First order Derivative Spectrophotometric Method Develop and ...

Research Article ISSN 2277-3657Available online at www.ijpras.comVolume 2, issue 1 (2013),60-64International Journal ofPharmaceutical Research &Allied SciencesFirst Order Derivative Spectrophotometric Method Developand Validate for Estimation of Bifonazole in Bulk Drug andPharmaceutical Formulation.Sayad Imran Wahab 1 *, Tadwee Imran 21 Dr. Rafiq Zakaria Campus, Maulana Azad Educational Trust`s, Y. B. Chavan College of Pharmacy,Post Box No. 33, Rauza Bagh, Aurangabad (Maharashtra) India.2 Flamingo Pharmaceutical, Mumbai, IndiaAbstract*E-mail:-imranwsayad@gmail.comSubject: Analytical chemistryA simple, rapid and sensitive reproducible First order UV Spectrophotometric method was developedfor estimation of Bifonazole (BIF) in bulk drug and pharmaceutical formulation using double beam UVspectrophotometer. Ultraviolet Spectrophotometric analysis was carried out on a Shimadzu UV 1800(Shimadzu, Japan) spectrophotometer, in a 1cm quartz cuvette. The method involved measurement ofabsorbance at wavelengths 223 nm. Beer’s law obeyed in concentration range of 1 to 12 µg/ mL. Theproposed method was applied successfully to the determination of Bifonazole in pharmaceuticalformulation with good accuracy and precision.Keyword: Spectroscopy, First Order Derivative, Bifonazole, ValidationIntroductionBifonazole is chemically 1-[(RS)-(biphenyl-4-yl) phenyl methyl]-1H-imidazole belonging toimidazole derivatives and official in theEuropean Pharmacopoeia [1]. Bifonazole isantifungal agent having broad spectrum ofactivity in vitro against dermatophytes,moulds, yeasts, dimorphic fungi and someGram-positive bacteria and use as a standardfor measuring antifungal activity of synthesizeazole derivative [2,3]. Pharmaceuticalformulations containing Bifonazole exist in theform of lotion, powder or cream[4]. So far, thecompound mentioned above was determinedusing various techniques, exemplified byelectrochemical[5], chromatographic [6,7]Spectrophotometric [8] individual andcombination with other drug. However, thereare a limited number of published papersrelated to UV spectrophotometry [9]. In thepresent study first order derivative method fora direct determination of Bifonazole in bulkdrug and pharmaceutical formulation likecream carried out. Derivativespectrophotometry is a useful technique forqualitative and quantitative analysis [10,11]and helps in reducing the effects of spectralbackground interferences. Derivativespectroscopy very useful in qualitativeanalysis, either for characterizing Materials orfor identification Derivative spectra can beobtained by optical, electronic, ormathematical methods. The advantages of themathematical techniques are that derivativespectra may be easily calculated andrecalculated with different parameters, andsmoothing techniques may be used to improvethe signal-to-noise ratio. From Fig. II clearlyindicate the common, unwanted effect inspectroscopy is baseline shift. This may ariseeither from instrument (lamp or detectorinstabilities) or sample handling (cuvetterepositioning) effects, using the first derivativespectra always eliminates such baseline shiftsand improves the accuracy of quantification[12, 13].Material and MethodInstrumentationSpectrophotometric measurements wereperformed on Shimadzu 1800spectrophotometer with 1.0 cm quartz cuvettes.60

Available online at www.ijpras.comFig. 1 Structure of BifonazoleFig. 2 Difference Absorbance and 1 st order Derivative Spectra of Gaussian bandFig. 3 First-order spectrum of Bifonazole62

Available online at www.ijpras.com0.50.450.40.350.30.250.20.150.10.0500 5 10 15Fig. 4 Calibration curve for BifonazoleParametersλmax (nm)Table 1 Optical Characteristics of Bifonazole1st Order Derivative Method223 nmLinearity (µg/ml)C µg/mLr 2 Value 0.999a. Slope(m) 0.762LOD (µg/ml) 0.3LOQ (µg/ml) 1.2Table 2 Intraday and Interdaydata of Cream formulationDrug in mgIntraday precision%COV (n =5)Interday precision %COVDay 1 Day 2 Day 31 0.46 0.388 0.276 0.156Table 3 Results of Analysis of Cream Formulation.LabelAmount of drug%Label claimRecovery % ±SDClaim in mgestimated±SD1 .00 1.00 100.00 ± 0.075 100.0063

