PDMS Chip Interface - Dolomite Microfluidics

PDMS Chip Interfaceproduct datasheetpageDescription 2Main Benefits 3Specification 4Cross-Sectional View 5Geometry 6Design Guidelines 8Fabrication Guidelines 10PDMS Chip Interface Starter Kit 11Custom Options 11

The Dolomite Centre LtdPart namePart numberPDMS Chip Interface 3200232PDMS Chip Slide 50 x 25 mm (pack of 10) 3200264PDMS Chip Slide 75 x 25 mm (pack of 10) 3200275PDMS Chip Interface Starter Kit 3200273DescriptionThe PDMS Chip Interface (Part No. 3200232) allows users to create fast and reliablefluidic connections to PDMS-on-glass devices. The interface is designed to be used with aPDMS device (fabricated by the user) mounted to a PDMS Chip Slide with pre-drilledholes for fluidic connection (2 sizes available).Designing a PDMS device to fit the Dolomite format is quick and easy when following thedesign guidelines. Fabrication can be carried out using existing methods and equipment.The PDMS Chip Interface is compatible with the Multiflux® Linear Connector 4-way (PartNo. 3000024) to provide 4 or 8 fluidic connections from 1/16” OD tubing to the PDMSchip.PDMS Chip Interface (Part No. 3200232)PDMS Chip Slide (Part No. 3200275)Full assembly of the PDMS Chip with twoPDMS Chip Interfaces and two LinearConnectors 4-way (Part No. 3000024)Full assembly of the PDMS Chip with onePDMS Chip Interface and one LinearConnector 4-way (Part No. 3000024)PDMS_chip_interface_product_datasheet Page 2 of 12

The Dolomite Centre LtdMain Benefits• Robust and reliable connections• Provides a standard format for PDMS chip connections• Ideal for research use• Time savings in fabrication: no requirement to punch holes in PDMS• Time savings in use: no downtime due to leaks• Extends chip lifetimes• Widens applications for PDMS chips outside of research labs• Quick connect/disconnect• Low dead volume• Good microscope access• Accommodates wide range of PDMS device heightsPDMS_chip_interface_product_datasheet Page 3 of 12

The Dolomite Centre LtdSpecificationSpecification PDMS Chip Interface (Part No. 3200232)Number of fluidic connections 4 or 8*Compatible glass slide dimensions 50 x 25 x 1.3mm or 75 x 25 x 1.3mm (L x W x H)Glass slide hole sizePDMS chip thickness optionsPDMS chip width optionsPDMS chip length optionsOutside diameter of connection tubingInside diameter of connection tubingConnection tubing materialOperating pressureOperating temperature range of sealWetted materialsØ0.5mmUp to 6mmUp to 15mm10 – 44mm (50 x 25mm slide)10 – 69mm (75 x 25mm slide)1.6mm (1/16inch)0.25, 0.5 or 0.8mmPTFE, FEP5bar**-15 to 150°C***Glass, PDMS, PTFE (or FEP), and perfluoroelastomerConnector materials PEEK, Stainless Steel 316*8 fluidic connections requires 2 x PDMS Chip Interface (Part No. 3200232) and 2 x Multiflux®Linear Connector 4-way (Part No. 3000024)**Based on tests at 21C, PDMS chip may delaminate at lower pressure***Assumes external surfaces of connector are at room temperature and tube I.D.

The Dolomite Centre LtdCross-sectional viewLinear Connector 4-way(Part No. 3000024)Glass slide 50 x 25 mm(Part No. 3200264)Left: Cross-sectional viewwith PDMS Chip Slide 50 x 25mm (Part No. 3200264)Below: Cross-sectional viewwith PDMS Chip Slide 75 x 25mm (Part No. 3200275)PDMS Chip Interface (Part No. 3200232)PDMS microfluidic deviceLinear Connector 4-way(Part No. 3000024)Glass slide 75 x 25 mm(Part No. 3200275)PDMS Chip Interface (Part No. 3200232)PDMS microfluidic devicePTFE or FEP tubingPerfluoroelastomer sealHole through glass slideDetailed View of Sealing InterfaceMicrochannel in PDMSPDMS_chip_interface_product_datasheet Page 5 of 12

