full issue - Association of Biotechnology and Pharmacy

Current Trends in Biotechnology and PharmacyVol. 5 (1) 998-1003 January 2011. ISSN 0973-8916 (Print), 2230-7303 (Online)1002but not on cellulose, rafinose and meso-inositol.Melanin production on ISP-7 medium (Peptone-Iron Agar) for Bb36 isolate is negative. NaCltolerance is low (less than 7.5%) and showed nogrowth at 37°C and 45°C; however, slight ormoderate, good and very good growth wasobserved at 10°C, 22°C and 28°C, respectively(Table 3).DiscussionExperiments on optimization forantibacterial compound(s) production showed thatYeast-Extract Malt-Extract (YEME) brothmedium was the most suitable for production ofthe antibacterial compound(s) as indicated by themaximum inhibition zone diameter.The minimum inhibitory concentration(MIC) for the evaporated water-soluble ethanolextract was 5 and 10 mg/ml against S. aureusand E. coli, respectively. This shows that theantibacterial compound(s) from Streptomycesstrain (Bb 36) was less active, when it comparedto the MIC value of the tested drugs. There arevarious factors affecting the activity, the solventused for extraction may not be suitable for it orthe solvent may not properly extracted thecompound. The MIC is not constant for a givenagent, because it is affected by the nature of thetest organism used, the inoculums size, and thecomposition of the culture medium, the incubationtime, and aeration. In addition, these studies werebased on a crude form of the compounds and noton completely purified one.AcknowledgmentsThis research was funded by IslamicOrganization for Education, Culture and Science(ISESCO) (Grant No. 42/02).References1. Bibb, M. J. (2005). Regulation of secondarymetabolism in streptomycetes. Microbiology8, 208-215.2. Georgopapadakou, N. H. (1998). Antifungalmechanism of action and resistanceestablished and novel drugs. Microbiology1, 547-5573. Goodfellow, M. and O’Donnell, A.G. (1989).Search and discovery of industriallysignificant actinomycetes. In MicrobialProducts: New approaches. Ed., S.Baumberg, I. Hunter and M. Rhods,Cambridge University Press, pp. 343-383.4. Labeda, D.P. and Shearer, M.C. (1990).Isolation of actinomycetes forbiotechnological applications. In Isolation ofbiotechnological organisms from nature. Ed.,D.P. Labeda, McGrraw-Hill PublishingCompany, pp. 1-19.5. Lee, J. Y. and Hwang, B. K. (2002).Diversity of antifungal actinomycetes invarious vegetative soils of Korea.Microbiology 48, 4067-4176. Malik, M. A. (1997). Isolates of soilactinomycetes with potential for phytotoxonproduction. Journal of Chemical Ecology 23,2683-26937. Saadoun, I. and Gharaibeh, R. (2002). TheStreptomyces flora of Badia region ofJordan and its’ potential as a source ofantibiotics active against antibiotic-resistantbacteria. Journal of Arid Environments 53,365-371.8. Saadoun, I. and Muhana, A. (2008). Optimalproduction conditions, extraction, partialpurification and characterization of inhibitorycompound(s) produced by StreptomycesDs-104 isolate against multi-drug resistantCandida albicans. Current Trends inBiotechnology and Pharmacy 2, 402-420.Inhibitory compounds producing against multiresistant bacterial pathogens