mapr ksue of v~ral disuw cpi~~u)ricsin shrimp fu~ns may be conrained \\,it11 a dccp Upon entry of viral nuclcic acid into theundcrsm~ding of the proniblems in\.ol\*cd. The ccll, the 1)N,4 of the host cell is broken down1

-fmportant viral diseases of penaeid shrimps known in the worldnoStS~rnd- ---- --on the toP& Geog_rapFaya _--1. 8y8iemIcedodamral Pmonodon All slzes affected. 100% Chlna, lnd~a,and tnrmderml P indicus morlalny wlthln 3-5 days Japan, Thailandbrarlow~s (SEMBV) P lsponiarsP chrnsnsrsP merguiensisP vannamr2. Monodon-type P monodonbawlovlnts (MBV) P indrcusP esculentusP semrsulcatusP mrgurensrsP penrcellatusP kerattturusP plebelusLow level mortalil~es,onen due to secondarybaderlal ~nfect~onsTarwan, Indonesia,Australla. lnd~a3. Yellow head baculov~rus P monodon Above 5g affected Tha~land. Tatwan100% mortality wrth~n3-7 days4. lnfectlous hypodenal P vannamr High mortallty rate Hawal~. Panama,hematopoletic necrosis P styirmstns Costa R~ca,virus (IHHNV) P monodon Ecuador. Tah~t~P semrsuicatusSingapore, Israel,Taman, France, Peru5. Baculovirus penaei (BP) P duorarum Acule eplzootlcs Hawali, Flor~da, Texas,P eztecus In larval stages Mlssls~pp~P vennemer 10036 mortality ~nP margrnatus24-48 h6. Baculoviral mid gul P jeponrcus Ser~ous ep~zootlc ~n Japangland necrosis (BMN)hatcher~es upto 98%viruswrnulatlve mortality7. Hepatopancreatic P mequrensrs Poor gmwlh rate. Southeast Asla, Ch~na,parvo-like virus (HPV) P semrsuicatus 50-100% mriallty ~n Australla Pen~an GulfP chmensrs4-8 weeks of onsetP esculentus8 Lymphoid organ parvo- P monodon Not known Australlalike wrus (LOPV) P mergurensrsP escukntusO Reo-like wrus (REO) P monodon RE0 lnfectlons onen France, Hawall. ChinaP japonrcusoccur along w11h otherP vennamerpathogensP chrnensrs10. Taura Syndrome P vannamr Heavy mortality dunng EcuadorVlrus vSV)15-40 days cuiture. -- -. - penod -- _._ _---~ypr of WNS S No 1.2,3,5 and 6 - Bsculavlrus S No 4 7 and 8 - Pa~c-llke vlrus S No 9 - Reo vlrus S No ID - Nodav~rus

inn) small subunits and chesc are utiliz~d forspthesk of che \iral nucleic acid. Thus, che \irdnucleic acid is repliatd many fines. \lira1 DNAalso directs the cell (through nicsscngr KNA)to s!nthcsu*. protcuis that arc required for itscoat. Thus, the complcrc \iruscs are pnxluccdusing nc\\.ly formed topic.\ of tirat nuclcic nc~d\viU hc. rclc.z\cd outside up)n rupture of rhc ccll\vhicIi cxi infect frcsll Iic~lrhy cells to continuethe cycle. \'iru\cl\ hy d~ni~~cl\.c\ cxinor po\v ornlulnyly xq hacrcr~n or othcr lijing orgmilsnls rlo.Ilpon cntn, into the hosr ccll, rhc \.iruscshring ahout sc\,crnl ch.uigcs i\.irlii~i the ccll.\'arious al.rnorm.ll grnnulcs call he oliscr\fcd\virli~n vlms ~nfcctcd cells. Tlic\c inclusions mayhc. agpcgntc of \~n~\c\ or ~norlifiril ~,c.llul.