full issue - Association of Biotechnology and Pharmacy

Current Trends in Biotechnology and PharmacyVol. 5 (1) 998-1003 January 2011. ISSN 0973-8916 (Print), 2230-7303 (Online)Optimal Production Conditions, Cultural and PhysiologicalCharacterization of an Active-inhibitory Compound(s) ProducingStreptomyces Bb36 Isolate against MultiresistantBacterial pathogensIsmail Saadoun 1 * and Mohammad Alawawdeh 21 Department of Applied Biology, University of Sharjah, Sharjah, UAE, 2 Department of Applied Biological Sciences,Jordan University of Science and Technology, Irbid-22110, Jordan* For correspondence - isaadoun@sharjah.ac.ae998AbstractA Streptomyces Bb 36isolate was found toinhibit multi-drug resistant bacterial pathogens.When the activity of Bb 36Streptomyces isolatewas compared to 23 standard antibiotics, datarevealed that this isolate may not produce any ofthese antibiotics except cefotaxime andcefuroxime sodium. Bb 36isolate began to produceinhibitory bioactive compounds againstEscherichia coli and Staphylococcus aureusafter 5 days and optimally in yeast-extract maltextract(YEME) broth at 28 °C with inhibitionzone diameter = 8 mm. Ethanol extraction andevaporation of the culture filtrate revealed activityagainst E. coli and S. aureus at MIC of 10 and 5mg ml -1 of the crude residues of evaporatedethanol extract with 8 and 11 mm inhibition zonediameter, respectively.Key words: Bioactive, Compound, Conditions,Extraction, StreptomycesIntroductionActinomycetes and particularly membersof the genus Streptomyces are the most importantindustrial microorganisms because of theircapacity to produce numerous secondarymetabolites particularly antibiotics (5, 10) that mayultimately find application as anti–infective, anti–cancer agents or other pharmaceutically usefulcompounds (1). However, in the past 20 yearsand due to the increase in the resistance ofnumerous pathogens to common drugs, thefrequency of pathogens that cause invasivediseases has increased dramatically (2). For thisreason, intensive screening programs forantibiotics are still running in different countriesof the world and mainly from genus Streptomyceswhich provide a rich source of antibiotics (3, 12).The traditional approach to/for isolationhas been the use of terrestrial soils, which providea rich source of these microorganisms (4).However, screening programs are still beinginitiated in various countries for isolation of suchantibiotic-producing Streptomyces strains mainlyfrom soil samples with new strategies thatcombine broad spectrum of activity against testpathogenic bacteria or fungi that are resistant toseveral known antibiotics.In the course of our screening programfor new antibiotics, Streptomyces Bb 36strain, wasisolated and characterized. The bioactivecompound(s) produced by this strain showedbroad-spectrum activity against severalpathogenic bacteria that are resistant to differentantibiotics. Therefore, the purpose of this studywas to examine the optimal conditions forbioactive compounds production by Bb 36Streptomyces isolate in addition to the extractionof these substances.Inhibitory compounds producing against multiresistant bacterial pathogens