Plasma membrane calcium ATPases are important components of ...

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Frei dit Frey et al.12345deviation (n = 3 biological repeats) is indicated. All genes tested are significantlydifferentially expressed (Student’s T-test : p< 0.05) unless for AT5G64870.Table S3. List of all oligonucleotides used in this study.26
Figure 1.AFLS2-YFPnFLS2-YFPcFLS2-YFPnACA8-YFPcFLS2-YFPnACA12-YFPcBRI1-YFPnBRI1-YFPcBRI1-YFPnACA8-YFPcBRI1-YFPnACA12-YFPcCLV1-YFPnCLV1-YFPcCLV1-YFPnACA8-YFPcCLV1-YFPnACA12-YFPcB ACA8-CFP2.2 nsACA8-CFPFLS2-YFP1.8 nsFLS2-CFPFLS2-CFPACA8-YFP1850 ps 2500 psFigure 1. FLS2 interacts with ACA8 in planta. A. Bimolecular fluorescencecomplementation. Representative micrographs show YFP signals of epidermal cells fromN. benthamiana leaves transformed with plasmids coding for the indicated constructs. N-terminal YFP fragment = YFPn; C-terminal YFP fragment = YFPc. Scale bar = 100 µm. B.FRET-FLIM measurements. Micrographs show representative false colour-codedfluorescence lifetime images of Arabidopsis protoplasts transfected with plasmids codingfor the indicated constructs. Lower fluorescence lifetimes indicate proximity of the twofluorescence proteins. Scale bar = 2 µm. Similar results were obtained at least in threeindependent experiments.
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Page 28 and 29: Figure 2.ACol-0aca8aca8 aca10ACA8-G
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Page 32 and 33: Supplementary figure 1.AACA4 ACA11A
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Plasma membrane calcium ATPases are important components of ...

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