~rco~nponcnt~. The ty[x'\ of thcsc ~nclusion\ ,111rltlic~r IOditlOll \\.1tll111 the CCIItoeach \.irurcs.RTC ~llar.iitc~-~st~i'The \.iru\c\ thus ilctroy the cells ofdifirc~ir tissuu. ((iiscqi~cnrl!; the f~~ncrion\ ofthe organs of \hrinil> nrc .ilfccrcd Icad~ng ro~i1sc.1~~ :LI~LI 17s q\~nipto~n\. The \prcn~l of' \,trus~nfkt~on is so f.nr rhar 111 thc cnc of ycllo\\, lic.lilor the \\.li~rc 5por. vlru.\ ~nfcct~ons (;il,out 3-4d~y~i.How viral diseases spread in theshrimps?injuries on the M y . Once inside the body cavityof shrimp, the viruses may hc carricd to thcirtarget organs through haclniolymph.Recent viral disease outbreaks inIndian Shrimp farmThc epi./~x)ric..\ affccring the shrimp f,mnsin niost of rli~ 11i.iriri1iic states of OIU cou11trydur~ng the p.~\t n180 yc.lrs ha\^ ken caused byvin~sm, ~nor.ihly, thc ycllo\v head-like virus N I ~tlic systcnilc cctodcrm.il and ~i~csoderrnalliaculo\~iri~\ .\(u.liitc spot discz~).Yellow head(YHD)-like diseaseTlic first m.ijor cpixootic of culturedsliri~iiliw~i 1nd1.1 \\,.L\ rccordcrl rluring Julj, roSclitcnil~r 1994 111 rlic slirinili far~ns .ilong thcK.in~l.llcru creek arc.1 111 Ncllorc riisrrict ofAnii1ir.1 1'r.iJcsIi. 'l'lic cliin)otic tiger shrimp,I1cn.lcu\ niontxlori \fpn.ilfccrcd by the disexqe.Al.i\r nio-tal~y of shrinip was artrihutcd to1~r1111ary \'irnl infection hllowcrl by sccon~iqIi~crcr1~1 infection. Sliri~iips \irere lost ~ ithi~i 3.5d.iy\ of ~nirial niort.lliry It was not possible fortl~r f.irnicrs to Jctcct rlic onsct of rI~\easc sincetlic affected \lirinilis d~d not cxhihit any\ynil>tonis. AItIiougli I>.lttcrn of disc;~+c ;11idriiortnliry u,crc \inlilar to rlic yclloi\~ licad diseaserclx)rtcd frorii 'IIi.~il.uid, the Kandalcrn cl~sea..cniarkcdly dilfcrcd froni Y HI) in li~stoparhologySninll crust.iccans and othcr aquaticorg.~nisnis Inn!. c.uy shrimp parhogc~lic \.ir~~\c\nnri ~f tlirsc enter i~~lturc ponds can i.lu\c1111i.it101ls m h~~ltli!. slir~mp. Hc.dthy sIirinil>sWhite spot diseasebllrhirc spot disci~sc, scientifically hiown asS!S~CIIIIC r~toclcr~ii.iI anrl nicsoder~nalliaculovirus (SthlI3\') h.~\ Ixcn the major causeInn!. hc .affected h! fccd~ng on 111f~it~rl dead of Iargc \talc niortalitio of ct~lrurcd shrimps inaqunrlc an~mals or cannih~l~snl o~infcctcdnlorihund ,urimals. I'iral d~seztm in groiv-ourpolid Inn!, also lie rr.uis~nittcd through h~rds,\vllicli may plik up infcctcd and dead slirirnptand drop them in uriinfcetcd ponds. Infccticjnmay also take place when the Iv~mpt injcstpathogenic \.iru\cs prescnt in the contaminatedrearing i\.atcr. The iirusa may gin cntr!. intothe shrimps rhrough abrasions, wounds orIndia. The discz\c i4.s firsr reported stx)~i dterthe onset of Norrhc,zct Inonsoon in Novcmber1904 from some shrimp farnis in Nellorc area.S~ncc tlicn, rliis L~ISCA\C lia ra\lagcd shrimp farmsin al~nosr all the niarit~me statcs of our countryand 11.u bee11 rcsponsiblc for hruigi~ig dewshrimp prtduction from the culrurc sector.Unlike the YHI), wlicih affects only I? mo~i(*n,this di5c.u~ affects both tiger and white shrimps

