TRIPZ Lentiviral Inducible shRNAmir
TRIPZ Lentiviral Inducible shRNAmir
TRIPZ Lentiviral Inducible shRNAmir
Create successful ePaper yourself
Turn your PDF publications into a flip-book with our unique Google optimized e-Paper software.
OPEN BIOSYSTEMS <strong>TRIPZ</strong> <strong>Lentiviral</strong> <strong>Inducible</strong> <strong>shRNAmir</strong>Knockdown induced by DoxycyclineBasal expression as indicated byGAPDH knockdownHigh levels of knockdown achieved after induction with doxycycline:Knockdown of indicated target genes in HEK293T cells compared to a non-silencingcontrol as determined by QPCR. Each bar represents an experimental duplicatewhile QPCR was performed in triplicate using 18SrRNA as an internal reference.HEK293T cells were transduced at an MOI=0.3, puromycin selected (5µg/ml), andinduced with doxycycline (1µg/ml). Dashed line indicates 70% knockdown.Basal expression of shRNA is not dependent on MOI: In the absence ofdoxycycline, GAPDH knockdown was measured in HEK293T cells using QPCR ascompared to a non-silencing control at increasing MOI’s. Each bar represents 4experimental replicates/3 QPCR replicates. A consistent ~20% knockdown wasobserved across a three-fold increase in MOI.Finding your <strong>TRIPZ</strong> <strong>shRNAmir</strong>of interestCatalogRHS4696Description<strong>TRIPZ</strong> lentiviral inducible <strong>shRNAmir</strong> individual clone1.Enter gene symbol(s), GenBank accession number(s), cloneIDs, unigene clusters or catalog numbers into the gene searchor into the shRNA search ( ) in the purchase grid. Resultswill be returned in a tabbed format. Click on shRNAs under theshRNAs/RNAi tab.RHS4750RHS4741<strong>TRIPZ</strong> lentiviral inducible empty vectorRNAintro <strong>TRIPZ</strong> inducible lentiviral <strong>shRNAmir</strong>transfection kit2.3.Target gene sets will be shown for all targets that have greaterthan two <strong>shRNAmir</strong>/ target. Click on the oligo ID link foradditional information including hairpin sequences.Order directly online. Constructs are shipped in 48 hours.RHS4743RHS4744Non-silencing-<strong>TRIPZ</strong> lentiviral inducible<strong>shRNAmir</strong> controlGAPDH-<strong>TRIPZ</strong> lentiviral inducible <strong>shRNAmir</strong>positive controlRHS4697<strong>TRIPZ</strong> lentiviral inducible <strong>shRNAmir</strong> libraryGo to www.openbiosystems.com to place an order or call: 1-888-412-2225 to speak with a representative.Your expert partner for Genomic, RNAi, and Proteomic resources.GENOMICS EXPRESS RNAi REPRESS PROTEOMICS DETECTFull-length cDNAsHuman ORFsPluripotency Tool KitMicroRNA-adapted shRNA librariesRNAintro shRNA transfection kitsDrosophila and C. elegans RNAi librariesCustom antibodiesCustom peptidesCatalog antibodiesLIT5022Now part of Thermo Fisher Scientific