Available online at www.ijpras.comTable 4 Result of Pharmaceutical dosage form containing BifonazoleLeval of % Concentration Concentration % Analytical recoveryRecoveryTaken(µg/mL)estimated(µg/mL)(±SD)80 1.8 1.79±0.024 99.44100 2.0 1.98±0.025 99.00120 2.2 2.21±0.021 100.45ConclusionThe results of our study indicate that theproposed UV spectroscopic methods aresimple, rapid, precise and accurate. Thedeveloped UV spectroscopic methods werefound suitable for determination of BIF in bulkdrug and in marketed Cream formulationwithout any interference from the excipients.Statistical analysis proves that, these methodsare repeatable and selective for the analysis ofBIF. It can therefore be concluded that use ofthese methods can save much time and moneyand it can be used in laboratories withaccuracy.Acknowledgement:The authors are very much thankful to theChairman, Pdmashree Mrs. Fatma RafiqZakaria, Maulana Azad Educational Trust andPrincipal Dr. M. H. Dehghan Y. B. ChavanCollege of Pharmacy, Aurangabad forproviding necessary facilities for the projectwork.“Cite this article”I. W. Sayad, I. K. Tadwee“First orderDerivative Spectrophotometric Method Developand Validate for Estimation of Bifonazole in BulkDrug Formulation and PharmaceuticalFormulation” Int. J. of Pharm. Res. & All.Sci.2013; Volume 2, Issue 1,60-64Reference:1. European Pharmacopoeia 5.0, Vol II, TheEuropean Pharmacopoeia, France (2005) 1098.2. Lackner, T. E., Clissold, S. P., Bifonazole. Areview of its antimicrobial activity andtherapeutic use in superficial mycoses. Drugs,1989;38(2):204-25.3. http://www.mims.com/India/drug/info/MYCOSPOR/MYCOSPOR%20cream?q=bifonazoleaccessed on 28/09/2012.4. Mushik, V., Tkach, V.I., Karandeeva, N.I.,Glukhova, O.I., Tsyganok, L.P.Electrochemical and analytical properties ofsolid-contact ion-selective electrodesreversible to the imidazole derivativesclotrimazole and bifonazole. ZhurnalAnaliticheskoi Khimii, 1998. 53(2);1110-125. Kadenatsi, I. B., Agapitova, I. V., Shustova, L.V., Salib, I., Dombrovskil V. S., Gagaeva, E.V., Kuleshova, E. E., Arzamastsev, A. P.,Firsov, A., Determination of Bifonazole usingHPLC in pharmacokinetic studies. AntibiotKhimioter. 1996;41(5); 19-24.6. Guo, X. J., Wei, Z. Y., Li, F., M.,Determination of Bifonazole in cream by highperformance liquid chromatography. Se Pu.2001;19(3):;279-80.7. Cakar Mira, M., Popovic, G.,Spectrophotometric determination ofBifonazole and Clotrimazole in lotionsBicutrin® and Canesten®.Arhiv za farmaciju.2004, 54(1-2);1-138. Radoslaw J. E., Karjek J., Determination ofAzole Antifungal Medicines Using Zero-Orderand Derivative UV Spectrophotometry, ActaPolonica-Drug Research, 2009;66(1), 19-24.9. Koytchev, R., Ozalp, Y., Erenmemisoglu, A.,Van Der Meer, M. J., Alpan, R.S.,Arzneimittel-Forschung / Drug Research ,2004, 54 (9 A) , 629-633.10. Jun-Sheng, Yu; Zu-Xun, Zhang, Journal ofElectro analytical Chemistry, 1996, 403, 1-9.11. Beckett, A.H. and Stenlake, J. B., In; Practicalpharmaceutical chemistry, 3rd edition, vol.2,CBS publishers and distributor, NewDelhi,1998, 275.64