In addition to rhe above, the yw,l!nlersc Irlcasurcs are dc\.cloped hascd on scientificchain reaction (I'CK) is a very recent enay undcrsnndi~rg of rilc parhogens and rhc shrimpanlong the daglostic tccluiiqucs. This rect is immune sysrern, a\.oidencc appears rn he the o~llyhighly scns~ti\~r N I derecn ~ \.cn Ion ~]u;u~titic\ 111~x1s of cornhatring \iral diseascv in rhc slui~iipof DSA of the ~l~icro-orgulism and is uschl in f.m11s.dctecring infecrio11s ul very early stagr. This resthas hccn Jcvcloycd for dcrccrion of hlR\'GENERAL GUIDELINESi~~fcctions in shrimps hut not yet co111mcrcialiseII. 1. Dry rhc ix)~lds and plough. Fill ahout 10 -15 em of \vatcr and \\air for 3 days n) allowAre 'cures' available against viraldiseases?c~lc!.trc~l orgalisms to hatch. I)isi~lfcct bychlor~nation (N 10 - 15 ppnl of clllori~lcAttempt5 ro control \iral ~iiwasc 111 poultry us~ng .~pllropriatc qql~a~irirics of comllicranimals,llum.uls and pln~lts hy cllcnlothcr.~liy ci.llly .~\~.~il.~Iilc tilc;~clii~~g po\v~icr (calciu~i~lia\.c nch~cvcd limircd succc\\. Appro.lch to hy~xxhlor~rc). Allo\v for rhrcc days hcforccontrol rhc viral disc'iscs In rhcc orca~lis~ns .~ppl~cario~l of fcrril~scrs an11 manurcs.through propllylncr~i \,accincs Iln\ y~cl~lctlI'ro\~l~lc nvo rcscr\~oir pon~ls in the farm.yrorn~s~ngrcsults agn~nsr i~nyorrant ~II\~;L\cs \IICII111 tllc first po~ld, .llloiv scdin~c~~tarion ofa\ s~nall pox, pdio and rnhics in hu~n.~~ls, f(r)rS~S~KIILI~LI matter. l)~sinfcct hy applyingN I 11loutI1 ~ discasc, rahics, rin~lcr j~st, calllnc10 - 20 pp111 cl~lorinc anif mix rhc ponddistr~nlpcr ctc. In .umnl.lls, and fo\\,l pox, ncn\\.nrcr usi~lg .\LICL~I~~L* nu~llhcr of ~i,lddIcchtlc C!~SC.ISC, cti.. ill l~ultr!: Th~sllcits\ Ih.1\\\,l~eeI aerators. ,Allo\\, rhc chlorinc to acthccn ~nainl!. ntrrihutc~l to rllc cxrcns~\~c rc\cc is yc.t tc~ !,~cl~i sccon~l rc.\cr\,olr 1w)n~li)r allout a dn!. IKproph!.l.~it~iand corlrrol n,c.z\urc ag.llllsr ~ I I C fi)t.c using fix c~~lturc acti\z~ry.hccn rake11 up in .sc\rr.~l Inhorntoric\ around thr\vorlil \incc onl!. ahout a ~Ie~acic go t11e~ 4sn~~i~c\ h~\,c 1~c.n fi~~ulci to hc LL\C~III ill L~I.I~IIO\ISof \.ir.zl disc~$c$ .ul~t illaractcr~\~ng son~c \,lruc$.The in1t1.11 \vorh on rlic dcfcncc sysrcnl Il.l\.cindicated that i~~ilkc rhc higher an~nlal