The Dolomite Centre LtdGeometryGeometry of PDMS Chip with one PDMS Chip Interface and one Linear Connector 4-way (Part No. 3000024)PDMS_chip_interface_product_datasheet Page 6 of 12

The Dolomite Centre LtdGeometry of PDMS Chip with two PDMS Chip Interfaces and two Linear Connectors 4-way (Part No. 3000024)PDMS_chip_interface_product_datasheet Page 7 of 12

The Dolomite Centre LtdDesign GuidelinesIn order to design a compatible PDMS device for use with the PDMS Chip Interface, it isnecessary to follow 5 simple steps:1. Decide if you require 4 fluidic connections or 8 fluidic connections.a. 4 connections can be made with one PDMS Chip Interface and one LinearConnector 4-way. Two chips can be mounted to each slide.b. 8 connections can be made with two PDMS Chip Interfaces and two LinearConnectors 4-way. One chip can be mounted to each slide.2. Decide if your device length fits onto the standard 50 x 25 mm slide or if yourequire the longer 75 x 25 mm slide.3. Design pad features in PDMS (recommended Ø1 mm) at a pitch of 3 mm to alignwith Ø0.5 holes in glass slide. These are the inlet and outlet ports.4. Design outer dimensions of PDMS device ensuring that the boundaries are withinthe maximum width and length specified.a. Maximum width is 15 mm. The maximum distance from outside port toPDMS side edges is 3 mm.b. Maximum length depends on the number of fluidic connections (step 1)and the slide size selected (step 2). The maximum distance from inlet andoutlet ports to PDMS back edge is 3 mm.5. Design channel features in PDMS ensuring that all features to be viewed arepositioned at least 3 mm from the inlet and outlet ports. Visible features should beoutside of the red box in the diagram below.PDMS_chip_interface_product_datasheet Page 8 of 12

The Dolomite Centre LtdKey design dimensions of PDMS replica if 4 fluidic connections are required and2 chips of equal length are mounted onto the PDMS Chip SlideKey design dimensions of PDMS replica if 8 fluidic connections are required (1 chipmounted onto PDMS Chip Slide)PDMS_chip_interface_product_datasheet Page 9 of 12

The Dolomite Centre LtdFabrication GuidelinesFabrication of the PDMS device can be carried out using existing soft lithographicmethods and equipment:1. Fabricate a photoresist master (spin coat photoresist onto a silicon wafer, exposeand develop).2. Degas the PDMS mixture. Then cast the PDMS onto the master, cure and peelthe wafer of PDMS replicas from the photoresist master. The PDMS height shouldbe no greater than 6 mm.3. Dice the wafer of PDMS replicas into individual chips ensuring that outerdimensions are within the allowed size.4. Ensure that bonding surfaces of the glass and PDMS are clean then expose bothsurfaces to O 2 plasma.5. Align PDMS pad features to holes in the glass slide by eye or using lowmagnification eyepiece or viewer. Apply light pressure to create bond.6. The completed PDMS chip can then be used with the PDMS Chip Interface.Described above is a typical soft lithography process, but alternative methods may beused for the creation of channel features in the PDMS.Example of PDMS replica mounted onto PDMS Chip SlidePDMS_chip_interface_product_datasheet Page 10 of 12

The Dolomite Centre LtdPDMS Chip Interface Starter KitThe PDMS Chip Interface Starter Kit (Part No. 3200273) contains the requiredcomponents to start using the Multiflux® PDMS chip connection system.The starter kit includes:• 1 x PDMS Chip Interface – Part No. 3200232• 1 x PDMS Chip Slide 50 x 25mm (pack of 10) – Part No. 3200264• 1 x Multiflux® Linear Connector 4-way – Part No. 3000024• 1 x Fluid Tube PTFE 1/16” OD, 0.5mm I.D, 10m Length – Part No. 3200067PDMS Chip Interface Starter Kit (Part No. 3200273)Custom OptionsDolomite can offer custom connection systems for individual applications including thinlayer glass slides for high magnification microscopy. Microscope holders are alsoavailable on request. The range of Dolomite services available covers all aspects of thedevelopment process from concept studies and feasibility testing to the design ofcommercial instruments. Please contact Dolomite to discuss your application.PDMS_chip_interface_product_datasheet Page 11 of 12

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