Quenching + FRET

The overlap integral J is defined by:J=∞∫0fSpectral Overlap Integral J4D( λ)εA(λ)λ dλDonorAbsorbanceFluorescenceWhere λ is the wavelength of thelight, ε A (λ) is the molar extinctioncoefficient at that wavelength andƒ D (λ) is the fluorescence spectrum ofthe donor normalized on thewavelength scale:fD( λ)=∞∫0FFDλDλ( λ)( λ)dλWhere F D λ (λ) is the donorfluorescence per unit wavelengthintervalAcceptorOverlapAbsorbanceFluorescencePM