chi disc d+ of 40 - 60 cm) in the pond R & D HIGHLIGHTS DURINGduring the culture period.1995 - 967. Make routine obs~warions on the healrh ofshrimps, K~~~ vigil on sw,mnling h. Development of broodstock of tigershrimphaviour of shrimps. Examn~nc shr~mps forany signs and symproms that xc externall\Ti) reduce the dependence on \v~ld gra\~dvisible. Take help from expcrts on \Ilrllnpfcm.llc\ of t~gcr \hrlmp tll i~u~riodor~ ) fi~r h.~rcllenhealrh whenever abnormal co~ld~t~onc arco~xr.ltro~i\. 000 yond-h~o\w \pcclnrcn\ \\.rth ;mnoticcd.a\,cr.lcc \I/. of33 g ivcrc rc.lrcil 011 n ~llcr of( :I13.4~>cllctl\c~l fccil nnil ioolccl ilnni mc,lt In .I 0.0758. Observe a gtxxi fcr~l~ng prognlrl~nc u\tng11.1 pon~l ,lt Alurruk.11l~1 i-lrl~l (21lrrc 01' thrhalalrccd feeds. Warclr fkcdrng hcha\~~o~~rIri\t~r~~tc, Tlic \Iirr~np\ Il,l\c rcc~cl~c~l ,111.i\,c~-~g~,of shrllnph rckqllclrl,,, I~luL.tll~lrlOll\ rllc$ 1 of ~ 77 g Sor rli,~lr< ,111~193 g h)r l21ii.llc\ 111rates of f'ec~l collsunil>rlorr I\ ,111 I I ~ L ~ I L . I ~ I ~ I ~of had hc.1lth of \hrt~llj>.0-rllonrh rc.lr111g .ul~i rhc! i\.crc n.ui\l'crrc~i toIah~r'lror~ r.ulI,\ SOI 111~1u~'cii lri~lnir.1rron tr~~l\.I-c1ii.11~ \Ilr11111>\~it~lcctc~I to LII~II.I~C~.II c\.c-\t.iIJ\9. A\,oid \v.lrcr cxch,lrlgc \\ Ilcllc\,cr ,111 cpr/o,~l~I,lrr~~rr l i ~ \LICL~S\~LIII! \ ~ I~~~~LI~L.LI11127 LIJ\ \ ~ I I ~ Ioric is notlccil In rllc ~lc~~ghho~~r~irg Llrnl\.g:l\.c \,~nhli, \p.l\\,11111g\.10. I>csnoy discasc~l/ilc.l~l slirlnll>\ clrhcr h!. ~~~~d sto k h 1 ding fa i 1 it biuningor hy buryl~g\\.~th I~nlc.i\v.r! fiolll seabass and mulletforrhc farina.C'.~prr\c 13roo~i\tol.I, of \i~L~L~~\\ (I.~it~,t11. I'rov~dc ,I pon~l to allo\v trc.lnilcnt o1'f:lrnl ~.~~/~i~nf>rj an~l grey 11iulI~,t (hfzytl 1~1~111tilu1~emuc~lrh hy scrtlcmcnr .ul~1 dl\~nlk~tion rcnrcd 111 co~rfrnccl c.~rthcrn pon~i~ \\.ereprior to d~schnrgc into ndtur.il \r,dtcr I%)L~- tr.unsfcrrcd to 100 ton c.ip.lcln KC:(' t.unks \vrtllics. floiv- through \c.lrw.tter \!\tern. For the first12. Ik, nor rclcnsc d1se~~se~i/~1e~rd \11r11111>\ .UIL~\\.arcr from .zffccrcd pond.+ Into 1l.1nrralwater hodics. Flol~l 'r~id d~sillfcit s~li.li\ttarer \r.irh chlorinc hcfi~rc d~sch.ugc.13. Do nor use rhc s~llnc cyulpnlclrr in .dl thepnds. I>o not i\..~\h rlrc cLlLupment 111 rlvilller water canxl. Wash rhc farm .implcrnelltsaftcr every ilsc with detergent .ulddry.This article was prcp.ucd hy rlrc follo\v~ngscientists of thc Insrin~rc, Shri S. 1: Ala\,uld~,Dr. K. K. \'ijapr, I>r. K. \! Rnjc~rdr.ur, Shr~ Al.S. Shashi Sckar, Dr. S. 5. hlishrd .uid Dr. T ('.Sadago.created hy (:IHr\ at Alurnlkacl~~ for r;nrlnp fishhrotxlstc~t un~lcr cajltl\,c corlii~t~oll.Shrimp disease investigationhl~crob~olog~i.rl arid l~~\toy.~tliolog~c.~lsnldrcs conducrcd on rlie dl\cascd tlgcr .ui~l wh~wslu~nip~ohtn~nc~i from the shr~~np farms fromOrissa, Andhra I'radcsh, Tx~r~l Nadu xrd Kcralah.1~ sho\vn th.1r the ivhltc spot d~scsr, alsoknoun s SEhlH\' iS!.stcrn~i F.crcdcr~nal xldhlc~txlernral 13aculovlrus) dlscasc \~.LT the ma~orwuse for I~gc-si~l~ ~ti~rthna. Tlic hIon(donR.?culo\~ru\ (hlH\') rh.\c.ue \\.a also fotu~d ul thesalrpla ~fslmrrr~n fro111 dlc LXIIWC pnd~ x~d dlcImac fn)~il die h.~riher~a.

ClBA shrimp feed testingThe shr~rnp fcai dc\~clopcd h\ thc I~i\r~n~rc\\,a\ tcrtc~l In n tar~ncr'\ field at Ongolc, A1idilr.1l'rndc5h The tlgcr \hr~n~p \cccl\ \tc~Lctl nt ,i r.xcof 67.5()0:'h,l 111 .I 0.4 h,l lw~nd ucrc fc~l \\~tll(:IIIA fccd al~d the\ hnvc attalncd nl1 a\2cr.igc 5111.of 22 g 111 113 daysDr. 1:\'1< I1illa!: Eornicr EAO Aq~~aculturc( i)n\ultnn r, 1)r. R. Nntaralul, Krd I1rofc5sor,VISITORS(kntrc of Ad\fanccd Stud! 111 Mnr~nc I31olog);Paranglpcttn~ and 1)r. H I!(' Shctr); Rtd.I'rofcs~or, El\hcr~c\ (i)llcgc, Maigalorc, 23-24MadrasEcbruar!.' 961)r. R.5 I'nrtxla, D~rcctor C;cricral, I('AI

Confaetice on lnformat~a for sustmableagriculture dtvelopmmt at New Delht, 23-25May 95.National Work5hop on poultr); fisher~c.\and food proccesslng In Ind~a at NAARM,Hyderahad, 4-5 JulyFirst meeting of the Tcchntul ~J .11i~i1)1 \ \ h11\l11 I, \LIL,I~II\~\\u~\c\cdrhc \lir~nlpf.urn\ .lHc~rl~l \\ lrh ~i~\~nsc prot>lcnl\ 111 Ilnl,~sorc.IIILI l3li6ltIr.lL l)l\tr~~rs of Or~\sn, 23 2Y April1)r A1 hrt

Fisheries and Food Proccessing in India,NAARM, Hyderahad, 4-5 July.training at SEMI>E

Principal Scientist, CIFA and scientists from Three officials from the 1)ept. of Fisherla,CLBA haw participated in the meeting. (;on. of Tamil Nadu were uained in shrunpdc~ea~c diapostic~ at Headquarters, 26 June - 1The meeting of che Institute's Joint9s,Council was held at Madras, 16 December 95 and15 March 96. Shri. S.V. Alavandi, Scientist (SS,The Second Meeting of the Researchunderwent a 40-days training course in microhiatfernlentation at Uni\crsi~ of C;l~sgcn\; LhtcdAdvisory Cmmmitteeof the Institute nr~\held on23-24 February 96 under the Chairmanship ofDr. T,VR. I'illay, former FA0 AquacultureConsultant. Dr. P.V Dchadrai, Ileputy IlircctorGeneral (Fy), ICAR, Dr. K. Nanrajan, Rtd.Director, (kntrc of Advanced Study in MarincBiology, Pum.unalai Ulii\~crsin, md l)r. H.l?(:.Shetty, Rtd. I)irccror, E'~shcrics (:ollcgc,Mangalore, the RA(: manhcrs attended themeeting and visited the Muttukdclu Ficlii (intrcand had disct~\sion \vitli the scicnt~srs of rhcInstiturc.TRAININGUndcr thc World I%ank-x\sistcd I'rojc

Shri. S. Rajesharar Singh as '11-3 at Shri. R. Bdakumaran as SS Grade I atMadras, 1 Decenther.Madras, 20 Srpwn~ber.Shri. J. Jocsph Sahaya Rajm as T-11-3 at Shri. R. Kumaresan as SS Grade I atMaha, 8 Dcccmher. Madras, 27 March, 96.Shri. Marella Kni &,z\ T-11-3 at hlacira, 11Ikcemher.Shri. C. An.ulthanarapnnal xs T-1 at MaLira9,6 Ohher.Sliss. C:hhanda Mazu11ld.lr ns T-1 atIiakdtvip, 28 No\rmnhcr.Shri. Ii. Jagm Mohm KJJ us T.1 at hl;ldrx\,15 Fchruary 96.Slui. hl. (:hinlnakupp.un LY T-1 at hlntirx\,26 FchruaryAdministrative personnelPromotionsShri. A.H. Molndnl, Senior Clerk nsk~sisnnt at Iiakd\\ip, 6th Jmlc 95.Shri. 1:N. Ilajasdaran Nair, Junior (:lcrkns Scnior (Jerk ,lr Nnmkkal, 25 Ma!!Slnri. I1K. 1cccmhcr, 95 Assistant ) at Iiskdu-IF, 18 No~rnihcr.hliss. I

No.8.Merhodology for seed production(1995).Backyard hatchery techlology for thewhite shrimp Pcnncus indicus (1995).No. 9. Marine prorein resource a\,ailahility inIndia ac raw material for prawn feeds(1995).CIBA Special Publication1995 for his Docroral Research work on No. 1. Scnr~nar on status and prospects of"Molecular Characterisation of F0~1 pox Virus:hracktsh\\,ater aquaculrure in OrissaAnalysk of protein and geno~nic profile of (1988 ).vaccine strain and field isolarrq", carried our at N(), 2, l,f!orksllop hrackishusatcr finfisllIndian Veterinary Keqearch Institute, Izaniagx.I~rccd~ng nn~l ccd production ( 1989).ClBA BulletinPUBLICATIONS(Yeer of puMlcabon In parenthes~s)No. 1. An overview of brackish\varcr pcn.~crdshrimp and finfish culnlrc rc~cnrclr InIndia in 1980 (1987).No. 2. Prawn farriling - C:.i~rdidatc spciics(1992).No. 3. Shrimp discam, their prc\renrlolr andcontrol (1995).No.4. Technology for Anniru? cyst andbio~nnss prtxlucrion (1995).No. 5. Micropuriculatc Ski for postl.~rv;i~. ofshrinrp 1'crrncu.i ~rrd~crr, ( 1995 j.No.6. I)cvclop~ncnt of hrooclsro~k .~n~lmaturnrion of tiger pr,i\vn 1'r.rmrrc~tiroru~dotr in cal.rti\in ( 1995).No.7.Biology, fishcry, culrurc .~nd seedproducrion of pearlspot 6t?r,i,lrc~srrratrtkt.rb (Rloch). l'art I : R~ology.fishcry and culture. l'.irr 11 :No. 3. Training m;ui~~al on hiorcclinologynpp~~achc\\ In prdun ard fish nutririo~rand Sccd tcclinology ( 1995 ).Tra~nrrrg courGc trratcr~d - Scnii-~nrcris~\~c shrinrpil~lrurc tcih~lolob?, 11994).C~BA ~~~~~~i~~No. 1series (1996)Shrrniy culn~rc - I'onJ prcpxarionN0.3 Sllrrnrp cttlrurc - Fccds and feednr.ui.lgclnclit.N0.4 Shr~rulr iulnrrc - I>t\c.tsc and hc.~lrhni,ur.lgcnicnr.No.6No. 71.1rnc and tts applri~ttons inhrackishuarer aquaculrurc.Tr.~ining progr;uirmG In (:IBA.Publishtd b! Dr K Alngm\\am~. Dlrcctor. CIBA. Mndr.ls Edlrcd b! Shn M Kntluncl, Dr K Gop~w~lwn.Shri N Kdanlan~ and Dr (Mrs I Mliw\\nr Sul~a~w. Selllor Scicnt~sts. CIBA. Madca